This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.64 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 232,463 (9.1%) Reads that were too short: 14,518 (0.6%) Reads written (passing filters): 217,945 (8.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 39,472,594 bp (5.2%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 232463 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 260 621.6 0 260 7 7720 621.6 0 7720 8 333 621.6 0 333 9 8055 621.6 0 8055 10 41 621.6 0 41 11 39 621.6 0 39 12 40 621.6 0 40 13 31 621.6 0 31 14 46 621.6 0 46 15 50 621.6 0 50 16 33 621.6 0 33 17 47 621.6 0 47 18 35 621.6 0 35 19 40 621.6 0 40 20 35 621.6 0 35 21 63 621.6 0 63 22 46 621.6 0 46 23 49 621.6 0 49 24 55 621.6 0 55 25 70 621.6 0 70 26 49 621.6 0 49 27 46 621.6 0 46 28 48 621.6 0 48 29 33 621.6 0 33 30 53 621.6 0 53 31 62 621.6 0 62 32 71 621.6 0 71 33 67 621.6 0 67 34 63 621.6 0 63 35 45 621.6 0 45 36 56 621.6 0 56 37 40 621.6 0 40 38 51 621.6 0 51 39 55 621.6 0 55 40 60 621.6 0 60 41 49 621.6 0 49 42 48 621.6 0 48 43 45 621.6 0 45 44 47 621.6 0 47 45 35 621.6 0 35 46 67 621.6 0 67 47 41 621.6 0 41 48 53 621.6 0 53 49 53 621.6 0 53 50 55 621.6 0 55 51 111 621.6 0 111 52 67 621.6 0 67 53 61 621.6 0 61 54 52 621.6 0 52 55 46 621.6 0 46 56 78 621.6 0 78 57 92 621.6 0 92 58 105 621.6 0 105 59 180 621.6 0 180 60 79 621.6 0 79 61 1061 621.6 0 1061 62 78 621.6 0 78 63 52 621.6 0 52 64 62 621.6 0 62 65 65 621.6 0 65 66 53 621.6 0 53 67 50 621.6 0 50 68 53 621.6 0 53 69 40 621.6 0 40 70 68 621.6 0 68 71 59 621.6 0 59 72 48 621.6 0 48 73 63 621.6 0 63 74 55 621.6 0 55 75 46 621.6 0 46 76 57 621.6 0 57 77 250 621.6 0 250 78 55 621.6 0 55 79 62 621.6 0 62 80 52 621.6 0 52 81 68 621.6 0 68 82 46 621.6 0 46 83 57 621.6 0 57 84 92 621.6 0 92 85 97 621.6 0 97 86 122 621.6 0 122 87 207 621.6 0 207 88 80 621.6 0 80 89 55 621.6 0 55 90 57 621.6 0 57 91 66 621.6 0 66 92 73 621.6 0 73 93 56 621.6 0 56 94 102 621.6 0 102 95 69 621.6 0 69 96 139 621.6 0 139 97 314 621.6 0 314 98 99 621.6 0 99 99 164 621.6 0 164 100 115 621.6 0 115 101 134 621.6 0 134 102 215 621.6 0 215 103 126 621.6 0 126 104 114 621.6 0 114 105 83 621.6 0 83 106 221 621.6 0 221 107 264 621.6 0 264 108 417 621.6 0 417 109 401 621.6 0 401 110 618 621.6 0 618 111 772 621.6 0 772 112 1627 621.6 0 1627 113 2928 621.6 0 2928 114 40687 621.6 0 40687 115 22041 621.6 0 22041 116 36718 621.6 0 36718 117 207 621.6 0 207 118 92 621.6 0 92 119 79 621.6 0 79 120 98 621.6 0 98 121 66 621.6 0 66 122 45 621.6 0 45 123 70 621.6 0 70 124 48 621.6 0 48 125 76 621.6 0 76 126 170 621.6 0 170 127 324 621.6 0 324 128 101 621.6 0 101 129 169 621.6 0 169 130 152 621.6 0 152 131 87 621.6 0 87 132 62 621.6 0 62 133 69 621.6 0 69 134 83 621.6 0 83 135 171 621.6 0 171 136 263 621.6 0 263 137 88 621.6 0 88 138 87 621.6 0 87 139 90 621.6 0 90 140 204 621.6 0 204 141 626 621.6 0 626 142 299 621.6 0 299 143 704 621.6 0 704 144 279 621.6 0 279 145 405 621.6 0 405 146 843 621.6 0 843 147 8629 621.6 0 8629 148 4503 621.6 0 4503 149 12509 621.6 0 12509 150 320 621.6 0 320 151 686 621.6 0 686 152 1574 621.6 0 1574 153 13336 621.6 0 13336 154 11365 621.6 0 11365 155 23709 621.6 0 23709 156 236 621.6 0 236 157 139 621.6 0 139 158 90 621.6 0 90 159 38 621.6 0 38 160 67 621.6 0 67 161 78 621.6 0 78 162 115 621.6 0 115 163 92 621.6 0 92 164 44 621.6 0 44 165 74 621.6 0 74 166 59 621.6 0 59 167 76 621.6 0 76 168 122 621.6 0 122 169 107 621.6 0 107 170 226 621.6 0 226 171 140 621.6 0 140 172 64 621.6 0 64 173 49 621.6 0 49 174 37 621.6 0 37 175 39 621.6 0 39 176 36 621.6 0 36 177 59 621.6 0 59 178 43 621.6 0 43 179 39 621.6 0 39 180 58 621.6 0 58 181 69 621.6 0 69 182 71 621.6 0 71 183 312 621.6 0 312 184 230 621.6 0 230 185 307 621.6 0 307 186 228 621.6 0 228 187 81 621.6 0 81 188 64 621.6 0 64 189 45 621.6 0 45 190 51 621.6 0 51 191 154 621.6 0 154 192 61 621.6 0 61 193 65 621.6 0 65 194 43 621.6 0 43 195 50 621.6 0 50 196 30 621.6 0 30 197 60 621.6 0 60 198 61 621.6 0 61 199 42 621.6 0 42 200 39 621.6 0 39 201 43 621.6 0 43 202 56 621.6 0 56 203 61 621.6 0 61 204 51 621.6 0 51 205 57 621.6 0 57 206 41 621.6 0 41 207 44 621.6 0 44 208 47 621.6 0 47 209 40 621.6 0 40 210 30 621.6 0 30 211 32 621.6 0 32 212 53 621.6 0 53 213 44 621.6 0 44 214 41 621.6 0 41 215 86 621.6 0 86 216 137 621.6 0 137 217 119 621.6 0 119 218 215 621.6 0 215 219 43 621.6 0 43 220 46 621.6 0 46 221 40 621.6 0 40 222 61 621.6 0 61 223 40 621.6 0 40 224 38 621.6 0 38 225 61 621.6 0 61 226 91 621.6 0 91 227 68 621.6 0 68 228 77 621.6 0 77 229 48 621.6 0 48 230 62 621.6 0 62 231 55 621.6 0 55 232 49 621.6 0 49 233 82 621.6 0 82 234 101 621.6 0 101 235 110 621.6 0 110 236 88 621.6 0 88 237 55 621.6 0 55 238 65 621.6 0 65 239 46 621.6 0 46 240 48 621.6 0 48 241 49 621.6 0 49 242 33 621.6 0 33 243 41 621.6 0 41 244 47 621.6 0 47 245 56 621.6 0 56 246 60 621.6 0 60 247 44 621.6 0 44 248 53 621.6 0 53 249 70 621.6 0 70 250 50 621.6 0 50 251 62 621.6 0 62 252 38 621.6 0 38 253 70 621.6 0 70 254 57 621.6 0 57 255 61 621.6 0 61 256 44 621.6 0 44 257 50 621.6 0 50 258 53 621.6 0 53 259 49 621.6 0 49 260 44 621.6 0 44 261 60 621.6 0 60 262 55 621.6 0 55 263 58 621.6 0 58 264 60 621.6 0 60 265 60 621.6 0 60 266 66 621.6 0 66 267 94 621.6 0 94 268 90 621.6 0 90 269 68 621.6 0 68 270 104 621.6 0 104 271 110 621.6 0 110 272 91 621.6 0 91 273 84 621.6 0 84 274 84 621.6 0 84 275 65 621.6 0 65 276 97 621.6 0 97 277 112 621.6 0 112 278 156 621.6 0 156 279 455 621.6 0 455 280 732 621.6 0 732 281 2770 621.6 0 2770 282 1572 621.6 0 1572 283 1881 621.6 0 1881 284 90 621.6 0 90 285 83 621.6 0 83 286 83 621.6 0 83 287 66 621.6 0 66 288 67 621.6 0 67 289 68 621.6 0 68 290 207 621.6 0 207 291 221 621.6 0 221 292 313 621.6 0 313 293 149 621.6 0 149 294 67 621.6 0 67 295 109 621.6 0 109 296 108 621.6 0 108 297 87 621.6 0 87 298 100 621.6 0 100 299 73 621.6 0 73 300 61 621.6 0 61 301 83 621.6 0 83 Finished in 7.56 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 217,945 Reads with adapters: 7,741 (3.6%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 7,700 (3.5%) Total basepairs processed: 39,472,594 bp Total written (filtered): 2,127,974 bp (5.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7741 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 51 0.0 3 0 50 0 1 17 7578 0.0 3 7404 166 8 18 21 0.0 3 4 17 19 2 0.0 3 0 0 2 20 14 0.0 3 1 0 0 13 23 1 0.0 3 1 33 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 77 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 85 10 0.0 3 0 0 0 10 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 6 0.0 3 0 0 0 6 175 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 210 1 0.0 3 0 1 217 12 0.0 3 0 0 0 12 222 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 7,700 Reads with adapters: 16 (0.2%) Reads written (passing filters): 7,700 (100.0%) Total basepairs processed: 2,127,974 bp Total written (filtered): 2,127,923 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 16 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.2% C: 18.8% G: 68.8% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 120.3 0 14 4 1 30.1 0 1 5 1 7.5 0 1