This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.11 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 432,774 (17.6%) Reads that were too short: 10,007 (0.4%) Reads written (passing filters): 422,767 (17.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 113,163,400 bp (15.3%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 432774 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1815 601.0 0 1815 7 5782 601.0 0 5782 8 145921 601.0 0 145921 9 171255 601.0 0 171255 10 208 601.0 0 208 11 56 601.0 0 56 12 42 601.0 0 42 13 58 601.0 0 58 14 47 601.0 0 47 15 45 601.0 0 45 16 69 601.0 0 69 17 49 601.0 0 49 18 59 601.0 0 59 19 45 601.0 0 45 20 39 601.0 0 39 21 65 601.0 0 65 22 57 601.0 0 57 23 43 601.0 0 43 24 56 601.0 0 56 25 54 601.0 0 54 26 71 601.0 0 71 27 60 601.0 0 60 28 59 601.0 0 59 29 64 601.0 0 64 30 100 601.0 0 100 31 85 601.0 0 85 32 75 601.0 0 75 33 67 601.0 0 67 34 109 601.0 0 109 35 58 601.0 0 58 36 250 601.0 0 250 37 162 601.0 0 162 38 250 601.0 0 250 39 121 601.0 0 121 40 80 601.0 0 80 41 70 601.0 0 70 42 74 601.0 0 74 43 55 601.0 0 55 44 62 601.0 0 62 45 78 601.0 0 78 46 108 601.0 0 108 47 87 601.0 0 87 48 232 601.0 0 232 49 59 601.0 0 59 50 55 601.0 0 55 51 81 601.0 0 81 52 79 601.0 0 79 53 105 601.0 0 105 54 136 601.0 0 136 55 103 601.0 0 103 56 61 601.0 0 61 57 69 601.0 0 69 58 56 601.0 0 56 59 58 601.0 0 58 60 86 601.0 0 86 61 106 601.0 0 106 62 76 601.0 0 76 63 74 601.0 0 74 64 130 601.0 0 130 65 66 601.0 0 66 66 76 601.0 0 76 67 83 601.0 0 83 68 80 601.0 0 80 69 117 601.0 0 117 70 79 601.0 0 79 71 82 601.0 0 82 72 98 601.0 0 98 73 97 601.0 0 97 74 107 601.0 0 107 75 79 601.0 0 79 76 127 601.0 0 127 77 159 601.0 0 159 78 152 601.0 0 152 79 237 601.0 0 237 80 585 601.0 0 585 81 3664 601.0 0 3664 82 4400 601.0 0 4400 83 9532 601.0 0 9532 84 125 601.0 0 125 85 246 601.0 0 246 86 152 601.0 0 152 87 191 601.0 0 191 88 112 601.0 0 112 89 68 601.0 0 68 90 80 601.0 0 80 91 54 601.0 0 54 92 81 601.0 0 81 93 50 601.0 0 50 94 59 601.0 0 59 95 93 601.0 0 93 96 92 601.0 0 92 97 70 601.0 0 70 98 148 601.0 0 148 99 108 601.0 0 108 100 86 601.0 0 86 101 87 601.0 0 87 102 158 601.0 0 158 103 95 601.0 0 95 104 126 601.0 0 126 105 96 601.0 0 96 106 115 601.0 0 115 107 195 601.0 0 195 108 456 601.0 0 456 109 414 601.0 0 414 110 364 601.0 0 364 111 130 601.0 0 130 112 354 601.0 0 354 113 147 601.0 0 147 114 129 601.0 0 129 115 202 601.0 0 202 116 242 601.0 0 242 117 211 601.0 0 211 118 238 601.0 0 238 119 650 601.0 0 650 120 592 601.0 0 592 121 911 601.0 0 911 122 37696 601.0 0 37696 123 6800 601.0 0 6800 124 7169 601.0 0 7169 125 95 601.0 0 95 126 98 601.0 0 98 127 97 601.0 0 97 128 59 601.0 0 59 129 113 601.0 0 113 130 84 601.0 0 84 131 75 601.0 0 75 132 46 601.0 0 46 133 64 601.0 0 64 134 78 601.0 0 78 135 62 601.0 0 62 136 91 601.0 0 91 137 72 601.0 0 72 138 67 601.0 0 67 139 68 601.0 0 68 140 68 601.0 0 68 141 96 601.0 0 96 142 371 601.0 0 371 143 222 601.0 0 222 144 928 601.0 0 928 145 261 601.0 0 261 146 237 601.0 0 237 147 100 601.0 0 100 148 114 601.0 0 114 149 162 601.0 0 162 150 128 601.0 0 128 151 226 601.0 0 226 152 115 601.0 0 115 153 67 601.0 0 67 154 62 601.0 0 62 155 70 601.0 0 70 156 45 601.0 0 45 157 67 601.0 0 67 158 69 601.0 0 69 159 48 601.0 0 48 160 64 601.0 0 64 161 71 601.0 0 71 162 49 601.0 0 49 163 103 601.0 0 103 164 55 601.0 0 55 165 100 601.0 0 100 166 361 601.0 0 361 167 130 601.0 0 130 168 410 601.0 0 410 169 110 601.0 0 110 170 186 601.0 0 186 171 189 601.0 0 189 172 67 601.0 0 67 173 62 601.0 0 62 174 42 601.0 0 42 175 72 601.0 0 72 176 77 601.0 0 77 177 96 601.0 0 96 178 80 601.0 0 80 179 96 601.0 0 96 180 112 601.0 0 112 181 125 601.0 0 125 182 904 601.0 0 904 183 2706 601.0 0 2706 184 2059 601.0 0 2059 185 75 601.0 0 75 186 87 601.0 0 87 187 72 601.0 0 72 188 59 601.0 0 59 189 48 601.0 0 48 190 52 601.0 0 52 191 57 601.0 0 57 192 63 601.0 0 63 193 60 601.0 0 60 194 61 601.0 0 61 195 84 601.0 0 84 196 57 601.0 0 57 197 44 601.0 0 44 198 80 601.0 0 80 199 63 601.0 0 63 200 107 601.0 0 107 201 59 601.0 0 59 202 52 601.0 0 52 203 59 601.0 0 59 204 50 601.0 0 50 205 95 601.0 0 95 206 65 601.0 0 65 207 56 601.0 0 56 208 52 601.0 0 52 209 57 601.0 0 57 210 59 601.0 0 59 211 46 601.0 0 46 212 67 601.0 0 67 213 50 601.0 0 50 214 76 601.0 0 76 215 57 601.0 0 57 216 44 601.0 0 44 217 51 601.0 0 51 218 57 601.0 0 57 219 65 601.0 0 65 220 73 601.0 0 73 221 73 601.0 0 73 222 62 601.0 0 62 223 64 601.0 0 64 224 65 601.0 0 65 225 72 601.0 0 72 226 74 601.0 0 74 227 45 601.0 0 45 228 55 601.0 0 55 229 61 601.0 0 61 230 73 601.0 0 73 231 44 601.0 0 44 232 38 601.0 0 38 233 59 601.0 0 59 234 45 601.0 0 45 235 50 601.0 0 50 236 58 601.0 0 58 237 88 601.0 0 88 238 54 601.0 0 54 239 80 601.0 0 80 240 155 601.0 0 155 241 134 601.0 0 134 242 128 601.0 0 128 243 61 601.0 0 61 244 59 601.0 0 59 245 69 601.0 0 69 246 46 601.0 0 46 247 65 601.0 0 65 248 51 601.0 0 51 249 60 601.0 0 60 250 64 601.0 0 64 251 52 601.0 0 52 252 57 601.0 0 57 253 57 601.0 0 57 254 67 601.0 0 67 255 90 601.0 0 90 256 70 601.0 0 70 257 57 601.0 0 57 258 83 601.0 0 83 259 103 601.0 0 103 260 208 601.0 0 208 261 168 601.0 0 168 262 82 601.0 0 82 263 140 601.0 0 140 264 120 601.0 0 120 265 106 601.0 0 106 266 542 601.0 0 542 267 256 601.0 0 256 268 750 601.0 0 750 269 107 601.0 0 107 270 64 601.0 0 64 271 47 601.0 0 47 272 63 601.0 0 63 273 65 601.0 0 65 274 290 601.0 0 290 275 157 601.0 0 157 276 120 601.0 0 120 277 83 601.0 0 83 278 72 601.0 0 72 279 92 601.0 0 92 280 58 601.0 0 58 281 120 601.0 0 120 282 76 601.0 0 76 283 89 601.0 0 89 284 51 601.0 0 51 285 110 601.0 0 110 286 223 601.0 0 223 287 230 601.0 0 230 288 219 601.0 0 219 289 176 601.0 0 176 290 72 601.0 0 72 291 61 601.0 0 61 292 108 601.0 0 108 293 119 601.0 0 119 294 161 601.0 0 161 295 133 601.0 0 133 296 250 601.0 0 250 297 94 601.0 0 94 298 69 601.0 0 69 299 76 601.0 0 76 300 90 601.0 0 90 301 71 601.0 0 71 Finished in 17.46 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 422,767 Reads with adapters: 147,344 (34.9%) Reads that were too short: 540 (0.1%) Reads written (passing filters): 146,804 (34.7%) Total basepairs processed: 113,163,400 bp Total written (filtered): 40,364,033 bp (35.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 147344 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 30 0.0 3 0 0 30 16 687 0.0 3 0 674 13 17 144533 0.0 3 142091 2378 57 7 18 379 0.0 3 16 352 9 2 19 59 0.0 3 2 0 49 8 20 69 0.0 3 0 1 0 68 22 7 0.0 3 0 0 0 7 23 1 0.0 3 0 0 0 1 25 52 0.0 3 0 0 43 9 26 2 0.0 3 1 0 0 1 27 1 0.0 3 1 28 73 0.0 3 0 0 0 73 29 23 0.0 3 1 0 0 22 30 1 0.0 3 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 1 34 695 0.0 3 0 0 0 695 35 1 0.0 3 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 1 45 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 48 4 0.0 3 0 0 0 4 49 37 0.0 3 0 0 0 37 50 296 0.0 3 0 0 1 295 51 7 0.0 3 0 0 0 7 52 38 0.0 3 0 0 0 38 53 32 0.0 3 0 0 4 28 54 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 67 2 0.0 3 1 1 68 2 0.0 3 1 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 7 0.0 3 0 0 0 7 78 1 0.0 3 0 0 0 1 85 1 0.0 3 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 1 0 0 1 93 3 0.0 3 0 0 0 3 94 2 0.0 3 1 0 0 1 95 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 32 0.0 3 0 0 0 32 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 118 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 153 1 0.0 3 1 161 3 0.0 3 0 0 0 3 162 37 0.0 3 0 0 0 37 163 14 0.0 3 0 0 0 14 164 15 0.0 3 0 0 0 15 165 76 0.0 3 0 0 3 73 166 3 0.0 3 0 0 0 3 167 4 0.0 3 0 0 0 4 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 179 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 1 0.0 3 1 186 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 205 5 0.0 3 0 0 0 5 207 3 0.0 3 0 0 0 3 214 1 0.0 3 0 0 0 1 217 19 0.0 3 0 0 0 19 221 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 5 0.0 3 0 0 0 5 281 2 0.0 3 0 0 0 2 282 8 0.0 3 0 0 0 8 283 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.14 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 146,804 Reads with adapters: 2,639 (1.8%) Reads written (passing filters): 146,804 (100.0%) Total basepairs processed: 40,364,033 bp Total written (filtered): 40,355,567 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2639 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.0% C: 5.0% G: 17.5% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2096 2293.8 0 2096 4 539 573.5 0 539 5 2 143.4 0 2 6 2 35.8 0 2