This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.32 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 206,041 (8.4%) Reads that were too short: 101,435 (4.1%) Reads written (passing filters): 104,606 (4.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 27,204,836 bp (3.7%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 206041 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 646 601.0 0 646 7 1345 601.0 0 1345 8 37382 601.0 0 37382 9 32481 601.0 0 32481 10 47 601.0 0 47 11 36 601.0 0 36 12 46 601.0 0 46 13 78 601.0 0 78 14 22 601.0 0 22 15 29 601.0 0 29 16 32 601.0 0 32 17 17 601.0 0 17 18 17 601.0 0 17 19 20 601.0 0 20 20 30 601.0 0 30 21 15 601.0 0 15 22 36 601.0 0 36 23 31 601.0 0 31 24 29 601.0 0 29 25 31 601.0 0 31 26 35 601.0 0 35 27 31 601.0 0 31 28 57 601.0 0 57 29 20 601.0 0 20 30 24 601.0 0 24 31 24 601.0 0 24 32 22 601.0 0 22 33 34 601.0 0 34 34 26 601.0 0 26 35 26 601.0 0 26 36 91 601.0 0 91 37 36 601.0 0 36 38 36 601.0 0 36 39 24 601.0 0 24 40 24 601.0 0 24 41 27 601.0 0 27 42 43 601.0 0 43 43 113 601.0 0 113 44 455 601.0 0 455 45 41 601.0 0 41 46 39 601.0 0 39 47 39 601.0 0 39 48 32 601.0 0 32 49 57 601.0 0 57 50 52 601.0 0 52 51 33 601.0 0 33 52 25 601.0 0 25 53 22 601.0 0 22 54 40 601.0 0 40 55 45 601.0 0 45 56 193 601.0 0 193 57 165 601.0 0 165 58 207 601.0 0 207 59 46 601.0 0 46 60 40 601.0 0 40 61 98 601.0 0 98 62 43 601.0 0 43 63 42 601.0 0 42 64 84 601.0 0 84 65 63 601.0 0 63 66 19 601.0 0 19 67 56 601.0 0 56 68 69 601.0 0 69 69 277 601.0 0 277 70 325 601.0 0 325 71 1443 601.0 0 1443 72 77 601.0 0 77 73 64 601.0 0 64 74 59 601.0 0 59 75 78 601.0 0 78 76 143 601.0 0 143 77 60 601.0 0 60 78 87 601.0 0 87 79 73 601.0 0 73 80 76 601.0 0 76 81 87 601.0 0 87 82 81 601.0 0 81 83 75 601.0 0 75 84 57 601.0 0 57 85 83 601.0 0 83 86 112 601.0 0 112 87 151 601.0 0 151 88 194 601.0 0 194 89 3594 601.0 0 3594 90 7113 601.0 0 7113 91 1992 601.0 0 1992 92 51 601.0 0 51 93 42 601.0 0 42 94 31 601.0 0 31 95 38 601.0 0 38 96 36 601.0 0 36 97 192 601.0 0 192 98 148 601.0 0 148 99 79 601.0 0 79 100 96 601.0 0 96 101 62 601.0 0 62 102 103 601.0 0 103 103 42 601.0 0 42 104 33 601.0 0 33 105 46 601.0 0 46 106 35 601.0 0 35 107 58 601.0 0 58 108 59 601.0 0 59 109 42 601.0 0 42 110 78 601.0 0 78 111 75 601.0 0 75 112 45 601.0 0 45 113 37 601.0 0 37 114 20 601.0 0 20 115 19 601.0 0 19 116 21 601.0 0 21 117 44 601.0 0 44 118 30 601.0 0 30 119 40 601.0 0 40 120 39 601.0 0 39 121 41 601.0 0 41 122 35 601.0 0 35 123 35 601.0 0 35 124 37 601.0 0 37 125 50 601.0 0 50 126 37 601.0 0 37 127 46 601.0 0 46 128 44 601.0 0 44 129 57 601.0 0 57 130 37 601.0 0 37 131 47 601.0 0 47 132 58 601.0 0 58 133 97 601.0 0 97 134 81 601.0 0 81 135 33 601.0 0 33 136 38 601.0 0 38 137 166 601.0 0 166 138 168 601.0 0 168 139 482 601.0 0 482 140 35 601.0 0 35 141 43 601.0 0 43 142 47 601.0 0 47 143 38 601.0 0 38 144 42 601.0 0 42 145 31 601.0 0 31 146 24 601.0 0 24 147 36 601.0 0 36 148 214 601.0 0 214 149 94 601.0 0 94 150 134 601.0 0 134 151 44 601.0 0 44 152 44 601.0 0 44 153 60 601.0 0 60 154 37 601.0 0 37 155 63 601.0 0 63 156 41 601.0 0 41 157 129 601.0 0 129 158 71 601.0 0 71 159 83 601.0 0 83 160 94 601.0 0 94 161 63 601.0 0 63 162 52 601.0 0 52 163 43 601.0 0 43 164 45 601.0 0 45 165 48 601.0 0 48 166 31 601.0 0 31 167 42 601.0 0 42 168 233 601.0 0 233 169 164 601.0 0 164 170 347 601.0 0 347 171 36 601.0 0 36 172 41 601.0 0 41 173 69 601.0 0 69 174 81 601.0 0 81 175 189 601.0 0 189 176 1703 601.0 0 1703 177 1220 601.0 0 1220 178 2995 601.0 0 2995 179 78 601.0 0 78 180 34 601.0 0 34 181 22 601.0 0 22 182 90 601.0 0 90 183 97 601.0 0 97 184 59 601.0 0 59 185 44 601.0 0 44 186 63 601.0 0 63 187 40 601.0 0 40 188 43 601.0 0 43 189 97 601.0 0 97 190 33 601.0 0 33 191 28 601.0 0 28 192 34 601.0 0 34 193 43 601.0 0 43 194 47 601.0 0 47 195 40 601.0 0 40 196 55 601.0 0 55 197 82 601.0 0 82 198 57 601.0 0 57 199 44 601.0 0 44 200 35 601.0 0 35 201 36 601.0 0 36 202 111 601.0 0 111 203 154 601.0 0 154 204 106 601.0 0 106 205 99 601.0 0 99 206 55 601.0 0 55 207 113 601.0 0 113 208 100 601.0 0 100 209 186 601.0 0 186 210 310 601.0 0 310 211 234 601.0 0 234 212 94 601.0 0 94 213 84 601.0 0 84 214 79 601.0 0 79 215 91 601.0 0 91 216 103 601.0 0 103 217 110 601.0 0 110 218 117 601.0 0 117 219 338 601.0 0 338 220 2447 601.0 0 2447 221 2307 601.0 0 2307 222 3234 601.0 0 3234 223 165 601.0 0 165 224 51 601.0 0 51 225 79 601.0 0 79 226 180 601.0 0 180 227 55 601.0 0 55 228 42 601.0 0 42 229 30 601.0 0 30 230 167 601.0 0 167 231 126 601.0 0 126 232 202 601.0 0 202 233 82 601.0 0 82 234 70 601.0 0 70 235 88 601.0 0 88 236 51 601.0 0 51 237 102 601.0 0 102 238 76 601.0 0 76 239 106 601.0 0 106 240 138 601.0 0 138 241 122 601.0 0 122 242 117 601.0 0 117 243 132 601.0 0 132 244 336 601.0 0 336 245 197 601.0 0 197 246 132 601.0 0 132 247 71 601.0 0 71 248 66 601.0 0 66 249 86 601.0 0 86 250 93 601.0 0 93 251 82 601.0 0 82 252 51 601.0 0 51 253 125 601.0 0 125 254 89 601.0 0 89 255 204 601.0 0 204 256 149 601.0 0 149 257 254 601.0 0 254 258 57 601.0 0 57 259 106 601.0 0 106 260 109 601.0 0 109 261 101 601.0 0 101 262 338 601.0 0 338 263 389 601.0 0 389 264 302 601.0 0 302 265 226 601.0 0 226 266 202 601.0 0 202 267 123 601.0 0 123 268 509 601.0 0 509 269 1300 601.0 0 1300 270 771 601.0 0 771 271 520 601.0 0 520 272 186 601.0 0 186 273 116 601.0 0 116 274 1103 601.0 0 1103 275 349 601.0 0 349 276 433 601.0 0 433 277 199 601.0 0 199 278 285 601.0 0 285 279 351 601.0 0 351 280 592 601.0 0 592 281 1090 601.0 0 1090 282 2924 601.0 0 2924 283 18778 601.0 0 18778 284 21365 601.0 0 21365 285 27788 601.0 0 27788 286 4656 601.0 0 4656 287 69 601.0 0 69 288 185 601.0 0 185 289 350 601.0 0 350 290 237 601.0 0 237 291 100 601.0 0 100 292 79 601.0 0 79 293 34 601.0 0 34 294 36 601.0 0 36 295 54 601.0 0 54 296 82 601.0 0 82 297 125 601.0 0 125 298 103 601.0 0 103 299 54 601.0 0 54 300 47 601.0 0 47 301 24 601.0 0 24 Finished in 5.34 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 104,606 Reads with adapters: 33,696 (32.2%) Reads that were too short: 66 (0.1%) Reads written (passing filters): 33,630 (32.1%) Total basepairs processed: 27,204,836 bp Total written (filtered): 9,132,875 bp (33.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33696 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 4 2 18 12 0.0 3 0 0 2 6 4 19 166 0.0 3 0 116 20 15 15 20 33092 0.0 4 30512 2008 401 129 42 21 81 0.0 4 8 62 6 0 5 22 16 0.0 4 5 1 4 2 4 23 109 0.0 4 6 5 1 67 30 28 1 0.0 4 1 37 2 0.0 4 1 0 0 0 1 47 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 1 71 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 86 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 125 3 0.0 4 0 0 0 0 3 126 2 0.0 4 0 0 0 0 2 127 127 0.0 4 0 0 0 0 127 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 146 9 0.0 4 0 0 0 0 9 147 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 33,630 Reads with adapters: 8,323 (24.7%) Reads written (passing filters): 33,630 (100.0%) Total basepairs processed: 9,132,875 bp Total written (filtered): 9,043,526 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8323 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 11.1% G: 84.5% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 525.5 0 19 4 3 131.4 0 3 5 1 32.8 0 1 6 1 8.2 0 1 8 13 0.5 0 13 9 2826 0.1 0 296 2530 10 3832 0.0 1 275 3557 11 1541 0.0 1 54 1487 12 28 0.0 1 1 27 13 11 0.0 1 0 11 14 1 0.0 1 0 1 121 2 0.0 1 2 138 1 0.0 1 1 150 27 0.0 1 21 6 177 1 0.0 1 0 1 207 1 0.0 1 1 208 11 0.0 1 9 2 209 1 0.0 1 1 210 1 0.0 1 1 223 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.