This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.70 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 135,981 (5.3%) Reads that were too short: 17,442 (0.7%) Reads written (passing filters): 118,539 (4.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,702,202 bp (4.1%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 135981 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 970 621.6 0 970 7 48300 621.6 0 48300 8 40702 621.6 0 40702 9 103 621.6 0 103 10 55 621.6 0 55 11 73 621.6 0 73 12 224 621.6 0 224 13 50 621.6 0 50 14 43 621.6 0 43 15 42 621.6 0 42 16 47 621.6 0 47 17 52 621.6 0 52 18 81 621.6 0 81 19 75 621.6 0 75 20 154 621.6 0 154 21 71 621.6 0 71 22 72 621.6 0 72 23 56 621.6 0 56 24 66 621.6 0 66 25 61 621.6 0 61 26 87 621.6 0 87 27 67 621.6 0 67 28 66 621.6 0 66 29 62 621.6 0 62 30 58 621.6 0 58 31 55 621.6 0 55 32 69 621.6 0 69 33 49 621.6 0 49 34 93 621.6 0 93 35 68 621.6 0 68 36 52 621.6 0 52 37 62 621.6 0 62 38 60 621.6 0 60 39 81 621.6 0 81 40 66 621.6 0 66 41 56 621.6 0 56 42 48 621.6 0 48 43 65 621.6 0 65 44 73 621.6 0 73 45 68 621.6 0 68 46 71 621.6 0 71 47 95 621.6 0 95 48 68 621.6 0 68 49 52 621.6 0 52 50 102 621.6 0 102 51 47 621.6 0 47 52 43 621.6 0 43 53 72 621.6 0 72 54 99 621.6 0 99 55 82 621.6 0 82 56 56 621.6 0 56 57 56 621.6 0 56 58 73 621.6 0 73 59 89 621.6 0 89 60 61 621.6 0 61 61 64 621.6 0 64 62 63 621.6 0 63 63 65 621.6 0 65 64 59 621.6 0 59 65 69 621.6 0 69 66 98 621.6 0 98 67 113 621.6 0 113 68 113 621.6 0 113 69 154 621.6 0 154 70 230 621.6 0 230 71 1943 621.6 0 1943 72 1821 621.6 0 1821 73 1434 621.6 0 1434 74 72 621.6 0 72 75 43 621.6 0 43 76 74 621.6 0 74 77 127 621.6 0 127 78 78 621.6 0 78 79 168 621.6 0 168 80 59 621.6 0 59 81 49 621.6 0 49 82 77 621.6 0 77 83 86 621.6 0 86 84 84 621.6 0 84 85 79 621.6 0 79 86 101 621.6 0 101 87 124 621.6 0 124 88 69 621.6 0 69 89 59 621.6 0 59 90 117 621.6 0 117 91 77 621.6 0 77 92 65 621.6 0 65 93 114 621.6 0 114 94 122 621.6 0 122 95 173 621.6 0 173 96 881 621.6 0 881 97 371 621.6 0 371 98 748 621.6 0 748 99 161 621.6 0 161 100 69 621.6 0 69 101 65 621.6 0 65 102 49 621.6 0 49 103 72 621.6 0 72 104 51 621.6 0 51 105 58 621.6 0 58 106 66 621.6 0 66 107 60 621.6 0 60 108 77 621.6 0 77 109 80 621.6 0 80 110 52 621.6 0 52 111 66 621.6 0 66 112 80 621.6 0 80 113 81 621.6 0 81 114 84 621.6 0 84 115 78 621.6 0 78 116 85 621.6 0 85 117 88 621.6 0 88 118 92 621.6 0 92 119 96 621.6 0 96 120 73 621.6 0 73 121 75 621.6 0 75 122 70 621.6 0 70 123 73 621.6 0 73 124 127 621.6 0 127 125 191 621.6 0 191 126 218 621.6 0 218 127 135 621.6 0 135 128 158 621.6 0 158 129 174 621.6 0 174 130 171 621.6 0 171 131 107 621.6 0 107 132 106 621.6 0 106 133 155 621.6 0 155 134 100 621.6 0 100 135 177 621.6 0 177 136 112 621.6 0 112 137 106 621.6 0 106 138 105 621.6 0 105 139 337 621.6 0 337 140 96 621.6 0 96 141 108 621.6 0 108 142 100 621.6 0 100 143 117 621.6 0 117 144 76 621.6 0 76 145 92 621.6 0 92 146 93 621.6 0 93 147 70 621.6 0 70 148 81 621.6 0 81 149 72 621.6 0 72 150 72 621.6 0 72 151 107 621.6 0 107 152 78 621.6 0 78 153 114 621.6 0 114 154 117 621.6 0 117 155 119 621.6 0 119 156 104 621.6 0 104 157 74 621.6 0 74 158 73 621.6 0 73 159 88 621.6 0 88 160 90 621.6 0 90 161 79 621.6 0 79 162 77 621.6 0 77 163 65 621.6 0 65 164 95 621.6 0 95 165 107 621.6 0 107 166 97 621.6 0 97 167 92 621.6 0 92 168 80 621.6 0 80 169 80 621.6 0 80 170 101 621.6 0 101 171 93 621.6 0 93 172 140 621.6 0 140 173 365 621.6 0 365 174 189 621.6 0 189 175 330 621.6 0 330 176 122 621.6 0 122 177 77 621.6 0 77 178 75 621.6 0 75 179 105 621.6 0 105 180 120 621.6 0 120 181 91 621.6 0 91 182 78 621.6 0 78 183 82 621.6 0 82 184 104 621.6 0 104 185 115 621.6 0 115 186 146 621.6 0 146 187 133 621.6 0 133 188 83 621.6 0 83 189 95 621.6 0 95 190 142 621.6 0 142 191 233 621.6 0 233 192 656 621.6 0 656 193 883 621.6 0 883 194 1737 621.6 0 1737 195 710 621.6 0 710 196 77 621.6 0 77 197 61 621.6 0 61 198 103 621.6 0 103 199 98 621.6 0 98 200 97 621.6 0 97 201 94 621.6 0 94 202 71 621.6 0 71 203 112 621.6 0 112 204 96 621.6 0 96 205 85 621.6 0 85 206 118 621.6 0 118 207 194 621.6 0 194 208 110 621.6 0 110 209 152 621.6 0 152 210 191 621.6 0 191 211 181 621.6 0 181 212 156 621.6 0 156 213 94 621.6 0 94 214 97 621.6 0 97 215 193 621.6 0 193 216 313 621.6 0 313 217 289 621.6 0 289 218 88 621.6 0 88 219 130 621.6 0 130 220 145 621.6 0 145 221 136 621.6 0 136 222 91 621.6 0 91 223 78 621.6 0 78 224 78 621.6 0 78 225 108 621.6 0 108 226 133 621.6 0 133 227 124 621.6 0 124 228 84 621.6 0 84 229 90 621.6 0 90 230 101 621.6 0 101 231 96 621.6 0 96 232 97 621.6 0 97 233 113 621.6 0 113 234 88 621.6 0 88 235 107 621.6 0 107 236 134 621.6 0 134 237 144 621.6 0 144 238 124 621.6 0 124 239 82 621.6 0 82 240 100 621.6 0 100 241 114 621.6 0 114 242 115 621.6 0 115 243 91 621.6 0 91 244 84 621.6 0 84 245 107 621.6 0 107 246 115 621.6 0 115 247 127 621.6 0 127 248 113 621.6 0 113 249 154 621.6 0 154 250 101 621.6 0 101 251 86 621.6 0 86 252 83 621.6 0 83 253 142 621.6 0 142 254 181 621.6 0 181 255 199 621.6 0 199 256 137 621.6 0 137 257 116 621.6 0 116 258 155 621.6 0 155 259 177 621.6 0 177 260 148 621.6 0 148 261 114 621.6 0 114 262 211 621.6 0 211 263 114 621.6 0 114 264 108 621.6 0 108 265 95 621.6 0 95 266 136 621.6 0 136 267 199 621.6 0 199 268 154 621.6 0 154 269 138 621.6 0 138 270 194 621.6 0 194 271 227 621.6 0 227 272 2153 621.6 0 2153 273 953 621.6 0 953 274 1284 621.6 0 1284 275 171 621.6 0 171 276 103 621.6 0 103 277 81 621.6 0 81 278 151 621.6 0 151 279 223 621.6 0 223 280 204 621.6 0 204 281 151 621.6 0 151 282 137 621.6 0 137 283 87 621.6 0 87 284 121 621.6 0 121 285 119 621.6 0 119 286 144 621.6 0 144 287 114 621.6 0 114 288 132 621.6 0 132 289 139 621.6 0 139 290 163 621.6 0 163 291 146 621.6 0 146 292 140 621.6 0 140 293 137 621.6 0 137 294 226 621.6 0 226 295 195 621.6 0 195 296 227 621.6 0 227 297 129 621.6 0 129 298 90 621.6 0 90 299 123 621.6 0 123 300 138 621.6 0 138 301 103 621.6 0 103 Finished in 6.14 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 118,539 Reads with adapters: 42,145 (35.6%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 42,101 (35.5%) Total basepairs processed: 31,702,202 bp Total written (filtered): 11,482,624 bp (36.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42145 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 4 4 18 23 0.0 3 0 0 13 9 1 19 765 0.0 3 0 640 65 42 18 20 40902 0.0 4 38015 2258 453 136 40 21 100 0.0 4 17 69 7 3 4 22 13 0.0 4 1 0 10 1 1 23 174 0.0 4 0 0 1 109 64 24 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 53 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 1 127 100 0.0 4 0 0 0 0 100 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 172 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 1 209 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 1 219 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.51 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 42,101 Reads with adapters: 8,125 (19.3%) Reads written (passing filters): 42,101 (100.0%) Total basepairs processed: 11,482,624 bp Total written (filtered): 11,387,771 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8125 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 9.1% G: 85.8% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 101 657.8 0 101 4 17 164.5 0 17 7 1 2.6 0 1 8 1 0.6 0 1 9 139 0.2 0 13 126 10 652 0.0 1 46 606 11 7099 0.0 1 212 6887 12 66 0.0 1 1 65 13 3 0.0 1 0 3 14 1 0.0 1 0 1 15 3 0.0 1 0 3 150 1 0.0 1 0 1 151 16 0.0 1 13 3 163 1 0.0 1 1 178 1 0.0 1 0 1 190 1 0.0 1 0 1 209 19 0.0 1 15 4 210 2 0.0 1 1 1 229 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.