This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.54 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 284,998 (11.6%) Reads that were too short: 5,477 (0.2%) Reads written (passing filters): 279,521 (11.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 81,382,994 bp (11.0%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 284998 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1847 601.0 0 1847 7 119601 601.0 0 119601 8 151508 601.0 0 151508 9 130 601.0 0 130 10 22 601.0 0 22 11 12 601.0 0 12 12 14 601.0 0 14 13 8 601.0 0 8 14 8 601.0 0 8 15 25 601.0 0 25 16 23 601.0 0 23 17 20 601.0 0 20 18 29 601.0 0 29 19 22 601.0 0 22 20 34 601.0 0 34 21 18 601.0 0 18 22 11 601.0 0 11 23 23 601.0 0 23 24 16 601.0 0 16 25 22 601.0 0 22 26 14 601.0 0 14 27 18 601.0 0 18 28 17 601.0 0 17 29 20 601.0 0 20 30 21 601.0 0 21 31 19 601.0 0 19 32 24 601.0 0 24 33 26 601.0 0 26 34 29 601.0 0 29 35 21 601.0 0 21 36 127 601.0 0 127 37 118 601.0 0 118 38 89 601.0 0 89 39 19 601.0 0 19 40 12 601.0 0 12 41 25 601.0 0 25 42 23 601.0 0 23 43 79 601.0 0 79 44 104 601.0 0 104 45 101 601.0 0 101 46 12 601.0 0 12 47 24 601.0 0 24 48 19 601.0 0 19 49 41 601.0 0 41 50 20 601.0 0 20 51 29 601.0 0 29 52 30 601.0 0 30 53 33 601.0 0 33 54 32 601.0 0 32 55 29 601.0 0 29 56 28 601.0 0 28 57 40 601.0 0 40 58 44 601.0 0 44 59 40 601.0 0 40 60 22 601.0 0 22 61 29 601.0 0 29 62 26 601.0 0 26 63 20 601.0 0 20 64 19 601.0 0 19 65 20 601.0 0 20 66 30 601.0 0 30 67 26 601.0 0 26 68 17 601.0 0 17 69 31 601.0 0 31 70 27 601.0 0 27 71 165 601.0 0 165 72 34 601.0 0 34 73 28 601.0 0 28 74 20 601.0 0 20 75 31 601.0 0 31 76 28 601.0 0 28 77 25 601.0 0 25 78 26 601.0 0 26 79 26 601.0 0 26 80 14 601.0 0 14 81 28 601.0 0 28 82 21 601.0 0 21 83 28 601.0 0 28 84 29 601.0 0 29 85 23 601.0 0 23 86 13 601.0 0 13 87 30 601.0 0 30 88 24 601.0 0 24 89 20 601.0 0 20 90 26 601.0 0 26 91 26 601.0 0 26 92 17 601.0 0 17 93 32 601.0 0 32 94 35 601.0 0 35 95 43 601.0 0 43 96 42 601.0 0 42 97 13 601.0 0 13 98 11 601.0 0 11 99 26 601.0 0 26 100 19 601.0 0 19 101 25 601.0 0 25 102 24 601.0 0 24 103 30 601.0 0 30 104 21 601.0 0 21 105 23 601.0 0 23 106 25 601.0 0 25 107 14 601.0 0 14 108 30 601.0 0 30 109 15 601.0 0 15 110 18 601.0 0 18 111 26 601.0 0 26 112 26 601.0 0 26 113 23 601.0 0 23 114 15 601.0 0 15 115 44 601.0 0 44 116 25 601.0 0 25 117 11 601.0 0 11 118 28 601.0 0 28 119 17 601.0 0 17 120 25 601.0 0 25 121 17 601.0 0 17 122 50 601.0 0 50 123 21 601.0 0 21 124 7 601.0 0 7 125 23 601.0 0 23 126 76 601.0 0 76 127 24 601.0 0 24 128 61 601.0 0 61 129 48 601.0 0 48 130 38 601.0 0 38 131 74 601.0 0 74 132 95 601.0 0 95 133 49 601.0 0 49 134 50 601.0 0 50 135 161 601.0 0 161 136 124 601.0 0 124 137 156 601.0 0 156 138 25 601.0 0 25 139 101 601.0 0 101 140 74 601.0 0 74 141 134 601.0 0 134 142 48 601.0 0 48 143 61 601.0 0 61 144 27 601.0 0 27 145 34 601.0 0 34 146 26 601.0 0 26 147 43 601.0 0 43 148 88 601.0 0 88 149 65 601.0 0 65 150 49 601.0 0 49 151 21 601.0 0 21 152 23 601.0 0 23 153 65 601.0 0 65 154 25 601.0 0 25 155 29 601.0 0 29 156 23 601.0 0 23 157 10 601.0 0 10 158 37 601.0 0 37 159 24 601.0 0 24 160 25 601.0 0 25 161 23 601.0 0 23 162 21 601.0 0 21 163 19 601.0 0 19 164 20 601.0 0 20 165 27 601.0 0 27 166 21 601.0 0 21 167 27 601.0 0 27 168 22 601.0 0 22 169 26 601.0 0 26 170 18 601.0 0 18 171 16 601.0 0 16 172 24 601.0 0 24 173 24 601.0 0 24 174 18 601.0 0 18 175 25 601.0 0 25 176 20 601.0 0 20 177 17 601.0 0 17 178 26 601.0 0 26 179 28 601.0 0 28 180 25 601.0 0 25 181 21 601.0 0 21 182 42 601.0 0 42 183 14 601.0 0 14 184 25 601.0 0 25 185 32 601.0 0 32 186 24 601.0 0 24 187 24 601.0 0 24 188 26 601.0 0 26 189 18 601.0 0 18 190 20 601.0 0 20 191 86 601.0 0 86 192 26 601.0 0 26 193 26 601.0 0 26 194 26 601.0 0 26 195 31 601.0 0 31 196 45 601.0 0 45 197 25 601.0 0 25 198 22 601.0 0 22 199 13 601.0 0 13 200 20 601.0 0 20 201 22 601.0 0 22 202 30 601.0 0 30 203 21 601.0 0 21 204 16 601.0 0 16 205 12 601.0 0 12 206 77 601.0 0 77 207 54 601.0 0 54 208 77 601.0 0 77 209 55 601.0 0 55 210 68 601.0 0 68 211 31 601.0 0 31 212 148 601.0 0 148 213 30 601.0 0 30 214 52 601.0 0 52 215 66 601.0 0 66 216 105 601.0 0 105 217 24 601.0 0 24 218 10 601.0 0 10 219 25 601.0 0 25 220 27 601.0 0 27 221 46 601.0 0 46 222 27 601.0 0 27 223 31 601.0 0 31 224 29 601.0 0 29 225 52 601.0 0 52 226 26 601.0 0 26 227 23 601.0 0 23 228 45 601.0 0 45 229 27 601.0 0 27 230 63 601.0 0 63 231 19 601.0 0 19 232 34 601.0 0 34 233 37 601.0 0 37 234 42 601.0 0 42 235 51 601.0 0 51 236 27 601.0 0 27 237 32 601.0 0 32 238 22 601.0 0 22 239 18 601.0 0 18 240 39 601.0 0 39 241 22 601.0 0 22 242 35 601.0 0 35 243 26 601.0 0 26 244 37 601.0 0 37 245 37 601.0 0 37 246 32 601.0 0 32 247 9 601.0 0 9 248 21 601.0 0 21 249 23 601.0 0 23 250 20 601.0 0 20 251 20 601.0 0 20 252 22 601.0 0 22 253 36 601.0 0 36 254 45 601.0 0 45 255 30 601.0 0 30 256 38 601.0 0 38 257 31 601.0 0 31 258 26 601.0 0 26 259 29 601.0 0 29 260 42 601.0 0 42 261 31 601.0 0 31 262 29 601.0 0 29 263 27 601.0 0 27 264 39 601.0 0 39 265 46 601.0 0 46 266 36 601.0 0 36 267 51 601.0 0 51 268 41 601.0 0 41 269 137 601.0 0 137 270 112 601.0 0 112 271 119 601.0 0 119 272 58 601.0 0 58 273 44 601.0 0 44 274 58 601.0 0 58 275 79 601.0 0 79 276 53 601.0 0 53 277 41 601.0 0 41 278 34 601.0 0 34 279 91 601.0 0 91 280 89 601.0 0 89 281 206 601.0 0 206 282 51 601.0 0 51 283 44 601.0 0 44 284 36 601.0 0 36 285 73 601.0 0 73 286 27 601.0 0 27 287 27 601.0 0 27 288 56 601.0 0 56 289 42 601.0 0 42 290 66 601.0 0 66 291 39 601.0 0 39 292 48 601.0 0 48 293 71 601.0 0 71 294 256 601.0 0 256 295 364 601.0 0 364 296 203 601.0 0 203 297 133 601.0 0 133 298 49 601.0 0 49 299 52 601.0 0 52 300 54 601.0 0 54 301 166 601.0 0 166 Finished in 12.31 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 279,521 Reads with adapters: 117,029 (41.9%) Reads that were too short: 68 (0.0%) Reads written (passing filters): 116,961 (41.8%) Total basepairs processed: 81,382,994 bp Total written (filtered): 32,395,407 bp (39.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 117029 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 21 0.0 3 0 0 21 16 689 0.0 3 0 670 18 1 17 115866 0.0 3 113296 2488 74 8 18 214 0.0 3 23 184 5 2 19 43 0.0 3 0 0 39 4 20 105 0.0 3 0 0 0 105 39 1 0.0 3 1 45 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 105 1 0.0 3 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 1 2 189 1 0.0 3 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 9 0.0 3 0 0 0 9 206 1 0.0 3 0 0 0 1 207 10 0.0 3 0 0 0 10 217 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 227 1 0.0 3 1 238 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 1 270 2 0.0 3 0 0 0 2 279 8 0.0 3 0 0 0 8 280 4 0.0 3 0 0 0 4 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 283 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.07 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 116,961 Reads with adapters: 301 (0.3%) Reads written (passing filters): 116,961 (100.0%) Total basepairs processed: 32,395,407 bp Total written (filtered): 32,394,413 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 301 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.6% C: 38.5% G: 15.0% T: 20.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 234 1827.5 0 234 4 50 456.9 0 50 5 10 114.2 0 10 6 7 28.6 0 7