This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.20 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 211,480 (8.3%) Reads that were too short: 31,876 (1.3%) Reads written (passing filters): 179,604 (7.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 32,428,283 bp (4.2%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 211480 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 345 621.6 0 345 7 8036 621.6 0 8036 8 355 621.6 0 355 9 6794 621.6 0 6794 10 57 621.6 0 57 11 54 621.6 0 54 12 32 621.6 0 32 13 47 621.6 0 47 14 131 621.6 0 131 15 53 621.6 0 53 16 94 621.6 0 94 17 39 621.6 0 39 18 40 621.6 0 40 19 37 621.6 0 37 20 30 621.6 0 30 21 74 621.6 0 74 22 56 621.6 0 56 23 64 621.6 0 64 24 82 621.6 0 82 25 77 621.6 0 77 26 40 621.6 0 40 27 57 621.6 0 57 28 51 621.6 0 51 29 42 621.6 0 42 30 47 621.6 0 47 31 50 621.6 0 50 32 51 621.6 0 51 33 51 621.6 0 51 34 64 621.6 0 64 35 45 621.6 0 45 36 65 621.6 0 65 37 55 621.6 0 55 38 65 621.6 0 65 39 68 621.6 0 68 40 61 621.6 0 61 41 69 621.6 0 69 42 74 621.6 0 74 43 57 621.6 0 57 44 51 621.6 0 51 45 61 621.6 0 61 46 87 621.6 0 87 47 54 621.6 0 54 48 70 621.6 0 70 49 52 621.6 0 52 50 55 621.6 0 55 51 75 621.6 0 75 52 68 621.6 0 68 53 68 621.6 0 68 54 62 621.6 0 62 55 66 621.6 0 66 56 158 621.6 0 158 57 171 621.6 0 171 58 319 621.6 0 319 59 543 621.6 0 543 60 174 621.6 0 174 61 330 621.6 0 330 62 56 621.6 0 56 63 60 621.6 0 60 64 74 621.6 0 74 65 77 621.6 0 77 66 56 621.6 0 56 67 101 621.6 0 101 68 51 621.6 0 51 69 83 621.6 0 83 70 71 621.6 0 71 71 56 621.6 0 56 72 87 621.6 0 87 73 118 621.6 0 118 74 84 621.6 0 84 75 66 621.6 0 66 76 70 621.6 0 70 77 192 621.6 0 192 78 47 621.6 0 47 79 63 621.6 0 63 80 57 621.6 0 57 81 55 621.6 0 55 82 67 621.6 0 67 83 105 621.6 0 105 84 116 621.6 0 116 85 161 621.6 0 161 86 109 621.6 0 109 87 169 621.6 0 169 88 86 621.6 0 86 89 119 621.6 0 119 90 124 621.6 0 124 91 147 621.6 0 147 92 91 621.6 0 91 93 69 621.6 0 69 94 102 621.6 0 102 95 89 621.6 0 89 96 107 621.6 0 107 97 279 621.6 0 279 98 89 621.6 0 89 99 128 621.6 0 128 100 127 621.6 0 127 101 120 621.6 0 120 102 156 621.6 0 156 103 121 621.6 0 121 104 102 621.6 0 102 105 114 621.6 0 114 106 486 621.6 0 486 107 351 621.6 0 351 108 673 621.6 0 673 109 321 621.6 0 321 110 518 621.6 0 518 111 543 621.6 0 543 112 1220 621.6 0 1220 113 2158 621.6 0 2158 114 27461 621.6 0 27461 115 15206 621.6 0 15206 116 25459 621.6 0 25459 117 211 621.6 0 211 118 139 621.6 0 139 119 65 621.6 0 65 120 83 621.6 0 83 121 54 621.6 0 54 122 37 621.6 0 37 123 80 621.6 0 80 124 63 621.6 0 63 125 80 621.6 0 80 126 195 621.6 0 195 127 273 621.6 0 273 128 160 621.6 0 160 129 201 621.6 0 201 130 159 621.6 0 159 131 141 621.6 0 141 132 85 621.6 0 85 133 76 621.6 0 76 134 79 621.6 0 79 135 147 621.6 0 147 136 290 621.6 0 290 137 144 621.6 0 144 138 131 621.6 0 131 139 105 621.6 0 105 140 423 621.6 0 423 141 899 621.6 0 899 142 634 621.6 0 634 143 686 621.6 0 686 144 307 621.6 0 307 145 430 621.6 0 430 146 849 621.6 0 849 147 6670 621.6 0 6670 148 3762 621.6 0 3762 149 10180 621.6 0 10180 150 427 621.6 0 427 151 721 621.6 0 721 152 1634 621.6 0 1634 153 10945 621.6 0 10945 154 10041 621.6 0 10041 155 20026 621.6 0 20026 156 401 621.6 0 401 157 244 621.6 0 244 158 235 621.6 0 235 159 127 621.6 0 127 160 134 621.6 0 134 161 150 621.6 0 150 162 193 621.6 0 193 163 166 621.6 0 166 164 140 621.6 0 140 165 97 621.6 0 97 166 82 621.6 0 82 167 197 621.6 0 197 168 548 621.6 0 548 169 394 621.6 0 394 170 641 621.6 0 641 171 166 621.6 0 166 172 75 621.6 0 75 173 48 621.6 0 48 174 65 621.6 0 65 175 74 621.6 0 74 176 48 621.6 0 48 177 60 621.6 0 60 178 87 621.6 0 87 179 65 621.6 0 65 180 72 621.6 0 72 181 117 621.6 0 117 182 121 621.6 0 121 183 845 621.6 0 845 184 619 621.6 0 619 185 875 621.6 0 875 186 680 621.6 0 680 187 292 621.6 0 292 188 126 621.6 0 126 189 66 621.6 0 66 190 111 621.6 0 111 191 143 621.6 0 143 192 82 621.6 0 82 193 74 621.6 0 74 194 57 621.6 0 57 195 41 621.6 0 41 196 50 621.6 0 50 197 72 621.6 0 72 198 77 621.6 0 77 199 83 621.6 0 83 200 69 621.6 0 69 201 73 621.6 0 73 202 93 621.6 0 93 203 117 621.6 0 117 204 97 621.6 0 97 205 58 621.6 0 58 206 54 621.6 0 54 207 59 621.6 0 59 208 45 621.6 0 45 209 54 621.6 0 54 210 34 621.6 0 34 211 64 621.6 0 64 212 62 621.6 0 62 213 84 621.6 0 84 214 70 621.6 0 70 215 186 621.6 0 186 216 436 621.6 0 436 217 331 621.6 0 331 218 558 621.6 0 558 219 52 621.6 0 52 220 44 621.6 0 44 221 42 621.6 0 42 222 54 621.6 0 54 223 49 621.6 0 49 224 62 621.6 0 62 225 66 621.6 0 66 226 120 621.6 0 120 227 109 621.6 0 109 228 105 621.6 0 105 229 63 621.6 0 63 230 74 621.6 0 74 231 75 621.6 0 75 232 101 621.6 0 101 233 247 621.6 0 247 234 242 621.6 0 242 235 287 621.6 0 287 236 152 621.6 0 152 237 140 621.6 0 140 238 73 621.6 0 73 239 72 621.6 0 72 240 48 621.6 0 48 241 68 621.6 0 68 242 88 621.6 0 88 243 71 621.6 0 71 244 137 621.6 0 137 245 128 621.6 0 128 246 159 621.6 0 159 247 93 621.6 0 93 248 102 621.6 0 102 249 191 621.6 0 191 250 163 621.6 0 163 251 208 621.6 0 208 252 72 621.6 0 72 253 125 621.6 0 125 254 86 621.6 0 86 255 96 621.6 0 96 256 73 621.6 0 73 257 70 621.6 0 70 258 78 621.6 0 78 259 87 621.6 0 87 260 84 621.6 0 84 261 125 621.6 0 125 262 116 621.6 0 116 263 97 621.6 0 97 264 91 621.6 0 91 265 98 621.6 0 98 266 92 621.6 0 92 267 142 621.6 0 142 268 164 621.6 0 164 269 158 621.6 0 158 270 252 621.6 0 252 271 411 621.6 0 411 272 240 621.6 0 240 273 154 621.6 0 154 274 156 621.6 0 156 275 160 621.6 0 160 276 238 621.6 0 238 277 199 621.6 0 199 278 360 621.6 0 360 279 889 621.6 0 889 280 1646 621.6 0 1646 281 6435 621.6 0 6435 282 3354 621.6 0 3354 283 4233 621.6 0 4233 284 173 621.6 0 173 285 182 621.6 0 182 286 148 621.6 0 148 287 116 621.6 0 116 288 95 621.6 0 95 289 137 621.6 0 137 290 454 621.6 0 454 291 423 621.6 0 423 292 734 621.6 0 734 293 402 621.6 0 402 294 264 621.6 0 264 295 422 621.6 0 422 296 391 621.6 0 391 297 428 621.6 0 428 298 485 621.6 0 485 299 147 621.6 0 147 300 75 621.6 0 75 301 132 621.6 0 132 Finished in 6.83 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 179,604 Reads with adapters: 7,294 (4.1%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 7,284 (4.1%) Total basepairs processed: 32,428,283 bp Total written (filtered): 1,976,983 bp (6.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7294 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 5 1 1 19 95 0.0 3 0 80 6 7 2 20 7090 0.0 4 6608 373 76 26 7 21 24 0.0 4 8 7 4 4 1 22 2 0.0 4 0 0 2 23 19 0.0 4 0 0 0 16 3 27 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 34 0.0 4 0 0 0 0 34 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 7,284 Reads with adapters: 1,895 (26.0%) Reads written (passing filters): 7,284 (100.0%) Total basepairs processed: 1,976,983 bp Total written (filtered): 1,955,519 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1895 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 8.4% G: 87.9% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 113.8 0 4 4 1 28.5 0 1 5 1 7.1 0 1 9 493 0.0 0 34 459 10 1048 0.0 1 55 993 11 324 0.0 1 5 319 12 9 0.0 1 0 9 13 2 0.0 1 0 2 208 9 0.0 1 5 4 209 2 0.0 1 1 1 269 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.