This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.01 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 282,386 (11.5%) Reads that were too short: 12,579 (0.5%) Reads written (passing filters): 269,807 (11.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 78,214,728 bp (10.6%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 282386 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2356 601.0 0 2356 7 148829 601.0 0 148829 8 108006 601.0 0 108006 9 96 601.0 0 96 10 19 601.0 0 19 11 11 601.0 0 11 12 14 601.0 0 14 13 15 601.0 0 15 14 18 601.0 0 18 15 16 601.0 0 16 16 17 601.0 0 17 17 18 601.0 0 18 18 31 601.0 0 31 19 40 601.0 0 40 20 65 601.0 0 65 21 17 601.0 0 17 22 21 601.0 0 21 23 26 601.0 0 26 24 8 601.0 0 8 25 16 601.0 0 16 26 31 601.0 0 31 27 21 601.0 0 21 28 28 601.0 0 28 29 18 601.0 0 18 30 23 601.0 0 23 31 25 601.0 0 25 32 19 601.0 0 19 33 33 601.0 0 33 34 38 601.0 0 38 35 62 601.0 0 62 36 243 601.0 0 243 37 467 601.0 0 467 38 146 601.0 0 146 39 35 601.0 0 35 40 24 601.0 0 24 41 27 601.0 0 27 42 28 601.0 0 28 43 385 601.0 0 385 44 903 601.0 0 903 45 572 601.0 0 572 46 21 601.0 0 21 47 8 601.0 0 8 48 17 601.0 0 17 49 19 601.0 0 19 50 18 601.0 0 18 51 19 601.0 0 19 52 24 601.0 0 24 53 21 601.0 0 21 54 23 601.0 0 23 55 34 601.0 0 34 56 39 601.0 0 39 57 39 601.0 0 39 58 26 601.0 0 26 59 18 601.0 0 18 60 15 601.0 0 15 61 33 601.0 0 33 62 20 601.0 0 20 63 22 601.0 0 22 64 21 601.0 0 21 65 16 601.0 0 16 66 37 601.0 0 37 67 17 601.0 0 17 68 25 601.0 0 25 69 20 601.0 0 20 70 41 601.0 0 41 71 136 601.0 0 136 72 26 601.0 0 26 73 46 601.0 0 46 74 26 601.0 0 26 75 20 601.0 0 20 76 17 601.0 0 17 77 18 601.0 0 18 78 38 601.0 0 38 79 33 601.0 0 33 80 23 601.0 0 23 81 22 601.0 0 22 82 16 601.0 0 16 83 22 601.0 0 22 84 28 601.0 0 28 85 30 601.0 0 30 86 20 601.0 0 20 87 24 601.0 0 24 88 25 601.0 0 25 89 26 601.0 0 26 90 27 601.0 0 27 91 26 601.0 0 26 92 30 601.0 0 30 93 23 601.0 0 23 94 30 601.0 0 30 95 27 601.0 0 27 96 59 601.0 0 59 97 15 601.0 0 15 98 13 601.0 0 13 99 22 601.0 0 22 100 15 601.0 0 15 101 15 601.0 0 15 102 12 601.0 0 12 103 23 601.0 0 23 104 12 601.0 0 12 105 31 601.0 0 31 106 23 601.0 0 23 107 25 601.0 0 25 108 40 601.0 0 40 109 27 601.0 0 27 110 16 601.0 0 16 111 21 601.0 0 21 112 37 601.0 0 37 113 30 601.0 0 30 114 27 601.0 0 27 115 37 601.0 0 37 116 19 601.0 0 19 117 13 601.0 0 13 118 26 601.0 0 26 119 23 601.0 0 23 120 32 601.0 0 32 121 32 601.0 0 32 122 70 601.0 0 70 123 34 601.0 0 34 124 19 601.0 0 19 125 50 601.0 0 50 126 121 601.0 0 121 127 68 601.0 0 68 128 143 601.0 0 143 129 67 601.0 0 67 130 59 601.0 0 59 131 93 601.0 0 93 132 92 601.0 0 92 133 47 601.0 0 47 134 47 601.0 0 47 135 306 601.0 0 306 136 225 601.0 0 225 137 300 601.0 0 300 138 38 601.0 0 38 139 76 601.0 0 76 140 102 601.0 0 102 141 167 601.0 0 167 142 98 601.0 0 98 143 88 601.0 0 88 144 41 601.0 0 41 145 44 601.0 0 44 146 42 601.0 0 42 147 61 601.0 0 61 148 173 601.0 0 173 149 102 601.0 0 102 150 95 601.0 0 95 151 37 601.0 0 37 152 47 601.0 0 47 153 64 601.0 0 64 154 35 601.0 0 35 155 53 601.0 0 53 156 47 601.0 0 47 157 33 601.0 0 33 158 42 601.0 0 42 159 51 601.0 0 51 160 25 601.0 0 25 161 45 601.0 0 45 162 26 601.0 0 26 163 30 601.0 0 30 164 33 601.0 0 33 165 34 601.0 0 34 166 39 601.0 0 39 167 60 601.0 0 60 168 46 601.0 0 46 169 84 601.0 0 84 170 35 601.0 0 35 171 33 601.0 0 33 172 33 601.0 0 33 173 36 601.0 0 36 174 25 601.0 0 25 175 37 601.0 0 37 176 33 601.0 0 33 177 39 601.0 0 39 178 28 601.0 0 28 179 32 601.0 0 32 180 27 601.0 0 27 181 51 601.0 0 51 182 81 601.0 0 81 183 44 601.0 0 44 184 42 601.0 0 42 185 55 601.0 0 55 186 50 601.0 0 50 187 53 601.0 0 53 188 35 601.0 0 35 189 38 601.0 0 38 190 43 601.0 0 43 191 84 601.0 0 84 192 31 601.0 0 31 193 43 601.0 0 43 194 50 601.0 0 50 195 62 601.0 0 62 196 43 601.0 0 43 197 34 601.0 0 34 198 27 601.0 0 27 199 33 601.0 0 33 200 42 601.0 0 42 201 45 601.0 0 45 202 60 601.0 0 60 203 50 601.0 0 50 204 27 601.0 0 27 205 30 601.0 0 30 206 52 601.0 0 52 207 115 601.0 0 115 208 170 601.0 0 170 209 151 601.0 0 151 210 124 601.0 0 124 211 63 601.0 0 63 212 193 601.0 0 193 213 81 601.0 0 81 214 159 601.0 0 159 215 143 601.0 0 143 216 261 601.0 0 261 217 51 601.0 0 51 218 28 601.0 0 28 219 57 601.0 0 57 220 51 601.0 0 51 221 86 601.0 0 86 222 64 601.0 0 64 223 51 601.0 0 51 224 51 601.0 0 51 225 67 601.0 0 67 226 71 601.0 0 71 227 50 601.0 0 50 228 87 601.0 0 87 229 81 601.0 0 81 230 132 601.0 0 132 231 53 601.0 0 53 232 70 601.0 0 70 233 149 601.0 0 149 234 153 601.0 0 153 235 155 601.0 0 155 236 91 601.0 0 91 237 107 601.0 0 107 238 40 601.0 0 40 239 41 601.0 0 41 240 51 601.0 0 51 241 47 601.0 0 47 242 62 601.0 0 62 243 59 601.0 0 59 244 54 601.0 0 54 245 56 601.0 0 56 246 49 601.0 0 49 247 30 601.0 0 30 248 29 601.0 0 29 249 30 601.0 0 30 250 39 601.0 0 39 251 49 601.0 0 49 252 64 601.0 0 64 253 53 601.0 0 53 254 68 601.0 0 68 255 66 601.0 0 66 256 72 601.0 0 72 257 57 601.0 0 57 258 67 601.0 0 67 259 93 601.0 0 93 260 97 601.0 0 97 261 78 601.0 0 78 262 73 601.0 0 73 263 53 601.0 0 53 264 65 601.0 0 65 265 64 601.0 0 64 266 51 601.0 0 51 267 69 601.0 0 69 268 143 601.0 0 143 269 387 601.0 0 387 270 315 601.0 0 315 271 353 601.0 0 353 272 114 601.0 0 114 273 111 601.0 0 111 274 120 601.0 0 120 275 172 601.0 0 172 276 120 601.0 0 120 277 79 601.0 0 79 278 63 601.0 0 63 279 135 601.0 0 135 280 149 601.0 0 149 281 264 601.0 0 264 282 133 601.0 0 133 283 104 601.0 0 104 284 79 601.0 0 79 285 105 601.0 0 105 286 90 601.0 0 90 287 65 601.0 0 65 288 111 601.0 0 111 289 96 601.0 0 96 290 152 601.0 0 152 291 100 601.0 0 100 292 102 601.0 0 102 293 197 601.0 0 197 294 797 601.0 0 797 295 1021 601.0 0 1021 296 606 601.0 0 606 297 311 601.0 0 311 298 154 601.0 0 154 299 97 601.0 0 97 300 167 601.0 0 167 301 457 601.0 0 457 Finished in 13.43 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 269,807 Reads with adapters: 112,741 (41.8%) Reads that were too short: 102 (0.0%) Reads written (passing filters): 112,639 (41.7%) Total basepairs processed: 78,214,728 bp Total written (filtered): 30,664,294 bp (39.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 112741 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 23 0.0 3 0 0 0 10 13 18 95 0.0 3 0 0 41 32 22 19 1822 0.0 3 0 1331 215 189 87 20 108820 0.0 4 98089 8058 1925 557 191 21 264 0.0 4 62 167 21 12 2 22 35 0.0 4 2 6 19 3 5 23 715 0.0 4 53 7 0 417 238 24 6 0.0 4 1 0 0 0 5 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 27 2 0.0 4 1 0 0 0 1 28 4 0.0 4 0 0 0 0 4 29 1 0.0 4 0 0 0 1 31 1 0.0 4 1 35 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 1 91 17 0.0 4 0 0 0 0 17 92 7 0.0 4 0 0 0 0 7 94 2 0.0 4 0 0 0 0 2 95 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 98 5 0.0 4 0 0 0 0 5 99 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 7 0.0 4 0 0 0 0 7 127 771 0.0 4 0 0 0 1 770 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 1 0 0 1 142 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 20 0.0 4 0 0 0 1 19 159 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 1 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 211 2 0.0 4 1 0 0 0 1 214 3 0.0 4 0 0 0 0 3 215 4 0.0 4 0 0 0 0 4 216 1 0.0 4 0 0 0 0 1 217 3 0.0 4 0 0 0 0 3 218 1 0.0 4 0 0 0 0 1 219 2 0.0 4 0 0 0 0 2 220 2 0.0 4 0 0 0 1 1 225 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 0 10 229 6 0.0 4 0 0 0 0 6 230 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 4 0.0 4 0 0 0 0 4 237 4 0.0 4 0 0 0 0 4 251 2 0.0 4 0 0 0 0 2 260 3 0.0 4 0 0 0 0 3 262 4 0.0 4 0 0 0 0 4 263 4 0.0 4 0 0 0 0 4 264 4 0.0 4 0 0 0 1 3 265 6 0.0 4 0 0 0 0 6 266 2 0.0 4 0 0 0 0 2 274 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 4 0.0 4 0 0 0 0 4 295 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.08 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 112,639 Reads with adapters: 21,143 (18.8%) Reads written (passing filters): 112,639 (100.0%) Total basepairs processed: 30,664,294 bp Total written (filtered): 30,407,810 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 21143 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.5% C: 11.8% G: 82.2% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 271 1760.0 0 271 4 39 440.0 0 39 5 2 110.0 0 2 7 9 6.9 0 9 8 3 1.7 0 3 9 215 0.4 0 12 203 10 6978 0.1 1 542 6436 11 13173 0.0 1 519 12654 12 220 0.0 1 2 218 13 30 0.0 1 0 30 14 13 0.0 1 0 13 15 7 0.0 1 0 7 108 2 0.0 1 2 143 1 0.0 1 1 148 7 0.0 1 4 3 149 1 0.0 1 1 151 37 0.0 1 25 12 152 4 0.0 1 1 3 154 1 0.0 1 1 159 1 0.0 1 1 163 1 0.0 1 1 178 1 0.0 1 1 180 1 0.0 1 1 190 3 0.0 1 0 3 208 1 0.0 1 1 209 100 0.0 1 79 21 210 4 0.0 1 2 2 211 3 0.0 1 3 221 2 0.0 1 0 2 228 1 0.0 1 1 229 1 0.0 1 1 259 1 0.0 1 1 260 9 0.0 1 8 1 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.