This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.38 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 203,774 (8.0%) Reads that were too short: 7,691 (0.3%) Reads written (passing filters): 196,083 (7.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 54,512,807 bp (7.1%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 203774 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1699 621.6 0 1699 7 79283 621.6 0 79283 8 2334 621.6 0 2334 9 81793 621.6 0 81793 10 116 621.6 0 116 11 94 621.6 0 94 12 80 621.6 0 80 13 56 621.6 0 56 14 79 621.6 0 79 15 71 621.6 0 71 16 206 621.6 0 206 17 146 621.6 0 146 18 189 621.6 0 189 19 79 621.6 0 79 20 73 621.6 0 73 21 76 621.6 0 76 22 106 621.6 0 106 23 74 621.6 0 74 24 70 621.6 0 70 25 60 621.6 0 60 26 48 621.6 0 48 27 51 621.6 0 51 28 56 621.6 0 56 29 48 621.6 0 48 30 62 621.6 0 62 31 59 621.6 0 59 32 72 621.6 0 72 33 75 621.6 0 75 34 73 621.6 0 73 35 70 621.6 0 70 36 72 621.6 0 72 37 100 621.6 0 100 38 69 621.6 0 69 39 72 621.6 0 72 40 51 621.6 0 51 41 83 621.6 0 83 42 81 621.6 0 81 43 77 621.6 0 77 44 75 621.6 0 75 45 80 621.6 0 80 46 203 621.6 0 203 47 197 621.6 0 197 48 353 621.6 0 353 49 78 621.6 0 78 50 81 621.6 0 81 51 76 621.6 0 76 52 92 621.6 0 92 53 65 621.6 0 65 54 55 621.6 0 55 55 61 621.6 0 61 56 66 621.6 0 66 57 71 621.6 0 71 58 65 621.6 0 65 59 85 621.6 0 85 60 81 621.6 0 81 61 67 621.6 0 67 62 84 621.6 0 84 63 92 621.6 0 92 64 73 621.6 0 73 65 77 621.6 0 77 66 77 621.6 0 77 67 105 621.6 0 105 68 85 621.6 0 85 69 68 621.6 0 68 70 85 621.6 0 85 71 92 621.6 0 92 72 62 621.6 0 62 73 78 621.6 0 78 74 87 621.6 0 87 75 77 621.6 0 77 76 91 621.6 0 91 77 85 621.6 0 85 78 73 621.6 0 73 79 205 621.6 0 205 80 193 621.6 0 193 81 361 621.6 0 361 82 100 621.6 0 100 83 318 621.6 0 318 84 179 621.6 0 179 85 119 621.6 0 119 86 98 621.6 0 98 87 129 621.6 0 129 88 135 621.6 0 135 89 127 621.6 0 127 90 124 621.6 0 124 91 142 621.6 0 142 92 195 621.6 0 195 93 284 621.6 0 284 94 4604 621.6 0 4604 95 1495 621.6 0 1495 96 3117 621.6 0 3117 97 657 621.6 0 657 98 818 621.6 0 818 99 73 621.6 0 73 100 60 621.6 0 60 101 78 621.6 0 78 102 64 621.6 0 64 103 56 621.6 0 56 104 62 621.6 0 62 105 80 621.6 0 80 106 60 621.6 0 60 107 74 621.6 0 74 108 60 621.6 0 60 109 77 621.6 0 77 110 64 621.6 0 64 111 66 621.6 0 66 112 92 621.6 0 92 113 61 621.6 0 61 114 72 621.6 0 72 115 103 621.6 0 103 116 81 621.6 0 81 117 85 621.6 0 85 118 146 621.6 0 146 119 81 621.6 0 81 120 113 621.6 0 113 121 75 621.6 0 75 122 65 621.6 0 65 123 67 621.6 0 67 124 121 621.6 0 121 125 117 621.6 0 117 126 121 621.6 0 121 127 102 621.6 0 102 128 74 621.6 0 74 129 121 621.6 0 121 130 141 621.6 0 141 131 1216 621.6 0 1216 132 432 621.6 0 432 133 588 621.6 0 588 134 62 621.6 0 62 135 87 621.6 0 87 136 80 621.6 0 80 137 188 621.6 0 188 138 203 621.6 0 203 139 329 621.6 0 329 140 78 621.6 0 78 141 123 621.6 0 123 142 115 621.6 0 115 143 144 621.6 0 144 144 253 621.6 0 253 145 162 621.6 0 162 146 173 621.6 0 173 147 290 621.6 0 290 148 124 621.6 0 124 149 129 621.6 0 129 150 81 621.6 0 81 151 72 621.6 0 72 152 56 621.6 0 56 153 60 621.6 0 60 154 56 621.6 0 56 155 45 621.6 0 45 156 71 621.6 0 71 157 104 621.6 0 104 158 170 621.6 0 170 159 127 621.6 0 127 160 282 621.6 0 282 161 70 621.6 0 70 162 57 621.6 0 57 163 63 621.6 0 63 164 60 621.6 0 60 165 69 621.6 0 69 166 81 621.6 0 81 167 72 621.6 0 72 168 80 621.6 0 80 169 60 621.6 0 60 170 51 621.6 0 51 171 73 621.6 0 73 172 81 621.6 0 81 173 69 621.6 0 69 174 61 621.6 0 61 175 59 621.6 0 59 176 56 621.6 0 56 177 61 621.6 0 61 178 73 621.6 0 73 179 60 621.6 0 60 180 101 621.6 0 101 181 66 621.6 0 66 182 83 621.6 0 83 183 63 621.6 0 63 184 91 621.6 0 91 185 82 621.6 0 82 186 73 621.6 0 73 187 116 621.6 0 116 188 66 621.6 0 66 189 54 621.6 0 54 190 73 621.6 0 73 191 76 621.6 0 76 192 150 621.6 0 150 193 89 621.6 0 89 194 95 621.6 0 95 195 68 621.6 0 68 196 48 621.6 0 48 197 58 621.6 0 58 198 60 621.6 0 60 199 47 621.6 0 47 200 58 621.6 0 58 201 53 621.6 0 53 202 74 621.6 0 74 203 70 621.6 0 70 204 71 621.6 0 71 205 162 621.6 0 162 206 120 621.6 0 120 207 157 621.6 0 157 208 56 621.6 0 56 209 70 621.6 0 70 210 81 621.6 0 81 211 61 621.6 0 61 212 57 621.6 0 57 213 53 621.6 0 53 214 58 621.6 0 58 215 59 621.6 0 59 216 60 621.6 0 60 217 61 621.6 0 61 218 51 621.6 0 51 219 61 621.6 0 61 220 55 621.6 0 55 221 44 621.6 0 44 222 44 621.6 0 44 223 51 621.6 0 51 224 62 621.6 0 62 225 36 621.6 0 36 226 35 621.6 0 35 227 45 621.6 0 45 228 79 621.6 0 79 229 63 621.6 0 63 230 50 621.6 0 50 231 28 621.6 0 28 232 71 621.6 0 71 233 106 621.6 0 106 234 47 621.6 0 47 235 54 621.6 0 54 236 47 621.6 0 47 237 46 621.6 0 46 238 53 621.6 0 53 239 56 621.6 0 56 240 70 621.6 0 70 241 95 621.6 0 95 242 70 621.6 0 70 243 87 621.6 0 87 244 61 621.6 0 61 245 67 621.6 0 67 246 55 621.6 0 55 247 65 621.6 0 65 248 58 621.6 0 58 249 77 621.6 0 77 250 43 621.6 0 43 251 57 621.6 0 57 252 58 621.6 0 58 253 52 621.6 0 52 254 59 621.6 0 59 255 68 621.6 0 68 256 87 621.6 0 87 257 101 621.6 0 101 258 77 621.6 0 77 259 84 621.6 0 84 260 65 621.6 0 65 261 137 621.6 0 137 262 132 621.6 0 132 263 124 621.6 0 124 264 95 621.6 0 95 265 51 621.6 0 51 266 66 621.6 0 66 267 61 621.6 0 61 268 54 621.6 0 54 269 48 621.6 0 48 270 255 621.6 0 255 271 254 621.6 0 254 272 116 621.6 0 116 273 67 621.6 0 67 274 66 621.6 0 66 275 56 621.6 0 56 276 86 621.6 0 86 277 81 621.6 0 81 278 57 621.6 0 57 279 59 621.6 0 59 280 62 621.6 0 62 281 83 621.6 0 83 282 111 621.6 0 111 283 179 621.6 0 179 284 91 621.6 0 91 285 57 621.6 0 57 286 83 621.6 0 83 287 92 621.6 0 92 288 90 621.6 0 90 289 86 621.6 0 86 290 111 621.6 0 111 291 66 621.6 0 66 292 103 621.6 0 103 293 57 621.6 0 57 294 64 621.6 0 64 295 67 621.6 0 67 296 72 621.6 0 72 297 71 621.6 0 71 298 83 621.6 0 83 299 104 621.6 0 104 300 119 621.6 0 119 301 65 621.6 0 65 Finished in 9.97 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 196,083 Reads with adapters: 79,954 (40.8%) Reads that were too short: 149 (0.1%) Reads written (passing filters): 79,805 (40.7%) Total basepairs processed: 54,512,807 bp Total written (filtered): 21,808,769 bp (40.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79954 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 2 18 37 0.0 3 0 0 22 13 2 19 832 0.0 3 0 680 80 46 26 20 77938 0.0 4 72703 4117 834 219 65 21 212 0.0 4 33 157 14 5 3 22 27 0.0 4 1 1 19 4 2 23 204 0.0 4 0 1 0 151 52 24 5 0.0 4 3 0 0 0 2 25 2 0.0 4 2 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 29 3 0.0 4 2 1 30 1 0.0 4 0 0 1 33 2 0.0 4 0 0 1 0 1 34 1 0.0 4 1 39 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 109 1 0.0 4 1 115 5 0.0 4 0 0 0 0 5 116 1 0.0 4 0 0 0 0 1 117 4 0.0 4 0 0 0 0 4 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 509 0.0 4 0 0 0 0 509 128 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 141 64 0.0 4 0 0 0 4 60 142 11 0.0 4 0 0 0 1 10 143 4 0.0 4 0 0 0 0 4 149 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 189 2 0.0 4 0 0 0 0 2 191 1 0.0 4 0 0 1 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 229 17 0.0 4 0 0 0 0 17 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 238 2 0.0 4 0 0 0 0 2 249 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.75 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 79,805 Reads with adapters: 3,864 (4.8%) Reads written (passing filters): 79,805 (100.0%) Total basepairs processed: 21,808,769 bp Total written (filtered): 21,753,558 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3864 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.6% C: 15.6% G: 70.9% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 436 1247.0 0 436 4 51 311.7 0 51 5 5 77.9 0 5 6 1 19.5 0 1 8 1 1.2 0 1 9 36 0.3 0 5 31 10 112 0.1 1 4 108 11 2166 0.0 1 89 2077 12 924 0.0 1 29 895 13 37 0.0 1 0 37 14 2 0.0 1 0 2 15 4 0.0 1 0 4 20 1 0.0 1 0 1 127 2 0.0 1 2 149 5 0.0 1 4 1 150 1 0.0 1 1 152 19 0.0 1 7 12 154 1 0.0 1 0 1 159 1 0.0 1 1 171 1 0.0 1 1 191 6 0.0 1 0 6 208 2 0.0 1 2 210 45 0.0 1 39 6 211 1 0.0 1 1 222 1 0.0 1 0 1 230 1 0.0 1 1 257 1 0.0 1 1 260 1 0.0 1 1