This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.65 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 366,893 (14.9%) Reads that were too short: 22,075 (0.9%) Reads written (passing filters): 344,818 (14.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 97,480,369 bp (13.2%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 366893 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1844 601.0 0 1844 7 108458 601.0 0 108458 8 3514 601.0 0 3514 9 80524 601.0 0 80524 10 1029 601.0 0 1029 11 1261 601.0 0 1261 12 26819 601.0 0 26819 13 82684 601.0 0 82684 14 13861 601.0 0 13861 15 59 601.0 0 59 16 54 601.0 0 54 17 52 601.0 0 52 18 52 601.0 0 52 19 41 601.0 0 41 20 47 601.0 0 47 21 42 601.0 0 42 22 57 601.0 0 57 23 53 601.0 0 53 24 52 601.0 0 52 25 45 601.0 0 45 26 69 601.0 0 69 27 146 601.0 0 146 28 124 601.0 0 124 29 65 601.0 0 65 30 50 601.0 0 50 31 44 601.0 0 44 32 53 601.0 0 53 33 48 601.0 0 48 34 71 601.0 0 71 35 42 601.0 0 42 36 57 601.0 0 57 37 54 601.0 0 54 38 44 601.0 0 44 39 52 601.0 0 52 40 90 601.0 0 90 41 44 601.0 0 44 42 51 601.0 0 51 43 67 601.0 0 67 44 187 601.0 0 187 45 290 601.0 0 290 46 44 601.0 0 44 47 39 601.0 0 39 48 48 601.0 0 48 49 51 601.0 0 51 50 51 601.0 0 51 51 50 601.0 0 50 52 32 601.0 0 32 53 60 601.0 0 60 54 53 601.0 0 53 55 40 601.0 0 40 56 30 601.0 0 30 57 55 601.0 0 55 58 58 601.0 0 58 59 46 601.0 0 46 60 42 601.0 0 42 61 59 601.0 0 59 62 43 601.0 0 43 63 61 601.0 0 61 64 41 601.0 0 41 65 60 601.0 0 60 66 66 601.0 0 66 67 50 601.0 0 50 68 55 601.0 0 55 69 36 601.0 0 36 70 55 601.0 0 55 71 41 601.0 0 41 72 49 601.0 0 49 73 70 601.0 0 70 74 72 601.0 0 72 75 47 601.0 0 47 76 48 601.0 0 48 77 66 601.0 0 66 78 68 601.0 0 68 79 56 601.0 0 56 80 58 601.0 0 58 81 57 601.0 0 57 82 47 601.0 0 47 83 53 601.0 0 53 84 47 601.0 0 47 85 39 601.0 0 39 86 69 601.0 0 69 87 61 601.0 0 61 88 57 601.0 0 57 89 84 601.0 0 84 90 106 601.0 0 106 91 79 601.0 0 79 92 123 601.0 0 123 93 69 601.0 0 69 94 79 601.0 0 79 95 58 601.0 0 58 96 43 601.0 0 43 97 53 601.0 0 53 98 67 601.0 0 67 99 54 601.0 0 54 100 78 601.0 0 78 101 66 601.0 0 66 102 58 601.0 0 58 103 68 601.0 0 68 104 529 601.0 0 529 105 396 601.0 0 396 106 307 601.0 0 307 107 106 601.0 0 106 108 125 601.0 0 125 109 96 601.0 0 96 110 162 601.0 0 162 111 146 601.0 0 146 112 175 601.0 0 175 113 89 601.0 0 89 114 118 601.0 0 118 115 91 601.0 0 91 116 116 601.0 0 116 117 109 601.0 0 109 118 58 601.0 0 58 119 79 601.0 0 79 120 87 601.0 0 87 121 60 601.0 0 60 122 73 601.0 0 73 123 70 601.0 0 70 124 157 601.0 0 157 125 152 601.0 0 152 126 653 601.0 0 653 127 340 601.0 0 340 128 779 601.0 0 779 129 344 601.0 0 344 130 82 601.0 0 82 131 74 601.0 0 74 132 97 601.0 0 97 133 88 601.0 0 88 134 87 601.0 0 87 135 108 601.0 0 108 136 108 601.0 0 108 137 136 601.0 0 136 138 1014 601.0 0 1014 139 322 601.0 0 322 140 692 601.0 0 692 141 279 601.0 0 279 142 202 601.0 0 202 143 71 601.0 0 71 144 121 601.0 0 121 145 111 601.0 0 111 146 114 601.0 0 114 147 94 601.0 0 94 148 112 601.0 0 112 149 54 601.0 0 54 150 68 601.0 0 68 151 201 601.0 0 201 152 286 601.0 0 286 153 428 601.0 0 428 154 121 601.0 0 121 155 121 601.0 0 121 156 130 601.0 0 130 157 115 601.0 0 115 158 1294 601.0 0 1294 159 1289 601.0 0 1289 160 1361 601.0 0 1361 161 567 601.0 0 567 162 251 601.0 0 251 163 144 601.0 0 144 164 75 601.0 0 75 165 86 601.0 0 86 166 58 601.0 0 58 167 90 601.0 0 90 168 122 601.0 0 122 169 201 601.0 0 201 170 97 601.0 0 97 171 103 601.0 0 103 172 62 601.0 0 62 173 80 601.0 0 80 174 82 601.0 0 82 175 79 601.0 0 79 176 106 601.0 0 106 177 88 601.0 0 88 178 49 601.0 0 49 179 56 601.0 0 56 180 76 601.0 0 76 181 132 601.0 0 132 182 181 601.0 0 181 183 105 601.0 0 105 184 74 601.0 0 74 185 77 601.0 0 77 186 55 601.0 0 55 187 86 601.0 0 86 188 117 601.0 0 117 189 178 601.0 0 178 190 172 601.0 0 172 191 46 601.0 0 46 192 71 601.0 0 71 193 69 601.0 0 69 194 61 601.0 0 61 195 49 601.0 0 49 196 55 601.0 0 55 197 80 601.0 0 80 198 86 601.0 0 86 199 79 601.0 0 79 200 99 601.0 0 99 201 166 601.0 0 166 202 88 601.0 0 88 203 78 601.0 0 78 204 76 601.0 0 76 205 72 601.0 0 72 206 78 601.0 0 78 207 125 601.0 0 125 208 99 601.0 0 99 209 95 601.0 0 95 210 71 601.0 0 71 211 112 601.0 0 112 212 70 601.0 0 70 213 118 601.0 0 118 214 182 601.0 0 182 215 200 601.0 0 200 216 247 601.0 0 247 217 67 601.0 0 67 218 88 601.0 0 88 219 75 601.0 0 75 220 204 601.0 0 204 221 461 601.0 0 461 222 269 601.0 0 269 223 131 601.0 0 131 224 58 601.0 0 58 225 142 601.0 0 142 226 162 601.0 0 162 227 108 601.0 0 108 228 61 601.0 0 61 229 53 601.0 0 53 230 72 601.0 0 72 231 193 601.0 0 193 232 220 601.0 0 220 233 320 601.0 0 320 234 148 601.0 0 148 235 216 601.0 0 216 236 99 601.0 0 99 237 87 601.0 0 87 238 73 601.0 0 73 239 62 601.0 0 62 240 79 601.0 0 79 241 130 601.0 0 130 242 172 601.0 0 172 243 141 601.0 0 141 244 130 601.0 0 130 245 98 601.0 0 98 246 74 601.0 0 74 247 77 601.0 0 77 248 78 601.0 0 78 249 63 601.0 0 63 250 69 601.0 0 69 251 89 601.0 0 89 252 103 601.0 0 103 253 136 601.0 0 136 254 96 601.0 0 96 255 84 601.0 0 84 256 111 601.0 0 111 257 90 601.0 0 90 258 57 601.0 0 57 259 56 601.0 0 56 260 92 601.0 0 92 261 113 601.0 0 113 262 172 601.0 0 172 263 179 601.0 0 179 264 138 601.0 0 138 265 100 601.0 0 100 266 90 601.0 0 90 267 264 601.0 0 264 268 299 601.0 0 299 269 228 601.0 0 228 270 141 601.0 0 141 271 99 601.0 0 99 272 86 601.0 0 86 273 69 601.0 0 69 274 90 601.0 0 90 275 87 601.0 0 87 276 134 601.0 0 134 277 348 601.0 0 348 278 251 601.0 0 251 279 332 601.0 0 332 280 386 601.0 0 386 281 1566 601.0 0 1566 282 2497 601.0 0 2497 283 3357 601.0 0 3357 284 1071 601.0 0 1071 285 84 601.0 0 84 286 129 601.0 0 129 287 112 601.0 0 112 288 597 601.0 0 597 289 385 601.0 0 385 290 378 601.0 0 378 291 221 601.0 0 221 292 200 601.0 0 200 293 92 601.0 0 92 294 121 601.0 0 121 295 128 601.0 0 128 296 109 601.0 0 109 297 72 601.0 0 72 298 61 601.0 0 61 299 46 601.0 0 46 300 49 601.0 0 49 301 89 601.0 0 89 Finished in 17.22 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 344,818 Reads with adapters: 85,479 (24.8%) Reads that were too short: 209 (0.1%) Reads written (passing filters): 85,270 (24.7%) Total basepairs processed: 97,480,369 bp Total written (filtered): 23,036,223 bp (23.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 85479 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 11 0.0 3 0 0 0 6 5 18 41 0.0 3 0 0 12 17 12 19 354 0.0 3 0 237 41 41 35 20 82749 0.0 4 76368 4884 1083 312 102 21 232 0.0 4 18 188 10 9 7 22 26 0.0 4 2 1 13 3 7 23 315 0.0 4 1 0 0 191 123 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 27 3 0.0 4 2 0 0 0 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 1 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 2 0.0 4 2 36 1 0.0 4 0 0 0 0 1 39 2 0.0 4 1 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 81 2 0.0 4 1 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 87 9 0.0 4 0 0 0 0 9 88 2 0.0 4 1 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 8 0.0 4 0 0 0 0 8 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 104 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 109 5 0.0 4 0 0 0 0 5 110 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 1 119 1 0.0 4 0 0 0 0 1 120 4 0.0 4 0 0 0 0 4 121 10 0.0 4 0 0 0 0 10 122 765 0.0 4 0 0 0 0 765 123 6 0.0 4 0 0 0 0 6 124 3 0.0 4 0 0 0 0 3 125 3 0.0 4 0 0 0 1 2 126 7 0.0 4 0 0 0 0 7 127 666 0.0 4 0 0 0 1 665 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 9 0.0 4 0 0 0 0 9 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 1 140 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 5 0.0 4 0 0 0 0 5 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 154 1 0.0 4 1 181 1 0.0 4 0 0 0 0 1 188 2 0.0 4 0 0 0 0 2 199 1 0.0 4 0 0 0 0 1 201 2 0.0 4 0 0 0 0 2 202 4 0.0 4 0 0 0 0 4 203 3 0.0 4 0 0 0 0 3 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 4 0.0 4 0 0 0 0 4 209 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 213 3 0.0 4 0 0 0 0 3 214 1 0.0 4 0 0 0 0 1 215 5 0.0 4 0 0 0 0 5 216 2 0.0 4 0 0 0 0 2 218 2 0.0 4 0 0 0 0 2 221 1 0.0 4 0 0 0 0 1 222 2 0.0 4 0 0 0 0 2 223 21 0.0 4 0 0 0 1 20 224 15 0.0 4 0 0 0 0 15 225 4 0.0 4 0 0 0 0 4 226 2 0.0 4 0 0 0 0 2 227 3 0.0 4 0 0 0 0 3 228 11 0.0 4 0 0 0 0 11 229 13 0.0 4 0 0 0 0 13 230 3 0.0 4 0 0 0 0 3 231 3 0.0 4 0 0 0 0 3 232 5 0.0 4 0 0 0 0 5 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 1 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 252 5 0.0 4 0 0 0 0 5 254 2 0.0 4 0 0 0 0 2 255 1 0.0 4 0 0 0 0 1 257 5 0.0 4 0 0 0 0 5 258 1 0.0 4 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 4 0.0 4 0 0 0 0 4 263 2 0.0 4 0 0 0 0 2 264 7 0.0 4 0 0 0 1 6 265 4 0.0 4 0 0 0 1 3 268 4 0.0 4 0 0 0 0 4 269 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 277 3 0.0 4 0 0 0 0 3 278 3 0.0 4 0 0 0 0 3 280 1 0.0 4 0 0 0 0 1 281 3 0.0 4 0 0 0 0 3 282 3 0.0 4 0 0 0 0 3 283 4 0.0 4 0 0 0 0 4 286 2 0.0 4 0 0 0 0 2 287 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 293 3 0.0 4 0 0 0 0 3 294 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.14 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 85,270 Reads with adapters: 22,173 (26.0%) Reads written (passing filters): 85,270 (100.0%) Total basepairs processed: 23,036,223 bp Total written (filtered): 22,806,452 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 22173 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 10.9% G: 84.6% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 60 1332.3 0 60 4 22 333.1 0 22 5 6 83.3 0 6 6 3 20.8 0 3 7 4 5.2 0 4 8 9 1.3 0 9 9 9688 0.3 0 1112 8576 10 8925 0.1 1 641 8284 11 3237 0.0 1 120 3117 12 97 0.0 1 1 96 13 27 0.0 1 0 27 14 4 0.0 1 0 4 15 1 0.0 1 0 1 114 1 0.0 1 1 147 2 0.0 1 2 150 42 0.0 1 19 23 151 2 0.0 1 0 2 152 4 0.0 1 0 4 182 1 0.0 1 1 189 3 0.0 1 0 3 205 1 0.0 1 1 208 27 0.0 1 23 4 209 1 0.0 1 1 210 1 0.0 1 0 1 211 1 0.0 1 1 220 1 0.0 1 0 1 226 1 0.0 1 1 228 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.