This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.76 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 135,769 (5.3%) Reads that were too short: 58,064 (2.3%) Reads written (passing filters): 77,705 (3.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 19,026,838 bp (2.5%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 135769 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 535 621.6 0 535 7 2492 621.6 0 2492 8 23550 621.6 0 23550 9 20375 621.6 0 20375 10 59 621.6 0 59 11 27 621.6 0 27 12 49 621.6 0 49 13 31 621.6 0 31 14 36 621.6 0 36 15 28 621.6 0 28 16 25 621.6 0 25 17 56 621.6 0 56 18 29 621.6 0 29 19 29 621.6 0 29 20 31 621.6 0 31 21 23 621.6 0 23 22 30 621.6 0 30 23 28 621.6 0 28 24 52 621.6 0 52 25 40 621.6 0 40 26 37 621.6 0 37 27 26 621.6 0 26 28 29 621.6 0 29 29 34 621.6 0 34 30 29 621.6 0 29 31 32 621.6 0 32 32 76 621.6 0 76 33 42 621.6 0 42 34 61 621.6 0 61 35 74 621.6 0 74 36 44 621.6 0 44 37 266 621.6 0 266 38 113 621.6 0 113 39 182 621.6 0 182 40 80 621.6 0 80 41 81 621.6 0 81 42 172 621.6 0 172 43 31 621.6 0 31 44 43 621.6 0 43 45 29 621.6 0 29 46 31 621.6 0 31 47 65 621.6 0 65 48 47 621.6 0 47 49 54 621.6 0 54 50 37 621.6 0 37 51 34 621.6 0 34 52 331 621.6 0 331 53 185 621.6 0 185 54 367 621.6 0 367 55 34 621.6 0 34 56 32 621.6 0 32 57 52 621.6 0 52 58 31 621.6 0 31 59 39 621.6 0 39 60 43 621.6 0 43 61 69 621.6 0 69 62 59 621.6 0 59 63 32 621.6 0 32 64 25 621.6 0 25 65 64 621.6 0 64 66 90 621.6 0 90 67 52 621.6 0 52 68 102 621.6 0 102 69 16 621.6 0 16 70 41 621.6 0 41 71 55 621.6 0 55 72 103 621.6 0 103 73 86 621.6 0 86 74 24 621.6 0 24 75 29 621.6 0 29 76 24 621.6 0 24 77 36 621.6 0 36 78 22 621.6 0 22 79 27 621.6 0 27 80 25 621.6 0 25 81 60 621.6 0 60 82 21 621.6 0 21 83 58 621.6 0 58 84 155 621.6 0 155 85 85 621.6 0 85 86 153 621.6 0 153 87 45 621.6 0 45 88 34 621.6 0 34 89 41 621.6 0 41 90 108 621.6 0 108 91 93 621.6 0 93 92 97 621.6 0 97 93 130 621.6 0 130 94 77 621.6 0 77 95 141 621.6 0 141 96 59 621.6 0 59 97 94 621.6 0 94 98 53 621.6 0 53 99 31 621.6 0 31 100 27 621.6 0 27 101 28 621.6 0 28 102 25 621.6 0 25 103 38 621.6 0 38 104 109 621.6 0 109 105 102 621.6 0 102 106 158 621.6 0 158 107 128 621.6 0 128 108 374 621.6 0 374 109 271 621.6 0 271 110 589 621.6 0 589 111 164 621.6 0 164 112 189 621.6 0 189 113 192 621.6 0 192 114 91 621.6 0 91 115 70 621.6 0 70 116 50 621.6 0 50 117 92 621.6 0 92 118 70 621.6 0 70 119 158 621.6 0 158 120 41 621.6 0 41 121 32 621.6 0 32 122 56 621.6 0 56 123 45 621.6 0 45 124 50 621.6 0 50 125 60 621.6 0 60 126 105 621.6 0 105 127 474 621.6 0 474 128 185 621.6 0 185 129 394 621.6 0 394 130 48 621.6 0 48 131 55 621.6 0 55 132 98 621.6 0 98 133 57 621.6 0 57 134 62 621.6 0 62 135 42 621.6 0 42 136 43 621.6 0 43 137 47 621.6 0 47 138 63 621.6 0 63 139 95 621.6 0 95 140 110 621.6 0 110 141 129 621.6 0 129 142 195 621.6 0 195 143 502 621.6 0 502 144 5077 621.6 0 5077 145 2482 621.6 0 2482 146 4403 621.6 0 4403 147 62 621.6 0 62 148 55 621.6 0 55 149 74 621.6 0 74 150 64 621.6 0 64 151 302 621.6 0 302 152 198 621.6 0 198 153 464 621.6 0 464 154 191 621.6 0 191 155 85 621.6 0 85 156 212 621.6 0 212 157 167 621.6 0 167 158 1552 621.6 0 1552 159 64 621.6 0 64 160 55 621.6 0 55 161 72 621.6 0 72 162 44 621.6 0 44 163 62 621.6 0 62 164 38 621.6 0 38 165 49 621.6 0 49 166 61 621.6 0 61 167 45 621.6 0 45 168 30 621.6 0 30 169 35 621.6 0 35 170 60 621.6 0 60 171 68 621.6 0 68 172 106 621.6 0 106 173 93 621.6 0 93 174 62 621.6 0 62 175 115 621.6 0 115 176 76 621.6 0 76 177 47 621.6 0 47 178 54 621.6 0 54 179 161 621.6 0 161 180 111 621.6 0 111 181 111 621.6 0 111 182 44 621.6 0 44 183 29 621.6 0 29 184 54 621.6 0 54 185 95 621.6 0 95 186 95 621.6 0 95 187 73 621.6 0 73 188 52 621.6 0 52 189 50 621.6 0 50 190 98 621.6 0 98 191 117 621.6 0 117 192 376 621.6 0 376 193 66 621.6 0 66 194 74 621.6 0 74 195 38 621.6 0 38 196 64 621.6 0 64 197 85 621.6 0 85 198 194 621.6 0 194 199 105 621.6 0 105 200 117 621.6 0 117 201 55 621.6 0 55 202 44 621.6 0 44 203 62 621.6 0 62 204 94 621.6 0 94 205 64 621.6 0 64 206 60 621.6 0 60 207 63 621.6 0 63 208 54 621.6 0 54 209 33 621.6 0 33 210 42 621.6 0 42 211 32 621.6 0 32 212 56 621.6 0 56 213 51 621.6 0 51 214 47 621.6 0 47 215 47 621.6 0 47 216 38 621.6 0 38 217 54 621.6 0 54 218 45 621.6 0 45 219 49 621.6 0 49 220 38 621.6 0 38 221 40 621.6 0 40 222 33 621.6 0 33 223 46 621.6 0 46 224 42 621.6 0 42 225 101 621.6 0 101 226 91 621.6 0 91 227 103 621.6 0 103 228 126 621.6 0 126 229 95 621.6 0 95 230 198 621.6 0 198 231 213 621.6 0 213 232 141 621.6 0 141 233 149 621.6 0 149 234 172 621.6 0 172 235 544 621.6 0 544 236 765 621.6 0 765 237 728 621.6 0 728 238 682 621.6 0 682 239 196 621.6 0 196 240 647 621.6 0 647 241 510 621.6 0 510 242 1437 621.6 0 1437 243 175 621.6 0 175 244 123 621.6 0 123 245 90 621.6 0 90 246 131 621.6 0 131 247 312 621.6 0 312 248 211 621.6 0 211 249 329 621.6 0 329 250 283 621.6 0 283 251 260 621.6 0 260 252 382 621.6 0 382 253 150 621.6 0 150 254 103 621.6 0 103 255 147 621.6 0 147 256 226 621.6 0 226 257 269 621.6 0 269 258 467 621.6 0 467 259 1106 621.6 0 1106 260 7690 621.6 0 7690 261 7689 621.6 0 7689 262 16578 621.6 0 16578 263 4145 621.6 0 4145 264 107 621.6 0 107 265 74 621.6 0 74 266 93 621.6 0 93 267 153 621.6 0 153 268 153 621.6 0 153 269 128 621.6 0 128 270 172 621.6 0 172 271 200 621.6 0 200 272 311 621.6 0 311 273 79 621.6 0 79 274 66 621.6 0 66 275 67 621.6 0 67 276 66 621.6 0 66 277 126 621.6 0 126 278 109 621.6 0 109 279 192 621.6 0 192 280 292 621.6 0 292 281 326 621.6 0 326 282 348 621.6 0 348 283 206 621.6 0 206 284 325 621.6 0 325 285 117 621.6 0 117 286 149 621.6 0 149 287 144 621.6 0 144 288 400 621.6 0 400 289 256 621.6 0 256 290 325 621.6 0 325 291 501 621.6 0 501 292 587 621.6 0 587 293 1025 621.6 0 1025 294 642 621.6 0 642 295 480 621.6 0 480 296 511 621.6 0 511 297 124 621.6 0 124 298 78 621.6 0 78 299 89 621.6 0 89 300 67 621.6 0 67 301 78 621.6 0 78 Finished in 3.87 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 77,705 Reads with adapters: 21,487 (27.7%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 21,461 (27.6%) Total basepairs processed: 19,026,838 bp Total written (filtered): 5,829,686 bp (30.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21487 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 18 0.0 3 0 0 11 3 4 19 351 0.0 3 0 312 22 8 9 20 20879 0.0 4 19469 1122 199 64 25 21 34 0.0 4 3 29 1 0 1 22 4 0.0 4 1 0 2 0 1 23 85 0.0 4 24 0 1 46 14 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 1 31 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 77 0.0 4 0 0 0 0 77 134 1 0.0 4 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 198 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.78 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 21,461 Reads with adapters: 5,276 (24.6%) Reads written (passing filters): 21,461 (100.0%) Total basepairs processed: 5,829,686 bp Total written (filtered): 5,771,559 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5276 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 8.8% G: 86.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 335.3 0 8 4 3 83.8 0 3 6 1 5.2 0 1 7 1 1.3 0 1 8 2 0.3 0 2 9 1043 0.1 0 118 925 10 3475 0.0 1 237 3238 11 686 0.0 1 25 661 12 17 0.0 1 1 16 13 6 0.0 1 0 6 14 1 0.0 1 0 1 15 1 0.0 1 0 1 18 1 0.0 1 0 1 40 1 0.0 1 1 150 3 0.0 1 1 2 169 1 0.0 1 1 189 1 0.0 1 0 1 208 23 0.0 1 16 7 209 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.