This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.30 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 84,125 (3.4%) Reads that were too short: 20,483 (0.8%) Reads written (passing filters): 63,642 (2.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 13,311,119 bp (1.8%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 84125 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 496 601.0 0 496 7 10726 601.0 0 10726 8 294 601.0 0 294 9 10846 601.0 0 10846 10 83 601.0 0 83 11 75 601.0 0 75 12 78 601.0 0 78 13 96 601.0 0 96 14 74 601.0 0 74 15 71 601.0 0 71 16 81 601.0 0 81 17 87 601.0 0 87 18 77 601.0 0 77 19 82 601.0 0 82 20 81 601.0 0 81 21 81 601.0 0 81 22 103 601.0 0 103 23 74 601.0 0 74 24 106 601.0 0 106 25 100 601.0 0 100 26 108 601.0 0 108 27 87 601.0 0 87 28 85 601.0 0 85 29 97 601.0 0 97 30 97 601.0 0 97 31 370 601.0 0 370 32 179 601.0 0 179 33 222 601.0 0 222 34 83 601.0 0 83 35 90 601.0 0 90 36 110 601.0 0 110 37 194 601.0 0 194 38 85 601.0 0 85 39 100 601.0 0 100 40 67 601.0 0 67 41 91 601.0 0 91 42 68 601.0 0 68 43 60 601.0 0 60 44 81 601.0 0 81 45 68 601.0 0 68 46 81 601.0 0 81 47 70 601.0 0 70 48 71 601.0 0 71 49 66 601.0 0 66 50 155 601.0 0 155 51 144 601.0 0 144 52 122 601.0 0 122 53 75 601.0 0 75 54 65 601.0 0 65 55 70 601.0 0 70 56 88 601.0 0 88 57 73 601.0 0 73 58 88 601.0 0 88 59 83 601.0 0 83 60 70 601.0 0 70 61 69 601.0 0 69 62 80 601.0 0 80 63 95 601.0 0 95 64 75 601.0 0 75 65 107 601.0 0 107 66 109 601.0 0 109 67 180 601.0 0 180 68 723 601.0 0 723 69 509 601.0 0 509 70 574 601.0 0 574 71 78 601.0 0 78 72 81 601.0 0 81 73 88 601.0 0 88 74 90 601.0 0 90 75 107 601.0 0 107 76 87 601.0 0 87 77 77 601.0 0 77 78 69 601.0 0 69 79 166 601.0 0 166 80 326 601.0 0 326 81 162 601.0 0 162 82 76 601.0 0 76 83 100 601.0 0 100 84 116 601.0 0 116 85 98 601.0 0 98 86 89 601.0 0 89 87 80 601.0 0 80 88 110 601.0 0 110 89 96 601.0 0 96 90 57 601.0 0 57 91 102 601.0 0 102 92 65 601.0 0 65 93 62 601.0 0 62 94 70 601.0 0 70 95 85 601.0 0 85 96 81 601.0 0 81 97 94 601.0 0 94 98 199 601.0 0 199 99 165 601.0 0 165 100 146 601.0 0 146 101 101 601.0 0 101 102 80 601.0 0 80 103 86 601.0 0 86 104 99 601.0 0 99 105 77 601.0 0 77 106 99 601.0 0 99 107 82 601.0 0 82 108 80 601.0 0 80 109 110 601.0 0 110 110 98 601.0 0 98 111 95 601.0 0 95 112 97 601.0 0 97 113 86 601.0 0 86 114 88 601.0 0 88 115 87 601.0 0 87 116 619 601.0 0 619 117 378 601.0 0 378 118 253 601.0 0 253 119 108 601.0 0 108 120 122 601.0 0 122 121 225 601.0 0 225 122 182 601.0 0 182 123 142 601.0 0 142 124 197 601.0 0 197 125 102 601.0 0 102 126 151 601.0 0 151 127 185 601.0 0 185 128 163 601.0 0 163 129 104 601.0 0 104 130 192 601.0 0 192 131 209 601.0 0 209 132 169 601.0 0 169 133 414 601.0 0 414 134 695 601.0 0 695 135 834 601.0 0 834 136 352 601.0 0 352 137 135 601.0 0 135 138 268 601.0 0 268 139 160 601.0 0 160 140 102 601.0 0 102 141 100 601.0 0 100 142 164 601.0 0 164 143 125 601.0 0 125 144 236 601.0 0 236 145 141 601.0 0 141 146 141 601.0 0 141 147 100 601.0 0 100 148 160 601.0 0 160 149 100 601.0 0 100 150 137 601.0 0 137 151 187 601.0 0 187 152 566 601.0 0 566 153 418 601.0 0 418 154 455 601.0 0 455 155 264 601.0 0 264 156 331 601.0 0 331 157 321 601.0 0 321 158 101 601.0 0 101 159 336 601.0 0 336 160 113 601.0 0 113 161 284 601.0 0 284 162 125 601.0 0 125 163 79 601.0 0 79 164 854 601.0 0 854 165 133 601.0 0 133 166 279 601.0 0 279 167 283 601.0 0 283 168 327 601.0 0 327 169 282 601.0 0 282 170 103 601.0 0 103 171 117 601.0 0 117 172 92 601.0 0 92 173 113 601.0 0 113 174 200 601.0 0 200 175 889 601.0 0 889 176 527 601.0 0 527 177 436 601.0 0 436 178 247 601.0 0 247 179 228 601.0 0 228 180 1115 601.0 0 1115 181 1209 601.0 0 1209 182 848 601.0 0 848 183 289 601.0 0 289 184 203 601.0 0 203 185 146 601.0 0 146 186 231 601.0 0 231 187 184 601.0 0 184 188 137 601.0 0 137 189 98 601.0 0 98 190 73 601.0 0 73 191 109 601.0 0 109 192 108 601.0 0 108 193 112 601.0 0 112 194 179 601.0 0 179 195 556 601.0 0 556 196 594 601.0 0 594 197 885 601.0 0 885 198 484 601.0 0 484 199 163 601.0 0 163 200 2112 601.0 0 2112 201 2770 601.0 0 2770 202 1904 601.0 0 1904 203 345 601.0 0 345 204 68 601.0 0 68 205 97 601.0 0 97 206 73 601.0 0 73 207 103 601.0 0 103 208 95 601.0 0 95 209 102 601.0 0 102 210 74 601.0 0 74 211 95 601.0 0 95 212 112 601.0 0 112 213 104 601.0 0 104 214 91 601.0 0 91 215 89 601.0 0 89 216 106 601.0 0 106 217 103 601.0 0 103 218 85 601.0 0 85 219 88 601.0 0 88 220 116 601.0 0 116 221 316 601.0 0 316 222 286 601.0 0 286 223 227 601.0 0 227 224 112 601.0 0 112 225 104 601.0 0 104 226 157 601.0 0 157 227 122 601.0 0 122 228 62 601.0 0 62 229 97 601.0 0 97 230 105 601.0 0 105 231 85 601.0 0 85 232 99 601.0 0 99 233 87 601.0 0 87 234 79 601.0 0 79 235 105 601.0 0 105 236 106 601.0 0 106 237 109 601.0 0 109 238 177 601.0 0 177 239 161 601.0 0 161 240 160 601.0 0 160 241 192 601.0 0 192 242 266 601.0 0 266 243 189 601.0 0 189 244 119 601.0 0 119 245 97 601.0 0 97 246 89 601.0 0 89 247 80 601.0 0 80 248 117 601.0 0 117 249 323 601.0 0 323 250 336 601.0 0 336 251 204 601.0 0 204 252 126 601.0 0 126 253 238 601.0 0 238 254 177 601.0 0 177 255 479 601.0 0 479 256 266 601.0 0 266 257 157 601.0 0 157 258 254 601.0 0 254 259 314 601.0 0 314 260 296 601.0 0 296 261 144 601.0 0 144 262 164 601.0 0 164 263 457 601.0 0 457 264 1442 601.0 0 1442 265 702 601.0 0 702 266 238 601.0 0 238 267 85 601.0 0 85 268 158 601.0 0 158 269 187 601.0 0 187 270 145 601.0 0 145 271 120 601.0 0 120 272 132 601.0 0 132 273 155 601.0 0 155 274 185 601.0 0 185 275 261 601.0 0 261 276 1375 601.0 0 1375 277 611 601.0 0 611 278 283 601.0 0 283 279 68 601.0 0 68 280 78 601.0 0 78 281 68 601.0 0 68 282 83 601.0 0 83 283 118 601.0 0 118 284 71 601.0 0 71 285 101 601.0 0 101 286 102 601.0 0 102 287 102 601.0 0 102 288 102 601.0 0 102 289 114 601.0 0 114 290 197 601.0 0 197 291 138 601.0 0 138 292 123 601.0 0 123 293 116 601.0 0 116 294 113 601.0 0 113 295 90 601.0 0 90 296 115 601.0 0 115 297 118 601.0 0 118 298 180 601.0 0 180 299 161 601.0 0 161 300 173 601.0 0 173 301 183 601.0 0 183 Finished in 2.88 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 63,642 Reads with adapters: 10,682 (16.8%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 10,618 (16.7%) Total basepairs processed: 13,311,119 bp Total written (filtered): 2,907,637 bp (21.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10682 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 7 0.0 3 0 0 1 4 2 19 57 0.0 3 0 37 8 5 7 20 10467 0.0 4 9588 686 143 41 9 21 33 0.0 4 7 24 1 0 1 22 10 0.0 4 4 1 3 2 23 25 0.0 4 2 1 1 19 2 25 1 0.0 4 1 26 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 36 2 0.0 4 0 0 0 0 2 40 4 0.0 4 0 0 0 0 4 41 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 1 70 2 0.0 4 0 0 2 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 111 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 170 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 210 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 1 1 212 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 1 1 234 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 1 273 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 10,618 Reads with adapters: 632 (6.0%) Reads written (passing filters): 10,618 (100.0%) Total basepairs processed: 2,907,637 bp Total written (filtered): 2,896,073 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 632 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.2% C: 18.4% G: 65.5% T: 4.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 85 165.9 0 85 4 11 41.5 0 11 5 1 10.4 0 1 9 13 0.0 0 3 10 10 6 0.0 1 2 4 11 281 0.0 1 16 265 12 202 0.0 1 12 190 15 7 0.0 1 1 6 16 1 0.0 1 0 1 152 1 0.0 1 0 1 179 1 0.0 1 1 210 5 0.0 1 2 3 225 16 0.0 1 14 2 226 1 0.0 1 1 240 1 0.0 1 1