This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.87 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 46,061 (1.9%) Reads that were too short: 18,371 (0.7%) Reads written (passing filters): 27,690 (1.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 4,342,520 bp (0.6%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 46061 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 108 601.0 0 108 7 3 601.0 0 3 8 61 601.0 0 61 9 23 601.0 0 23 10 26 601.0 0 26 11 7 601.0 0 7 12 4 601.0 0 4 13 42 601.0 0 42 14 171 601.0 0 171 15 199 601.0 0 199 16 26 601.0 0 26 17 20 601.0 0 20 18 58 601.0 0 58 19 87 601.0 0 87 20 115 601.0 0 115 21 20 601.0 0 20 22 2 601.0 0 2 23 3 601.0 0 3 24 3 601.0 0 3 25 6 601.0 0 6 26 26 601.0 0 26 27 50 601.0 0 50 28 12 601.0 0 12 29 4 601.0 0 4 30 5 601.0 0 5 31 2 601.0 0 2 32 1 601.0 0 1 33 15 601.0 0 15 34 6 601.0 0 6 35 8 601.0 0 8 36 5 601.0 0 5 38 9 601.0 0 9 39 5 601.0 0 5 40 7 601.0 0 7 41 15 601.0 0 15 42 12 601.0 0 12 43 115 601.0 0 115 44 249 601.0 0 249 45 341 601.0 0 341 46 172 601.0 0 172 47 14 601.0 0 14 48 6 601.0 0 6 49 12 601.0 0 12 50 13 601.0 0 13 51 25 601.0 0 25 52 18 601.0 0 18 53 12 601.0 0 12 54 30 601.0 0 30 55 10 601.0 0 10 56 58 601.0 0 58 57 11 601.0 0 11 58 16 601.0 0 16 59 18 601.0 0 18 60 29 601.0 0 29 61 15 601.0 0 15 62 15 601.0 0 15 63 12 601.0 0 12 64 17 601.0 0 17 65 25 601.0 0 25 66 28 601.0 0 28 67 46 601.0 0 46 68 44 601.0 0 44 69 19 601.0 0 19 70 20 601.0 0 20 71 41 601.0 0 41 72 22 601.0 0 22 73 52 601.0 0 52 74 47 601.0 0 47 75 39 601.0 0 39 76 154 601.0 0 154 77 731 601.0 0 731 78 1460 601.0 0 1460 79 2211 601.0 0 2211 80 43 601.0 0 43 81 10 601.0 0 10 82 45 601.0 0 45 83 12 601.0 0 12 84 31 601.0 0 31 85 23 601.0 0 23 86 24 601.0 0 24 87 6 601.0 0 6 88 6 601.0 0 6 89 5 601.0 0 5 90 10 601.0 0 10 91 28 601.0 0 28 92 71 601.0 0 71 93 22 601.0 0 22 94 49 601.0 0 49 95 39 601.0 0 39 96 97 601.0 0 97 97 22 601.0 0 22 98 2 601.0 0 2 99 6 601.0 0 6 100 17 601.0 0 17 101 16 601.0 0 16 102 16 601.0 0 16 103 28 601.0 0 28 104 53 601.0 0 53 105 20 601.0 0 20 106 9 601.0 0 9 107 13 601.0 0 13 108 19 601.0 0 19 109 18 601.0 0 18 110 21 601.0 0 21 111 14 601.0 0 14 112 25 601.0 0 25 113 35 601.0 0 35 114 224 601.0 0 224 115 129 601.0 0 129 116 144 601.0 0 144 117 60 601.0 0 60 118 10 601.0 0 10 119 17 601.0 0 17 120 26 601.0 0 26 121 51 601.0 0 51 122 35 601.0 0 35 123 46 601.0 0 46 124 33 601.0 0 33 125 33 601.0 0 33 126 46 601.0 0 46 127 37 601.0 0 37 128 34 601.0 0 34 129 100 601.0 0 100 130 25 601.0 0 25 131 19 601.0 0 19 132 22 601.0 0 22 133 61 601.0 0 61 134 25 601.0 0 25 135 85 601.0 0 85 136 348 601.0 0 348 137 312 601.0 0 312 138 242 601.0 0 242 139 112 601.0 0 112 140 309 601.0 0 309 141 34 601.0 0 34 142 62 601.0 0 62 143 27 601.0 0 27 144 31 601.0 0 31 145 28 601.0 0 28 146 32 601.0 0 32 147 21 601.0 0 21 148 56 601.0 0 56 149 55 601.0 0 55 150 104 601.0 0 104 151 49 601.0 0 49 152 66 601.0 0 66 153 26 601.0 0 26 154 56 601.0 0 56 155 28 601.0 0 28 156 22 601.0 0 22 157 49 601.0 0 49 158 17 601.0 0 17 159 28 601.0 0 28 160 33 601.0 0 33 161 34 601.0 0 34 162 60 601.0 0 60 163 89 601.0 0 89 164 90 601.0 0 90 165 122 601.0 0 122 166 120 601.0 0 120 167 33 601.0 0 33 168 21 601.0 0 21 169 38 601.0 0 38 170 38 601.0 0 38 171 45 601.0 0 45 172 69 601.0 0 69 173 1245 601.0 0 1245 174 641 601.0 0 641 175 703 601.0 0 703 176 63 601.0 0 63 177 56 601.0 0 56 178 55 601.0 0 55 179 135 601.0 0 135 180 801 601.0 0 801 181 770 601.0 0 770 182 1415 601.0 0 1415 183 88 601.0 0 88 184 69 601.0 0 69 185 77 601.0 0 77 186 60 601.0 0 60 187 60 601.0 0 60 188 114 601.0 0 114 189 219 601.0 0 219 190 394 601.0 0 394 191 1904 601.0 0 1904 192 2894 601.0 0 2894 193 3028 601.0 0 3028 194 373 601.0 0 373 195 18 601.0 0 18 196 18 601.0 0 18 197 25 601.0 0 25 198 29 601.0 0 29 199 30 601.0 0 30 200 27 601.0 0 27 201 43 601.0 0 43 202 256 601.0 0 256 203 381 601.0 0 381 204 576 601.0 0 576 205 552 601.0 0 552 206 71 601.0 0 71 207 20 601.0 0 20 208 204 601.0 0 204 209 245 601.0 0 245 210 186 601.0 0 186 211 63 601.0 0 63 212 18 601.0 0 18 213 19 601.0 0 19 214 21 601.0 0 21 215 24 601.0 0 24 216 59 601.0 0 59 217 97 601.0 0 97 218 89 601.0 0 89 219 44 601.0 0 44 220 14 601.0 0 14 221 19 601.0 0 19 222 22 601.0 0 22 223 26 601.0 0 26 224 53 601.0 0 53 225 63 601.0 0 63 226 37 601.0 0 37 227 78 601.0 0 78 228 30 601.0 0 30 229 42 601.0 0 42 230 12 601.0 0 12 231 52 601.0 0 52 232 66 601.0 0 66 233 98 601.0 0 98 234 68 601.0 0 68 235 74 601.0 0 74 236 78 601.0 0 78 237 34 601.0 0 34 238 33 601.0 0 33 239 26 601.0 0 26 240 37 601.0 0 37 241 24 601.0 0 24 242 32 601.0 0 32 243 17 601.0 0 17 244 34 601.0 0 34 245 37 601.0 0 37 246 43 601.0 0 43 247 35 601.0 0 35 248 40 601.0 0 40 249 29 601.0 0 29 250 27 601.0 0 27 251 65 601.0 0 65 252 397 601.0 0 397 253 485 601.0 0 485 254 377 601.0 0 377 255 61 601.0 0 61 256 25 601.0 0 25 257 29 601.0 0 29 258 269 601.0 0 269 259 412 601.0 0 412 260 257 601.0 0 257 261 483 601.0 0 483 262 492 601.0 0 492 263 171 601.0 0 171 264 207 601.0 0 207 265 280 601.0 0 280 266 175 601.0 0 175 267 73 601.0 0 73 268 31 601.0 0 31 269 40 601.0 0 40 270 91 601.0 0 91 271 106 601.0 0 106 272 135 601.0 0 135 273 105 601.0 0 105 274 77 601.0 0 77 275 90 601.0 0 90 276 60 601.0 0 60 277 69 601.0 0 69 278 69 601.0 0 69 279 118 601.0 0 118 280 150 601.0 0 150 281 103 601.0 0 103 282 86 601.0 0 86 283 399 601.0 0 399 284 3193 601.0 0 3193 285 1602 601.0 0 1602 286 1846 601.0 0 1846 287 142 601.0 0 142 288 187 601.0 0 187 289 66 601.0 0 66 290 73 601.0 0 73 291 106 601.0 0 106 292 65 601.0 0 65 293 72 601.0 0 72 294 58 601.0 0 58 295 156 601.0 0 156 296 187 601.0 0 187 297 168 601.0 0 168 298 78 601.0 0 78 299 43 601.0 0 43 300 27 601.0 0 27 301 110 601.0 0 110 Finished in 1.01 s (36 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 27,690 Reads with adapters: 35 (0.1%) Reads that were too short: 19 (0.1%) Reads written (passing filters): 16 (0.1%) Total basepairs processed: 4,342,520 bp Total written (filtered): 3,323 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 8 0.0 4 6 0 2 41 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 1 69 2 0.0 4 0 0 0 0 2 79 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 0 2 91 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (625 us/read; 0.10 M reads/minute). === Summary === Total reads processed: 16 Reads with adapters: 0 (0.0%) Reads written (passing filters): 16 (100.0%) Total basepairs processed: 3,323 bp Total written (filtered): 3,323 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.