This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.70 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 350,359 (14.2%) Reads that were too short: 12,544 (0.5%) Reads written (passing filters): 337,815 (13.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 96,403,083 bp (13.0%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 350359 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1583 601.0 0 1583 7 89660 601.0 0 89660 8 1761 601.0 0 1761 9 113416 601.0 0 113416 10 834 601.0 0 834 11 964 601.0 0 964 12 22872 601.0 0 22872 13 78179 601.0 0 78179 14 11996 601.0 0 11996 15 41 601.0 0 41 16 46 601.0 0 46 17 44 601.0 0 44 18 32 601.0 0 32 19 27 601.0 0 27 20 41 601.0 0 41 21 35 601.0 0 35 22 35 601.0 0 35 23 31 601.0 0 31 24 37 601.0 0 37 25 30 601.0 0 30 26 37 601.0 0 37 27 38 601.0 0 38 28 32 601.0 0 32 29 29 601.0 0 29 30 34 601.0 0 34 31 31 601.0 0 31 32 27 601.0 0 27 33 28 601.0 0 28 34 25 601.0 0 25 35 26 601.0 0 26 36 37 601.0 0 37 37 38 601.0 0 38 38 48 601.0 0 48 39 31 601.0 0 31 40 52 601.0 0 52 41 41 601.0 0 41 42 43 601.0 0 43 43 48 601.0 0 48 44 116 601.0 0 116 45 146 601.0 0 146 46 35 601.0 0 35 47 24 601.0 0 24 48 39 601.0 0 39 49 45 601.0 0 45 50 45 601.0 0 45 51 37 601.0 0 37 52 48 601.0 0 48 53 53 601.0 0 53 54 35 601.0 0 35 55 26 601.0 0 26 56 31 601.0 0 31 57 30 601.0 0 30 58 48 601.0 0 48 59 34 601.0 0 34 60 31 601.0 0 31 61 27 601.0 0 27 62 27 601.0 0 27 63 46 601.0 0 46 64 33 601.0 0 33 65 50 601.0 0 50 66 45 601.0 0 45 67 47 601.0 0 47 68 37 601.0 0 37 69 39 601.0 0 39 70 26 601.0 0 26 71 30 601.0 0 30 72 38 601.0 0 38 73 43 601.0 0 43 74 52 601.0 0 52 75 33 601.0 0 33 76 38 601.0 0 38 77 47 601.0 0 47 78 54 601.0 0 54 79 52 601.0 0 52 80 51 601.0 0 51 81 40 601.0 0 40 82 49 601.0 0 49 83 42 601.0 0 42 84 49 601.0 0 49 85 44 601.0 0 44 86 61 601.0 0 61 87 63 601.0 0 63 88 48 601.0 0 48 89 52 601.0 0 52 90 79 601.0 0 79 91 69 601.0 0 69 92 90 601.0 0 90 93 54 601.0 0 54 94 66 601.0 0 66 95 50 601.0 0 50 96 44 601.0 0 44 97 35 601.0 0 35 98 54 601.0 0 54 99 47 601.0 0 47 100 47 601.0 0 47 101 37 601.0 0 37 102 61 601.0 0 61 103 53 601.0 0 53 104 202 601.0 0 202 105 140 601.0 0 140 106 139 601.0 0 139 107 60 601.0 0 60 108 69 601.0 0 69 109 51 601.0 0 51 110 76 601.0 0 76 111 80 601.0 0 80 112 75 601.0 0 75 113 81 601.0 0 81 114 46 601.0 0 46 115 56 601.0 0 56 116 107 601.0 0 107 117 81 601.0 0 81 118 54 601.0 0 54 119 64 601.0 0 64 120 64 601.0 0 64 121 62 601.0 0 62 122 57 601.0 0 57 123 58 601.0 0 58 124 76 601.0 0 76 125 95 601.0 0 95 126 473 601.0 0 473 127 288 601.0 0 288 128 712 601.0 0 712 129 214 601.0 0 214 130 55 601.0 0 55 131 47 601.0 0 47 132 65 601.0 0 65 133 58 601.0 0 58 134 61 601.0 0 61 135 63 601.0 0 63 136 68 601.0 0 68 137 75 601.0 0 75 138 185 601.0 0 185 139 116 601.0 0 116 140 255 601.0 0 255 141 153 601.0 0 153 142 133 601.0 0 133 143 71 601.0 0 71 144 68 601.0 0 68 145 69 601.0 0 69 146 57 601.0 0 57 147 63 601.0 0 63 148 48 601.0 0 48 149 46 601.0 0 46 150 55 601.0 0 55 151 146 601.0 0 146 152 186 601.0 0 186 153 230 601.0 0 230 154 69 601.0 0 69 155 76 601.0 0 76 156 101 601.0 0 101 157 100 601.0 0 100 158 1376 601.0 0 1376 159 1027 601.0 0 1027 160 1247 601.0 0 1247 161 195 601.0 0 195 162 142 601.0 0 142 163 74 601.0 0 74 164 58 601.0 0 58 165 57 601.0 0 57 166 38 601.0 0 38 167 46 601.0 0 46 168 85 601.0 0 85 169 295 601.0 0 295 170 95 601.0 0 95 171 84 601.0 0 84 172 44 601.0 0 44 173 44 601.0 0 44 174 66 601.0 0 66 175 54 601.0 0 54 176 54 601.0 0 54 177 43 601.0 0 43 178 45 601.0 0 45 179 64 601.0 0 64 180 46 601.0 0 46 181 59 601.0 0 59 182 66 601.0 0 66 183 58 601.0 0 58 184 47 601.0 0 47 185 44 601.0 0 44 186 50 601.0 0 50 187 37 601.0 0 37 188 56 601.0 0 56 189 73 601.0 0 73 190 112 601.0 0 112 191 33 601.0 0 33 192 64 601.0 0 64 193 54 601.0 0 54 194 47 601.0 0 47 195 43 601.0 0 43 196 39 601.0 0 39 197 51 601.0 0 51 198 56 601.0 0 56 199 53 601.0 0 53 200 57 601.0 0 57 201 57 601.0 0 57 202 59 601.0 0 59 203 49 601.0 0 49 204 61 601.0 0 61 205 38 601.0 0 38 206 44 601.0 0 44 207 54 601.0 0 54 208 58 601.0 0 58 209 63 601.0 0 63 210 60 601.0 0 60 211 49 601.0 0 49 212 43 601.0 0 43 213 44 601.0 0 44 214 112 601.0 0 112 215 99 601.0 0 99 216 146 601.0 0 146 217 38 601.0 0 38 218 84 601.0 0 84 219 52 601.0 0 52 220 95 601.0 0 95 221 197 601.0 0 197 222 183 601.0 0 183 223 74 601.0 0 74 224 66 601.0 0 66 225 143 601.0 0 143 226 140 601.0 0 140 227 82 601.0 0 82 228 36 601.0 0 36 229 52 601.0 0 52 230 47 601.0 0 47 231 76 601.0 0 76 232 100 601.0 0 100 233 265 601.0 0 265 234 138 601.0 0 138 235 250 601.0 0 250 236 81 601.0 0 81 237 76 601.0 0 76 238 56 601.0 0 56 239 38 601.0 0 38 240 49 601.0 0 49 241 80 601.0 0 80 242 81 601.0 0 81 243 67 601.0 0 67 244 67 601.0 0 67 245 49 601.0 0 49 246 54 601.0 0 54 247 50 601.0 0 50 248 39 601.0 0 39 249 61 601.0 0 61 250 58 601.0 0 58 251 69 601.0 0 69 252 69 601.0 0 69 253 73 601.0 0 73 254 58 601.0 0 58 255 47 601.0 0 47 256 67 601.0 0 67 257 75 601.0 0 75 258 61 601.0 0 61 259 49 601.0 0 49 260 71 601.0 0 71 261 82 601.0 0 82 262 167 601.0 0 167 263 194 601.0 0 194 264 152 601.0 0 152 265 52 601.0 0 52 266 50 601.0 0 50 267 149 601.0 0 149 268 133 601.0 0 133 269 105 601.0 0 105 270 77 601.0 0 77 271 66 601.0 0 66 272 54 601.0 0 54 273 58 601.0 0 58 274 55 601.0 0 55 275 81 601.0 0 81 276 79 601.0 0 79 277 321 601.0 0 321 278 259 601.0 0 259 279 260 601.0 0 260 280 182 601.0 0 182 281 618 601.0 0 618 282 1297 601.0 0 1297 283 1146 601.0 0 1146 284 457 601.0 0 457 285 47 601.0 0 47 286 94 601.0 0 94 287 97 601.0 0 97 288 295 601.0 0 295 289 200 601.0 0 200 290 233 601.0 0 233 291 132 601.0 0 132 292 116 601.0 0 116 293 70 601.0 0 70 294 84 601.0 0 84 295 83 601.0 0 83 296 85 601.0 0 85 297 62 601.0 0 62 298 53 601.0 0 53 299 35 601.0 0 35 300 47 601.0 0 47 301 75 601.0 0 75 Finished in 14.92 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 337,815 Reads with adapters: 89,748 (26.6%) Reads that were too short: 182 (0.1%) Reads written (passing filters): 89,566 (26.5%) Total basepairs processed: 96,403,083 bp Total written (filtered): 24,693,841 bp (25.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 89748 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 6 1 16 278 0.0 3 0 273 5 17 88284 0.0 3 86521 1713 48 2 18 173 0.0 3 10 161 2 19 38 0.0 3 0 0 34 4 20 41 0.0 3 20 0 0 21 22 1 0.0 3 0 0 1 25 1 0.0 3 0 0 0 1 26 2 0.0 3 1 0 0 1 27 1 0.0 3 1 32 16 0.0 3 1 0 0 15 34 1 0.0 3 1 35 3 0.0 3 0 1 0 2 42 2 0.0 3 0 0 0 2 43 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 67 7 0.0 3 0 0 0 7 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 3 105 1 0.0 3 0 0 0 1 110 13 0.0 3 0 0 0 13 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 127 12 0.0 3 1 0 0 11 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 141 1 0.0 3 1 144 1 0.0 3 0 0 0 1 154 24 0.0 3 0 0 0 24 155 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 157 10 0.0 3 0 0 0 10 158 3 0.0 3 0 0 0 3 159 96 0.0 3 0 0 0 96 160 29 0.0 3 0 0 0 29 161 10 0.0 3 0 0 0 10 162 29 0.0 3 0 0 0 29 163 10 0.0 3 0 0 0 10 164 2 0.0 3 0 0 0 2 165 7 0.0 3 0 0 0 7 168 4 0.0 3 3 0 0 1 170 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 177 3 0.0 3 0 0 0 3 178 53 0.0 3 0 0 0 53 179 156 0.0 3 0 0 0 156 180 159 0.0 3 0 0 0 159 181 2 0.0 3 0 0 0 2 182 24 0.0 3 0 0 1 23 183 16 0.0 3 0 0 0 16 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 188 3 0.0 3 0 0 0 3 191 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 8 0.0 3 0 0 0 8 216 1 0.0 3 0 0 0 1 217 17 0.0 3 0 0 0 17 218 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 3 0.0 3 0 0 0 3 232 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 11 0.0 3 0 0 0 11 240 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 2 0.0 3 0 0 0 2 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 271 8 0.0 3 0 0 0 8 272 16 0.0 3 0 0 0 16 273 5 0.0 3 0 0 0 5 274 8 0.0 3 0 0 1 7 275 11 0.0 3 0 0 2 9 276 1 0.0 3 0 0 0 1 277 5 0.0 3 0 0 0 5 279 2 0.0 3 0 0 0 2 280 8 0.0 3 0 0 0 8 281 16 0.0 3 0 0 1 15 282 9 0.0 3 0 0 0 9 283 2 0.0 3 0 0 0 2 284 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.12 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 89,566 Reads with adapters: 408 (0.5%) Reads written (passing filters): 89,566 (100.0%) Total basepairs processed: 24,693,841 bp Total written (filtered): 24,692,556 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 408 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.7% C: 19.6% G: 17.6% T: 48.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 356 1399.5 0 356 4 45 349.9 0 45 5 5 87.5 0 5 6 2 21.9 0 2