This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.14 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 402,867 (16.4%) Reads that were too short: 11,291 (0.5%) Reads written (passing filters): 391,576 (15.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 104,668,849 bp (14.1%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 402867 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2400 601.0 0 2400 7 8861 601.0 0 8861 8 157270 601.0 0 157270 9 131206 601.0 0 131206 10 238 601.0 0 238 11 62 601.0 0 62 12 50 601.0 0 50 13 36 601.0 0 36 14 29 601.0 0 29 15 39 601.0 0 39 16 48 601.0 0 48 17 35 601.0 0 35 18 44 601.0 0 44 19 56 601.0 0 56 20 93 601.0 0 93 21 79 601.0 0 79 22 52 601.0 0 52 23 71 601.0 0 71 24 74 601.0 0 74 25 82 601.0 0 82 26 109 601.0 0 109 27 51 601.0 0 51 28 69 601.0 0 69 29 65 601.0 0 65 30 72 601.0 0 72 31 70 601.0 0 70 32 75 601.0 0 75 33 68 601.0 0 68 34 221 601.0 0 221 35 170 601.0 0 170 36 242 601.0 0 242 37 125 601.0 0 125 38 226 601.0 0 226 39 122 601.0 0 122 40 64 601.0 0 64 41 61 601.0 0 61 42 62 601.0 0 62 43 50 601.0 0 50 44 67 601.0 0 67 45 61 601.0 0 61 46 85 601.0 0 85 47 93 601.0 0 93 48 227 601.0 0 227 49 61 601.0 0 61 50 64 601.0 0 64 51 64 601.0 0 64 52 65 601.0 0 65 53 100 601.0 0 100 54 141 601.0 0 141 55 124 601.0 0 124 56 50 601.0 0 50 57 69 601.0 0 69 58 42 601.0 0 42 59 36 601.0 0 36 60 64 601.0 0 64 61 110 601.0 0 110 62 58 601.0 0 58 63 66 601.0 0 66 64 87 601.0 0 87 65 60 601.0 0 60 66 65 601.0 0 65 67 76 601.0 0 76 68 70 601.0 0 70 69 94 601.0 0 94 70 54 601.0 0 54 71 66 601.0 0 66 72 72 601.0 0 72 73 58 601.0 0 58 74 80 601.0 0 80 75 88 601.0 0 88 76 101 601.0 0 101 77 127 601.0 0 127 78 159 601.0 0 159 79 235 601.0 0 235 80 583 601.0 0 583 81 2885 601.0 0 2885 82 3518 601.0 0 3518 83 7246 601.0 0 7246 84 126 601.0 0 126 85 579 601.0 0 579 86 264 601.0 0 264 87 454 601.0 0 454 88 94 601.0 0 94 89 60 601.0 0 60 90 61 601.0 0 61 91 53 601.0 0 53 92 56 601.0 0 56 93 53 601.0 0 53 94 51 601.0 0 51 95 92 601.0 0 92 96 66 601.0 0 66 97 70 601.0 0 70 98 123 601.0 0 123 99 58 601.0 0 58 100 63 601.0 0 63 101 96 601.0 0 96 102 368 601.0 0 368 103 123 601.0 0 123 104 155 601.0 0 155 105 81 601.0 0 81 106 137 601.0 0 137 107 138 601.0 0 138 108 297 601.0 0 297 109 405 601.0 0 405 110 344 601.0 0 344 111 148 601.0 0 148 112 331 601.0 0 331 113 117 601.0 0 117 114 116 601.0 0 116 115 218 601.0 0 218 116 163 601.0 0 163 117 202 601.0 0 202 118 280 601.0 0 280 119 1372 601.0 0 1372 120 870 601.0 0 870 121 1259 601.0 0 1259 122 34179 601.0 0 34179 123 7228 601.0 0 7228 124 6669 601.0 0 6669 125 114 601.0 0 114 126 126 601.0 0 126 127 89 601.0 0 89 128 60 601.0 0 60 129 99 601.0 0 99 130 78 601.0 0 78 131 62 601.0 0 62 132 47 601.0 0 47 133 83 601.0 0 83 134 69 601.0 0 69 135 44 601.0 0 44 136 73 601.0 0 73 137 73 601.0 0 73 138 75 601.0 0 75 139 79 601.0 0 79 140 67 601.0 0 67 141 116 601.0 0 116 142 320 601.0 0 320 143 206 601.0 0 206 144 963 601.0 0 963 145 320 601.0 0 320 146 324 601.0 0 324 147 149 601.0 0 149 148 120 601.0 0 120 149 357 601.0 0 357 150 221 601.0 0 221 151 340 601.0 0 340 152 126 601.0 0 126 153 96 601.0 0 96 154 88 601.0 0 88 155 146 601.0 0 146 156 55 601.0 0 55 157 57 601.0 0 57 158 90 601.0 0 90 159 66 601.0 0 66 160 66 601.0 0 66 161 40 601.0 0 40 162 49 601.0 0 49 163 65 601.0 0 65 164 37 601.0 0 37 165 75 601.0 0 75 166 315 601.0 0 315 167 120 601.0 0 120 168 345 601.0 0 345 169 114 601.0 0 114 170 195 601.0 0 195 171 220 601.0 0 220 172 37 601.0 0 37 173 82 601.0 0 82 174 52 601.0 0 52 175 61 601.0 0 61 176 55 601.0 0 55 177 80 601.0 0 80 178 66 601.0 0 66 179 87 601.0 0 87 180 104 601.0 0 104 181 127 601.0 0 127 182 809 601.0 0 809 183 2160 601.0 0 2160 184 1632 601.0 0 1632 185 73 601.0 0 73 186 107 601.0 0 107 187 121 601.0 0 121 188 42 601.0 0 42 189 37 601.0 0 37 190 55 601.0 0 55 191 32 601.0 0 32 192 40 601.0 0 40 193 91 601.0 0 91 194 119 601.0 0 119 195 123 601.0 0 123 196 93 601.0 0 93 197 40 601.0 0 40 198 103 601.0 0 103 199 95 601.0 0 95 200 179 601.0 0 179 201 98 601.0 0 98 202 35 601.0 0 35 203 30 601.0 0 30 204 34 601.0 0 34 205 128 601.0 0 128 206 69 601.0 0 69 207 57 601.0 0 57 208 43 601.0 0 43 209 66 601.0 0 66 210 64 601.0 0 64 211 56 601.0 0 56 212 50 601.0 0 50 213 55 601.0 0 55 214 45 601.0 0 45 215 35 601.0 0 35 216 34 601.0 0 34 217 33 601.0 0 33 218 47 601.0 0 47 219 79 601.0 0 79 220 57 601.0 0 57 221 60 601.0 0 60 222 52 601.0 0 52 223 50 601.0 0 50 224 46 601.0 0 46 225 44 601.0 0 44 226 105 601.0 0 105 227 57 601.0 0 57 228 69 601.0 0 69 229 60 601.0 0 60 230 61 601.0 0 61 231 61 601.0 0 61 232 50 601.0 0 50 233 50 601.0 0 50 234 56 601.0 0 56 235 36 601.0 0 36 236 56 601.0 0 56 237 51 601.0 0 51 238 55 601.0 0 55 239 70 601.0 0 70 240 226 601.0 0 226 241 163 601.0 0 163 242 191 601.0 0 191 243 67 601.0 0 67 244 71 601.0 0 71 245 63 601.0 0 63 246 47 601.0 0 47 247 64 601.0 0 64 248 50 601.0 0 50 249 53 601.0 0 53 250 54 601.0 0 54 251 34 601.0 0 34 252 39 601.0 0 39 253 65 601.0 0 65 254 59 601.0 0 59 255 96 601.0 0 96 256 73 601.0 0 73 257 58 601.0 0 58 258 115 601.0 0 115 259 101 601.0 0 101 260 205 601.0 0 205 261 235 601.0 0 235 262 111 601.0 0 111 263 149 601.0 0 149 264 88 601.0 0 88 265 77 601.0 0 77 266 684 601.0 0 684 267 359 601.0 0 359 268 749 601.0 0 749 269 149 601.0 0 149 270 60 601.0 0 60 271 55 601.0 0 55 272 72 601.0 0 72 273 62 601.0 0 62 274 373 601.0 0 373 275 263 601.0 0 263 276 225 601.0 0 225 277 121 601.0 0 121 278 122 601.0 0 122 279 148 601.0 0 148 280 94 601.0 0 94 281 199 601.0 0 199 282 63 601.0 0 63 283 84 601.0 0 84 284 44 601.0 0 44 285 128 601.0 0 128 286 230 601.0 0 230 287 261 601.0 0 261 288 318 601.0 0 318 289 192 601.0 0 192 290 154 601.0 0 154 291 67 601.0 0 67 292 121 601.0 0 121 293 165 601.0 0 165 294 227 601.0 0 227 295 164 601.0 0 164 296 220 601.0 0 220 297 89 601.0 0 89 298 95 601.0 0 95 299 92 601.0 0 92 300 100 601.0 0 100 301 82 601.0 0 82 Finished in 18.26 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 391,576 Reads with adapters: 139,100 (35.5%) Reads that were too short: 142 (0.0%) Reads written (passing filters): 138,958 (35.5%) Total basepairs processed: 104,668,849 bp Total written (filtered): 37,780,148 bp (36.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 139100 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 17 0.0 3 0 0 0 8 9 18 134 0.0 3 0 0 93 27 14 19 2337 0.0 3 0 2037 159 95 46 20 133287 0.0 4 123185 7947 1577 446 132 21 293 0.0 4 39 216 19 16 3 22 2182 0.0 4 1979 136 41 9 17 23 451 0.0 4 1 2 2 303 143 24 5 0.0 4 1 1 0 1 2 25 12 0.0 4 0 0 0 10 2 26 2 0.0 4 0 0 0 1 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 1 32 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 47 7 0.0 4 4 2 0 0 1 48 1 0.0 4 1 52 1 0.0 4 1 58 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 4 0.0 4 0 0 0 0 4 93 3 0.0 4 0 0 0 0 3 94 2 0.0 4 0 0 0 0 2 99 6 0.0 4 0 0 0 1 5 100 2 0.0 4 0 0 0 0 2 101 3 0.0 4 0 0 0 0 3 102 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 181 0.0 4 0 0 0 0 181 130 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 139 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 149 4 0.0 4 0 0 0 1 3 150 10 0.0 4 0 0 0 1 9 151 3 0.0 4 0 0 0 0 3 152 5 0.0 4 0 0 0 0 5 153 14 0.0 4 0 0 0 1 13 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 4 0.0 4 1 0 0 0 3 162 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 7 0.0 4 0 0 0 0 7 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 0 3 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 11 0.0 4 0 0 0 0 11 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 233 3 0.0 4 0 0 0 0 3 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 256 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 262 5 0.0 4 0 0 0 0 5 264 3 0.0 4 0 0 0 0 3 265 3 0.0 4 0 0 0 0 3 273 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 292 4 0.0 4 0 0 0 1 3 293 5 0.0 4 0 0 0 1 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.09 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 138,958 Reads with adapters: 33,759 (24.3%) Reads written (passing filters): 138,958 (100.0%) Total basepairs processed: 37,780,148 bp Total written (filtered): 37,408,699 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 33759 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 10.1% G: 85.3% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 77 2171.2 0 77 4 36 542.8 0 36 5 2 135.7 0 2 6 7 33.9 0 7 7 15 8.5 0 15 8 8 2.1 0 8 9 8702 0.5 0 951 7751 10 20760 0.1 1 1397 19363 11 3760 0.0 1 120 3640 12 93 0.0 1 4 89 13 81 0.0 1 0 81 14 6 0.0 1 0 6 15 1 0.0 1 0 1 22 2 0.0 1 1 1 31 1 0.0 1 0 1 39 1 0.0 1 1 72 1 0.0 1 1 120 3 0.0 1 3 122 1 0.0 1 1 138 1 0.0 1 0 1 139 1 0.0 1 1 147 1 0.0 1 0 1 150 13 0.0 1 7 6 151 2 0.0 1 2 152 2 0.0 1 2 155 1 0.0 1 1 169 1 0.0 1 0 1 175 1 0.0 1 1 176 1 0.0 1 1 189 12 0.0 1 0 12 190 1 0.0 1 0 1 191 3 0.0 1 1 2 195 1 0.0 1 1 206 1 0.0 1 1 208 138 0.0 1 111 27 209 7 0.0 1 6 1 210 3 0.0 1 3 211 3 0.0 1 3 225 1 0.0 1 1 228 5 0.0 1 4 1 229 1 0.0 1 1 257 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.