This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.99 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 132,993 (5.4%) Reads that were too short: 43,682 (1.8%) Reads written (passing filters): 89,311 (3.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 16,078,039 bp (2.2%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 132993 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 265 601.0 0 265 7 5315 601.0 0 5315 8 144 601.0 0 144 9 111 601.0 0 111 10 143 601.0 0 143 11 211 601.0 0 211 12 138 601.0 0 138 13 167 601.0 0 167 14 128 601.0 0 128 15 102 601.0 0 102 16 138 601.0 0 138 17 103 601.0 0 103 18 97 601.0 0 97 19 121 601.0 0 121 20 101 601.0 0 101 21 96 601.0 0 96 22 116 601.0 0 116 23 133 601.0 0 133 24 119 601.0 0 119 25 146 601.0 0 146 26 126 601.0 0 126 27 123 601.0 0 123 28 134 601.0 0 134 29 91 601.0 0 91 30 100 601.0 0 100 31 118 601.0 0 118 32 110 601.0 0 110 33 115 601.0 0 115 34 102 601.0 0 102 35 122 601.0 0 122 36 128 601.0 0 128 37 98 601.0 0 98 38 107 601.0 0 107 39 98 601.0 0 98 40 100 601.0 0 100 41 106 601.0 0 106 42 120 601.0 0 120 43 163 601.0 0 163 44 132 601.0 0 132 45 118 601.0 0 118 46 130 601.0 0 130 47 112 601.0 0 112 48 112 601.0 0 112 49 103 601.0 0 103 50 114 601.0 0 114 51 83 601.0 0 83 52 93 601.0 0 93 53 83 601.0 0 83 54 116 601.0 0 116 55 119 601.0 0 119 56 128 601.0 0 128 57 161 601.0 0 161 58 122 601.0 0 122 59 98 601.0 0 98 60 115 601.0 0 115 61 121 601.0 0 121 62 107 601.0 0 107 63 95 601.0 0 95 64 113 601.0 0 113 65 104 601.0 0 104 66 124 601.0 0 124 67 143 601.0 0 143 68 100 601.0 0 100 69 125 601.0 0 125 70 151 601.0 0 151 71 127 601.0 0 127 72 146 601.0 0 146 73 261 601.0 0 261 74 1466 601.0 0 1466 75 653 601.0 0 653 76 1052 601.0 0 1052 77 403 601.0 0 403 78 416 601.0 0 416 79 107 601.0 0 107 80 109 601.0 0 109 81 119 601.0 0 119 82 92 601.0 0 92 83 99 601.0 0 99 84 111 601.0 0 111 85 94 601.0 0 94 86 82 601.0 0 82 87 99 601.0 0 99 88 109 601.0 0 109 89 126 601.0 0 126 90 81 601.0 0 81 91 120 601.0 0 120 92 85 601.0 0 85 93 106 601.0 0 106 94 98 601.0 0 98 95 104 601.0 0 104 96 100 601.0 0 100 97 127 601.0 0 127 98 113 601.0 0 113 99 117 601.0 0 117 100 111 601.0 0 111 101 120 601.0 0 120 102 102 601.0 0 102 103 121 601.0 0 121 104 129 601.0 0 129 105 129 601.0 0 129 106 154 601.0 0 154 107 316 601.0 0 316 108 345 601.0 0 345 109 374 601.0 0 374 110 170 601.0 0 170 111 130 601.0 0 130 112 136 601.0 0 136 113 199 601.0 0 199 114 170 601.0 0 170 115 122 601.0 0 122 116 151 601.0 0 151 117 146 601.0 0 146 118 181 601.0 0 181 119 171 601.0 0 171 120 288 601.0 0 288 121 314 601.0 0 314 122 183 601.0 0 183 123 240 601.0 0 240 124 263 601.0 0 263 125 412 601.0 0 412 126 211 601.0 0 211 127 279 601.0 0 279 128 236 601.0 0 236 129 279 601.0 0 279 130 315 601.0 0 315 131 461 601.0 0 461 132 482 601.0 0 482 133 3483 601.0 0 3483 134 17615 601.0 0 17615 135 7569 601.0 0 7569 136 2511 601.0 0 2511 137 400 601.0 0 400 138 238 601.0 0 238 139 230 601.0 0 230 140 1603 601.0 0 1603 141 1815 601.0 0 1815 142 1113 601.0 0 1113 143 105 601.0 0 105 144 174 601.0 0 174 145 144 601.0 0 144 146 160 601.0 0 160 147 305 601.0 0 305 148 262 601.0 0 262 149 566 601.0 0 566 150 158 601.0 0 158 151 173 601.0 0 173 152 204 601.0 0 204 153 286 601.0 0 286 154 1490 601.0 0 1490 155 658 601.0 0 658 156 685 601.0 0 685 157 4366 601.0 0 4366 158 4005 601.0 0 4005 159 5685 601.0 0 5685 160 235 601.0 0 235 161 176 601.0 0 176 162 208 601.0 0 208 163 255 601.0 0 255 164 436 601.0 0 436 165 220 601.0 0 220 166 220 601.0 0 220 167 157 601.0 0 157 168 136 601.0 0 136 169 164 601.0 0 164 170 89 601.0 0 89 171 105 601.0 0 105 172 137 601.0 0 137 173 122 601.0 0 122 174 171 601.0 0 171 175 125 601.0 0 125 176 102 601.0 0 102 177 92 601.0 0 92 178 99 601.0 0 99 179 100 601.0 0 100 180 143 601.0 0 143 181 125 601.0 0 125 182 147 601.0 0 147 183 140 601.0 0 140 184 137 601.0 0 137 185 122 601.0 0 122 186 161 601.0 0 161 187 139 601.0 0 139 188 123 601.0 0 123 189 101 601.0 0 101 190 135 601.0 0 135 191 178 601.0 0 178 192 146 601.0 0 146 193 111 601.0 0 111 194 100 601.0 0 100 195 103 601.0 0 103 196 108 601.0 0 108 197 107 601.0 0 107 198 83 601.0 0 83 199 87 601.0 0 87 200 117 601.0 0 117 201 107 601.0 0 107 202 127 601.0 0 127 203 121 601.0 0 121 204 95 601.0 0 95 205 121 601.0 0 121 206 159 601.0 0 159 207 151 601.0 0 151 208 118 601.0 0 118 209 121 601.0 0 121 210 115 601.0 0 115 211 102 601.0 0 102 212 96 601.0 0 96 213 140 601.0 0 140 214 169 601.0 0 169 215 224 601.0 0 224 216 162 601.0 0 162 217 185 601.0 0 185 218 138 601.0 0 138 219 119 601.0 0 119 220 154 601.0 0 154 221 158 601.0 0 158 222 164 601.0 0 164 223 117 601.0 0 117 224 129 601.0 0 129 225 127 601.0 0 127 226 218 601.0 0 218 227 139 601.0 0 139 228 131 601.0 0 131 229 112 601.0 0 112 230 142 601.0 0 142 231 138 601.0 0 138 232 209 601.0 0 209 233 186 601.0 0 186 234 227 601.0 0 227 235 157 601.0 0 157 236 119 601.0 0 119 237 143 601.0 0 143 238 112 601.0 0 112 239 133 601.0 0 133 240 121 601.0 0 121 241 130 601.0 0 130 242 135 601.0 0 135 243 159 601.0 0 159 244 138 601.0 0 138 245 160 601.0 0 160 246 128 601.0 0 128 247 104 601.0 0 104 248 126 601.0 0 126 249 108 601.0 0 108 250 134 601.0 0 134 251 189 601.0 0 189 252 371 601.0 0 371 253 466 601.0 0 466 254 597 601.0 0 597 255 152 601.0 0 152 256 145 601.0 0 145 257 146 601.0 0 146 258 143 601.0 0 143 259 138 601.0 0 138 260 127 601.0 0 127 261 119 601.0 0 119 262 185 601.0 0 185 263 156 601.0 0 156 264 134 601.0 0 134 265 145 601.0 0 145 266 123 601.0 0 123 267 154 601.0 0 154 268 362 601.0 0 362 269 675 601.0 0 675 270 888 601.0 0 888 271 515 601.0 0 515 272 520 601.0 0 520 273 163 601.0 0 163 274 187 601.0 0 187 275 293 601.0 0 293 276 848 601.0 0 848 277 1345 601.0 0 1345 278 2731 601.0 0 2731 279 1376 601.0 0 1376 280 264 601.0 0 264 281 235 601.0 0 235 282 302 601.0 0 302 283 255 601.0 0 255 284 216 601.0 0 216 285 210 601.0 0 210 286 145 601.0 0 145 287 138 601.0 0 138 288 187 601.0 0 187 289 358 601.0 0 358 290 360 601.0 0 360 291 286 601.0 0 286 292 323 601.0 0 323 293 353 601.0 0 353 294 594 601.0 0 594 295 11381 601.0 0 11381 296 5053 601.0 0 5053 297 2177 601.0 0 2177 298 129 601.0 0 129 299 111 601.0 0 111 300 152 601.0 0 152 301 139 601.0 0 139 Finished in 3.51 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 89,311 Reads with adapters: 2,360 (2.6%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 2,319 (2.6%) Total basepairs processed: 16,078,039 bp Total written (filtered): 633,092 bp (3.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2360 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 22 0.0 3 0 18 2 2 20 2254 0.0 4 1885 304 44 15 6 21 11 0.0 4 3 5 2 0 1 22 1 0.0 4 0 1 23 10 0.0 4 0 0 0 9 1 32 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 81 9 0.0 4 0 0 0 0 9 82 3 0.0 4 0 0 0 1 2 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 5 0.0 4 0 0 0 0 5 103 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 127 14 0.0 4 0 0 0 0 14 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 1 206 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 2,319 Reads with adapters: 247 (10.7%) Reads written (passing filters): 2,319 (100.0%) Total basepairs processed: 633,092 bp Total written (filtered): 630,182 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 247 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 15.0% G: 78.5% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10 36.2 0 10 4 4 9.1 0 4 9 2 0.0 0 0 2 10 1 0.0 1 0 1 11 207 0.0 1 9 198 12 19 0.0 1 2 17 13 1 0.0 1 0 1 14 1 0.0 1 0 1 152 2 0.0 1 1 1