This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.14 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 317,566 (12.9%) Reads that were too short: 36,146 (1.5%) Reads written (passing filters): 281,420 (11.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 76,178,416 bp (10.3%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 317566 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2407 601.0 0 2407 7 54150 601.0 0 54150 8 2307 601.0 0 2307 9 29467 601.0 0 29467 10 4280 601.0 0 4280 11 131689 601.0 0 131689 12 269 601.0 0 269 13 113 601.0 0 113 14 90 601.0 0 90 15 105 601.0 0 105 16 85 601.0 0 85 17 69 601.0 0 69 18 76 601.0 0 76 19 99 601.0 0 99 20 125 601.0 0 125 21 72 601.0 0 72 22 78 601.0 0 78 23 69 601.0 0 69 24 71 601.0 0 71 25 92 601.0 0 92 26 110 601.0 0 110 27 118 601.0 0 118 28 288 601.0 0 288 29 123 601.0 0 123 30 281 601.0 0 281 31 84 601.0 0 84 32 99 601.0 0 99 33 78 601.0 0 78 34 112 601.0 0 112 35 61 601.0 0 61 36 94 601.0 0 94 37 110 601.0 0 110 38 89 601.0 0 89 39 105 601.0 0 105 40 104 601.0 0 104 41 152 601.0 0 152 42 101 601.0 0 101 43 67 601.0 0 67 44 103 601.0 0 103 45 83 601.0 0 83 46 95 601.0 0 95 47 94 601.0 0 94 48 109 601.0 0 109 49 130 601.0 0 130 50 218 601.0 0 218 51 97 601.0 0 97 52 96 601.0 0 96 53 100 601.0 0 100 54 92 601.0 0 92 55 94 601.0 0 94 56 124 601.0 0 124 57 100 601.0 0 100 58 87 601.0 0 87 59 75 601.0 0 75 60 102 601.0 0 102 61 114 601.0 0 114 62 138 601.0 0 138 63 103 601.0 0 103 64 97 601.0 0 97 65 92 601.0 0 92 66 85 601.0 0 85 67 99 601.0 0 99 68 89 601.0 0 89 69 118 601.0 0 118 70 399 601.0 0 399 71 111 601.0 0 111 72 350 601.0 0 350 73 99 601.0 0 99 74 86 601.0 0 86 75 95 601.0 0 95 76 115 601.0 0 115 77 114 601.0 0 114 78 124 601.0 0 124 79 300 601.0 0 300 80 459 601.0 0 459 81 724 601.0 0 724 82 84 601.0 0 84 83 251 601.0 0 251 84 692 601.0 0 692 85 1209 601.0 0 1209 86 97 601.0 0 97 87 83 601.0 0 83 88 80 601.0 0 80 89 133 601.0 0 133 90 114 601.0 0 114 91 73 601.0 0 73 92 84 601.0 0 84 93 96 601.0 0 96 94 72 601.0 0 72 95 106 601.0 0 106 96 104 601.0 0 104 97 251 601.0 0 251 98 202 601.0 0 202 99 756 601.0 0 756 100 451 601.0 0 451 101 127 601.0 0 127 102 194 601.0 0 194 103 139 601.0 0 139 104 109 601.0 0 109 105 177 601.0 0 177 106 350 601.0 0 350 107 200 601.0 0 200 108 181 601.0 0 181 109 207 601.0 0 207 110 321 601.0 0 321 111 351 601.0 0 351 112 695 601.0 0 695 113 14401 601.0 0 14401 114 7059 601.0 0 7059 115 2757 601.0 0 2757 116 127 601.0 0 127 117 86 601.0 0 86 118 117 601.0 0 117 119 125 601.0 0 125 120 93 601.0 0 93 121 92 601.0 0 92 122 95 601.0 0 95 123 119 601.0 0 119 124 196 601.0 0 196 125 158 601.0 0 158 126 139 601.0 0 139 127 196 601.0 0 196 128 268 601.0 0 268 129 1116 601.0 0 1116 130 1012 601.0 0 1012 131 1163 601.0 0 1163 132 103 601.0 0 103 133 166 601.0 0 166 134 77 601.0 0 77 135 274 601.0 0 274 136 114 601.0 0 114 137 154 601.0 0 154 138 161 601.0 0 161 139 315 601.0 0 315 140 289 601.0 0 289 141 184 601.0 0 184 142 351 601.0 0 351 143 413 601.0 0 413 144 132 601.0 0 132 145 115 601.0 0 115 146 146 601.0 0 146 147 70 601.0 0 70 148 124 601.0 0 124 149 97 601.0 0 97 150 102 601.0 0 102 151 71 601.0 0 71 152 94 601.0 0 94 153 107 601.0 0 107 154 381 601.0 0 381 155 538 601.0 0 538 156 432 601.0 0 432 157 390 601.0 0 390 158 198 601.0 0 198 159 93 601.0 0 93 160 82 601.0 0 82 161 82 601.0 0 82 162 86 601.0 0 86 163 119 601.0 0 119 164 84 601.0 0 84 165 118 601.0 0 118 166 73 601.0 0 73 167 260 601.0 0 260 168 256 601.0 0 256 169 148 601.0 0 148 170 307 601.0 0 307 171 87 601.0 0 87 172 103 601.0 0 103 173 74 601.0 0 74 174 72 601.0 0 72 175 89 601.0 0 89 176 91 601.0 0 91 177 77 601.0 0 77 178 89 601.0 0 89 179 193 601.0 0 193 180 62 601.0 0 62 181 71 601.0 0 71 182 70 601.0 0 70 183 93 601.0 0 93 184 85 601.0 0 85 185 95 601.0 0 95 186 90 601.0 0 90 187 97 601.0 0 97 188 91 601.0 0 91 189 100 601.0 0 100 190 120 601.0 0 120 191 112 601.0 0 112 192 122 601.0 0 122 193 76 601.0 0 76 194 81 601.0 0 81 195 86 601.0 0 86 196 148 601.0 0 148 197 100 601.0 0 100 198 93 601.0 0 93 199 89 601.0 0 89 200 325 601.0 0 325 201 126 601.0 0 126 202 183 601.0 0 183 203 87 601.0 0 87 204 107 601.0 0 107 205 123 601.0 0 123 206 100 601.0 0 100 207 77 601.0 0 77 208 75 601.0 0 75 209 121 601.0 0 121 210 134 601.0 0 134 211 399 601.0 0 399 212 233 601.0 0 233 213 263 601.0 0 263 214 151 601.0 0 151 215 135 601.0 0 135 216 233 601.0 0 233 217 124 601.0 0 124 218 102 601.0 0 102 219 66 601.0 0 66 220 80 601.0 0 80 221 84 601.0 0 84 222 89 601.0 0 89 223 80 601.0 0 80 224 93 601.0 0 93 225 119 601.0 0 119 226 112 601.0 0 112 227 110 601.0 0 110 228 979 601.0 0 979 229 643 601.0 0 643 230 378 601.0 0 378 231 154 601.0 0 154 232 169 601.0 0 169 233 145 601.0 0 145 234 233 601.0 0 233 235 335 601.0 0 335 236 400 601.0 0 400 237 212 601.0 0 212 238 97 601.0 0 97 239 95 601.0 0 95 240 117 601.0 0 117 241 119 601.0 0 119 242 107 601.0 0 107 243 182 601.0 0 182 244 102 601.0 0 102 245 98 601.0 0 98 246 181 601.0 0 181 247 281 601.0 0 281 248 261 601.0 0 261 249 171 601.0 0 171 250 135 601.0 0 135 251 85 601.0 0 85 252 141 601.0 0 141 253 127 601.0 0 127 254 124 601.0 0 124 255 199 601.0 0 199 256 194 601.0 0 194 257 336 601.0 0 336 258 157 601.0 0 157 259 115 601.0 0 115 260 71 601.0 0 71 261 119 601.0 0 119 262 149 601.0 0 149 263 132 601.0 0 132 264 121 601.0 0 121 265 109 601.0 0 109 266 63 601.0 0 63 267 94 601.0 0 94 268 261 601.0 0 261 269 394 601.0 0 394 270 384 601.0 0 384 271 235 601.0 0 235 272 158 601.0 0 158 273 232 601.0 0 232 274 242 601.0 0 242 275 131 601.0 0 131 276 158 601.0 0 158 277 74 601.0 0 74 278 171 601.0 0 171 279 179 601.0 0 179 280 134 601.0 0 134 281 348 601.0 0 348 282 205 601.0 0 205 283 177 601.0 0 177 284 176 601.0 0 176 285 173 601.0 0 173 286 170 601.0 0 170 287 140 601.0 0 140 288 217 601.0 0 217 289 188 601.0 0 188 290 195 601.0 0 195 291 195 601.0 0 195 292 203 601.0 0 203 293 260 601.0 0 260 294 773 601.0 0 773 295 1262 601.0 0 1262 296 3142 601.0 0 3142 297 2725 601.0 0 2725 298 4013 601.0 0 4013 299 4328 601.0 0 4328 300 2782 601.0 0 2782 301 311 601.0 0 311 Finished in 13.94 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 281,420 Reads with adapters: 29,918 (10.6%) Reads that were too short: 86 (0.0%) Reads written (passing filters): 29,832 (10.6%) Total basepairs processed: 76,178,416 bp Total written (filtered): 8,111,213 bp (10.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29918 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 11 0.0 3 0 0 0 6 5 18 25 0.0 3 0 0 12 8 5 19 411 0.0 3 0 344 32 25 10 20 29147 0.0 4 26739 1860 401 109 38 21 62 0.0 4 8 44 5 2 3 22 3 0.0 4 0 1 1 1 23 124 0.0 4 1 0 0 84 39 24 2 0.0 4 0 0 0 0 2 26 2 0.0 4 0 0 1 0 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 33 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 1 46 1 0.0 4 1 50 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 82 3 0.0 4 1 0 0 0 2 85 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 1 1 93 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 0 0 0 2 99 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 103 5 0.0 4 0 0 0 0 5 106 1 0.0 4 0 0 0 0 1 117 1 0.0 4 1 121 21 0.0 4 0 0 0 0 21 123 2 0.0 4 0 0 0 0 2 127 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 1 138 1 0.0 4 0 1 139 2 0.0 4 0 0 0 1 1 140 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 1 145 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 5 0.0 4 0 0 0 0 5 152 4 0.0 4 0 1 0 0 3 153 4 0.0 4 0 0 0 0 4 154 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 1 169 3 0.0 4 0 0 0 0 3 170 2 0.0 4 0 0 0 0 2 176 2 0.0 4 0 0 0 1 1 186 1 0.0 4 0 0 0 0 1 187 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 1 200 1 0.0 4 0 1 204 1 0.0 4 0 1 208 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 1 225 1 0.0 4 0 1 226 1 0.0 4 0 0 1 227 1 0.0 4 0 0 1 228 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 249 2 0.0 4 0 0 0 0 2 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 262 4 0.0 4 0 0 0 0 4 265 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 29,832 Reads with adapters: 7,108 (23.8%) Reads written (passing filters): 29,832 (100.0%) Total basepairs processed: 8,111,213 bp Total written (filtered): 8,026,576 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7108 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 10.3% G: 84.3% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 466.1 0 24 4 7 116.5 0 7 5 3 29.1 0 3 6 1 7.3 0 1 8 3 0.5 0 3 9 1305 0.1 0 142 1163 10 4947 0.0 1 380 4567 11 702 0.0 1 31 671 12 15 0.0 1 0 15 13 11 0.0 1 0 11 14 1 0.0 1 0 1 15 1 0.0 1 0 1 22 5 0.0 1 2 3 23 1 0.0 1 1 28 1 0.0 1 1 38 1 0.0 1 0 1 94 6 0.0 1 5 1 120 11 0.0 1 9 2 138 1 0.0 1 0 1 149 1 0.0 1 1 189 1 0.0 1 0 1 199 1 0.0 1 1 203 1 0.0 1 1 208 40 0.0 1 35 5 209 1 0.0 1 1 210 1 0.0 1 1 211 12 0.0 1 9 3 226 1 0.0 1 0 1 234 1 0.0 1 1 243 1 0.0 1 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.