This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.84 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 125,972 (4.9%) Reads that were too short: 8,875 (0.3%) Reads written (passing filters): 117,097 (4.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 32,127,666 bp (4.2%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 125972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 799 621.6 0 799 7 44912 621.6 0 44912 8 48406 621.6 0 48406 9 75 621.6 0 75 10 58 621.6 0 58 11 47 621.6 0 47 12 124 621.6 0 124 13 62 621.6 0 62 14 43 621.6 0 43 15 49 621.6 0 49 16 39 621.6 0 39 17 47 621.6 0 47 18 87 621.6 0 87 19 59 621.6 0 59 20 48 621.6 0 48 21 52 621.6 0 52 22 67 621.6 0 67 23 54 621.6 0 54 24 62 621.6 0 62 25 48 621.6 0 48 26 73 621.6 0 73 27 45 621.6 0 45 28 47 621.6 0 47 29 38 621.6 0 38 30 64 621.6 0 64 31 64 621.6 0 64 32 55 621.6 0 55 33 40 621.6 0 40 34 62 621.6 0 62 35 56 621.6 0 56 36 85 621.6 0 85 37 73 621.6 0 73 38 60 621.6 0 60 39 71 621.6 0 71 40 71 621.6 0 71 41 70 621.6 0 70 42 58 621.6 0 58 43 62 621.6 0 62 44 69 621.6 0 69 45 47 621.6 0 47 46 61 621.6 0 61 47 69 621.6 0 69 48 52 621.6 0 52 49 47 621.6 0 47 50 130 621.6 0 130 51 69 621.6 0 69 52 65 621.6 0 65 53 67 621.6 0 67 54 89 621.6 0 89 55 67 621.6 0 67 56 64 621.6 0 64 57 65 621.6 0 65 58 63 621.6 0 63 59 57 621.6 0 57 60 55 621.6 0 55 61 50 621.6 0 50 62 47 621.6 0 47 63 66 621.6 0 66 64 63 621.6 0 63 65 65 621.6 0 65 66 79 621.6 0 79 67 108 621.6 0 108 68 105 621.6 0 105 69 147 621.6 0 147 70 200 621.6 0 200 71 2245 621.6 0 2245 72 2205 621.6 0 2205 73 1662 621.6 0 1662 74 68 621.6 0 68 75 47 621.6 0 47 76 45 621.6 0 45 77 61 621.6 0 61 78 56 621.6 0 56 79 113 621.6 0 113 80 53 621.6 0 53 81 58 621.6 0 58 82 51 621.6 0 51 83 57 621.6 0 57 84 53 621.6 0 53 85 54 621.6 0 54 86 58 621.6 0 58 87 57 621.6 0 57 88 52 621.6 0 52 89 52 621.6 0 52 90 107 621.6 0 107 91 71 621.6 0 71 92 63 621.6 0 63 93 114 621.6 0 114 94 81 621.6 0 81 95 159 621.6 0 159 96 499 621.6 0 499 97 438 621.6 0 438 98 794 621.6 0 794 99 171 621.6 0 171 100 63 621.6 0 63 101 50 621.6 0 50 102 44 621.6 0 44 103 56 621.6 0 56 104 54 621.6 0 54 105 49 621.6 0 49 106 62 621.6 0 62 107 51 621.6 0 51 108 55 621.6 0 55 109 74 621.6 0 74 110 55 621.6 0 55 111 40 621.6 0 40 112 83 621.6 0 83 113 75 621.6 0 75 114 69 621.6 0 69 115 77 621.6 0 77 116 60 621.6 0 60 117 75 621.6 0 75 118 86 621.6 0 86 119 71 621.6 0 71 120 53 621.6 0 53 121 59 621.6 0 59 122 50 621.6 0 50 123 57 621.6 0 57 124 94 621.6 0 94 125 134 621.6 0 134 126 118 621.6 0 118 127 84 621.6 0 84 128 96 621.6 0 96 129 92 621.6 0 92 130 106 621.6 0 106 131 75 621.6 0 75 132 57 621.6 0 57 133 67 621.6 0 67 134 69 621.6 0 69 135 97 621.6 0 97 136 80 621.6 0 80 137 95 621.6 0 95 138 72 621.6 0 72 139 135 621.6 0 135 140 62 621.6 0 62 141 63 621.6 0 63 142 71 621.6 0 71 143 74 621.6 0 74 144 75 621.6 0 75 145 70 621.6 0 70 146 57 621.6 0 57 147 58 621.6 0 58 148 63 621.6 0 63 149 58 621.6 0 58 150 62 621.6 0 62 151 83 621.6 0 83 152 66 621.6 0 66 153 50 621.6 0 50 154 87 621.6 0 87 155 63 621.6 0 63 156 67 621.6 0 67 157 73 621.6 0 73 158 65 621.6 0 65 159 57 621.6 0 57 160 85 621.6 0 85 161 71 621.6 0 71 162 59 621.6 0 59 163 68 621.6 0 68 164 57 621.6 0 57 165 69 621.6 0 69 166 66 621.6 0 66 167 69 621.6 0 69 168 52 621.6 0 52 169 51 621.6 0 51 170 72 621.6 0 72 171 76 621.6 0 76 172 89 621.6 0 89 173 146 621.6 0 146 174 110 621.6 0 110 175 152 621.6 0 152 176 72 621.6 0 72 177 54 621.6 0 54 178 58 621.6 0 58 179 68 621.6 0 68 180 79 621.6 0 79 181 67 621.6 0 67 182 64 621.6 0 64 183 58 621.6 0 58 184 72 621.6 0 72 185 71 621.6 0 71 186 74 621.6 0 74 187 80 621.6 0 80 188 60 621.6 0 60 189 78 621.6 0 78 190 102 621.6 0 102 191 135 621.6 0 135 192 394 621.6 0 394 193 523 621.6 0 523 194 868 621.6 0 868 195 363 621.6 0 363 196 68 621.6 0 68 197 49 621.6 0 49 198 48 621.6 0 48 199 82 621.6 0 82 200 67 621.6 0 67 201 46 621.6 0 46 202 61 621.6 0 61 203 56 621.6 0 56 204 50 621.6 0 50 205 52 621.6 0 52 206 76 621.6 0 76 207 96 621.6 0 96 208 68 621.6 0 68 209 57 621.6 0 57 210 75 621.6 0 75 211 85 621.6 0 85 212 57 621.6 0 57 213 62 621.6 0 62 214 64 621.6 0 64 215 72 621.6 0 72 216 82 621.6 0 82 217 121 621.6 0 121 218 53 621.6 0 53 219 65 621.6 0 65 220 41 621.6 0 41 221 48 621.6 0 48 222 48 621.6 0 48 223 56 621.6 0 56 224 56 621.6 0 56 225 104 621.6 0 104 226 78 621.6 0 78 227 98 621.6 0 98 228 52 621.6 0 52 229 87 621.6 0 87 230 64 621.6 0 64 231 43 621.6 0 43 232 54 621.6 0 54 233 83 621.6 0 83 234 51 621.6 0 51 235 75 621.6 0 75 236 82 621.6 0 82 237 61 621.6 0 61 238 74 621.6 0 74 239 66 621.6 0 66 240 49 621.6 0 49 241 54 621.6 0 54 242 54 621.6 0 54 243 63 621.6 0 63 244 67 621.6 0 67 245 43 621.6 0 43 246 62 621.6 0 62 247 65 621.6 0 65 248 73 621.6 0 73 249 91 621.6 0 91 250 75 621.6 0 75 251 70 621.6 0 70 252 57 621.6 0 57 253 83 621.6 0 83 254 107 621.6 0 107 255 84 621.6 0 84 256 64 621.6 0 64 257 94 621.6 0 94 258 82 621.6 0 82 259 74 621.6 0 74 260 80 621.6 0 80 261 65 621.6 0 65 262 96 621.6 0 96 263 79 621.6 0 79 264 56 621.6 0 56 265 64 621.6 0 64 266 85 621.6 0 85 267 82 621.6 0 82 268 80 621.6 0 80 269 86 621.6 0 86 270 116 621.6 0 116 271 100 621.6 0 100 272 812 621.6 0 812 273 388 621.6 0 388 274 451 621.6 0 451 275 82 621.6 0 82 276 64 621.6 0 64 277 59 621.6 0 59 278 136 621.6 0 136 279 105 621.6 0 105 280 108 621.6 0 108 281 84 621.6 0 84 282 66 621.6 0 66 283 64 621.6 0 64 284 79 621.6 0 79 285 70 621.6 0 70 286 72 621.6 0 72 287 69 621.6 0 69 288 72 621.6 0 72 289 91 621.6 0 91 290 113 621.6 0 113 291 89 621.6 0 89 292 81 621.6 0 81 293 66 621.6 0 66 294 118 621.6 0 118 295 81 621.6 0 81 296 94 621.6 0 94 297 71 621.6 0 71 298 71 621.6 0 71 299 64 621.6 0 64 300 103 621.6 0 103 301 79 621.6 0 79 Finished in 5.47 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 117,097 Reads with adapters: 44,101 (37.7%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 44,082 (37.6%) Total basepairs processed: 32,127,666 bp Total written (filtered): 12,208,512 bp (38.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 44101 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 8 1 16 221 0.0 3 0 216 5 17 43742 0.0 3 42934 794 11 3 18 61 0.0 3 3 54 2 2 19 8 0.0 3 0 0 6 2 20 24 0.0 3 1 0 0 23 27 1 0.0 3 1 29 1 0.0 3 1 31 1 0.0 3 1 40 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 1 1 68 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 5 0.0 3 0 0 0 5 213 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.57 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 44,082 Reads with adapters: 64 (0.1%) Reads written (passing filters): 44,082 (100.0%) Total basepairs processed: 12,208,512 bp Total written (filtered): 12,208,307 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 64 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.2% C: 12.5% G: 40.6% T: 15.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 51 688.8 0 51 4 13 172.2 0 13