This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.22 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 64,705 (2.6%) Reads that were too short: 19,370 (0.8%) Reads written (passing filters): 45,335 (1.8%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,741,201 bp (1.4%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 64705 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2304 601.0 0 2304 7 3820 601.0 0 3820 8 201 601.0 0 201 9 4621 601.0 0 4621 10 409 601.0 0 409 11 11676 601.0 0 11676 12 67 601.0 0 67 13 64 601.0 0 64 14 43 601.0 0 43 15 55 601.0 0 55 16 64 601.0 0 64 17 69 601.0 0 69 18 54 601.0 0 54 19 56 601.0 0 56 20 69 601.0 0 69 21 85 601.0 0 85 22 83 601.0 0 83 23 59 601.0 0 59 24 42 601.0 0 42 25 51 601.0 0 51 26 56 601.0 0 56 27 58 601.0 0 58 28 43 601.0 0 43 29 84 601.0 0 84 30 69 601.0 0 69 31 63 601.0 0 63 32 50 601.0 0 50 33 61 601.0 0 61 34 56 601.0 0 56 35 57 601.0 0 57 36 62 601.0 0 62 37 73 601.0 0 73 38 47 601.0 0 47 39 50 601.0 0 50 40 63 601.0 0 63 41 70 601.0 0 70 42 63 601.0 0 63 43 42 601.0 0 42 44 57 601.0 0 57 45 38 601.0 0 38 46 52 601.0 0 52 47 53 601.0 0 53 48 42 601.0 0 42 49 71 601.0 0 71 50 65 601.0 0 65 51 58 601.0 0 58 52 87 601.0 0 87 53 90 601.0 0 90 54 72 601.0 0 72 55 93 601.0 0 93 56 62 601.0 0 62 57 57 601.0 0 57 58 80 601.0 0 80 59 55 601.0 0 55 60 53 601.0 0 53 61 53 601.0 0 53 62 58 601.0 0 58 63 40 601.0 0 40 64 66 601.0 0 66 65 77 601.0 0 77 66 46 601.0 0 46 67 66 601.0 0 66 68 74 601.0 0 74 69 84 601.0 0 84 70 99 601.0 0 99 71 105 601.0 0 105 72 105 601.0 0 105 73 279 601.0 0 279 74 93 601.0 0 93 75 206 601.0 0 206 76 71 601.0 0 71 77 60 601.0 0 60 78 51 601.0 0 51 79 69 601.0 0 69 80 74 601.0 0 74 81 76 601.0 0 76 82 95 601.0 0 95 83 62 601.0 0 62 84 62 601.0 0 62 85 56 601.0 0 56 86 72 601.0 0 72 87 55 601.0 0 55 88 72 601.0 0 72 89 67 601.0 0 67 90 73 601.0 0 73 91 69 601.0 0 69 92 58 601.0 0 58 93 52 601.0 0 52 94 63 601.0 0 63 95 78 601.0 0 78 96 92 601.0 0 92 97 64 601.0 0 64 98 66 601.0 0 66 99 93 601.0 0 93 100 84 601.0 0 84 101 839 601.0 0 839 102 430 601.0 0 430 103 586 601.0 0 586 104 82 601.0 0 82 105 127 601.0 0 127 106 106 601.0 0 106 107 65 601.0 0 65 108 78 601.0 0 78 109 72 601.0 0 72 110 80 601.0 0 80 111 246 601.0 0 246 112 479 601.0 0 479 113 434 601.0 0 434 114 158 601.0 0 158 115 145 601.0 0 145 116 107 601.0 0 107 117 116 601.0 0 116 118 110 601.0 0 110 119 68 601.0 0 68 120 54 601.0 0 54 121 65 601.0 0 65 122 74 601.0 0 74 123 123 601.0 0 123 124 133 601.0 0 133 125 130 601.0 0 130 126 94 601.0 0 94 127 75 601.0 0 75 128 69 601.0 0 69 129 74 601.0 0 74 130 93 601.0 0 93 131 124 601.0 0 124 132 248 601.0 0 248 133 201 601.0 0 201 134 259 601.0 0 259 135 60 601.0 0 60 136 109 601.0 0 109 137 294 601.0 0 294 138 250 601.0 0 250 139 206 601.0 0 206 140 97 601.0 0 97 141 188 601.0 0 188 142 129 601.0 0 129 143 150 601.0 0 150 144 282 601.0 0 282 145 317 601.0 0 317 146 1002 601.0 0 1002 147 62 601.0 0 62 148 84 601.0 0 84 149 170 601.0 0 170 150 138 601.0 0 138 151 140 601.0 0 140 152 73 601.0 0 73 153 78 601.0 0 78 154 130 601.0 0 130 155 127 601.0 0 127 156 244 601.0 0 244 157 108 601.0 0 108 158 111 601.0 0 111 159 118 601.0 0 118 160 136 601.0 0 136 161 100 601.0 0 100 162 70 601.0 0 70 163 93 601.0 0 93 164 106 601.0 0 106 165 99 601.0 0 99 166 140 601.0 0 140 167 72 601.0 0 72 168 116 601.0 0 116 169 136 601.0 0 136 170 326 601.0 0 326 171 397 601.0 0 397 172 550 601.0 0 550 173 73 601.0 0 73 174 60 601.0 0 60 175 74 601.0 0 74 176 53 601.0 0 53 177 102 601.0 0 102 178 166 601.0 0 166 179 300 601.0 0 300 180 282 601.0 0 282 181 83 601.0 0 83 182 84 601.0 0 84 183 94 601.0 0 94 184 116 601.0 0 116 185 123 601.0 0 123 186 107 601.0 0 107 187 99 601.0 0 99 188 70 601.0 0 70 189 87 601.0 0 87 190 93 601.0 0 93 191 62 601.0 0 62 192 66 601.0 0 66 193 65 601.0 0 65 194 68 601.0 0 68 195 77 601.0 0 77 196 67 601.0 0 67 197 69 601.0 0 69 198 91 601.0 0 91 199 131 601.0 0 131 200 102 601.0 0 102 201 90 601.0 0 90 202 97 601.0 0 97 203 121 601.0 0 121 204 252 601.0 0 252 205 186 601.0 0 186 206 102 601.0 0 102 207 66 601.0 0 66 208 56 601.0 0 56 209 74 601.0 0 74 210 158 601.0 0 158 211 199 601.0 0 199 212 225 601.0 0 225 213 82 601.0 0 82 214 107 601.0 0 107 215 72 601.0 0 72 216 86 601.0 0 86 217 87 601.0 0 87 218 115 601.0 0 115 219 119 601.0 0 119 220 81 601.0 0 81 221 88 601.0 0 88 222 90 601.0 0 90 223 150 601.0 0 150 224 168 601.0 0 168 225 210 601.0 0 210 226 785 601.0 0 785 227 632 601.0 0 632 228 505 601.0 0 505 229 151 601.0 0 151 230 94 601.0 0 94 231 71 601.0 0 71 232 82 601.0 0 82 233 93 601.0 0 93 234 98 601.0 0 98 235 75 601.0 0 75 236 111 601.0 0 111 237 89 601.0 0 89 238 59 601.0 0 59 239 86 601.0 0 86 240 77 601.0 0 77 241 68 601.0 0 68 242 94 601.0 0 94 243 112 601.0 0 112 244 139 601.0 0 139 245 205 601.0 0 205 246 191 601.0 0 191 247 373 601.0 0 373 248 236 601.0 0 236 249 139 601.0 0 139 250 91 601.0 0 91 251 65 601.0 0 65 252 60 601.0 0 60 253 86 601.0 0 86 254 85 601.0 0 85 255 122 601.0 0 122 256 183 601.0 0 183 257 229 601.0 0 229 258 227 601.0 0 227 259 263 601.0 0 263 260 295 601.0 0 295 261 164 601.0 0 164 262 116 601.0 0 116 263 109 601.0 0 109 264 177 601.0 0 177 265 270 601.0 0 270 266 370 601.0 0 370 267 158 601.0 0 158 268 132 601.0 0 132 269 64 601.0 0 64 270 96 601.0 0 96 271 160 601.0 0 160 272 268 601.0 0 268 273 225 601.0 0 225 274 143 601.0 0 143 275 84 601.0 0 84 276 97 601.0 0 97 277 151 601.0 0 151 278 107 601.0 0 107 279 134 601.0 0 134 280 154 601.0 0 154 281 159 601.0 0 159 282 194 601.0 0 194 283 158 601.0 0 158 284 648 601.0 0 648 285 1075 601.0 0 1075 286 1738 601.0 0 1738 287 1050 601.0 0 1050 288 223 601.0 0 223 289 120 601.0 0 120 290 78 601.0 0 78 291 153 601.0 0 153 292 210 601.0 0 210 293 176 601.0 0 176 294 104 601.0 0 104 295 145 601.0 0 145 296 113 601.0 0 113 297 108 601.0 0 108 298 95 601.0 0 95 299 132 601.0 0 132 300 106 601.0 0 106 301 144 601.0 0 144 Finished in 2.24 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 45,335 Reads with adapters: 6,069 (13.4%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 6,038 (13.3%) Total basepairs processed: 10,741,201 bp Total written (filtered): 1,616,546 bp (15.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6069 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 24 0.0 3 0 15 4 3 2 20 3637 0.0 4 3341 232 48 11 5 21 7 0.0 4 0 6 1 22 12 0.0 4 1 5 2 0 4 23 2032 0.0 4 1863 108 36 15 10 24 10 0.0 4 3 6 1 25 1 0.0 4 1 26 6 0.0 4 0 0 0 3 3 33 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 1 107 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 2 0.0 4 0 0 0 0 2 124 11 0.0 4 0 0 0 0 11 125 273 0.0 4 0 0 0 0 273 126 5 0.0 4 0 0 0 0 5 127 13 0.0 4 0 0 0 0 13 133 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 1 198 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 1 209 1 0.0 4 0 0 0 0 1 226 8 0.0 4 0 0 0 0 8 227 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 289 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 6,038 Reads with adapters: 701 (11.6%) Reads written (passing filters): 6,038 (100.0%) Total basepairs processed: 1,616,546 bp Total written (filtered): 1,609,511 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 701 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.1% C: 14.4% G: 79.9% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 24 94.3 0 24 4 8 23.6 0 8 5 1 5.9 0 1 7 1 0.4 0 1 8 1 0.1 0 1 9 255 0.0 0 34 221 10 219 0.0 1 17 202 11 129 0.0 1 3 126 12 59 0.0 1 3 56 13 2 0.0 1 0 2 123 1 0.0 1 0 1 150 1 0.0 1 0 1