This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.09 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 348,190 (14.1%) Reads that were too short: 27,831 (1.1%) Reads written (passing filters): 320,359 (13.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 87,686,444 bp (11.8%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 348190 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2280 601.0 0 2280 7 45645 601.0 0 45645 8 2004 601.0 0 2004 9 41411 601.0 0 41411 10 3547 601.0 0 3547 11 171223 601.0 0 171223 12 254 601.0 0 254 13 92 601.0 0 92 14 82 601.0 0 82 15 83 601.0 0 83 16 65 601.0 0 65 17 72 601.0 0 72 18 66 601.0 0 66 19 73 601.0 0 73 20 80 601.0 0 80 21 60 601.0 0 60 22 71 601.0 0 71 23 67 601.0 0 67 24 78 601.0 0 78 25 72 601.0 0 72 26 87 601.0 0 87 27 69 601.0 0 69 28 99 601.0 0 99 29 90 601.0 0 90 30 266 601.0 0 266 31 76 601.0 0 76 32 71 601.0 0 71 33 79 601.0 0 79 34 89 601.0 0 89 35 82 601.0 0 82 36 101 601.0 0 101 37 95 601.0 0 95 38 80 601.0 0 80 39 129 601.0 0 129 40 93 601.0 0 93 41 199 601.0 0 199 42 122 601.0 0 122 43 71 601.0 0 71 44 101 601.0 0 101 45 83 601.0 0 83 46 111 601.0 0 111 47 97 601.0 0 97 48 119 601.0 0 119 49 120 601.0 0 120 50 229 601.0 0 229 51 82 601.0 0 82 52 88 601.0 0 88 53 103 601.0 0 103 54 89 601.0 0 89 55 126 601.0 0 126 56 97 601.0 0 97 57 99 601.0 0 99 58 74 601.0 0 74 59 90 601.0 0 90 60 80 601.0 0 80 61 98 601.0 0 98 62 116 601.0 0 116 63 118 601.0 0 118 64 118 601.0 0 118 65 101 601.0 0 101 66 91 601.0 0 91 67 80 601.0 0 80 68 94 601.0 0 94 69 95 601.0 0 95 70 400 601.0 0 400 71 130 601.0 0 130 72 381 601.0 0 381 73 87 601.0 0 87 74 101 601.0 0 101 75 85 601.0 0 85 76 89 601.0 0 89 77 90 601.0 0 90 78 101 601.0 0 101 79 202 601.0 0 202 80 464 601.0 0 464 81 434 601.0 0 434 82 107 601.0 0 107 83 277 601.0 0 277 84 824 601.0 0 824 85 1387 601.0 0 1387 86 90 601.0 0 90 87 93 601.0 0 93 88 98 601.0 0 98 89 109 601.0 0 109 90 91 601.0 0 91 91 94 601.0 0 94 92 91 601.0 0 91 93 91 601.0 0 91 94 94 601.0 0 94 95 90 601.0 0 90 96 94 601.0 0 94 97 197 601.0 0 197 98 183 601.0 0 183 99 738 601.0 0 738 100 449 601.0 0 449 101 158 601.0 0 158 102 185 601.0 0 185 103 156 601.0 0 156 104 134 601.0 0 134 105 172 601.0 0 172 106 338 601.0 0 338 107 188 601.0 0 188 108 224 601.0 0 224 109 191 601.0 0 191 110 301 601.0 0 301 111 272 601.0 0 272 112 473 601.0 0 473 113 13803 601.0 0 13803 114 6129 601.0 0 6129 115 2630 601.0 0 2630 116 142 601.0 0 142 117 75 601.0 0 75 118 156 601.0 0 156 119 102 601.0 0 102 120 91 601.0 0 91 121 81 601.0 0 81 122 95 601.0 0 95 123 137 601.0 0 137 124 166 601.0 0 166 125 143 601.0 0 143 126 122 601.0 0 122 127 125 601.0 0 125 128 188 601.0 0 188 129 1094 601.0 0 1094 130 858 601.0 0 858 131 1158 601.0 0 1158 132 109 601.0 0 109 133 115 601.0 0 115 134 92 601.0 0 92 135 217 601.0 0 217 136 92 601.0 0 92 137 105 601.0 0 105 138 91 601.0 0 91 139 133 601.0 0 133 140 117 601.0 0 117 141 81 601.0 0 81 142 254 601.0 0 254 143 344 601.0 0 344 144 122 601.0 0 122 145 75 601.0 0 75 146 139 601.0 0 139 147 86 601.0 0 86 148 112 601.0 0 112 149 82 601.0 0 82 150 119 601.0 0 119 151 60 601.0 0 60 152 95 601.0 0 95 153 134 601.0 0 134 154 444 601.0 0 444 155 709 601.0 0 709 156 587 601.0 0 587 157 487 601.0 0 487 158 273 601.0 0 273 159 104 601.0 0 104 160 86 601.0 0 86 161 67 601.0 0 67 162 82 601.0 0 82 163 139 601.0 0 139 164 83 601.0 0 83 165 128 601.0 0 128 166 107 601.0 0 107 167 333 601.0 0 333 168 268 601.0 0 268 169 129 601.0 0 129 170 441 601.0 0 441 171 135 601.0 0 135 172 102 601.0 0 102 173 105 601.0 0 105 174 104 601.0 0 104 175 95 601.0 0 95 176 83 601.0 0 83 177 88 601.0 0 88 178 105 601.0 0 105 179 223 601.0 0 223 180 80 601.0 0 80 181 71 601.0 0 71 182 83 601.0 0 83 183 80 601.0 0 80 184 100 601.0 0 100 185 91 601.0 0 91 186 93 601.0 0 93 187 107 601.0 0 107 188 96 601.0 0 96 189 120 601.0 0 120 190 95 601.0 0 95 191 92 601.0 0 92 192 92 601.0 0 92 193 87 601.0 0 87 194 111 601.0 0 111 195 79 601.0 0 79 196 97 601.0 0 97 197 102 601.0 0 102 198 100 601.0 0 100 199 104 601.0 0 104 200 305 601.0 0 305 201 123 601.0 0 123 202 161 601.0 0 161 203 88 601.0 0 88 204 97 601.0 0 97 205 134 601.0 0 134 206 96 601.0 0 96 207 72 601.0 0 72 208 81 601.0 0 81 209 101 601.0 0 101 210 108 601.0 0 108 211 664 601.0 0 664 212 110 601.0 0 110 213 118 601.0 0 118 214 92 601.0 0 92 215 77 601.0 0 77 216 178 601.0 0 178 217 178 601.0 0 178 218 105 601.0 0 105 219 93 601.0 0 93 220 85 601.0 0 85 221 99 601.0 0 99 222 115 601.0 0 115 223 109 601.0 0 109 224 92 601.0 0 92 225 119 601.0 0 119 226 107 601.0 0 107 227 92 601.0 0 92 228 346 601.0 0 346 229 239 601.0 0 239 230 178 601.0 0 178 231 140 601.0 0 140 232 125 601.0 0 125 233 102 601.0 0 102 234 127 601.0 0 127 235 177 601.0 0 177 236 184 601.0 0 184 237 134 601.0 0 134 238 58 601.0 0 58 239 121 601.0 0 121 240 92 601.0 0 92 241 91 601.0 0 91 242 98 601.0 0 98 243 123 601.0 0 123 244 86 601.0 0 86 245 80 601.0 0 80 246 75 601.0 0 75 247 118 601.0 0 118 248 162 601.0 0 162 249 112 601.0 0 112 250 155 601.0 0 155 251 66 601.0 0 66 252 95 601.0 0 95 253 90 601.0 0 90 254 83 601.0 0 83 255 104 601.0 0 104 256 113 601.0 0 113 257 207 601.0 0 207 258 116 601.0 0 116 259 87 601.0 0 87 260 75 601.0 0 75 261 108 601.0 0 108 262 95 601.0 0 95 263 115 601.0 0 115 264 168 601.0 0 168 265 107 601.0 0 107 266 89 601.0 0 89 267 118 601.0 0 118 268 156 601.0 0 156 269 195 601.0 0 195 270 183 601.0 0 183 271 140 601.0 0 140 272 116 601.0 0 116 273 86 601.0 0 86 274 117 601.0 0 117 275 86 601.0 0 86 276 168 601.0 0 168 277 84 601.0 0 84 278 181 601.0 0 181 279 151 601.0 0 151 280 111 601.0 0 111 281 117 601.0 0 117 282 114 601.0 0 114 283 128 601.0 0 128 284 132 601.0 0 132 285 161 601.0 0 161 286 104 601.0 0 104 287 95 601.0 0 95 288 130 601.0 0 130 289 145 601.0 0 145 290 141 601.0 0 141 291 155 601.0 0 155 292 139 601.0 0 139 293 147 601.0 0 147 294 388 601.0 0 388 295 662 601.0 0 662 296 1545 601.0 0 1545 297 1038 601.0 0 1038 298 2684 601.0 0 2684 299 5805 601.0 0 5805 300 3736 601.0 0 3736 301 161 601.0 0 161 Finished in 13.62 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 320,359 Reads with adapters: 44,288 (13.8%) Reads that were too short: 145 (0.0%) Reads written (passing filters): 44,143 (13.8%) Total basepairs processed: 87,686,444 bp Total written (filtered): 12,212,143 bp (13.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 44288 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 7 1 16 202 0.0 3 0 198 3 1 17 43635 0.0 3 42625 979 27 4 18 97 0.0 3 12 81 4 19 22 0.0 3 1 0 21 20 55 0.0 3 0 0 0 55 21 1 0.0 3 0 0 0 1 23 4 0.0 3 0 0 0 4 25 1 0.0 3 1 30 2 0.0 3 2 32 10 0.0 3 0 0 0 10 33 1 0.0 3 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 2 0.0 3 1 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 50 17 0.0 3 0 0 0 17 52 1 0.0 3 1 53 7 0.0 3 0 0 0 7 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 1 2 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 73 38 0.0 3 0 0 35 3 77 1 0.0 3 0 0 0 1 80 3 0.0 3 0 0 0 3 83 1 0.0 3 0 1 94 7 0.0 3 0 0 0 7 99 1 0.0 3 1 102 2 0.0 3 0 1 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 107 1 0.0 3 0 0 0 1 110 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 126 1 0.0 3 1 128 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 4 0.0 3 0 1 0 3 167 1 0.0 3 1 172 2 0.0 3 1 1 174 1 0.0 3 1 176 1 0.0 3 1 178 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 190 3 0.0 3 0 0 1 2 191 1 0.0 3 1 192 4 0.0 3 0 0 0 4 200 25 0.0 3 0 0 0 25 203 5 0.0 3 0 0 0 5 205 3 0.0 3 0 0 0 3 215 18 0.0 3 0 0 0 18 219 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 236 10 0.0 3 0 0 0 10 266 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 277 4 0.0 3 0 0 0 4 278 5 0.0 3 0 0 0 5 279 5 0.0 3 0 0 0 5 280 9 0.0 3 0 0 0 9 281 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 44,143 Reads with adapters: 254 (0.6%) Reads written (passing filters): 44,143 (100.0%) Total basepairs processed: 12,212,143 bp Total written (filtered): 12,211,304 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 254 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.7% C: 25.6% G: 21.7% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 210 689.7 0 210 4 28 172.4 0 28 5 8 43.1 0 8 6 3 10.8 0 3 7 1 2.7 0 1 8 4 0.7 0 4