This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.54 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 189,112 (7.7%) Reads that were too short: 81,493 (3.3%) Reads written (passing filters): 107,619 (4.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 28,111,125 bp (3.8%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 189112 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 589 601.0 0 589 7 971 601.0 0 971 8 34793 601.0 0 34793 9 40488 601.0 0 40488 10 48 601.0 0 48 11 19 601.0 0 19 12 13 601.0 0 13 13 37 601.0 0 37 14 9 601.0 0 9 15 18 601.0 0 18 16 14 601.0 0 14 17 8 601.0 0 8 18 7 601.0 0 7 19 11 601.0 0 11 20 9 601.0 0 9 21 24 601.0 0 24 22 10 601.0 0 10 23 9 601.0 0 9 24 14 601.0 0 14 25 12 601.0 0 12 26 13 601.0 0 13 27 13 601.0 0 13 28 22 601.0 0 22 29 15 601.0 0 15 30 12 601.0 0 12 31 16 601.0 0 16 32 11 601.0 0 11 33 11 601.0 0 11 34 10 601.0 0 10 35 16 601.0 0 16 36 27 601.0 0 27 37 19 601.0 0 19 38 35 601.0 0 35 39 16 601.0 0 16 40 16 601.0 0 16 41 14 601.0 0 14 42 19 601.0 0 19 43 121 601.0 0 121 44 458 601.0 0 458 45 46 601.0 0 46 46 19 601.0 0 19 47 20 601.0 0 20 48 21 601.0 0 21 49 30 601.0 0 30 50 33 601.0 0 33 51 22 601.0 0 22 52 12 601.0 0 12 53 9 601.0 0 9 54 22 601.0 0 22 55 19 601.0 0 19 56 37 601.0 0 37 57 57 601.0 0 57 58 388 601.0 0 388 59 68 601.0 0 68 60 18 601.0 0 18 61 48 601.0 0 48 62 28 601.0 0 28 63 26 601.0 0 26 64 46 601.0 0 46 65 31 601.0 0 31 66 12 601.0 0 12 67 30 601.0 0 30 68 31 601.0 0 31 69 75 601.0 0 75 70 85 601.0 0 85 71 389 601.0 0 389 72 27 601.0 0 27 73 35 601.0 0 35 74 37 601.0 0 37 75 74 601.0 0 74 76 124 601.0 0 124 77 37 601.0 0 37 78 53 601.0 0 53 79 51 601.0 0 51 80 49 601.0 0 49 81 64 601.0 0 64 82 65 601.0 0 65 83 52 601.0 0 52 84 44 601.0 0 44 85 70 601.0 0 70 86 81 601.0 0 81 87 125 601.0 0 125 88 147 601.0 0 147 89 3945 601.0 0 3945 90 8311 601.0 0 8311 91 2058 601.0 0 2058 92 26 601.0 0 26 93 17 601.0 0 17 94 18 601.0 0 18 95 14 601.0 0 14 96 20 601.0 0 20 97 40 601.0 0 40 98 46 601.0 0 46 99 32 601.0 0 32 100 31 601.0 0 31 101 27 601.0 0 27 102 27 601.0 0 27 103 25 601.0 0 25 104 18 601.0 0 18 105 32 601.0 0 32 106 17 601.0 0 17 107 24 601.0 0 24 108 27 601.0 0 27 109 11 601.0 0 11 110 53 601.0 0 53 111 25 601.0 0 25 112 21 601.0 0 21 113 11 601.0 0 11 114 8 601.0 0 8 115 6 601.0 0 6 116 12 601.0 0 12 117 17 601.0 0 17 118 22 601.0 0 22 119 14 601.0 0 14 120 12 601.0 0 12 121 18 601.0 0 18 122 17 601.0 0 17 123 16 601.0 0 16 124 24 601.0 0 24 125 19 601.0 0 19 126 15 601.0 0 15 127 12 601.0 0 12 128 35 601.0 0 35 129 46 601.0 0 46 130 17 601.0 0 17 131 46 601.0 0 46 132 20 601.0 0 20 133 63 601.0 0 63 134 55 601.0 0 55 135 13 601.0 0 13 136 20 601.0 0 20 137 144 601.0 0 144 138 155 601.0 0 155 139 471 601.0 0 471 140 17 601.0 0 17 141 9 601.0 0 9 142 17 601.0 0 17 143 13 601.0 0 13 144 14 601.0 0 14 145 23 601.0 0 23 146 16 601.0 0 16 147 11 601.0 0 11 148 71 601.0 0 71 149 53 601.0 0 53 150 32 601.0 0 32 151 29 601.0 0 29 152 13 601.0 0 13 153 25 601.0 0 25 154 21 601.0 0 21 155 43 601.0 0 43 156 30 601.0 0 30 157 59 601.0 0 59 158 28 601.0 0 28 159 51 601.0 0 51 160 40 601.0 0 40 161 32 601.0 0 32 162 28 601.0 0 28 163 32 601.0 0 32 164 32 601.0 0 32 165 33 601.0 0 33 166 36 601.0 0 36 167 33 601.0 0 33 168 320 601.0 0 320 169 201 601.0 0 201 170 482 601.0 0 482 171 37 601.0 0 37 172 49 601.0 0 49 173 73 601.0 0 73 174 97 601.0 0 97 175 148 601.0 0 148 176 2358 601.0 0 2358 177 1480 601.0 0 1480 178 4013 601.0 0 4013 179 43 601.0 0 43 180 19 601.0 0 19 181 6 601.0 0 6 182 26 601.0 0 26 183 35 601.0 0 35 184 34 601.0 0 34 185 26 601.0 0 26 186 44 601.0 0 44 187 30 601.0 0 30 188 22 601.0 0 22 189 86 601.0 0 86 190 33 601.0 0 33 191 17 601.0 0 17 192 21 601.0 0 21 193 19 601.0 0 19 194 24 601.0 0 24 195 20 601.0 0 20 196 17 601.0 0 17 197 16 601.0 0 16 198 28 601.0 0 28 199 18 601.0 0 18 200 22 601.0 0 22 201 17 601.0 0 17 202 51 601.0 0 51 203 84 601.0 0 84 204 58 601.0 0 58 205 48 601.0 0 48 206 19 601.0 0 19 207 55 601.0 0 55 208 78 601.0 0 78 209 82 601.0 0 82 210 145 601.0 0 145 211 54 601.0 0 54 212 41 601.0 0 41 213 32 601.0 0 32 214 31 601.0 0 31 215 58 601.0 0 58 216 75 601.0 0 75 217 85 601.0 0 85 218 113 601.0 0 113 219 278 601.0 0 278 220 2781 601.0 0 2781 221 2560 601.0 0 2560 222 2776 601.0 0 2776 223 106 601.0 0 106 224 28 601.0 0 28 225 28 601.0 0 28 226 184 601.0 0 184 227 35 601.0 0 35 228 27 601.0 0 27 229 26 601.0 0 26 230 61 601.0 0 61 231 63 601.0 0 63 232 164 601.0 0 164 233 50 601.0 0 50 234 49 601.0 0 49 235 51 601.0 0 51 236 30 601.0 0 30 237 38 601.0 0 38 238 54 601.0 0 54 239 64 601.0 0 64 240 54 601.0 0 54 241 78 601.0 0 78 242 51 601.0 0 51 243 55 601.0 0 55 244 193 601.0 0 193 245 102 601.0 0 102 246 77 601.0 0 77 247 43 601.0 0 43 248 35 601.0 0 35 249 42 601.0 0 42 250 35 601.0 0 35 251 64 601.0 0 64 252 38 601.0 0 38 253 85 601.0 0 85 254 58 601.0 0 58 255 172 601.0 0 172 256 116 601.0 0 116 257 243 601.0 0 243 258 34 601.0 0 34 259 50 601.0 0 50 260 54 601.0 0 54 261 65 601.0 0 65 262 113 601.0 0 113 263 109 601.0 0 109 264 156 601.0 0 156 265 87 601.0 0 87 266 181 601.0 0 181 267 77 601.0 0 77 268 211 601.0 0 211 269 567 601.0 0 567 270 456 601.0 0 456 271 291 601.0 0 291 272 143 601.0 0 143 273 131 601.0 0 131 274 1802 601.0 0 1802 275 512 601.0 0 512 276 535 601.0 0 535 277 154 601.0 0 154 278 222 601.0 0 222 279 258 601.0 0 258 280 392 601.0 0 392 281 1069 601.0 0 1069 282 2222 601.0 0 2222 283 15096 601.0 0 15096 284 15689 601.0 0 15689 285 23478 601.0 0 23478 286 4363 601.0 0 4363 287 51 601.0 0 51 288 68 601.0 0 68 289 136 601.0 0 136 290 104 601.0 0 104 291 70 601.0 0 70 292 96 601.0 0 96 293 29 601.0 0 29 294 39 601.0 0 39 295 31 601.0 0 31 296 27 601.0 0 27 297 73 601.0 0 73 298 81 601.0 0 81 299 37 601.0 0 37 300 20 601.0 0 20 301 11 601.0 0 11 Finished in 4.94 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 107,619 Reads with adapters: 35,334 (32.8%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 35,303 (32.8%) Total basepairs processed: 28,111,125 bp Total written (filtered): 9,732,964 bp (34.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35334 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 2 16 121 0.0 3 0 118 3 17 35014 0.0 3 34433 562 19 18 68 0.0 3 9 56 2 1 19 8 0.0 3 0 0 7 1 20 16 0.0 3 0 0 0 16 27 1 0.0 3 0 0 1 32 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 1 95 1 0.0 3 0 0 0 1 101 19 0.0 3 0 0 0 19 102 12 0.0 3 0 0 0 12 103 23 0.0 3 0 0 0 23 105 4 0.0 3 0 0 0 4 110 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 203 4 0.0 3 0 0 0 4 204 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 1 211 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.24 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 35,303 Reads with adapters: 653 (1.8%) Reads written (passing filters): 35,303 (100.0%) Total basepairs processed: 9,732,964 bp Total written (filtered): 9,730,886 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 653 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.7% C: 4.6% G: 13.8% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 544 551.6 0 544 4 105 137.9 0 105 5 3 34.5 0 3 11 1 0.0 1 0 1