This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.45 s (26 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 200,420 (7.9%) Reads that were too short: 3,733 (0.1%) Reads written (passing filters): 196,687 (7.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 55,567,563 bp (7.3%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 200420 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1217 621.6 0 1217 7 93001 621.6 0 93001 8 1565 621.6 0 1565 9 77504 621.6 0 77504 10 64 621.6 0 64 11 75 621.6 0 75 12 59 621.6 0 59 13 63 621.6 0 63 14 59 621.6 0 59 15 57 621.6 0 57 16 176 621.6 0 176 17 110 621.6 0 110 18 142 621.6 0 142 19 60 621.6 0 60 20 67 621.6 0 67 21 47 621.6 0 47 22 61 621.6 0 61 23 82 621.6 0 82 24 61 621.6 0 61 25 65 621.6 0 65 26 60 621.6 0 60 27 49 621.6 0 49 28 54 621.6 0 54 29 47 621.6 0 47 30 46 621.6 0 46 31 48 621.6 0 48 32 48 621.6 0 48 33 71 621.6 0 71 34 62 621.6 0 62 35 56 621.6 0 56 36 63 621.6 0 63 37 58 621.6 0 58 38 84 621.6 0 84 39 71 621.6 0 71 40 48 621.6 0 48 41 62 621.6 0 62 42 61 621.6 0 61 43 65 621.6 0 65 44 61 621.6 0 61 45 58 621.6 0 58 46 167 621.6 0 167 47 170 621.6 0 170 48 250 621.6 0 250 49 73 621.6 0 73 50 64 621.6 0 64 51 73 621.6 0 73 52 57 621.6 0 57 53 44 621.6 0 44 54 40 621.6 0 40 55 49 621.6 0 49 56 51 621.6 0 51 57 60 621.6 0 60 58 78 621.6 0 78 59 79 621.6 0 79 60 69 621.6 0 69 61 48 621.6 0 48 62 67 621.6 0 67 63 72 621.6 0 72 64 41 621.6 0 41 65 59 621.6 0 59 66 69 621.6 0 69 67 86 621.6 0 86 68 54 621.6 0 54 69 48 621.6 0 48 70 79 621.6 0 79 71 55 621.6 0 55 72 50 621.6 0 50 73 56 621.6 0 56 74 61 621.6 0 61 75 47 621.6 0 47 76 58 621.6 0 58 77 56 621.6 0 56 78 58 621.6 0 58 79 113 621.6 0 113 80 96 621.6 0 96 81 176 621.6 0 176 82 82 621.6 0 82 83 181 621.6 0 181 84 132 621.6 0 132 85 86 621.6 0 86 86 84 621.6 0 84 87 108 621.6 0 108 88 92 621.6 0 92 89 82 621.6 0 82 90 96 621.6 0 96 91 100 621.6 0 100 92 175 621.6 0 175 93 173 621.6 0 173 94 3960 621.6 0 3960 95 1408 621.6 0 1408 96 3125 621.6 0 3125 97 766 621.6 0 766 98 945 621.6 0 945 99 55 621.6 0 55 100 51 621.6 0 51 101 63 621.6 0 63 102 53 621.6 0 53 103 51 621.6 0 51 104 66 621.6 0 66 105 60 621.6 0 60 106 62 621.6 0 62 107 71 621.6 0 71 108 43 621.6 0 43 109 71 621.6 0 71 110 54 621.6 0 54 111 58 621.6 0 58 112 60 621.6 0 60 113 67 621.6 0 67 114 65 621.6 0 65 115 102 621.6 0 102 116 58 621.6 0 58 117 53 621.6 0 53 118 85 621.6 0 85 119 54 621.6 0 54 120 57 621.6 0 57 121 54 621.6 0 54 122 53 621.6 0 53 123 46 621.6 0 46 124 76 621.6 0 76 125 79 621.6 0 79 126 78 621.6 0 78 127 53 621.6 0 53 128 58 621.6 0 58 129 81 621.6 0 81 130 84 621.6 0 84 131 526 621.6 0 526 132 192 621.6 0 192 133 301 621.6 0 301 134 54 621.6 0 54 135 61 621.6 0 61 136 55 621.6 0 55 137 125 621.6 0 125 138 122 621.6 0 122 139 180 621.6 0 180 140 71 621.6 0 71 141 88 621.6 0 88 142 77 621.6 0 77 143 93 621.6 0 93 144 150 621.6 0 150 145 67 621.6 0 67 146 101 621.6 0 101 147 135 621.6 0 135 148 72 621.6 0 72 149 69 621.6 0 69 150 38 621.6 0 38 151 37 621.6 0 37 152 21 621.6 0 21 153 29 621.6 0 29 154 33 621.6 0 33 155 32 621.6 0 32 156 40 621.6 0 40 157 42 621.6 0 42 158 138 621.6 0 138 159 89 621.6 0 89 160 252 621.6 0 252 161 34 621.6 0 34 162 34 621.6 0 34 163 33 621.6 0 33 164 38 621.6 0 38 165 19 621.6 0 19 166 36 621.6 0 36 167 34 621.6 0 34 168 25 621.6 0 25 169 18 621.6 0 18 170 32 621.6 0 32 171 24 621.6 0 24 172 32 621.6 0 32 173 31 621.6 0 31 174 34 621.6 0 34 175 25 621.6 0 25 176 21 621.6 0 21 177 25 621.6 0 25 178 34 621.6 0 34 179 35 621.6 0 35 180 64 621.6 0 64 181 29 621.6 0 29 182 32 621.6 0 32 183 23 621.6 0 23 184 21 621.6 0 21 185 40 621.6 0 40 186 37 621.6 0 37 187 111 621.6 0 111 188 27 621.6 0 27 189 29 621.6 0 29 190 26 621.6 0 26 191 26 621.6 0 26 192 38 621.6 0 38 193 40 621.6 0 40 194 35 621.6 0 35 195 27 621.6 0 27 196 22 621.6 0 22 197 25 621.6 0 25 198 17 621.6 0 17 199 30 621.6 0 30 200 17 621.6 0 17 201 21 621.6 0 21 202 43 621.6 0 43 203 32 621.6 0 32 204 37 621.6 0 37 205 130 621.6 0 130 206 86 621.6 0 86 207 72 621.6 0 72 208 26 621.6 0 26 209 30 621.6 0 30 210 38 621.6 0 38 211 27 621.6 0 27 212 21 621.6 0 21 213 29 621.6 0 29 214 21 621.6 0 21 215 15 621.6 0 15 216 25 621.6 0 25 217 25 621.6 0 25 218 32 621.6 0 32 219 33 621.6 0 33 220 22 621.6 0 22 221 32 621.6 0 32 222 22 621.6 0 22 223 29 621.6 0 29 224 27 621.6 0 27 225 21 621.6 0 21 226 34 621.6 0 34 227 29 621.6 0 29 228 36 621.6 0 36 229 32 621.6 0 32 230 18 621.6 0 18 231 18 621.6 0 18 232 40 621.6 0 40 233 32 621.6 0 32 234 34 621.6 0 34 235 21 621.6 0 21 236 29 621.6 0 29 237 20 621.6 0 20 238 34 621.6 0 34 239 28 621.6 0 28 240 31 621.6 0 31 241 37 621.6 0 37 242 31 621.6 0 31 243 46 621.6 0 46 244 26 621.6 0 26 245 23 621.6 0 23 246 32 621.6 0 32 247 24 621.6 0 24 248 23 621.6 0 23 249 23 621.6 0 23 250 26 621.6 0 26 251 27 621.6 0 27 252 31 621.6 0 31 253 29 621.6 0 29 254 37 621.6 0 37 255 32 621.6 0 32 256 33 621.6 0 33 257 40 621.6 0 40 258 34 621.6 0 34 259 40 621.6 0 40 260 33 621.6 0 33 261 51 621.6 0 51 262 46 621.6 0 46 263 37 621.6 0 37 264 33 621.6 0 33 265 31 621.6 0 31 266 36 621.6 0 36 267 23 621.6 0 23 268 23 621.6 0 23 269 30 621.6 0 30 270 128 621.6 0 128 271 129 621.6 0 129 272 61 621.6 0 61 273 26 621.6 0 26 274 26 621.6 0 26 275 27 621.6 0 27 276 36 621.6 0 36 277 38 621.6 0 38 278 29 621.6 0 29 279 37 621.6 0 37 280 29 621.6 0 29 281 53 621.6 0 53 282 47 621.6 0 47 283 85 621.6 0 85 284 46 621.6 0 46 285 29 621.6 0 29 286 29 621.6 0 29 287 42 621.6 0 42 288 43 621.6 0 43 289 33 621.6 0 33 290 60 621.6 0 60 291 35 621.6 0 35 292 60 621.6 0 60 293 27 621.6 0 27 294 43 621.6 0 43 295 39 621.6 0 39 296 31 621.6 0 31 297 41 621.6 0 41 298 49 621.6 0 49 299 43 621.6 0 43 300 59 621.6 0 59 301 25 621.6 0 25 Finished in 8.95 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 196,687 Reads with adapters: 79,725 (40.5%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 79,710 (40.5%) Total basepairs processed: 55,567,563 bp Total written (filtered): 21,920,194 bp (39.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 79725 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 17 0.0 3 0 0 16 1 16 445 0.0 3 0 439 5 1 17 79033 0.0 3 77670 1309 42 12 18 108 0.0 3 6 94 6 2 19 31 0.0 3 0 0 28 3 20 49 0.0 3 0 0 0 49 30 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 1 60 3 0.0 3 0 0 0 3 63 2 0.0 3 0 0 1 1 65 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 1 121 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.85 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 79,710 Reads with adapters: 3,577 (4.5%) Reads written (passing filters): 79,710 (100.0%) Total basepairs processed: 21,920,194 bp Total written (filtered): 21,909,418 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3577 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.6% C: 6.7% G: 16.1% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3532 1245.5 0 3532 4 45 311.4 0 45