This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.84 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 43,425 (1.8%) Reads that were too short: 10,512 (0.4%) Reads written (passing filters): 32,913 (1.3%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,414,620 bp (1.1%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 43425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2885 601.0 0 2885 7 4939 601.0 0 4939 8 135 601.0 0 135 9 3763 601.0 0 3763 10 281 601.0 0 281 11 9675 601.0 0 9675 12 42 601.0 0 42 13 43 601.0 0 43 14 31 601.0 0 31 15 31 601.0 0 31 16 36 601.0 0 36 17 39 601.0 0 39 18 28 601.0 0 28 19 35 601.0 0 35 20 47 601.0 0 47 21 55 601.0 0 55 22 47 601.0 0 47 23 52 601.0 0 52 24 31 601.0 0 31 25 38 601.0 0 38 26 35 601.0 0 35 27 44 601.0 0 44 28 29 601.0 0 29 29 29 601.0 0 29 30 37 601.0 0 37 31 44 601.0 0 44 32 35 601.0 0 35 33 32 601.0 0 32 34 39 601.0 0 39 35 38 601.0 0 38 36 33 601.0 0 33 37 38 601.0 0 38 38 36 601.0 0 36 39 30 601.0 0 30 40 30 601.0 0 30 41 35 601.0 0 35 42 31 601.0 0 31 43 38 601.0 0 38 44 34 601.0 0 34 45 32 601.0 0 32 46 34 601.0 0 34 47 42 601.0 0 42 48 43 601.0 0 43 49 47 601.0 0 47 50 38 601.0 0 38 51 35 601.0 0 35 52 90 601.0 0 90 53 46 601.0 0 46 54 38 601.0 0 38 55 52 601.0 0 52 56 56 601.0 0 56 57 31 601.0 0 31 58 35 601.0 0 35 59 48 601.0 0 48 60 32 601.0 0 32 61 38 601.0 0 38 62 28 601.0 0 28 63 41 601.0 0 41 64 46 601.0 0 46 65 56 601.0 0 56 66 42 601.0 0 42 67 47 601.0 0 47 68 40 601.0 0 40 69 44 601.0 0 44 70 44 601.0 0 44 71 54 601.0 0 54 72 99 601.0 0 99 73 241 601.0 0 241 74 44 601.0 0 44 75 176 601.0 0 176 76 44 601.0 0 44 77 33 601.0 0 33 78 24 601.0 0 24 79 42 601.0 0 42 80 30 601.0 0 30 81 36 601.0 0 36 82 35 601.0 0 35 83 28 601.0 0 28 84 35 601.0 0 35 85 32 601.0 0 32 86 33 601.0 0 33 87 48 601.0 0 48 88 46 601.0 0 46 89 48 601.0 0 48 90 46 601.0 0 46 91 40 601.0 0 40 92 39 601.0 0 39 93 35 601.0 0 35 94 30 601.0 0 30 95 47 601.0 0 47 96 37 601.0 0 37 97 25 601.0 0 25 98 37 601.0 0 37 99 47 601.0 0 47 100 47 601.0 0 47 101 224 601.0 0 224 102 135 601.0 0 135 103 160 601.0 0 160 104 44 601.0 0 44 105 101 601.0 0 101 106 61 601.0 0 61 107 26 601.0 0 26 108 44 601.0 0 44 109 39 601.0 0 39 110 30 601.0 0 30 111 86 601.0 0 86 112 124 601.0 0 124 113 127 601.0 0 127 114 108 601.0 0 108 115 65 601.0 0 65 116 39 601.0 0 39 117 62 601.0 0 62 118 85 601.0 0 85 119 44 601.0 0 44 120 36 601.0 0 36 121 43 601.0 0 43 122 33 601.0 0 33 123 51 601.0 0 51 124 58 601.0 0 58 125 54 601.0 0 54 126 40 601.0 0 40 127 43 601.0 0 43 128 37 601.0 0 37 129 32 601.0 0 32 130 43 601.0 0 43 131 67 601.0 0 67 132 156 601.0 0 156 133 147 601.0 0 147 134 175 601.0 0 175 135 41 601.0 0 41 136 49 601.0 0 49 137 158 601.0 0 158 138 113 601.0 0 113 139 125 601.0 0 125 140 36 601.0 0 36 141 77 601.0 0 77 142 56 601.0 0 56 143 58 601.0 0 58 144 156 601.0 0 156 145 186 601.0 0 186 146 460 601.0 0 460 147 34 601.0 0 34 148 46 601.0 0 46 149 154 601.0 0 154 150 69 601.0 0 69 151 95 601.0 0 95 152 47 601.0 0 47 153 30 601.0 0 30 154 58 601.0 0 58 155 70 601.0 0 70 156 96 601.0 0 96 157 30 601.0 0 30 158 67 601.0 0 67 159 57 601.0 0 57 160 55 601.0 0 55 161 52 601.0 0 52 162 39 601.0 0 39 163 38 601.0 0 38 164 49 601.0 0 49 165 42 601.0 0 42 166 55 601.0 0 55 167 38 601.0 0 38 168 52 601.0 0 52 169 67 601.0 0 67 170 161 601.0 0 161 171 178 601.0 0 178 172 195 601.0 0 195 173 39 601.0 0 39 174 24 601.0 0 24 175 35 601.0 0 35 176 37 601.0 0 37 177 58 601.0 0 58 178 75 601.0 0 75 179 94 601.0 0 94 180 87 601.0 0 87 181 42 601.0 0 42 182 36 601.0 0 36 183 30 601.0 0 30 184 34 601.0 0 34 185 45 601.0 0 45 186 38 601.0 0 38 187 50 601.0 0 50 188 41 601.0 0 41 189 58 601.0 0 58 190 43 601.0 0 43 191 34 601.0 0 34 192 40 601.0 0 40 193 36 601.0 0 36 194 38 601.0 0 38 195 44 601.0 0 44 196 39 601.0 0 39 197 40 601.0 0 40 198 57 601.0 0 57 199 53 601.0 0 53 200 38 601.0 0 38 201 37 601.0 0 37 202 42 601.0 0 42 203 38 601.0 0 38 204 61 601.0 0 61 205 63 601.0 0 63 206 41 601.0 0 41 207 35 601.0 0 35 208 38 601.0 0 38 209 52 601.0 0 52 210 111 601.0 0 111 211 117 601.0 0 117 212 219 601.0 0 219 213 53 601.0 0 53 214 62 601.0 0 62 215 38 601.0 0 38 216 55 601.0 0 55 217 43 601.0 0 43 218 55 601.0 0 55 219 58 601.0 0 58 220 53 601.0 0 53 221 34 601.0 0 34 222 35 601.0 0 35 223 56 601.0 0 56 224 75 601.0 0 75 225 104 601.0 0 104 226 776 601.0 0 776 227 654 601.0 0 654 228 535 601.0 0 535 229 73 601.0 0 73 230 31 601.0 0 31 231 42 601.0 0 42 232 40 601.0 0 40 233 74 601.0 0 74 234 44 601.0 0 44 235 48 601.0 0 48 236 56 601.0 0 56 237 56 601.0 0 56 238 42 601.0 0 42 239 40 601.0 0 40 240 62 601.0 0 62 241 38 601.0 0 38 242 43 601.0 0 43 243 52 601.0 0 52 244 53 601.0 0 53 245 87 601.0 0 87 246 91 601.0 0 91 247 167 601.0 0 167 248 114 601.0 0 114 249 73 601.0 0 73 250 63 601.0 0 63 251 49 601.0 0 49 252 35 601.0 0 35 253 54 601.0 0 54 254 49 601.0 0 49 255 70 601.0 0 70 256 86 601.0 0 86 257 86 601.0 0 86 258 100 601.0 0 100 259 97 601.0 0 97 260 157 601.0 0 157 261 70 601.0 0 70 262 72 601.0 0 72 263 55 601.0 0 55 264 93 601.0 0 93 265 102 601.0 0 102 266 168 601.0 0 168 267 57 601.0 0 57 268 53 601.0 0 53 269 60 601.0 0 60 270 48 601.0 0 48 271 59 601.0 0 59 272 122 601.0 0 122 273 103 601.0 0 103 274 77 601.0 0 77 275 67 601.0 0 67 276 70 601.0 0 70 277 160 601.0 0 160 278 77 601.0 0 77 279 63 601.0 0 63 280 105 601.0 0 105 281 95 601.0 0 95 282 98 601.0 0 98 283 86 601.0 0 86 284 290 601.0 0 290 285 474 601.0 0 474 286 679 601.0 0 679 287 448 601.0 0 448 288 106 601.0 0 106 289 70 601.0 0 70 290 48 601.0 0 48 291 64 601.0 0 64 292 91 601.0 0 91 293 92 601.0 0 92 294 39 601.0 0 39 295 62 601.0 0 62 296 69 601.0 0 69 297 53 601.0 0 53 298 65 601.0 0 65 299 77 601.0 0 77 300 59 601.0 0 59 301 91 601.0 0 91 Finished in 1.50 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 32,913 Reads with adapters: 3,743 (11.4%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 3,720 (11.3%) Total basepairs processed: 8,414,620 bp Total written (filtered): 1,017,254 bp (12.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3743 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 14 0.0 3 0 13 1 17 3656 0.0 3 3568 86 2 18 8 0.0 3 1 7 19 2 0.0 3 1 0 1 47 4 0.0 3 0 0 0 4 51 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 162 7 0.0 3 0 0 0 7 164 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 14 0.0 3 0 0 0 14 183 4 0.0 3 0 0 0 4 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 274 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 3,720 Reads with adapters: 70 (1.9%) Reads written (passing filters): 3,720 (100.0%) Total basepairs processed: 1,017,254 bp Total written (filtered): 1,017,043 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 70 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 80.0% C: 2.9% G: 15.7% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 69 58.1 0 69 4 1 14.5 0 1