This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.66 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 110,238 (4.5%) Reads that were too short: 24,391 (1.0%) Reads written (passing filters): 85,847 (3.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 17,090,862 bp (2.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 110238 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 173 601.0 0 173 7 4105 601.0 0 4105 8 3040 601.0 0 3040 9 87 601.0 0 87 10 66 601.0 0 66 11 85 601.0 0 85 12 60 601.0 0 60 13 61 601.0 0 61 14 73 601.0 0 73 15 69 601.0 0 69 16 70 601.0 0 70 17 88 601.0 0 88 18 192 601.0 0 192 19 114 601.0 0 114 20 89 601.0 0 89 21 90 601.0 0 90 22 70 601.0 0 70 23 62 601.0 0 62 24 59 601.0 0 59 25 65 601.0 0 65 26 77 601.0 0 77 27 66 601.0 0 66 28 99 601.0 0 99 29 71 601.0 0 71 30 125 601.0 0 125 31 72 601.0 0 72 32 68 601.0 0 68 33 105 601.0 0 105 34 229 601.0 0 229 35 78 601.0 0 78 36 88 601.0 0 88 37 93 601.0 0 93 38 95 601.0 0 95 39 74 601.0 0 74 40 88 601.0 0 88 41 61 601.0 0 61 42 94 601.0 0 94 43 85 601.0 0 85 44 42 601.0 0 42 45 76 601.0 0 76 46 135 601.0 0 135 47 77 601.0 0 77 48 85 601.0 0 85 49 158 601.0 0 158 50 87 601.0 0 87 51 120 601.0 0 120 52 82 601.0 0 82 53 89 601.0 0 89 54 79 601.0 0 79 55 81 601.0 0 81 56 122 601.0 0 122 57 71 601.0 0 71 58 117 601.0 0 117 59 105 601.0 0 105 60 90 601.0 0 90 61 104 601.0 0 104 62 80 601.0 0 80 63 76 601.0 0 76 64 101 601.0 0 101 65 119 601.0 0 119 66 226 601.0 0 226 67 420 601.0 0 420 68 164 601.0 0 164 69 214 601.0 0 214 70 252 601.0 0 252 71 147 601.0 0 147 72 189 601.0 0 189 73 207 601.0 0 207 74 166 601.0 0 166 75 206 601.0 0 206 76 248 601.0 0 248 77 366 601.0 0 366 78 416 601.0 0 416 79 1047 601.0 0 1047 80 15367 601.0 0 15367 81 11262 601.0 0 11262 82 12506 601.0 0 12506 83 160 601.0 0 160 84 257 601.0 0 257 85 103 601.0 0 103 86 107 601.0 0 107 87 116 601.0 0 116 88 108 601.0 0 108 89 107 601.0 0 107 90 49 601.0 0 49 91 50 601.0 0 50 92 40 601.0 0 40 93 53 601.0 0 53 94 78 601.0 0 78 95 214 601.0 0 214 96 99 601.0 0 99 97 75 601.0 0 75 98 68 601.0 0 68 99 104 601.0 0 104 100 67 601.0 0 67 101 44 601.0 0 44 102 42 601.0 0 42 103 100 601.0 0 100 104 63 601.0 0 63 105 76 601.0 0 76 106 59 601.0 0 59 107 64 601.0 0 64 108 53 601.0 0 53 109 48 601.0 0 48 110 64 601.0 0 64 111 81 601.0 0 81 112 106 601.0 0 106 113 145 601.0 0 145 114 71 601.0 0 71 115 58 601.0 0 58 116 58 601.0 0 58 117 145 601.0 0 145 118 128 601.0 0 128 119 145 601.0 0 145 120 279 601.0 0 279 121 333 601.0 0 333 122 222 601.0 0 222 123 106 601.0 0 106 124 130 601.0 0 130 125 139 601.0 0 139 126 112 601.0 0 112 127 558 601.0 0 558 128 282 601.0 0 282 129 428 601.0 0 428 130 130 601.0 0 130 131 80 601.0 0 80 132 205 601.0 0 205 133 90 601.0 0 90 134 65 601.0 0 65 135 90 601.0 0 90 136 137 601.0 0 137 137 84 601.0 0 84 138 83 601.0 0 83 139 143 601.0 0 143 140 144 601.0 0 144 141 140 601.0 0 140 142 104 601.0 0 104 143 85 601.0 0 85 144 125 601.0 0 125 145 174 601.0 0 174 146 112 601.0 0 112 147 102 601.0 0 102 148 185 601.0 0 185 149 104 601.0 0 104 150 107 601.0 0 107 151 43 601.0 0 43 152 81 601.0 0 81 153 69 601.0 0 69 154 82 601.0 0 82 155 66 601.0 0 66 156 62 601.0 0 62 157 49 601.0 0 49 158 68 601.0 0 68 159 69 601.0 0 69 160 71 601.0 0 71 161 77 601.0 0 77 162 58 601.0 0 58 163 70 601.0 0 70 164 87 601.0 0 87 165 62 601.0 0 62 166 94 601.0 0 94 167 386 601.0 0 386 168 124 601.0 0 124 169 392 601.0 0 392 170 72 601.0 0 72 171 66 601.0 0 66 172 63 601.0 0 63 173 84 601.0 0 84 174 83 601.0 0 83 175 146 601.0 0 146 176 99 601.0 0 99 177 121 601.0 0 121 178 103 601.0 0 103 179 84 601.0 0 84 180 82 601.0 0 82 181 129 601.0 0 129 182 138 601.0 0 138 183 175 601.0 0 175 184 152 601.0 0 152 185 170 601.0 0 170 186 208 601.0 0 208 187 359 601.0 0 359 188 2692 601.0 0 2692 189 6779 601.0 0 6779 190 4775 601.0 0 4775 191 678 601.0 0 678 192 708 601.0 0 708 193 333 601.0 0 333 194 225 601.0 0 225 195 236 601.0 0 236 196 308 601.0 0 308 197 102 601.0 0 102 198 68 601.0 0 68 199 84 601.0 0 84 200 75 601.0 0 75 201 72 601.0 0 72 202 96 601.0 0 96 203 96 601.0 0 96 204 98 601.0 0 98 205 239 601.0 0 239 206 644 601.0 0 644 207 568 601.0 0 568 208 1954 601.0 0 1954 209 137 601.0 0 137 210 125 601.0 0 125 211 408 601.0 0 408 212 576 601.0 0 576 213 482 601.0 0 482 214 182 601.0 0 182 215 73 601.0 0 73 216 151 601.0 0 151 217 106 601.0 0 106 218 83 601.0 0 83 219 95 601.0 0 95 220 83 601.0 0 83 221 340 601.0 0 340 222 244 601.0 0 244 223 271 601.0 0 271 224 121 601.0 0 121 225 83 601.0 0 83 226 86 601.0 0 86 227 154 601.0 0 154 228 107 601.0 0 107 229 180 601.0 0 180 230 178 601.0 0 178 231 97 601.0 0 97 232 114 601.0 0 114 233 133 601.0 0 133 234 126 601.0 0 126 235 115 601.0 0 115 236 91 601.0 0 91 237 128 601.0 0 128 238 120 601.0 0 120 239 113 601.0 0 113 240 106 601.0 0 106 241 180 601.0 0 180 242 118 601.0 0 118 243 123 601.0 0 123 244 107 601.0 0 107 245 99 601.0 0 99 246 124 601.0 0 124 247 133 601.0 0 133 248 137 601.0 0 137 249 72 601.0 0 72 250 88 601.0 0 88 251 126 601.0 0 126 252 166 601.0 0 166 253 105 601.0 0 105 254 125 601.0 0 125 255 167 601.0 0 167 256 198 601.0 0 198 257 173 601.0 0 173 258 201 601.0 0 201 259 142 601.0 0 142 260 125 601.0 0 125 261 105 601.0 0 105 262 86 601.0 0 86 263 117 601.0 0 117 264 98 601.0 0 98 265 83 601.0 0 83 266 116 601.0 0 116 267 146 601.0 0 146 268 493 601.0 0 493 269 388 601.0 0 388 270 319 601.0 0 319 271 1743 601.0 0 1743 272 1858 601.0 0 1858 273 956 601.0 0 956 274 323 601.0 0 323 275 81 601.0 0 81 276 79 601.0 0 79 277 87 601.0 0 87 278 182 601.0 0 182 279 109 601.0 0 109 280 137 601.0 0 137 281 114 601.0 0 114 282 951 601.0 0 951 283 493 601.0 0 493 284 281 601.0 0 281 285 80 601.0 0 80 286 93 601.0 0 93 287 119 601.0 0 119 288 161 601.0 0 161 289 115 601.0 0 115 290 106 601.0 0 106 291 136 601.0 0 136 292 234 601.0 0 234 293 276 601.0 0 276 294 420 601.0 0 420 295 224 601.0 0 224 296 135 601.0 0 135 297 153 601.0 0 153 298 142 601.0 0 142 299 131 601.0 0 131 300 344 601.0 0 344 301 365 601.0 0 365 Finished in 3.64 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 85,847 Reads with adapters: 3,372 (3.9%) Reads that were too short: 213 (0.2%) Reads written (passing filters): 3,159 (3.7%) Total basepairs processed: 17,090,862 bp Total written (filtered): 857,640 bp (5.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3372 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 12 0.0 3 0 0 0 0 12 18 3 0.0 3 0 0 1 0 2 19 57 0.0 3 0 39 9 5 4 20 3003 0.0 4 2725 205 53 14 6 21 9 0.0 4 1 7 0 0 1 22 26 0.0 4 7 8 7 2 2 23 19 0.0 4 0 0 0 10 9 25 1 0.0 4 0 1 29 2 0.0 4 0 0 0 0 2 30 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 0 2 55 8 0.0 4 0 0 0 0 8 57 3 0.0 4 0 0 0 0 3 58 3 0.0 4 0 0 0 0 3 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 1 0 0 1 97 1 0.0 4 0 0 0 1 104 1 0.0 4 0 0 0 0 1 110 3 0.0 4 0 0 0 0 3 116 2 0.0 4 0 0 0 0 2 127 1 0.0 4 0 0 0 0 1 129 7 0.0 4 0 0 0 0 7 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 4 0.0 4 0 0 0 0 4 155 98 0.0 4 0 0 0 4 94 156 41 0.0 4 0 0 0 3 38 157 11 0.0 4 0 0 0 1 10 159 1 0.0 4 0 0 0 0 1 162 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 3 0.0 4 0 0 0 0 3 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 210 4 0.0 4 0 0 0 0 4 220 1 0.0 4 0 0 0 1 225 4 0.0 4 0 0 0 0 4 228 3 0.0 4 0 0 0 1 2 250 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 3,159 Reads with adapters: 691 (21.9%) Reads written (passing filters): 3,159 (100.0%) Total basepairs processed: 857,640 bp Total written (filtered): 850,034 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 691 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 8.0% G: 88.6% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 49.4 0 2 4 2 12.3 0 2 8 1 0.0 0 1 9 10 0.0 0 1 9 10 48 0.0 1 3 45 11 618 0.0 1 27 591 12 8 0.0 1 0 8 14 1 0.0 1 0 1 106 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.