This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.84 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 94,678 (3.8%) Reads that were too short: 16,506 (0.7%) Reads written (passing filters): 78,172 (3.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 15,637,514 bp (2.1%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 94678 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 97 601.0 0 97 7 3294 601.0 0 3294 8 4115 601.0 0 4115 9 54 601.0 0 54 10 51 601.0 0 51 11 51 601.0 0 51 12 35 601.0 0 35 13 36 601.0 0 36 14 46 601.0 0 46 15 48 601.0 0 48 16 48 601.0 0 48 17 48 601.0 0 48 18 67 601.0 0 67 19 60 601.0 0 60 20 58 601.0 0 58 21 53 601.0 0 53 22 51 601.0 0 51 23 53 601.0 0 53 24 48 601.0 0 48 25 38 601.0 0 38 26 50 601.0 0 50 27 76 601.0 0 76 28 82 601.0 0 82 29 51 601.0 0 51 30 132 601.0 0 132 31 48 601.0 0 48 32 57 601.0 0 57 33 71 601.0 0 71 34 227 601.0 0 227 35 43 601.0 0 43 36 75 601.0 0 75 37 86 601.0 0 86 38 55 601.0 0 55 39 58 601.0 0 58 40 84 601.0 0 84 41 55 601.0 0 55 42 71 601.0 0 71 43 76 601.0 0 76 44 43 601.0 0 43 45 74 601.0 0 74 46 122 601.0 0 122 47 54 601.0 0 54 48 72 601.0 0 72 49 152 601.0 0 152 50 70 601.0 0 70 51 125 601.0 0 125 52 73 601.0 0 73 53 68 601.0 0 68 54 64 601.0 0 64 55 59 601.0 0 59 56 99 601.0 0 99 57 46 601.0 0 46 58 75 601.0 0 75 59 71 601.0 0 71 60 85 601.0 0 85 61 79 601.0 0 79 62 96 601.0 0 96 63 82 601.0 0 82 64 106 601.0 0 106 65 111 601.0 0 111 66 240 601.0 0 240 67 442 601.0 0 442 68 149 601.0 0 149 69 235 601.0 0 235 70 204 601.0 0 204 71 109 601.0 0 109 72 165 601.0 0 165 73 159 601.0 0 159 74 177 601.0 0 177 75 159 601.0 0 159 76 160 601.0 0 160 77 282 601.0 0 282 78 315 601.0 0 315 79 997 601.0 0 997 80 14944 601.0 0 14944 81 10632 601.0 0 10632 82 11806 601.0 0 11806 83 98 601.0 0 98 84 139 601.0 0 139 85 36 601.0 0 36 86 59 601.0 0 59 87 104 601.0 0 104 88 61 601.0 0 61 89 86 601.0 0 86 90 30 601.0 0 30 91 35 601.0 0 35 92 41 601.0 0 41 93 37 601.0 0 37 94 40 601.0 0 40 95 77 601.0 0 77 96 49 601.0 0 49 97 56 601.0 0 56 98 43 601.0 0 43 99 87 601.0 0 87 100 43 601.0 0 43 101 35 601.0 0 35 102 37 601.0 0 37 103 75 601.0 0 75 104 60 601.0 0 60 105 51 601.0 0 51 106 54 601.0 0 54 107 65 601.0 0 65 108 39 601.0 0 39 109 40 601.0 0 40 110 45 601.0 0 45 111 58 601.0 0 58 112 93 601.0 0 93 113 109 601.0 0 109 114 44 601.0 0 44 115 35 601.0 0 35 116 47 601.0 0 47 117 55 601.0 0 55 118 55 601.0 0 55 119 75 601.0 0 75 120 114 601.0 0 114 121 147 601.0 0 147 122 138 601.0 0 138 123 62 601.0 0 62 124 104 601.0 0 104 125 61 601.0 0 61 126 70 601.0 0 70 127 456 601.0 0 456 128 230 601.0 0 230 129 308 601.0 0 308 130 104 601.0 0 104 131 72 601.0 0 72 132 152 601.0 0 152 133 64 601.0 0 64 134 57 601.0 0 57 135 64 601.0 0 64 136 114 601.0 0 114 137 44 601.0 0 44 138 49 601.0 0 49 139 77 601.0 0 77 140 85 601.0 0 85 141 91 601.0 0 91 142 55 601.0 0 55 143 54 601.0 0 54 144 101 601.0 0 101 145 139 601.0 0 139 146 101 601.0 0 101 147 64 601.0 0 64 148 127 601.0 0 127 149 56 601.0 0 56 150 101 601.0 0 101 151 49 601.0 0 49 152 62 601.0 0 62 153 58 601.0 0 58 154 63 601.0 0 63 155 55 601.0 0 55 156 55 601.0 0 55 157 39 601.0 0 39 158 49 601.0 0 49 159 38 601.0 0 38 160 46 601.0 0 46 161 38 601.0 0 38 162 46 601.0 0 46 163 60 601.0 0 60 164 66 601.0 0 66 165 71 601.0 0 71 166 54 601.0 0 54 167 376 601.0 0 376 168 72 601.0 0 72 169 406 601.0 0 406 170 69 601.0 0 69 171 44 601.0 0 44 172 47 601.0 0 47 173 60 601.0 0 60 174 66 601.0 0 66 175 120 601.0 0 120 176 67 601.0 0 67 177 74 601.0 0 74 178 79 601.0 0 79 179 75 601.0 0 75 180 50 601.0 0 50 181 96 601.0 0 96 182 109 601.0 0 109 183 94 601.0 0 94 184 122 601.0 0 122 185 116 601.0 0 116 186 140 601.0 0 140 187 271 601.0 0 271 188 2621 601.0 0 2621 189 7077 601.0 0 7077 190 4911 601.0 0 4911 191 490 601.0 0 490 192 465 601.0 0 465 193 133 601.0 0 133 194 96 601.0 0 96 195 140 601.0 0 140 196 152 601.0 0 152 197 72 601.0 0 72 198 48 601.0 0 48 199 55 601.0 0 55 200 44 601.0 0 44 201 63 601.0 0 63 202 43 601.0 0 43 203 51 601.0 0 51 204 69 601.0 0 69 205 182 601.0 0 182 206 546 601.0 0 546 207 439 601.0 0 439 208 1688 601.0 0 1688 209 63 601.0 0 63 210 79 601.0 0 79 211 146 601.0 0 146 212 182 601.0 0 182 213 187 601.0 0 187 214 123 601.0 0 123 215 65 601.0 0 65 216 165 601.0 0 165 217 113 601.0 0 113 218 84 601.0 0 84 219 98 601.0 0 98 220 51 601.0 0 51 221 373 601.0 0 373 222 199 601.0 0 199 223 290 601.0 0 290 224 105 601.0 0 105 225 65 601.0 0 65 226 50 601.0 0 50 227 119 601.0 0 119 228 53 601.0 0 53 229 70 601.0 0 70 230 69 601.0 0 69 231 73 601.0 0 73 232 49 601.0 0 49 233 46 601.0 0 46 234 50 601.0 0 50 235 52 601.0 0 52 236 43 601.0 0 43 237 59 601.0 0 59 238 42 601.0 0 42 239 76 601.0 0 76 240 44 601.0 0 44 241 68 601.0 0 68 242 56 601.0 0 56 243 76 601.0 0 76 244 73 601.0 0 73 245 56 601.0 0 56 246 46 601.0 0 46 247 75 601.0 0 75 248 87 601.0 0 87 249 60 601.0 0 60 250 55 601.0 0 55 251 73 601.0 0 73 252 87 601.0 0 87 253 56 601.0 0 56 254 84 601.0 0 84 255 88 601.0 0 88 256 95 601.0 0 95 257 82 601.0 0 82 258 111 601.0 0 111 259 84 601.0 0 84 260 68 601.0 0 68 261 85 601.0 0 85 262 64 601.0 0 64 263 78 601.0 0 78 264 57 601.0 0 57 265 50 601.0 0 50 266 58 601.0 0 58 267 87 601.0 0 87 268 197 601.0 0 197 269 170 601.0 0 170 270 183 601.0 0 183 271 1578 601.0 0 1578 272 1736 601.0 0 1736 273 538 601.0 0 538 274 204 601.0 0 204 275 69 601.0 0 69 276 47 601.0 0 47 277 56 601.0 0 56 278 73 601.0 0 73 279 73 601.0 0 73 280 84 601.0 0 84 281 72 601.0 0 72 282 582 601.0 0 582 283 318 601.0 0 318 284 213 601.0 0 213 285 60 601.0 0 60 286 83 601.0 0 83 287 74 601.0 0 74 288 74 601.0 0 74 289 51 601.0 0 51 290 55 601.0 0 55 291 81 601.0 0 81 292 204 601.0 0 204 293 278 601.0 0 278 294 336 601.0 0 336 295 144 601.0 0 144 296 77 601.0 0 77 297 91 601.0 0 91 298 87 601.0 0 87 299 59 601.0 0 59 300 151 601.0 0 151 301 148 601.0 0 148 Finished in 2.95 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 78,172 Reads with adapters: 3,392 (4.3%) Reads that were too short: 45 (0.1%) Reads written (passing filters): 3,347 (4.3%) Total basepairs processed: 15,637,514 bp Total written (filtered): 903,204 bp (5.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3392 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 18 0.0 3 0 17 1 17 3164 0.0 3 3113 51 18 6 0.0 3 0 6 19 4 0.0 3 1 0 2 1 20 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 28 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 16 0.0 3 15 1 57 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 2 0.0 3 1 0 0 1 81 1 0.0 3 0 0 0 1 86 17 0.0 3 0 1 0 16 87 4 0.0 3 0 0 0 4 89 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 91 6 0.0 3 1 0 0 5 92 11 0.0 3 0 0 0 11 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 5 0.0 3 0 0 0 5 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 55 0.0 3 0 0 0 55 112 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 1 1 174 1 0.0 3 0 0 0 1 188 2 0.0 3 0 1 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 7 0.0 3 0 0 0 7 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,347 Reads with adapters: 5 (0.1%) Reads written (passing filters): 3,347 (100.0%) Total basepairs processed: 903,204 bp Total written (filtered): 903,187 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 40.0% G: 20.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 52.3 0 3 4 2 13.1 0 2