This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.58 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 83,408 (3.4%) Reads that were too short: 17,062 (0.7%) Reads written (passing filters): 66,346 (2.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 17,046,334 bp (2.3%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 83408 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1198 601.0 0 1198 7 17306 601.0 0 17306 8 511 601.0 0 511 9 25971 601.0 0 25971 10 72 601.0 0 72 11 64 601.0 0 64 12 50 601.0 0 50 13 90 601.0 0 90 14 66 601.0 0 66 15 46 601.0 0 46 16 73 601.0 0 73 17 145 601.0 0 145 18 73 601.0 0 73 19 61 601.0 0 61 20 44 601.0 0 44 21 62 601.0 0 62 22 56 601.0 0 56 23 80 601.0 0 80 24 60 601.0 0 60 25 51 601.0 0 51 26 43 601.0 0 43 27 49 601.0 0 49 28 64 601.0 0 64 29 58 601.0 0 58 30 61 601.0 0 61 31 74 601.0 0 74 32 41 601.0 0 41 33 63 601.0 0 63 34 45 601.0 0 45 35 68 601.0 0 68 36 50 601.0 0 50 37 50 601.0 0 50 38 50 601.0 0 50 39 70 601.0 0 70 40 51 601.0 0 51 41 60 601.0 0 60 42 244 601.0 0 244 43 145 601.0 0 145 44 152 601.0 0 152 45 53 601.0 0 53 46 43 601.0 0 43 47 57 601.0 0 57 48 69 601.0 0 69 49 74 601.0 0 74 50 80 601.0 0 80 51 65 601.0 0 65 52 69 601.0 0 69 53 49 601.0 0 49 54 49 601.0 0 49 55 49 601.0 0 49 56 44 601.0 0 44 57 47 601.0 0 47 58 71 601.0 0 71 59 52 601.0 0 52 60 47 601.0 0 47 61 59 601.0 0 59 62 51 601.0 0 51 63 45 601.0 0 45 64 61 601.0 0 61 65 108 601.0 0 108 66 127 601.0 0 127 67 88 601.0 0 88 68 60 601.0 0 60 69 73 601.0 0 73 70 59 601.0 0 59 71 48 601.0 0 48 72 57 601.0 0 57 73 83 601.0 0 83 74 95 601.0 0 95 75 159 601.0 0 159 76 108 601.0 0 108 77 115 601.0 0 115 78 62 601.0 0 62 79 352 601.0 0 352 80 184 601.0 0 184 81 132 601.0 0 132 82 33 601.0 0 33 83 44 601.0 0 44 84 48 601.0 0 48 85 45 601.0 0 45 86 52 601.0 0 52 87 61 601.0 0 61 88 47 601.0 0 47 89 63 601.0 0 63 90 67 601.0 0 67 91 76 601.0 0 76 92 209 601.0 0 209 93 577 601.0 0 577 94 1672 601.0 0 1672 95 79 601.0 0 79 96 72 601.0 0 72 97 54 601.0 0 54 98 60 601.0 0 60 99 50 601.0 0 50 100 57 601.0 0 57 101 61 601.0 0 61 102 32 601.0 0 32 103 47 601.0 0 47 104 54 601.0 0 54 105 64 601.0 0 64 106 59 601.0 0 59 107 51 601.0 0 51 108 77 601.0 0 77 109 61 601.0 0 61 110 82 601.0 0 82 111 59 601.0 0 59 112 180 601.0 0 180 113 56 601.0 0 56 114 63 601.0 0 63 115 65 601.0 0 65 116 59 601.0 0 59 117 60 601.0 0 60 118 77 601.0 0 77 119 124 601.0 0 124 120 84 601.0 0 84 121 106 601.0 0 106 122 68 601.0 0 68 123 272 601.0 0 272 124 551 601.0 0 551 125 643 601.0 0 643 126 81 601.0 0 81 127 69 601.0 0 69 128 83 601.0 0 83 129 385 601.0 0 385 130 302 601.0 0 302 131 155 601.0 0 155 132 175 601.0 0 175 133 50 601.0 0 50 134 59 601.0 0 59 135 42 601.0 0 42 136 60 601.0 0 60 137 54 601.0 0 54 138 56 601.0 0 56 139 67 601.0 0 67 140 70 601.0 0 70 141 62 601.0 0 62 142 106 601.0 0 106 143 63 601.0 0 63 144 42 601.0 0 42 145 37 601.0 0 37 146 44 601.0 0 44 147 70 601.0 0 70 148 52 601.0 0 52 149 54 601.0 0 54 150 37 601.0 0 37 151 34 601.0 0 34 152 62 601.0 0 62 153 56 601.0 0 56 154 53 601.0 0 53 155 58 601.0 0 58 156 68 601.0 0 68 157 55 601.0 0 55 158 72 601.0 0 72 159 35 601.0 0 35 160 53 601.0 0 53 161 51 601.0 0 51 162 70 601.0 0 70 163 67 601.0 0 67 164 58 601.0 0 58 165 63 601.0 0 63 166 63 601.0 0 63 167 151 601.0 0 151 168 95 601.0 0 95 169 134 601.0 0 134 170 51 601.0 0 51 171 40 601.0 0 40 172 57 601.0 0 57 173 180 601.0 0 180 174 79 601.0 0 79 175 103 601.0 0 103 176 87 601.0 0 87 177 73 601.0 0 73 178 518 601.0 0 518 179 409 601.0 0 409 180 382 601.0 0 382 181 127 601.0 0 127 182 76 601.0 0 76 183 58 601.0 0 58 184 303 601.0 0 303 185 90 601.0 0 90 186 110 601.0 0 110 187 51 601.0 0 51 188 82 601.0 0 82 189 53 601.0 0 53 190 61 601.0 0 61 191 60 601.0 0 60 192 83 601.0 0 83 193 119 601.0 0 119 194 183 601.0 0 183 195 259 601.0 0 259 196 165 601.0 0 165 197 93 601.0 0 93 198 363 601.0 0 363 199 503 601.0 0 503 200 594 601.0 0 594 201 379 601.0 0 379 202 198 601.0 0 198 203 120 601.0 0 120 204 95 601.0 0 95 205 220 601.0 0 220 206 264 601.0 0 264 207 419 601.0 0 419 208 212 601.0 0 212 209 102 601.0 0 102 210 65 601.0 0 65 211 71 601.0 0 71 212 49 601.0 0 49 213 53 601.0 0 53 214 66 601.0 0 66 215 107 601.0 0 107 216 125 601.0 0 125 217 81 601.0 0 81 218 125 601.0 0 125 219 388 601.0 0 388 220 301 601.0 0 301 221 363 601.0 0 363 222 369 601.0 0 369 223 287 601.0 0 287 224 1525 601.0 0 1525 225 104 601.0 0 104 226 73 601.0 0 73 227 73 601.0 0 73 228 147 601.0 0 147 229 89 601.0 0 89 230 110 601.0 0 110 231 69 601.0 0 69 232 63 601.0 0 63 233 49 601.0 0 49 234 72 601.0 0 72 235 58 601.0 0 58 236 83 601.0 0 83 237 85 601.0 0 85 238 208 601.0 0 208 239 248 601.0 0 248 240 845 601.0 0 845 241 855 601.0 0 855 242 1078 601.0 0 1078 243 68 601.0 0 68 244 73 601.0 0 73 245 57 601.0 0 57 246 97 601.0 0 97 247 138 601.0 0 138 248 109 601.0 0 109 249 101 601.0 0 101 250 168 601.0 0 168 251 187 601.0 0 187 252 199 601.0 0 199 253 99 601.0 0 99 254 90 601.0 0 90 255 125 601.0 0 125 256 92 601.0 0 92 257 69 601.0 0 69 258 73 601.0 0 73 259 52 601.0 0 52 260 72 601.0 0 72 261 152 601.0 0 152 262 377 601.0 0 377 263 172 601.0 0 172 264 54 601.0 0 54 265 60 601.0 0 60 266 61 601.0 0 61 267 54 601.0 0 54 268 48 601.0 0 48 269 74 601.0 0 74 270 90 601.0 0 90 271 91 601.0 0 91 272 68 601.0 0 68 273 68 601.0 0 68 274 70 601.0 0 70 275 77 601.0 0 77 276 69 601.0 0 69 277 129 601.0 0 129 278 93 601.0 0 93 279 117 601.0 0 117 280 75 601.0 0 75 281 62 601.0 0 62 282 83 601.0 0 83 283 139 601.0 0 139 284 127 601.0 0 127 285 98 601.0 0 98 286 115 601.0 0 115 287 134 601.0 0 134 288 383 601.0 0 383 289 454 601.0 0 454 290 386 601.0 0 386 291 194 601.0 0 194 292 167 601.0 0 167 293 111 601.0 0 111 294 77 601.0 0 77 295 118 601.0 0 118 296 128 601.0 0 128 297 113 601.0 0 113 298 75 601.0 0 75 299 111 601.0 0 111 300 141 601.0 0 141 301 64 601.0 0 64 Finished in 3.32 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 66,346 Reads with adapters: 17,960 (27.1%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 17,912 (27.0%) Total basepairs processed: 17,046,334 bp Total written (filtered): 4,900,926 bp (28.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17960 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 11 0.0 3 0 0 7 3 1 19 258 0.0 3 0 208 24 19 7 20 17475 0.0 4 15930 1224 242 62 17 21 43 0.0 4 5 31 4 2 1 22 8 0.0 4 0 0 2 2 4 23 44 0.0 4 0 1 0 29 14 26 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 35 4 0.0 4 0 0 0 0 4 42 2 0.0 4 1 0 0 0 1 43 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 86 10 0.0 4 0 0 0 0 10 90 1 0.0 4 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 1 1 118 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 42 0.0 4 0 0 0 0 42 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 1 148 2 0.0 4 0 0 0 0 2 153 3 0.0 4 0 0 0 0 3 184 1 0.0 4 0 0 0 0 1 194 20 0.0 4 0 0 0 0 20 196 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 230 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 254 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 17,912 Reads with adapters: 758 (4.2%) Reads written (passing filters): 17,912 (100.0%) Total basepairs processed: 4,900,926 bp Total written (filtered): 4,884,039 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 758 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.3% C: 21.0% G: 57.5% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 143 279.9 0 143 4 12 70.0 0 12 5 4 17.5 0 4 6 1 4.4 0 1 9 10 0.1 0 2 8 10 33 0.0 1 1 32 11 118 0.0 1 6 112 12 332 0.0 1 10 322 13 9 0.0 1 0 9 15 40 0.0 1 23 17 16 2 0.0 1 2 20 4 0.0 1 4 33 1 0.0 1 1 113 4 0.0 1 3 1 152 2 0.0 1 2 194 1 0.0 1 0 1 210 35 0.0 1 29 6 211 3 0.0 1 3 212 1 0.0 1 1 225 3 0.0 1 3