This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.98 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 28,145 (1.1%) Reads that were too short: 7,647 (0.3%) Reads written (passing filters): 20,498 (0.8%) Total basepairs processed: 740,966,583 bp Total written (filtered): 3,142,025 bp (0.4%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 28145 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 93 601.0 0 93 7 3 601.0 0 3 8 50 601.0 0 50 9 9 601.0 0 9 10 2 601.0 0 2 11 1 601.0 0 1 12 1 601.0 0 1 13 2 601.0 0 2 14 3 601.0 0 3 15 3 601.0 0 3 16 3 601.0 0 3 17 1 601.0 0 1 18 5 601.0 0 5 19 14 601.0 0 14 20 17 601.0 0 17 21 14 601.0 0 14 22 2 601.0 0 2 23 1 601.0 0 1 24 1 601.0 0 1 25 1 601.0 0 1 26 1 601.0 0 1 27 2 601.0 0 2 28 3 601.0 0 3 29 2 601.0 0 2 30 4 601.0 0 4 31 3 601.0 0 3 32 2 601.0 0 2 33 21 601.0 0 21 34 1 601.0 0 1 35 6 601.0 0 6 36 11 601.0 0 11 37 5 601.0 0 5 38 3 601.0 0 3 39 1 601.0 0 1 40 6 601.0 0 6 41 6 601.0 0 6 42 13 601.0 0 13 43 15 601.0 0 15 44 31 601.0 0 31 45 48 601.0 0 48 46 46 601.0 0 46 47 9 601.0 0 9 48 18 601.0 0 18 49 14 601.0 0 14 50 14 601.0 0 14 51 22 601.0 0 22 52 20 601.0 0 20 53 17 601.0 0 17 54 20 601.0 0 20 55 17 601.0 0 17 56 36 601.0 0 36 57 7 601.0 0 7 58 12 601.0 0 12 59 22 601.0 0 22 60 27 601.0 0 27 61 9 601.0 0 9 62 4 601.0 0 4 63 16 601.0 0 16 64 13 601.0 0 13 65 21 601.0 0 21 66 23 601.0 0 23 67 13 601.0 0 13 68 20 601.0 0 20 69 8 601.0 0 8 70 16 601.0 0 16 71 37 601.0 0 37 72 24 601.0 0 24 73 27 601.0 0 27 74 44 601.0 0 44 75 39 601.0 0 39 76 106 601.0 0 106 77 661 601.0 0 661 78 1397 601.0 0 1397 79 2498 601.0 0 2498 80 25 601.0 0 25 81 8 601.0 0 8 82 47 601.0 0 47 83 13 601.0 0 13 84 8 601.0 0 8 85 5 601.0 0 5 86 10 601.0 0 10 87 12 601.0 0 12 88 4 601.0 0 4 89 6 601.0 0 6 90 9 601.0 0 9 91 19 601.0 0 19 92 37 601.0 0 37 93 14 601.0 0 14 94 33 601.0 0 33 95 24 601.0 0 24 96 84 601.0 0 84 97 10 601.0 0 10 98 5 601.0 0 5 99 8 601.0 0 8 100 12 601.0 0 12 101 15 601.0 0 15 102 6 601.0 0 6 103 28 601.0 0 28 104 59 601.0 0 59 105 18 601.0 0 18 106 7 601.0 0 7 107 14 601.0 0 14 108 10 601.0 0 10 109 8 601.0 0 8 110 12 601.0 0 12 111 7 601.0 0 7 112 20 601.0 0 20 113 18 601.0 0 18 114 136 601.0 0 136 115 90 601.0 0 90 116 96 601.0 0 96 117 54 601.0 0 54 118 7 601.0 0 7 119 19 601.0 0 19 120 11 601.0 0 11 121 41 601.0 0 41 122 16 601.0 0 16 123 61 601.0 0 61 124 18 601.0 0 18 125 12 601.0 0 12 126 17 601.0 0 17 127 6 601.0 0 6 128 14 601.0 0 14 129 108 601.0 0 108 130 3 601.0 0 3 131 9 601.0 0 9 132 13 601.0 0 13 133 56 601.0 0 56 134 17 601.0 0 17 135 23 601.0 0 23 136 218 601.0 0 218 137 175 601.0 0 175 138 216 601.0 0 216 139 92 601.0 0 92 140 275 601.0 0 275 141 14 601.0 0 14 142 20 601.0 0 20 143 12 601.0 0 12 144 24 601.0 0 24 145 4 601.0 0 4 146 6 601.0 0 6 147 6 601.0 0 6 148 20 601.0 0 20 149 18 601.0 0 18 150 95 601.0 0 95 151 34 601.0 0 34 152 86 601.0 0 86 153 16 601.0 0 16 154 16 601.0 0 16 155 15 601.0 0 15 156 11 601.0 0 11 157 40 601.0 0 40 158 4 601.0 0 4 159 24 601.0 0 24 160 7 601.0 0 7 161 31 601.0 0 31 162 23 601.0 0 23 163 46 601.0 0 46 164 48 601.0 0 48 165 33 601.0 0 33 166 51 601.0 0 51 167 9 601.0 0 9 168 21 601.0 0 21 169 15 601.0 0 15 170 14 601.0 0 14 171 26 601.0 0 26 172 34 601.0 0 34 173 442 601.0 0 442 174 207 601.0 0 207 175 199 601.0 0 199 176 37 601.0 0 37 177 29 601.0 0 29 178 24 601.0 0 24 179 65 601.0 0 65 180 321 601.0 0 321 181 323 601.0 0 323 182 488 601.0 0 488 183 28 601.0 0 28 184 25 601.0 0 25 185 27 601.0 0 27 186 29 601.0 0 29 187 71 601.0 0 71 188 68 601.0 0 68 189 122 601.0 0 122 190 322 601.0 0 322 191 2134 601.0 0 2134 192 3134 601.0 0 3134 193 3226 601.0 0 3226 194 146 601.0 0 146 195 14 601.0 0 14 196 10 601.0 0 10 197 15 601.0 0 15 198 10 601.0 0 10 199 13 601.0 0 13 200 19 601.0 0 19 201 22 601.0 0 22 202 259 601.0 0 259 203 156 601.0 0 156 204 280 601.0 0 280 205 196 601.0 0 196 206 70 601.0 0 70 207 18 601.0 0 18 208 102 601.0 0 102 209 35 601.0 0 35 210 43 601.0 0 43 211 19 601.0 0 19 212 9 601.0 0 9 213 5 601.0 0 5 214 8 601.0 0 8 215 12 601.0 0 12 216 15 601.0 0 15 217 21 601.0 0 21 218 13 601.0 0 13 219 13 601.0 0 13 220 6 601.0 0 6 221 6 601.0 0 6 222 31 601.0 0 31 223 10 601.0 0 10 224 4 601.0 0 4 225 20 601.0 0 20 226 13 601.0 0 13 227 20 601.0 0 20 228 15 601.0 0 15 229 30 601.0 0 30 230 10 601.0 0 10 231 39 601.0 0 39 232 10 601.0 0 10 233 26 601.0 0 26 234 18 601.0 0 18 235 21 601.0 0 21 236 33 601.0 0 33 237 13 601.0 0 13 238 11 601.0 0 11 239 12 601.0 0 12 240 26 601.0 0 26 241 17 601.0 0 17 242 13 601.0 0 13 243 12 601.0 0 12 244 16 601.0 0 16 245 28 601.0 0 28 246 18 601.0 0 18 247 10 601.0 0 10 248 13 601.0 0 13 249 9 601.0 0 9 250 13 601.0 0 13 251 24 601.0 0 24 252 131 601.0 0 131 253 174 601.0 0 174 254 102 601.0 0 102 255 20 601.0 0 20 256 20 601.0 0 20 257 13 601.0 0 13 258 141 601.0 0 141 259 211 601.0 0 211 260 119 601.0 0 119 261 310 601.0 0 310 262 466 601.0 0 466 263 54 601.0 0 54 264 117 601.0 0 117 265 142 601.0 0 142 266 88 601.0 0 88 267 23 601.0 0 23 268 16 601.0 0 16 269 13 601.0 0 13 270 16 601.0 0 16 271 20 601.0 0 20 272 25 601.0 0 25 273 24 601.0 0 24 274 16 601.0 0 16 275 24 601.0 0 24 276 17 601.0 0 17 277 18 601.0 0 18 278 22 601.0 0 22 279 37 601.0 0 37 280 42 601.0 0 42 281 36 601.0 0 36 282 24 601.0 0 24 283 163 601.0 0 163 284 1112 601.0 0 1112 285 615 601.0 0 615 286 632 601.0 0 632 287 40 601.0 0 40 288 355 601.0 0 355 289 28 601.0 0 28 290 45 601.0 0 45 291 44 601.0 0 44 292 23 601.0 0 23 293 12 601.0 0 12 294 19 601.0 0 19 295 61 601.0 0 61 296 60 601.0 0 60 297 56 601.0 0 56 298 24 601.0 0 24 299 19 601.0 0 19 300 9 601.0 0 9 301 28 601.0 0 28 Finished in 0.67 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 20,498 Reads with adapters: 221 (1.1%) Reads that were too short: 28 (0.1%) Reads written (passing filters): 193 (0.9%) Total basepairs processed: 3,142,025 bp Total written (filtered): 43,916 bp (1.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 221 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 42 0.0 3 39 3 18 21 0.0 3 0 0 0 21 20 92 0.0 3 91 1 21 4 0.0 3 0 0 0 4 30 6 0.0 3 0 0 0 6 31 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 39 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 74 1 0.0 3 1 77 1 0.0 3 0 0 0 1 81 16 0.0 3 15 1 90 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 138 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 1 185 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 193 Reads with adapters: 4 (2.1%) Reads written (passing filters): 193 (100.0%) Total basepairs processed: 43,916 bp Total written (filtered): 43,904 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 25.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 3.0 0 4