This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.47 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 154,550 (6.3%) Reads that were too short: 56,815 (2.3%) Reads written (passing filters): 97,735 (4.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 17,626,228 bp (2.4%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 154550 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 252 601.0 0 252 7 5512 601.0 0 5512 8 148 601.0 0 148 9 149 601.0 0 149 10 188 601.0 0 188 11 164 601.0 0 164 12 157 601.0 0 157 13 188 601.0 0 188 14 187 601.0 0 187 15 144 601.0 0 144 16 153 601.0 0 153 17 149 601.0 0 149 18 139 601.0 0 139 19 162 601.0 0 162 20 134 601.0 0 134 21 161 601.0 0 161 22 154 601.0 0 154 23 181 601.0 0 181 24 163 601.0 0 163 25 151 601.0 0 151 26 149 601.0 0 149 27 164 601.0 0 164 28 162 601.0 0 162 29 168 601.0 0 168 30 121 601.0 0 121 31 153 601.0 0 153 32 131 601.0 0 131 33 138 601.0 0 138 34 157 601.0 0 157 35 161 601.0 0 161 36 158 601.0 0 158 37 134 601.0 0 134 38 162 601.0 0 162 39 144 601.0 0 144 40 157 601.0 0 157 41 136 601.0 0 136 42 153 601.0 0 153 43 158 601.0 0 158 44 133 601.0 0 133 45 135 601.0 0 135 46 126 601.0 0 126 47 157 601.0 0 157 48 147 601.0 0 147 49 121 601.0 0 121 50 181 601.0 0 181 51 138 601.0 0 138 52 112 601.0 0 112 53 138 601.0 0 138 54 153 601.0 0 153 55 136 601.0 0 136 56 162 601.0 0 162 57 146 601.0 0 146 58 129 601.0 0 129 59 125 601.0 0 125 60 186 601.0 0 186 61 142 601.0 0 142 62 153 601.0 0 153 63 160 601.0 0 160 64 168 601.0 0 168 65 149 601.0 0 149 66 133 601.0 0 133 67 181 601.0 0 181 68 152 601.0 0 152 69 194 601.0 0 194 70 185 601.0 0 185 71 139 601.0 0 139 72 167 601.0 0 167 73 270 601.0 0 270 74 1459 601.0 0 1459 75 644 601.0 0 644 76 953 601.0 0 953 77 193 601.0 0 193 78 234 601.0 0 234 79 178 601.0 0 178 80 172 601.0 0 172 81 193 601.0 0 193 82 150 601.0 0 150 83 141 601.0 0 141 84 151 601.0 0 151 85 144 601.0 0 144 86 131 601.0 0 131 87 175 601.0 0 175 88 162 601.0 0 162 89 184 601.0 0 184 90 156 601.0 0 156 91 181 601.0 0 181 92 124 601.0 0 124 93 146 601.0 0 146 94 103 601.0 0 103 95 152 601.0 0 152 96 146 601.0 0 146 97 160 601.0 0 160 98 156 601.0 0 156 99 191 601.0 0 191 100 171 601.0 0 171 101 168 601.0 0 168 102 133 601.0 0 133 103 206 601.0 0 206 104 152 601.0 0 152 105 166 601.0 0 166 106 189 601.0 0 189 107 186 601.0 0 186 108 218 601.0 0 218 109 341 601.0 0 341 110 198 601.0 0 198 111 156 601.0 0 156 112 187 601.0 0 187 113 175 601.0 0 175 114 222 601.0 0 222 115 152 601.0 0 152 116 170 601.0 0 170 117 242 601.0 0 242 118 219 601.0 0 219 119 206 601.0 0 206 120 362 601.0 0 362 121 417 601.0 0 417 122 222 601.0 0 222 123 268 601.0 0 268 124 270 601.0 0 270 125 301 601.0 0 301 126 262 601.0 0 262 127 289 601.0 0 289 128 237 601.0 0 237 129 278 601.0 0 278 130 327 601.0 0 327 131 469 601.0 0 469 132 536 601.0 0 536 133 3467 601.0 0 3467 134 17545 601.0 0 17545 135 8033 601.0 0 8033 136 2607 601.0 0 2607 137 344 601.0 0 344 138 245 601.0 0 245 139 250 601.0 0 250 140 1599 601.0 0 1599 141 1712 601.0 0 1712 142 1118 601.0 0 1118 143 153 601.0 0 153 144 159 601.0 0 159 145 203 601.0 0 203 146 174 601.0 0 174 147 278 601.0 0 278 148 247 601.0 0 247 149 421 601.0 0 421 150 184 601.0 0 184 151 198 601.0 0 198 152 231 601.0 0 231 153 275 601.0 0 275 154 1774 601.0 0 1774 155 684 601.0 0 684 156 686 601.0 0 686 157 5905 601.0 0 5905 158 4961 601.0 0 4961 159 7459 601.0 0 7459 160 204 601.0 0 204 161 156 601.0 0 156 162 182 601.0 0 182 163 160 601.0 0 160 164 215 601.0 0 215 165 170 601.0 0 170 166 175 601.0 0 175 167 152 601.0 0 152 168 139 601.0 0 139 169 164 601.0 0 164 170 123 601.0 0 123 171 143 601.0 0 143 172 139 601.0 0 139 173 134 601.0 0 134 174 139 601.0 0 139 175 156 601.0 0 156 176 112 601.0 0 112 177 135 601.0 0 135 178 123 601.0 0 123 179 138 601.0 0 138 180 133 601.0 0 133 181 142 601.0 0 142 182 138 601.0 0 138 183 108 601.0 0 108 184 101 601.0 0 101 185 124 601.0 0 124 186 135 601.0 0 135 187 117 601.0 0 117 188 139 601.0 0 139 189 111 601.0 0 111 190 152 601.0 0 152 191 184 601.0 0 184 192 202 601.0 0 202 193 144 601.0 0 144 194 107 601.0 0 107 195 107 601.0 0 107 196 153 601.0 0 153 197 126 601.0 0 126 198 103 601.0 0 103 199 145 601.0 0 145 200 122 601.0 0 122 201 137 601.0 0 137 202 150 601.0 0 150 203 118 601.0 0 118 204 134 601.0 0 134 205 127 601.0 0 127 206 147 601.0 0 147 207 150 601.0 0 150 208 127 601.0 0 127 209 112 601.0 0 112 210 117 601.0 0 117 211 114 601.0 0 114 212 120 601.0 0 120 213 136 601.0 0 136 214 135 601.0 0 135 215 135 601.0 0 135 216 131 601.0 0 131 217 114 601.0 0 114 218 102 601.0 0 102 219 134 601.0 0 134 220 164 601.0 0 164 221 132 601.0 0 132 222 170 601.0 0 170 223 125 601.0 0 125 224 120 601.0 0 120 225 121 601.0 0 121 226 268 601.0 0 268 227 133 601.0 0 133 228 150 601.0 0 150 229 129 601.0 0 129 230 118 601.0 0 118 231 133 601.0 0 133 232 157 601.0 0 157 233 124 601.0 0 124 234 146 601.0 0 146 235 159 601.0 0 159 236 125 601.0 0 125 237 125 601.0 0 125 238 121 601.0 0 121 239 132 601.0 0 132 240 133 601.0 0 133 241 114 601.0 0 114 242 137 601.0 0 137 243 117 601.0 0 117 244 167 601.0 0 167 245 118 601.0 0 118 246 117 601.0 0 117 247 134 601.0 0 134 248 110 601.0 0 110 249 107 601.0 0 107 250 117 601.0 0 117 251 136 601.0 0 136 252 214 601.0 0 214 253 247 601.0 0 247 254 273 601.0 0 273 255 102 601.0 0 102 256 135 601.0 0 135 257 151 601.0 0 151 258 118 601.0 0 118 259 98 601.0 0 98 260 155 601.0 0 155 261 130 601.0 0 130 262 165 601.0 0 165 263 115 601.0 0 115 264 163 601.0 0 163 265 104 601.0 0 104 266 111 601.0 0 111 267 125 601.0 0 125 268 225 601.0 0 225 269 445 601.0 0 445 270 683 601.0 0 683 271 469 601.0 0 469 272 614 601.0 0 614 273 165 601.0 0 165 274 191 601.0 0 191 275 211 601.0 0 211 276 765 601.0 0 765 277 1427 601.0 0 1427 278 2557 601.0 0 2557 279 1336 601.0 0 1336 280 215 601.0 0 215 281 197 601.0 0 197 282 285 601.0 0 285 283 163 601.0 0 163 284 175 601.0 0 175 285 256 601.0 0 256 286 181 601.0 0 181 287 147 601.0 0 147 288 194 601.0 0 194 289 366 601.0 0 366 290 325 601.0 0 325 291 332 601.0 0 332 292 378 601.0 0 378 293 436 601.0 0 436 294 797 601.0 0 797 295 19983 601.0 0 19983 296 9639 601.0 0 9639 297 4121 601.0 0 4121 298 122 601.0 0 122 299 121 601.0 0 121 300 111 601.0 0 111 301 115 601.0 0 115 Finished in 3.30 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 97,735 Reads with adapters: 2,646 (2.7%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 2,596 (2.7%) Total basepairs processed: 17,626,228 bp Total written (filtered): 676,808 bp (3.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2646 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 8 0.0 3 0 7 1 17 2275 0.0 3 1906 340 27 2 18 2 0.0 3 0 2 19 5 0.0 3 2 3 20 43 0.0 3 41 0 1 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 32 44 0.0 3 0 0 0 44 34 1 0.0 3 0 0 0 1 35 9 0.0 3 0 0 0 9 37 1 0.0 3 0 0 0 1 51 40 0.0 3 0 0 1 39 54 5 0.0 3 0 0 0 5 56 1 0.0 3 0 0 0 1 58 153 0.0 3 0 0 0 153 61 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 21 0.0 3 0 0 0 21 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 11 0.0 3 1 0 0 10 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 135 1 0.0 3 1 140 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 165 1 0.0 3 0 1 180 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 2,596 Reads with adapters: 8 (0.3%) Reads written (passing filters): 2,596 (100.0%) Total basepairs processed: 676,808 bp Total written (filtered): 676,779 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 8 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 25.0% G: 37.5% T: 37.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 40.6 0 6 5 1 2.5 0 1 6 1 0.6 0 1