This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.47 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 490,024 (19.9%) Reads that were too short: 357,809 (14.5%) Reads written (passing filters): 132,215 (5.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 26,645,891 bp (3.6%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 490024 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2685 601.0 0 2685 7 20506 601.0 0 20506 8 1308 601.0 0 1308 9 33336 601.0 0 33336 10 109 601.0 0 109 11 50 601.0 0 50 12 47 601.0 0 47 13 44 601.0 0 44 14 45 601.0 0 45 15 35 601.0 0 35 16 54 601.0 0 54 17 43 601.0 0 43 18 47 601.0 0 47 19 39 601.0 0 39 20 34 601.0 0 34 21 35 601.0 0 35 22 36 601.0 0 36 23 41 601.0 0 41 24 35 601.0 0 35 25 28 601.0 0 28 26 21 601.0 0 21 27 31 601.0 0 31 28 20 601.0 0 20 29 26 601.0 0 26 30 37 601.0 0 37 31 145 601.0 0 145 32 109 601.0 0 109 33 39 601.0 0 39 34 58 601.0 0 58 35 83 601.0 0 83 36 42 601.0 0 42 37 39 601.0 0 39 38 56 601.0 0 56 39 47 601.0 0 47 40 25 601.0 0 25 41 40 601.0 0 40 42 42 601.0 0 42 43 32 601.0 0 32 44 33 601.0 0 33 45 33 601.0 0 33 46 32 601.0 0 32 47 42 601.0 0 42 48 43 601.0 0 43 49 30 601.0 0 30 50 30 601.0 0 30 51 26 601.0 0 26 52 32 601.0 0 32 53 38 601.0 0 38 54 29 601.0 0 29 55 38 601.0 0 38 56 50 601.0 0 50 57 60 601.0 0 60 58 54 601.0 0 54 59 68 601.0 0 68 60 31 601.0 0 31 61 50 601.0 0 50 62 29 601.0 0 29 63 42 601.0 0 42 64 23 601.0 0 23 65 67 601.0 0 67 66 113 601.0 0 113 67 90 601.0 0 90 68 27 601.0 0 27 69 40 601.0 0 40 70 60 601.0 0 60 71 47 601.0 0 47 72 56 601.0 0 56 73 55 601.0 0 55 74 47 601.0 0 47 75 258 601.0 0 258 76 301 601.0 0 301 77 277 601.0 0 277 78 73 601.0 0 73 79 26 601.0 0 26 80 33 601.0 0 33 81 42 601.0 0 42 82 37 601.0 0 37 83 33 601.0 0 33 84 46 601.0 0 46 85 34 601.0 0 34 86 33 601.0 0 33 87 35 601.0 0 35 88 54 601.0 0 54 89 53 601.0 0 53 90 51 601.0 0 51 91 94 601.0 0 94 92 87 601.0 0 87 93 85 601.0 0 85 94 58 601.0 0 58 95 46 601.0 0 46 96 42 601.0 0 42 97 46 601.0 0 46 98 74 601.0 0 74 99 90 601.0 0 90 100 98 601.0 0 98 101 48 601.0 0 48 102 55 601.0 0 55 103 79 601.0 0 79 104 47 601.0 0 47 105 53 601.0 0 53 106 53 601.0 0 53 107 53 601.0 0 53 108 57 601.0 0 57 109 41 601.0 0 41 110 66 601.0 0 66 111 79 601.0 0 79 112 112 601.0 0 112 113 2253 601.0 0 2253 114 1355 601.0 0 1355 115 1254 601.0 0 1254 116 95 601.0 0 95 117 41 601.0 0 41 118 42 601.0 0 42 119 58 601.0 0 58 120 146 601.0 0 146 121 81 601.0 0 81 122 85 601.0 0 85 123 91 601.0 0 91 124 83 601.0 0 83 125 76 601.0 0 76 126 63 601.0 0 63 127 82 601.0 0 82 128 84 601.0 0 84 129 70 601.0 0 70 130 71 601.0 0 71 131 142 601.0 0 142 132 80 601.0 0 80 133 77 601.0 0 77 134 132 601.0 0 132 135 63 601.0 0 63 136 80 601.0 0 80 137 69 601.0 0 69 138 58 601.0 0 58 139 90 601.0 0 90 140 88 601.0 0 88 141 81 601.0 0 81 142 106 601.0 0 106 143 120 601.0 0 120 144 154 601.0 0 154 145 219 601.0 0 219 146 265 601.0 0 265 147 5878 601.0 0 5878 148 4245 601.0 0 4245 149 1240 601.0 0 1240 150 667 601.0 0 667 151 41 601.0 0 41 152 68 601.0 0 68 153 63 601.0 0 63 154 83 601.0 0 83 155 51 601.0 0 51 156 89 601.0 0 89 157 75 601.0 0 75 158 81 601.0 0 81 159 64 601.0 0 64 160 71 601.0 0 71 161 63 601.0 0 63 162 72 601.0 0 72 163 68 601.0 0 68 164 63 601.0 0 63 165 48 601.0 0 48 166 38 601.0 0 38 167 56 601.0 0 56 168 88 601.0 0 88 169 160 601.0 0 160 170 89 601.0 0 89 171 58 601.0 0 58 172 79 601.0 0 79 173 100 601.0 0 100 174 113 601.0 0 113 175 78 601.0 0 78 176 82 601.0 0 82 177 63 601.0 0 63 178 102 601.0 0 102 179 153 601.0 0 153 180 167 601.0 0 167 181 209 601.0 0 209 182 177 601.0 0 177 183 144 601.0 0 144 184 131 601.0 0 131 185 217 601.0 0 217 186 303 601.0 0 303 187 438 601.0 0 438 188 311 601.0 0 311 189 788 601.0 0 788 190 1158 601.0 0 1158 191 1219 601.0 0 1219 192 904 601.0 0 904 193 706 601.0 0 706 194 590 601.0 0 590 195 748 601.0 0 748 196 600 601.0 0 600 197 705 601.0 0 705 198 934 601.0 0 934 199 4108 601.0 0 4108 200 21085 601.0 0 21085 201 10717 601.0 0 10717 202 4773 601.0 0 4773 203 2163 601.0 0 2163 204 2933 601.0 0 2933 205 44135 601.0 0 44135 206 66228 601.0 0 66228 207 34619 601.0 0 34619 208 11395 601.0 0 11395 209 6343 601.0 0 6343 210 3252 601.0 0 3252 211 8808 601.0 0 8808 212 3015 601.0 0 3015 213 1905 601.0 0 1905 214 602 601.0 0 602 215 520 601.0 0 520 216 640 601.0 0 640 217 699 601.0 0 699 218 859 601.0 0 859 219 1198 601.0 0 1198 220 1626 601.0 0 1626 221 1856 601.0 0 1856 222 1987 601.0 0 1987 223 882 601.0 0 882 224 1723 601.0 0 1723 225 16227 601.0 0 16227 226 32625 601.0 0 32625 227 15819 601.0 0 15819 228 4182 601.0 0 4182 229 70 601.0 0 70 230 72 601.0 0 72 231 68 601.0 0 68 232 39 601.0 0 39 233 43 601.0 0 43 234 75 601.0 0 75 235 97 601.0 0 97 236 106 601.0 0 106 237 88 601.0 0 88 238 126 601.0 0 126 239 72 601.0 0 72 240 128 601.0 0 128 241 69 601.0 0 69 242 114 601.0 0 114 243 76 601.0 0 76 244 691 601.0 0 691 245 778 601.0 0 778 246 687 601.0 0 687 247 230 601.0 0 230 248 366 601.0 0 366 249 815 601.0 0 815 250 337 601.0 0 337 251 330 601.0 0 330 252 296 601.0 0 296 253 422 601.0 0 422 254 200 601.0 0 200 255 442 601.0 0 442 256 263 601.0 0 263 257 373 601.0 0 373 258 387 601.0 0 387 259 1046 601.0 0 1046 260 1680 601.0 0 1680 261 2476 601.0 0 2476 262 45977 601.0 0 45977 263 12820 601.0 0 12820 264 5494 601.0 0 5494 265 260 601.0 0 260 266 195 601.0 0 195 267 51 601.0 0 51 268 122 601.0 0 122 269 189 601.0 0 189 270 195 601.0 0 195 271 217 601.0 0 217 272 61 601.0 0 61 273 58 601.0 0 58 274 121 601.0 0 121 275 139 601.0 0 139 276 276 601.0 0 276 277 110 601.0 0 110 278 92 601.0 0 92 279 60 601.0 0 60 280 83 601.0 0 83 281 100 601.0 0 100 282 146 601.0 0 146 283 231 601.0 0 231 284 220 601.0 0 220 285 174 601.0 0 174 286 65 601.0 0 65 287 81 601.0 0 81 288 90 601.0 0 90 289 135 601.0 0 135 290 150 601.0 0 150 291 128 601.0 0 128 292 2420 601.0 0 2420 293 939 601.0 0 939 294 534 601.0 0 534 295 187 601.0 0 187 296 319 601.0 0 319 297 274 601.0 0 274 298 244 601.0 0 244 299 137 601.0 0 137 300 373 601.0 0 373 301 266 601.0 0 266 Finished in 5.70 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 132,215 Reads with adapters: 22,537 (17.0%) Reads that were too short: 70 (0.1%) Reads written (passing filters): 22,467 (17.0%) Total basepairs processed: 26,645,891 bp Total written (filtered): 6,147,621 bp (23.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22537 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 12 0.0 3 0 0 5 4 3 19 349 0.0 3 0 256 41 36 16 20 21829 0.0 4 19769 1573 355 95 37 21 105 0.0 4 44 49 7 1 4 22 5 0.0 4 0 0 2 2 1 23 67 0.0 4 0 0 0 53 14 24 5 0.0 4 1 0 0 1 3 25 1 0.0 4 0 0 1 27 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 0 0 0 1 34 3 0.0 4 0 0 0 0 3 36 4 0.0 4 1 1 0 0 2 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 64 3 0.0 4 0 0 0 0 3 66 3 0.0 4 0 0 0 0 3 77 2 0.0 4 0 0 0 0 2 88 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 5 0.0 4 0 0 0 0 5 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 102 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 1 119 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 60 0.0 4 0 0 0 1 59 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 1 140 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 6 0.0 4 0 0 0 0 6 158 1 0.0 4 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 2 0.0 4 0 0 0 0 2 195 1 0.0 4 0 0 0 1 200 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 1 222 2 0.0 4 0 1 0 0 1 227 1 0.0 4 0 0 1 228 7 0.0 4 1 0 1 1 4 229 3 0.0 4 0 0 0 0 3 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 1 1 242 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 253 2 0.0 4 0 1 0 0 1 262 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 22,467 Reads with adapters: 941 (4.2%) Reads written (passing filters): 22,467 (100.0%) Total basepairs processed: 6,147,621 bp Total written (filtered): 6,129,281 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 941 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.9% C: 21.7% G: 61.4% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 141 351.0 0 141 4 27 87.8 0 27 5 2 21.9 0 2 9 9 0.1 0 0 9 10 37 0.0 1 4 33 11 291 0.0 1 13 278 12 310 0.0 1 19 291 13 56 0.0 1 7 49 14 8 0.0 1 4 4 15 5 0.0 1 0 5 16 1 0.0 1 0 1 17 1 0.0 1 0 1 21 1 0.0 1 1 43 1 0.0 1 1 77 1 0.0 1 1 79 1 0.0 1 1 110 1 0.0 1 0 1 142 1 0.0 1 1 152 11 0.0 1 7 4 153 3 0.0 1 2 1 158 1 0.0 1 1 207 1 0.0 1 1 210 30 0.0 1 26 4 211 1 0.0 1 1