This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.62 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 129,285 (5.3%) Reads that were too short: 22,517 (0.9%) Reads written (passing filters): 106,768 (4.3%) Total basepairs processed: 740,966,583 bp Total written (filtered): 27,921,691 bp (3.8%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 129285 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 725 601.0 0 725 7 33148 601.0 0 33148 8 1453 601.0 0 1453 9 32415 601.0 0 32415 10 217 601.0 0 217 11 169 601.0 0 169 12 167 601.0 0 167 13 182 601.0 0 182 14 167 601.0 0 167 15 151 601.0 0 151 16 195 601.0 0 195 17 175 601.0 0 175 18 164 601.0 0 164 19 171 601.0 0 171 20 178 601.0 0 178 21 219 601.0 0 219 22 208 601.0 0 208 23 139 601.0 0 139 24 186 601.0 0 186 25 231 601.0 0 231 26 221 601.0 0 221 27 205 601.0 0 205 28 194 601.0 0 194 29 173 601.0 0 173 30 165 601.0 0 165 31 215 601.0 0 215 32 183 601.0 0 183 33 236 601.0 0 236 34 428 601.0 0 428 35 3615 601.0 0 3615 36 1491 601.0 0 1491 37 504 601.0 0 504 38 152 601.0 0 152 39 180 601.0 0 180 40 155 601.0 0 155 41 169 601.0 0 169 42 156 601.0 0 156 43 163 601.0 0 163 44 129 601.0 0 129 45 142 601.0 0 142 46 175 601.0 0 175 47 160 601.0 0 160 48 112 601.0 0 112 49 149 601.0 0 149 50 133 601.0 0 133 51 167 601.0 0 167 52 156 601.0 0 156 53 140 601.0 0 140 54 133 601.0 0 133 55 146 601.0 0 146 56 167 601.0 0 167 57 132 601.0 0 132 58 154 601.0 0 154 59 116 601.0 0 116 60 216 601.0 0 216 61 138 601.0 0 138 62 147 601.0 0 147 63 162 601.0 0 162 64 191 601.0 0 191 65 137 601.0 0 137 66 119 601.0 0 119 67 150 601.0 0 150 68 159 601.0 0 159 69 179 601.0 0 179 70 208 601.0 0 208 71 173 601.0 0 173 72 160 601.0 0 160 73 156 601.0 0 156 74 137 601.0 0 137 75 132 601.0 0 132 76 153 601.0 0 153 77 150 601.0 0 150 78 137 601.0 0 137 79 182 601.0 0 182 80 167 601.0 0 167 81 169 601.0 0 169 82 156 601.0 0 156 83 142 601.0 0 142 84 147 601.0 0 147 85 163 601.0 0 163 86 129 601.0 0 129 87 151 601.0 0 151 88 280 601.0 0 280 89 140 601.0 0 140 90 199 601.0 0 199 91 133 601.0 0 133 92 154 601.0 0 154 93 150 601.0 0 150 94 177 601.0 0 177 95 146 601.0 0 146 96 198 601.0 0 198 97 197 601.0 0 197 98 158 601.0 0 158 99 223 601.0 0 223 100 323 601.0 0 323 101 485 601.0 0 485 102 188 601.0 0 188 103 178 601.0 0 178 104 174 601.0 0 174 105 177 601.0 0 177 106 166 601.0 0 166 107 159 601.0 0 159 108 159 601.0 0 159 109 201 601.0 0 201 110 373 601.0 0 373 111 450 601.0 0 450 112 655 601.0 0 655 113 440 601.0 0 440 114 184 601.0 0 184 115 151 601.0 0 151 116 176 601.0 0 176 117 146 601.0 0 146 118 161 601.0 0 161 119 175 601.0 0 175 120 158 601.0 0 158 121 231 601.0 0 231 122 528 601.0 0 528 123 242 601.0 0 242 124 207 601.0 0 207 125 172 601.0 0 172 126 156 601.0 0 156 127 161 601.0 0 161 128 154 601.0 0 154 129 146 601.0 0 146 130 147 601.0 0 147 131 193 601.0 0 193 132 198 601.0 0 198 133 161 601.0 0 161 134 155 601.0 0 155 135 139 601.0 0 139 136 145 601.0 0 145 137 137 601.0 0 137 138 147 601.0 0 147 139 272 601.0 0 272 140 160 601.0 0 160 141 352 601.0 0 352 142 185 601.0 0 185 143 239 601.0 0 239 144 132 601.0 0 132 145 161 601.0 0 161 146 136 601.0 0 136 147 212 601.0 0 212 148 179 601.0 0 179 149 175 601.0 0 175 150 124 601.0 0 124 151 124 601.0 0 124 152 148 601.0 0 148 153 149 601.0 0 149 154 135 601.0 0 135 155 178 601.0 0 178 156 124 601.0 0 124 157 168 601.0 0 168 158 157 601.0 0 157 159 290 601.0 0 290 160 359 601.0 0 359 161 271 601.0 0 271 162 115 601.0 0 115 163 119 601.0 0 119 164 128 601.0 0 128 165 129 601.0 0 129 166 127 601.0 0 127 167 132 601.0 0 132 168 129 601.0 0 129 169 169 601.0 0 169 170 152 601.0 0 152 171 129 601.0 0 129 172 132 601.0 0 132 173 137 601.0 0 137 174 134 601.0 0 134 175 153 601.0 0 153 176 94 601.0 0 94 177 111 601.0 0 111 178 96 601.0 0 96 179 150 601.0 0 150 180 141 601.0 0 141 181 150 601.0 0 150 182 154 601.0 0 154 183 140 601.0 0 140 184 143 601.0 0 143 185 177 601.0 0 177 186 147 601.0 0 147 187 170 601.0 0 170 188 178 601.0 0 178 189 144 601.0 0 144 190 141 601.0 0 141 191 98 601.0 0 98 192 112 601.0 0 112 193 158 601.0 0 158 194 113 601.0 0 113 195 136 601.0 0 136 196 140 601.0 0 140 197 136 601.0 0 136 198 153 601.0 0 153 199 149 601.0 0 149 200 115 601.0 0 115 201 168 601.0 0 168 202 151 601.0 0 151 203 148 601.0 0 148 204 186 601.0 0 186 205 150 601.0 0 150 206 210 601.0 0 210 207 325 601.0 0 325 208 296 601.0 0 296 209 289 601.0 0 289 210 199 601.0 0 199 211 138 601.0 0 138 212 157 601.0 0 157 213 150 601.0 0 150 214 130 601.0 0 130 215 129 601.0 0 129 216 127 601.0 0 127 217 113 601.0 0 113 218 127 601.0 0 127 219 113 601.0 0 113 220 143 601.0 0 143 221 173 601.0 0 173 222 242 601.0 0 242 223 207 601.0 0 207 224 236 601.0 0 236 225 195 601.0 0 195 226 131 601.0 0 131 227 179 601.0 0 179 228 128 601.0 0 128 229 186 601.0 0 186 230 183 601.0 0 183 231 212 601.0 0 212 232 268 601.0 0 268 233 228 601.0 0 228 234 267 601.0 0 267 235 1422 601.0 0 1422 236 837 601.0 0 837 237 2884 601.0 0 2884 238 1504 601.0 0 1504 239 1320 601.0 0 1320 240 146 601.0 0 146 241 121 601.0 0 121 242 201 601.0 0 201 243 236 601.0 0 236 244 148 601.0 0 148 245 109 601.0 0 109 246 93 601.0 0 93 247 74 601.0 0 74 248 112 601.0 0 112 249 99 601.0 0 99 250 162 601.0 0 162 251 82 601.0 0 82 252 100 601.0 0 100 253 107 601.0 0 107 254 132 601.0 0 132 255 170 601.0 0 170 256 198 601.0 0 198 257 237 601.0 0 237 258 237 601.0 0 237 259 457 601.0 0 457 260 354 601.0 0 354 261 251 601.0 0 251 262 157 601.0 0 157 263 113 601.0 0 113 264 101 601.0 0 101 265 95 601.0 0 95 266 97 601.0 0 97 267 104 601.0 0 104 268 87 601.0 0 87 269 75 601.0 0 75 270 115 601.0 0 115 271 100 601.0 0 100 272 88 601.0 0 88 273 111 601.0 0 111 274 114 601.0 0 114 275 101 601.0 0 101 276 93 601.0 0 93 277 83 601.0 0 83 278 103 601.0 0 103 279 130 601.0 0 130 280 173 601.0 0 173 281 123 601.0 0 123 282 137 601.0 0 137 283 120 601.0 0 120 284 165 601.0 0 165 285 98 601.0 0 98 286 104 601.0 0 104 287 118 601.0 0 118 288 120 601.0 0 120 289 133 601.0 0 133 290 95 601.0 0 95 291 106 601.0 0 106 292 102 601.0 0 102 293 137 601.0 0 137 294 123 601.0 0 123 295 134 601.0 0 134 296 126 601.0 0 126 297 136 601.0 0 136 298 103 601.0 0 103 299 123 601.0 0 123 300 106 601.0 0 106 301 159 601.0 0 159 Finished in 5.30 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 106,768 Reads with adapters: 33,049 (31.0%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 33,007 (30.9%) Total basepairs processed: 27,921,691 bp Total written (filtered): 9,037,936 bp (32.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33049 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 15 0.0 3 0 0 7 5 3 19 440 0.0 3 0 359 47 29 5 20 32272 0.0 4 29685 2021 428 107 31 21 125 0.0 4 31 74 11 4 5 22 13 0.0 4 1 0 5 1 6 23 78 0.0 4 1 0 0 47 30 25 2 0.0 4 2 27 1 0.0 4 0 1 50 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 1 60 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 39 0.0 4 0 0 0 0 39 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 1 135 1 0.0 4 1 136 1 0.0 4 0 0 0 1 141 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 152 2 0.0 4 0 0 0 0 2 153 4 0.0 4 0 0 0 0 4 174 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 200 2 0.0 4 0 0 0 0 2 201 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 231 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 238 2 0.0 4 0 0 0 0 2 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.15 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 33,007 Reads with adapters: 2,020 (6.1%) Reads written (passing filters): 33,007 (100.0%) Total basepairs processed: 9,037,936 bp Total written (filtered): 9,001,161 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2020 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.1% C: 16.0% G: 70.0% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 189 515.7 0 189 4 26 128.9 0 26 5 2 32.2 0 2 6 1 8.1 0 1 8 1 0.5 0 1 9 18 0.1 0 4 14 10 23 0.0 1 0 23 11 1169 0.0 1 46 1123 12 485 0.0 1 5 480 13 12 0.0 1 0 12 14 1 0.0 1 0 1 15 7 0.0 1 0 7 16 1 0.0 1 0 1 152 15 0.0 1 13 2 153 2 0.0 1 1 1 178 1 0.0 1 0 1 179 3 0.0 1 3 191 4 0.0 1 0 4 203 1 0.0 1 0 1 208 1 0.0 1 1 210 56 0.0 1 48 8 211 1 0.0 1 0 1 271 1 0.0 1 1