This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.18 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 494,977 (20.1%) Reads that were too short: 361,221 (14.7%) Reads written (passing filters): 133,756 (5.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 27,214,109 bp (3.7%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 494977 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3195 601.0 0 3195 7 27649 601.0 0 27649 8 776 601.0 0 776 9 29372 601.0 0 29372 10 86 601.0 0 86 11 57 601.0 0 57 12 47 601.0 0 47 13 56 601.0 0 56 14 41 601.0 0 41 15 23 601.0 0 23 16 40 601.0 0 40 17 52 601.0 0 52 18 40 601.0 0 40 19 42 601.0 0 42 20 34 601.0 0 34 21 52 601.0 0 52 22 38 601.0 0 38 23 25 601.0 0 25 24 33 601.0 0 33 25 27 601.0 0 27 26 25 601.0 0 25 27 23 601.0 0 23 28 28 601.0 0 28 29 40 601.0 0 40 30 21 601.0 0 21 31 90 601.0 0 90 32 108 601.0 0 108 33 40 601.0 0 40 34 75 601.0 0 75 35 101 601.0 0 101 36 59 601.0 0 59 37 55 601.0 0 55 38 86 601.0 0 86 39 44 601.0 0 44 40 31 601.0 0 31 41 55 601.0 0 55 42 25 601.0 0 25 43 33 601.0 0 33 44 32 601.0 0 32 45 52 601.0 0 52 46 36 601.0 0 36 47 49 601.0 0 49 48 28 601.0 0 28 49 32 601.0 0 32 50 32 601.0 0 32 51 22 601.0 0 22 52 30 601.0 0 30 53 42 601.0 0 42 54 33 601.0 0 33 55 41 601.0 0 41 56 51 601.0 0 51 57 58 601.0 0 58 58 42 601.0 0 42 59 62 601.0 0 62 60 54 601.0 0 54 61 51 601.0 0 51 62 31 601.0 0 31 63 33 601.0 0 33 64 29 601.0 0 29 65 54 601.0 0 54 66 37 601.0 0 37 67 41 601.0 0 41 68 27 601.0 0 27 69 39 601.0 0 39 70 58 601.0 0 58 71 39 601.0 0 39 72 37 601.0 0 37 73 62 601.0 0 62 74 43 601.0 0 43 75 244 601.0 0 244 76 217 601.0 0 217 77 200 601.0 0 200 78 39 601.0 0 39 79 42 601.0 0 42 80 31 601.0 0 31 81 40 601.0 0 40 82 40 601.0 0 40 83 39 601.0 0 39 84 39 601.0 0 39 85 29 601.0 0 29 86 29 601.0 0 29 87 44 601.0 0 44 88 53 601.0 0 53 89 51 601.0 0 51 90 41 601.0 0 41 91 60 601.0 0 60 92 51 601.0 0 51 93 43 601.0 0 43 94 41 601.0 0 41 95 47 601.0 0 47 96 50 601.0 0 50 97 64 601.0 0 64 98 71 601.0 0 71 99 64 601.0 0 64 100 99 601.0 0 99 101 56 601.0 0 56 102 47 601.0 0 47 103 81 601.0 0 81 104 56 601.0 0 56 105 54 601.0 0 54 106 66 601.0 0 66 107 65 601.0 0 65 108 51 601.0 0 51 109 42 601.0 0 42 110 53 601.0 0 53 111 59 601.0 0 59 112 79 601.0 0 79 113 1295 601.0 0 1295 114 883 601.0 0 883 115 890 601.0 0 890 116 79 601.0 0 79 117 64 601.0 0 64 118 45 601.0 0 45 119 48 601.0 0 48 120 136 601.0 0 136 121 92 601.0 0 92 122 95 601.0 0 95 123 84 601.0 0 84 124 92 601.0 0 92 125 68 601.0 0 68 126 65 601.0 0 65 127 76 601.0 0 76 128 55 601.0 0 55 129 69 601.0 0 69 130 65 601.0 0 65 131 137 601.0 0 137 132 54 601.0 0 54 133 86 601.0 0 86 134 130 601.0 0 130 135 97 601.0 0 97 136 79 601.0 0 79 137 81 601.0 0 81 138 56 601.0 0 56 139 86 601.0 0 86 140 86 601.0 0 86 141 88 601.0 0 88 142 108 601.0 0 108 143 104 601.0 0 104 144 136 601.0 0 136 145 198 601.0 0 198 146 216 601.0 0 216 147 5827 601.0 0 5827 148 4589 601.0 0 4589 149 1274 601.0 0 1274 150 706 601.0 0 706 151 43 601.0 0 43 152 49 601.0 0 49 153 42 601.0 0 42 154 49 601.0 0 49 155 71 601.0 0 71 156 67 601.0 0 67 157 67 601.0 0 67 158 65 601.0 0 65 159 47 601.0 0 47 160 102 601.0 0 102 161 71 601.0 0 71 162 90 601.0 0 90 163 61 601.0 0 61 164 54 601.0 0 54 165 61 601.0 0 61 166 48 601.0 0 48 167 44 601.0 0 44 168 78 601.0 0 78 169 170 601.0 0 170 170 63 601.0 0 63 171 48 601.0 0 48 172 63 601.0 0 63 173 79 601.0 0 79 174 79 601.0 0 79 175 68 601.0 0 68 176 85 601.0 0 85 177 66 601.0 0 66 178 97 601.0 0 97 179 164 601.0 0 164 180 144 601.0 0 144 181 232 601.0 0 232 182 186 601.0 0 186 183 163 601.0 0 163 184 167 601.0 0 167 185 189 601.0 0 189 186 306 601.0 0 306 187 438 601.0 0 438 188 299 601.0 0 299 189 736 601.0 0 736 190 1146 601.0 0 1146 191 1296 601.0 0 1296 192 1099 601.0 0 1099 193 788 601.0 0 788 194 549 601.0 0 549 195 775 601.0 0 775 196 593 601.0 0 593 197 613 601.0 0 613 198 995 601.0 0 995 199 3800 601.0 0 3800 200 21042 601.0 0 21042 201 11392 601.0 0 11392 202 4729 601.0 0 4729 203 2029 601.0 0 2029 204 2571 601.0 0 2571 205 47634 601.0 0 47634 206 78753 601.0 0 78753 207 38998 601.0 0 38998 208 11356 601.0 0 11356 209 6359 601.0 0 6359 210 3452 601.0 0 3452 211 9227 601.0 0 9227 212 3360 601.0 0 3360 213 2127 601.0 0 2127 214 524 601.0 0 524 215 467 601.0 0 467 216 767 601.0 0 767 217 836 601.0 0 836 218 1007 601.0 0 1007 219 1267 601.0 0 1267 220 1786 601.0 0 1786 221 1874 601.0 0 1874 222 2188 601.0 0 2188 223 878 601.0 0 878 224 1519 601.0 0 1519 225 13649 601.0 0 13649 226 35969 601.0 0 35969 227 18395 601.0 0 18395 228 5222 601.0 0 5222 229 51 601.0 0 51 230 45 601.0 0 45 231 28 601.0 0 28 232 46 601.0 0 46 233 38 601.0 0 38 234 48 601.0 0 48 235 61 601.0 0 61 236 76 601.0 0 76 237 43 601.0 0 43 238 91 601.0 0 91 239 53 601.0 0 53 240 89 601.0 0 89 241 59 601.0 0 59 242 64 601.0 0 64 243 29 601.0 0 29 244 199 601.0 0 199 245 250 601.0 0 250 246 195 601.0 0 195 247 139 601.0 0 139 248 214 601.0 0 214 249 396 601.0 0 396 250 132 601.0 0 132 251 175 601.0 0 175 252 200 601.0 0 200 253 228 601.0 0 228 254 133 601.0 0 133 255 479 601.0 0 479 256 185 601.0 0 185 257 200 601.0 0 200 258 229 601.0 0 229 259 466 601.0 0 466 260 980 601.0 0 980 261 1412 601.0 0 1412 262 38172 601.0 0 38172 263 11483 601.0 0 11483 264 4543 601.0 0 4543 265 104 601.0 0 104 266 65 601.0 0 65 267 22 601.0 0 22 268 60 601.0 0 60 269 65 601.0 0 65 270 71 601.0 0 71 271 80 601.0 0 80 272 24 601.0 0 24 273 16 601.0 0 16 274 99 601.0 0 99 275 100 601.0 0 100 276 100 601.0 0 100 277 41 601.0 0 41 278 36 601.0 0 36 279 23 601.0 0 23 280 21 601.0 0 21 281 25 601.0 0 25 282 46 601.0 0 46 283 87 601.0 0 87 284 54 601.0 0 54 285 45 601.0 0 45 286 25 601.0 0 25 287 30 601.0 0 30 288 37 601.0 0 37 289 57 601.0 0 57 290 100 601.0 0 100 291 57 601.0 0 57 292 405 601.0 0 405 293 222 601.0 0 222 294 149 601.0 0 149 295 50 601.0 0 50 296 117 601.0 0 117 297 85 601.0 0 85 298 59 601.0 0 59 299 46 601.0 0 46 300 351 601.0 0 351 301 73 601.0 0 73 Finished in 5.18 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 133,756 Reads with adapters: 30,589 (22.9%) Reads that were too short: 99 (0.1%) Reads written (passing filters): 30,490 (22.8%) Total basepairs processed: 27,214,109 bp Total written (filtered): 8,377,619 bp (30.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30589 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 13 0.0 3 0 0 13 16 275 0.0 3 0 271 4 17 30016 0.0 3 29420 573 20 3 18 67 0.0 3 11 48 3 5 19 20 0.0 3 0 0 18 2 20 34 0.0 3 2 0 0 32 23 1 0.0 3 1 24 1 0.0 3 1 26 20 0.0 3 0 0 0 20 30 3 0.0 3 0 0 0 3 31 1 0.0 3 1 33 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 38 3 0.0 3 3 49 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 69 2 0.0 3 1 0 0 1 75 53 0.0 3 0 0 1 52 77 1 0.0 3 0 0 0 1 78 17 0.0 3 1 0 0 16 79 4 0.0 3 0 0 0 4 80 2 0.0 3 0 0 0 2 81 5 0.0 3 0 0 0 5 89 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 119 1 0.0 3 1 129 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 226 1 0.0 3 0 1 236 1 0.0 3 0 0 1 237 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 30,490 Reads with adapters: 840 (2.8%) Reads written (passing filters): 30,490 (100.0%) Total basepairs processed: 8,377,619 bp Total written (filtered): 8,375,076 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 840 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.3% C: 6.1% G: 28.5% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 818 476.4 0 818 4 21 119.1 0 21 5 1 29.8 0 1