This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.28 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 97,550 (3.8%) Reads that were too short: 23,225 (0.9%) Reads written (passing filters): 74,325 (2.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 18,702,432 bp (2.4%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 97550 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 310 621.6 0 310 7 1902 621.6 0 1902 8 21442 621.6 0 21442 9 26104 621.6 0 26104 10 37 621.6 0 37 11 19 621.6 0 19 12 15 621.6 0 15 13 16 621.6 0 16 14 22 621.6 0 22 15 14 621.6 0 14 16 14 621.6 0 14 17 20 621.6 0 20 18 7 621.6 0 7 19 14 621.6 0 14 20 12 621.6 0 12 21 14 621.6 0 14 22 8 621.6 0 8 23 13 621.6 0 13 24 19 621.6 0 19 25 18 621.6 0 18 26 13 621.6 0 13 27 13 621.6 0 13 28 20 621.6 0 20 29 22 621.6 0 22 30 18 621.6 0 18 31 17 621.6 0 17 32 23 621.6 0 23 33 25 621.6 0 25 34 29 621.6 0 29 35 32 621.6 0 32 36 30 621.6 0 30 37 156 621.6 0 156 38 77 621.6 0 77 39 132 621.6 0 132 40 22 621.6 0 22 41 14 621.6 0 14 42 28 621.6 0 28 43 17 621.6 0 17 44 18 621.6 0 18 45 23 621.6 0 23 46 20 621.6 0 20 47 11 621.6 0 11 48 24 621.6 0 24 49 25 621.6 0 25 50 31 621.6 0 31 51 18 621.6 0 18 52 146 621.6 0 146 53 83 621.6 0 83 54 132 621.6 0 132 55 18 621.6 0 18 56 11 621.6 0 11 57 16 621.6 0 16 58 12 621.6 0 12 59 17 621.6 0 17 60 29 621.6 0 29 61 38 621.6 0 38 62 940 621.6 0 940 63 99 621.6 0 99 64 18 621.6 0 18 65 22 621.6 0 22 66 14 621.6 0 14 67 33 621.6 0 33 68 23 621.6 0 23 69 12 621.6 0 12 70 20 621.6 0 20 71 27 621.6 0 27 72 44 621.6 0 44 73 62 621.6 0 62 74 9 621.6 0 9 75 19 621.6 0 19 76 22 621.6 0 22 77 16 621.6 0 16 78 20 621.6 0 20 79 25 621.6 0 25 80 12 621.6 0 12 81 51 621.6 0 51 82 18 621.6 0 18 83 21 621.6 0 21 84 81 621.6 0 81 85 23 621.6 0 23 86 80 621.6 0 80 87 22 621.6 0 22 88 14 621.6 0 14 89 33 621.6 0 33 90 35 621.6 0 35 91 26 621.6 0 26 92 39 621.6 0 39 93 33 621.6 0 33 94 31 621.6 0 31 95 51 621.6 0 51 96 35 621.6 0 35 97 56 621.6 0 56 98 16 621.6 0 16 99 17 621.6 0 17 100 19 621.6 0 19 101 20 621.6 0 20 102 14 621.6 0 14 103 29 621.6 0 29 104 81 621.6 0 81 105 67 621.6 0 67 106 99 621.6 0 99 107 46 621.6 0 46 108 103 621.6 0 103 109 66 621.6 0 66 110 125 621.6 0 125 111 60 621.6 0 60 112 80 621.6 0 80 113 72 621.6 0 72 114 64 621.6 0 64 115 43 621.6 0 43 116 30 621.6 0 30 117 58 621.6 0 58 118 50 621.6 0 50 119 40 621.6 0 40 120 32 621.6 0 32 121 14 621.6 0 14 122 22 621.6 0 22 123 22 621.6 0 22 124 27 621.6 0 27 125 47 621.6 0 47 126 88 621.6 0 88 127 283 621.6 0 283 128 88 621.6 0 88 129 191 621.6 0 191 130 35 621.6 0 35 131 50 621.6 0 50 132 88 621.6 0 88 133 38 621.6 0 38 134 34 621.6 0 34 135 24 621.6 0 24 136 37 621.6 0 37 137 39 621.6 0 39 138 49 621.6 0 49 139 79 621.6 0 79 140 112 621.6 0 112 141 102 621.6 0 102 142 222 621.6 0 222 143 527 621.6 0 527 144 6256 621.6 0 6256 145 3043 621.6 0 3043 146 5259 621.6 0 5259 147 39 621.6 0 39 148 26 621.6 0 26 149 48 621.6 0 48 150 33 621.6 0 33 151 91 621.6 0 91 152 58 621.6 0 58 153 115 621.6 0 115 154 53 621.6 0 53 155 26 621.6 0 26 156 78 621.6 0 78 157 61 621.6 0 61 158 628 621.6 0 628 159 32 621.6 0 32 160 23 621.6 0 23 161 26 621.6 0 26 162 26 621.6 0 26 163 29 621.6 0 29 164 29 621.6 0 29 165 21 621.6 0 21 166 21 621.6 0 21 167 23 621.6 0 23 168 19 621.6 0 19 169 14 621.6 0 14 170 21 621.6 0 21 171 30 621.6 0 30 172 41 621.6 0 41 173 28 621.6 0 28 174 27 621.6 0 27 175 17 621.6 0 17 176 21 621.6 0 21 177 25 621.6 0 25 178 26 621.6 0 26 179 91 621.6 0 91 180 58 621.6 0 58 181 41 621.6 0 41 182 31 621.6 0 31 183 24 621.6 0 24 184 22 621.6 0 22 185 32 621.6 0 32 186 48 621.6 0 48 187 37 621.6 0 37 188 23 621.6 0 23 189 24 621.6 0 24 190 57 621.6 0 57 191 113 621.6 0 113 192 212 621.6 0 212 193 27 621.6 0 27 194 24 621.6 0 24 195 18 621.6 0 18 196 33 621.6 0 33 197 33 621.6 0 33 198 73 621.6 0 73 199 22 621.6 0 22 200 39 621.6 0 39 201 15 621.6 0 15 202 13 621.6 0 13 203 23 621.6 0 23 204 42 621.6 0 42 205 23 621.6 0 23 206 28 621.6 0 28 207 25 621.6 0 25 208 11 621.6 0 11 209 24 621.6 0 24 210 14 621.6 0 14 211 5 621.6 0 5 212 14 621.6 0 14 213 23 621.6 0 23 214 27 621.6 0 27 215 27 621.6 0 27 216 14 621.6 0 14 217 27 621.6 0 27 218 14 621.6 0 14 219 36 621.6 0 36 220 23 621.6 0 23 221 19 621.6 0 19 222 21 621.6 0 21 223 18 621.6 0 18 224 36 621.6 0 36 225 36 621.6 0 36 226 30 621.6 0 30 227 38 621.6 0 38 228 34 621.6 0 34 229 51 621.6 0 51 230 101 621.6 0 101 231 147 621.6 0 147 232 84 621.6 0 84 233 53 621.6 0 53 234 70 621.6 0 70 235 182 621.6 0 182 236 247 621.6 0 247 237 240 621.6 0 240 238 189 621.6 0 189 239 65 621.6 0 65 240 243 621.6 0 243 241 172 621.6 0 172 242 373 621.6 0 373 243 64 621.6 0 64 244 47 621.6 0 47 245 36 621.6 0 36 246 54 621.6 0 54 247 107 621.6 0 107 248 65 621.6 0 65 249 129 621.6 0 129 250 110 621.6 0 110 251 129 621.6 0 129 252 170 621.6 0 170 253 61 621.6 0 61 254 33 621.6 0 33 255 53 621.6 0 53 256 75 621.6 0 75 257 79 621.6 0 79 258 165 621.6 0 165 259 428 621.6 0 428 260 3607 621.6 0 3607 261 3238 621.6 0 3238 262 6473 621.6 0 6473 263 1871 621.6 0 1871 264 67 621.6 0 67 265 28 621.6 0 28 266 37 621.6 0 37 267 54 621.6 0 54 268 59 621.6 0 59 269 44 621.6 0 44 270 89 621.6 0 89 271 52 621.6 0 52 272 180 621.6 0 180 273 40 621.6 0 40 274 31 621.6 0 31 275 27 621.6 0 27 276 37 621.6 0 37 277 64 621.6 0 64 278 54 621.6 0 54 279 78 621.6 0 78 280 87 621.6 0 87 281 93 621.6 0 93 282 86 621.6 0 86 283 184 621.6 0 184 284 379 621.6 0 379 285 97 621.6 0 97 286 49 621.6 0 49 287 46 621.6 0 46 288 177 621.6 0 177 289 112 621.6 0 112 290 91 621.6 0 91 291 145 621.6 0 145 292 131 621.6 0 131 293 224 621.6 0 224 294 122 621.6 0 122 295 94 621.6 0 94 296 108 621.6 0 108 297 50 621.6 0 50 298 36 621.6 0 36 299 40 621.6 0 40 300 27 621.6 0 27 301 50 621.6 0 50 Finished in 3.34 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 74,325 Reads with adapters: 22,333 (30.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 22,319 (30.0%) Total basepairs processed: 18,702,432 bp Total written (filtered): 6,160,142 bp (32.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22333 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 131 0.0 3 0 130 1 17 22086 0.0 3 21696 378 10 2 18 54 0.0 3 3 48 2 1 19 9 0.0 3 0 1 6 2 20 28 0.0 3 0 0 0 28 43 1 0.0 3 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 233 1 0.0 3 1 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 22,319 Reads with adapters: 160 (0.7%) Reads written (passing filters): 22,319 (100.0%) Total basepairs processed: 6,160,142 bp Total written (filtered): 6,159,565 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 160 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.6% C: 7.5% G: 54.4% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 64 348.7 0 64 4 95 87.2 0 95 5 1 21.8 0 1