This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.38 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 64,277 (2.6%) Reads that were too short: 12,619 (0.5%) Reads written (passing filters): 51,658 (2.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 11,677,151 bp (1.6%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 64277 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 370 601.0 0 370 7 13000 601.0 0 13000 8 183 601.0 0 183 9 10089 601.0 0 10089 10 74 601.0 0 74 11 56 601.0 0 56 12 48 601.0 0 48 13 64 601.0 0 64 14 58 601.0 0 58 15 58 601.0 0 58 16 81 601.0 0 81 17 77 601.0 0 77 18 62 601.0 0 62 19 39 601.0 0 39 20 43 601.0 0 43 21 65 601.0 0 65 22 64 601.0 0 64 23 45 601.0 0 45 24 60 601.0 0 60 25 53 601.0 0 53 26 59 601.0 0 59 27 49 601.0 0 49 28 62 601.0 0 62 29 68 601.0 0 68 30 99 601.0 0 99 31 120 601.0 0 120 32 106 601.0 0 106 33 97 601.0 0 97 34 72 601.0 0 72 35 45 601.0 0 45 36 81 601.0 0 81 37 143 601.0 0 143 38 77 601.0 0 77 39 76 601.0 0 76 40 62 601.0 0 62 41 77 601.0 0 77 42 82 601.0 0 82 43 47 601.0 0 47 44 75 601.0 0 75 45 77 601.0 0 77 46 60 601.0 0 60 47 60 601.0 0 60 48 61 601.0 0 61 49 84 601.0 0 84 50 79 601.0 0 79 51 119 601.0 0 119 52 70 601.0 0 70 53 59 601.0 0 59 54 79 601.0 0 79 55 63 601.0 0 63 56 80 601.0 0 80 57 44 601.0 0 44 58 66 601.0 0 66 59 81 601.0 0 81 60 67 601.0 0 67 61 59 601.0 0 59 62 68 601.0 0 68 63 92 601.0 0 92 64 51 601.0 0 51 65 83 601.0 0 83 66 105 601.0 0 105 67 110 601.0 0 110 68 884 601.0 0 884 69 546 601.0 0 546 70 668 601.0 0 668 71 62 601.0 0 62 72 71 601.0 0 71 73 64 601.0 0 64 74 60 601.0 0 60 75 61 601.0 0 61 76 39 601.0 0 39 77 61 601.0 0 61 78 62 601.0 0 62 79 69 601.0 0 69 80 256 601.0 0 256 81 92 601.0 0 92 82 69 601.0 0 69 83 62 601.0 0 62 84 67 601.0 0 67 85 76 601.0 0 76 86 57 601.0 0 57 87 49 601.0 0 49 88 80 601.0 0 80 89 68 601.0 0 68 90 70 601.0 0 70 91 78 601.0 0 78 92 55 601.0 0 55 93 56 601.0 0 56 94 71 601.0 0 71 95 69 601.0 0 69 96 71 601.0 0 71 97 66 601.0 0 66 98 87 601.0 0 87 99 83 601.0 0 83 100 71 601.0 0 71 101 83 601.0 0 83 102 68 601.0 0 68 103 89 601.0 0 89 104 82 601.0 0 82 105 73 601.0 0 73 106 56 601.0 0 56 107 74 601.0 0 74 108 73 601.0 0 73 109 71 601.0 0 71 110 72 601.0 0 72 111 61 601.0 0 61 112 54 601.0 0 54 113 75 601.0 0 75 114 70 601.0 0 70 115 61 601.0 0 61 116 217 601.0 0 217 117 107 601.0 0 107 118 100 601.0 0 100 119 92 601.0 0 92 120 70 601.0 0 70 121 124 601.0 0 124 122 103 601.0 0 103 123 81 601.0 0 81 124 140 601.0 0 140 125 73 601.0 0 73 126 118 601.0 0 118 127 169 601.0 0 169 128 121 601.0 0 121 129 59 601.0 0 59 130 97 601.0 0 97 131 110 601.0 0 110 132 108 601.0 0 108 133 223 601.0 0 223 134 386 601.0 0 386 135 606 601.0 0 606 136 200 601.0 0 200 137 116 601.0 0 116 138 251 601.0 0 251 139 109 601.0 0 109 140 68 601.0 0 68 141 67 601.0 0 67 142 135 601.0 0 135 143 103 601.0 0 103 144 137 601.0 0 137 145 82 601.0 0 82 146 108 601.0 0 108 147 77 601.0 0 77 148 149 601.0 0 149 149 72 601.0 0 72 150 113 601.0 0 113 151 106 601.0 0 106 152 580 601.0 0 580 153 388 601.0 0 388 154 399 601.0 0 399 155 113 601.0 0 113 156 129 601.0 0 129 157 151 601.0 0 151 158 73 601.0 0 73 159 386 601.0 0 386 160 95 601.0 0 95 161 332 601.0 0 332 162 97 601.0 0 97 163 100 601.0 0 100 164 942 601.0 0 942 165 92 601.0 0 92 166 274 601.0 0 274 167 261 601.0 0 261 168 259 601.0 0 259 169 252 601.0 0 252 170 77 601.0 0 77 171 74 601.0 0 74 172 66 601.0 0 66 173 68 601.0 0 68 174 112 601.0 0 112 175 433 601.0 0 433 176 263 601.0 0 263 177 208 601.0 0 208 178 128 601.0 0 128 179 125 601.0 0 125 180 608 601.0 0 608 181 888 601.0 0 888 182 573 601.0 0 573 183 198 601.0 0 198 184 131 601.0 0 131 185 101 601.0 0 101 186 166 601.0 0 166 187 121 601.0 0 121 188 75 601.0 0 75 189 63 601.0 0 63 190 101 601.0 0 101 191 80 601.0 0 80 192 68 601.0 0 68 193 80 601.0 0 80 194 129 601.0 0 129 195 214 601.0 0 214 196 278 601.0 0 278 197 385 601.0 0 385 198 252 601.0 0 252 199 80 601.0 0 80 200 1122 601.0 0 1122 201 1306 601.0 0 1306 202 829 601.0 0 829 203 160 601.0 0 160 204 56 601.0 0 56 205 89 601.0 0 89 206 73 601.0 0 73 207 78 601.0 0 78 208 75 601.0 0 75 209 75 601.0 0 75 210 48 601.0 0 48 211 76 601.0 0 76 212 98 601.0 0 98 213 80 601.0 0 80 214 84 601.0 0 84 215 50 601.0 0 50 216 67 601.0 0 67 217 60 601.0 0 60 218 66 601.0 0 66 219 74 601.0 0 74 220 83 601.0 0 83 221 114 601.0 0 114 222 148 601.0 0 148 223 93 601.0 0 93 224 102 601.0 0 102 225 161 601.0 0 161 226 266 601.0 0 266 227 131 601.0 0 131 228 66 601.0 0 66 229 87 601.0 0 87 230 73 601.0 0 73 231 60 601.0 0 60 232 84 601.0 0 84 233 92 601.0 0 92 234 67 601.0 0 67 235 68 601.0 0 68 236 81 601.0 0 81 237 87 601.0 0 87 238 126 601.0 0 126 239 98 601.0 0 98 240 106 601.0 0 106 241 107 601.0 0 107 242 99 601.0 0 99 243 98 601.0 0 98 244 75 601.0 0 75 245 71 601.0 0 71 246 62 601.0 0 62 247 66 601.0 0 66 248 82 601.0 0 82 249 147 601.0 0 147 250 130 601.0 0 130 251 124 601.0 0 124 252 88 601.0 0 88 253 245 601.0 0 245 254 113 601.0 0 113 255 390 601.0 0 390 256 117 601.0 0 117 257 91 601.0 0 91 258 238 601.0 0 238 259 286 601.0 0 286 260 260 601.0 0 260 261 74 601.0 0 74 262 103 601.0 0 103 263 161 601.0 0 161 264 619 601.0 0 619 265 323 601.0 0 323 266 151 601.0 0 151 267 68 601.0 0 68 268 94 601.0 0 94 269 98 601.0 0 98 270 82 601.0 0 82 271 66 601.0 0 66 272 81 601.0 0 81 273 78 601.0 0 78 274 89 601.0 0 89 275 107 601.0 0 107 276 604 601.0 0 604 277 330 601.0 0 330 278 170 601.0 0 170 279 50 601.0 0 50 280 58 601.0 0 58 281 68 601.0 0 68 282 67 601.0 0 67 283 61 601.0 0 61 284 70 601.0 0 70 285 86 601.0 0 86 286 92 601.0 0 92 287 62 601.0 0 62 288 80 601.0 0 80 289 72 601.0 0 72 290 81 601.0 0 81 291 71 601.0 0 71 292 97 601.0 0 97 293 82 601.0 0 82 294 134 601.0 0 134 295 72 601.0 0 72 296 103 601.0 0 103 297 89 601.0 0 89 298 177 601.0 0 177 299 146 601.0 0 146 300 130 601.0 0 130 301 153 601.0 0 153 Finished in 2.14 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 51,658 Reads with adapters: 10,629 (20.6%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 10,579 (20.5%) Total basepairs processed: 11,677,151 bp Total written (filtered): 2,900,022 bp (24.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10629 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 45 0.0 3 0 45 17 10243 0.0 3 10048 175 7 13 18 26 0.0 3 4 22 19 11 0.0 3 2 2 7 20 201 0.0 3 191 4 0 6 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 36 10 0.0 3 0 0 0 10 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 4 0.0 3 0 0 0 4 47 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 9 0.0 3 0 0 0 9 60 1 0.0 3 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 8 0.0 3 0 0 0 8 83 2 0.0 3 1 1 87 1 0.0 3 1 92 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 2 1 94 2 0.0 3 1 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 9 0.0 3 0 0 0 9 110 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 8 0.0 3 0 0 0 8 164 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 10,579 Reads with adapters: 260 (2.5%) Reads written (passing filters): 10,579 (100.0%) Total basepairs processed: 2,900,022 bp Total written (filtered): 2,899,236 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 260 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.8% C: 3.1% G: 54.6% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 254 165.3 0 254 4 6 41.3 0 6