This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.66 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 225,548 (8.9%) Reads that were too short: 35,654 (1.4%) Reads written (passing filters): 189,894 (7.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 53,340,234 bp (7.0%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 225548 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4015 621.6 0 4015 7 73515 621.6 0 73515 8 2472 621.6 0 2472 9 88700 621.6 0 88700 10 121 621.6 0 121 11 61 621.6 0 61 12 200 621.6 0 200 13 34 621.6 0 34 14 45 621.6 0 45 15 58 621.6 0 58 16 56 621.6 0 56 17 138 621.6 0 138 18 69 621.6 0 69 19 108 621.6 0 108 20 56 621.6 0 56 21 45 621.6 0 45 22 52 621.6 0 52 23 45 621.6 0 45 24 54 621.6 0 54 25 41 621.6 0 41 26 45 621.6 0 45 27 41 621.6 0 41 28 57 621.6 0 57 29 69 621.6 0 69 30 110 621.6 0 110 31 63 621.6 0 63 32 73 621.6 0 73 33 81 621.6 0 81 34 50 621.6 0 50 35 44 621.6 0 44 36 68 621.6 0 68 37 70 621.6 0 70 38 65 621.6 0 65 39 69 621.6 0 69 40 79 621.6 0 79 41 55 621.6 0 55 42 68 621.6 0 68 43 55 621.6 0 55 44 41 621.6 0 41 45 54 621.6 0 54 46 53 621.6 0 53 47 85 621.6 0 85 48 88 621.6 0 88 49 100 621.6 0 100 50 48 621.6 0 48 51 57 621.6 0 57 52 52 621.6 0 52 53 64 621.6 0 64 54 76 621.6 0 76 55 62 621.6 0 62 56 50 621.6 0 50 57 120 621.6 0 120 58 51 621.6 0 51 59 45 621.6 0 45 60 80 621.6 0 80 61 60 621.6 0 60 62 77 621.6 0 77 63 68 621.6 0 68 64 87 621.6 0 87 65 28 621.6 0 28 66 42 621.6 0 42 67 49 621.6 0 49 68 67 621.6 0 67 69 60 621.6 0 60 70 80 621.6 0 80 71 96 621.6 0 96 72 63 621.6 0 63 73 107 621.6 0 107 74 52 621.6 0 52 75 61 621.6 0 61 76 43 621.6 0 43 77 59 621.6 0 59 78 49 621.6 0 49 79 81 621.6 0 81 80 68 621.6 0 68 81 77 621.6 0 77 82 86 621.6 0 86 83 50 621.6 0 50 84 64 621.6 0 64 85 57 621.6 0 57 86 57 621.6 0 57 87 57 621.6 0 57 88 42 621.6 0 42 89 75 621.6 0 75 90 57 621.6 0 57 91 48 621.6 0 48 92 77 621.6 0 77 93 79 621.6 0 79 94 75 621.6 0 75 95 62 621.6 0 62 96 67 621.6 0 67 97 54 621.6 0 54 98 45 621.6 0 45 99 62 621.6 0 62 100 46 621.6 0 46 101 56 621.6 0 56 102 43 621.6 0 43 103 50 621.6 0 50 104 34 621.6 0 34 105 48 621.6 0 48 106 54 621.6 0 54 107 64 621.6 0 64 108 31 621.6 0 31 109 59 621.6 0 59 110 49 621.6 0 49 111 59 621.6 0 59 112 39 621.6 0 39 113 57 621.6 0 57 114 48 621.6 0 48 115 71 621.6 0 71 116 92 621.6 0 92 117 122 621.6 0 122 118 59 621.6 0 59 119 61 621.6 0 61 120 84 621.6 0 84 121 71 621.6 0 71 122 64 621.6 0 64 123 131 621.6 0 131 124 81 621.6 0 81 125 98 621.6 0 98 126 74 621.6 0 74 127 92 621.6 0 92 128 164 621.6 0 164 129 242 621.6 0 242 130 3700 621.6 0 3700 131 1554 621.6 0 1554 132 1911 621.6 0 1911 133 204 621.6 0 204 134 103 621.6 0 103 135 108 621.6 0 108 136 368 621.6 0 368 137 376 621.6 0 376 138 523 621.6 0 523 139 132 621.6 0 132 140 54 621.6 0 54 141 32 621.6 0 32 142 65 621.6 0 65 143 54 621.6 0 54 144 49 621.6 0 49 145 40 621.6 0 40 146 66 621.6 0 66 147 47 621.6 0 47 148 33 621.6 0 33 149 62 621.6 0 62 150 57 621.6 0 57 151 108 621.6 0 108 152 83 621.6 0 83 153 115 621.6 0 115 154 61 621.6 0 61 155 54 621.6 0 54 156 44 621.6 0 44 157 44 621.6 0 44 158 43 621.6 0 43 159 46 621.6 0 46 160 34 621.6 0 34 161 42 621.6 0 42 162 42 621.6 0 42 163 48 621.6 0 48 164 53 621.6 0 53 165 68 621.6 0 68 166 58 621.6 0 58 167 53 621.6 0 53 168 57 621.6 0 57 169 43 621.6 0 43 170 35 621.6 0 35 171 41 621.6 0 41 172 33 621.6 0 33 173 40 621.6 0 40 174 42 621.6 0 42 175 39 621.6 0 39 176 56 621.6 0 56 177 69 621.6 0 69 178 68 621.6 0 68 179 69 621.6 0 69 180 33 621.6 0 33 181 51 621.6 0 51 182 53 621.6 0 53 183 50 621.6 0 50 184 47 621.6 0 47 185 62 621.6 0 62 186 305 621.6 0 305 187 233 621.6 0 233 188 137 621.6 0 137 189 107 621.6 0 107 190 82 621.6 0 82 191 68 621.6 0 68 192 54 621.6 0 54 193 44 621.6 0 44 194 78 621.6 0 78 195 60 621.6 0 60 196 59 621.6 0 59 197 98 621.6 0 98 198 71 621.6 0 71 199 52 621.6 0 52 200 57 621.6 0 57 201 63 621.6 0 63 202 56 621.6 0 56 203 58 621.6 0 58 204 69 621.6 0 69 205 87 621.6 0 87 206 78 621.6 0 78 207 62 621.6 0 62 208 39 621.6 0 39 209 59 621.6 0 59 210 58 621.6 0 58 211 90 621.6 0 90 212 367 621.6 0 367 213 406 621.6 0 406 214 293 621.6 0 293 215 79 621.6 0 79 216 175 621.6 0 175 217 166 621.6 0 166 218 301 621.6 0 301 219 214 621.6 0 214 220 149 621.6 0 149 221 51 621.6 0 51 222 49 621.6 0 49 223 48 621.6 0 48 224 59 621.6 0 59 225 64 621.6 0 64 226 68 621.6 0 68 227 50 621.6 0 50 228 74 621.6 0 74 229 69 621.6 0 69 230 66 621.6 0 66 231 60 621.6 0 60 232 105 621.6 0 105 233 84 621.6 0 84 234 122 621.6 0 122 235 156 621.6 0 156 236 248 621.6 0 248 237 361 621.6 0 361 238 320 621.6 0 320 239 157 621.6 0 157 240 77 621.6 0 77 241 284 621.6 0 284 242 230 621.6 0 230 243 1106 621.6 0 1106 244 732 621.6 0 732 245 831 621.6 0 831 246 258 621.6 0 258 247 162 621.6 0 162 248 134 621.6 0 134 249 103 621.6 0 103 250 234 621.6 0 234 251 167 621.6 0 167 252 138 621.6 0 138 253 71 621.6 0 71 254 125 621.6 0 125 255 159 621.6 0 159 256 536 621.6 0 536 257 436 621.6 0 436 258 366 621.6 0 366 259 233 621.6 0 233 260 879 621.6 0 879 261 612 621.6 0 612 262 632 621.6 0 632 263 138 621.6 0 138 264 86 621.6 0 86 265 133 621.6 0 133 266 126 621.6 0 126 267 120 621.6 0 120 268 100 621.6 0 100 269 95 621.6 0 95 270 108 621.6 0 108 271 155 621.6 0 155 272 169 621.6 0 169 273 218 621.6 0 218 274 135 621.6 0 135 275 171 621.6 0 171 276 215 621.6 0 215 277 165 621.6 0 165 278 136 621.6 0 136 279 153 621.6 0 153 280 216 621.6 0 216 281 200 621.6 0 200 282 238 621.6 0 238 283 320 621.6 0 320 284 413 621.6 0 413 285 488 621.6 0 488 286 4974 621.6 0 4974 287 4357 621.6 0 4357 288 5766 621.6 0 5766 289 138 621.6 0 138 290 95 621.6 0 95 291 108 621.6 0 108 292 121 621.6 0 121 293 140 621.6 0 140 294 128 621.6 0 128 295 145 621.6 0 145 296 234 621.6 0 234 297 422 621.6 0 422 298 497 621.6 0 497 299 476 621.6 0 476 300 162 621.6 0 162 301 71 621.6 0 71 Finished in 9.68 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 189,894 Reads with adapters: 72,531 (38.2%) Reads that were too short: 86 (0.0%) Reads written (passing filters): 72,445 (38.2%) Total basepairs processed: 53,340,234 bp Total written (filtered): 19,783,345 bp (37.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 72531 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 43 0.0 3 0 0 28 8 7 19 825 0.0 3 0 685 83 42 15 20 70589 0.0 4 65376 4134 812 210 57 21 137 0.0 4 9 112 11 4 1 22 22 0.0 4 4 1 9 4 4 23 183 0.0 4 2 0 2 131 48 24 3 0.0 4 0 1 0 1 1 35 1 0.0 4 1 39 1 0.0 4 0 0 0 1 46 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 95 2 0.0 4 0 0 0 0 2 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 565 0.0 4 0 0 0 0 565 128 1 0.0 4 0 0 0 0 1 129 39 0.0 4 0 0 0 0 39 130 2 0.0 4 0 0 0 0 2 132 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 139 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 9 0.0 4 0 0 0 1 8 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 1 182 1 0.0 4 0 0 0 1 192 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 3 0.0 4 0 0 0 0 3 208 2 0.0 4 0 0 0 0 2 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 24 0.0 4 0 0 0 1 23 229 12 0.0 4 0 0 0 1 11 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 4 0.0 4 0 0 0 0 4 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 239 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 255 2 0.0 4 0 0 0 0 2 258 4 0.0 4 0 0 0 0 4 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.52 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 72,445 Reads with adapters: 4,263 (5.9%) Reads written (passing filters): 72,445 (100.0%) Total basepairs processed: 19,783,345 bp Total written (filtered): 19,728,072 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4263 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.3% C: 14.0% G: 74.6% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 404 1132.0 0 404 4 83 283.0 0 83 5 2 70.7 0 2 6 1 17.7 0 1 9 25 0.3 0 4 21 10 94 0.1 1 1 93 11 2768 0.0 1 92 2676 12 747 0.0 1 22 725 13 47 0.0 1 2 45 14 4 0.0 1 0 4 15 3 0.0 1 0 3 16 1 0.0 1 0 1 18 1 0.0 1 1 20 5 0.0 1 3 2 21 3 0.0 1 2 1 24 9 0.0 1 8 1 45 1 0.0 1 1 73 1 0.0 1 0 1 93 1 0.0 1 1 149 3 0.0 1 3 150 1 0.0 1 1 152 19 0.0 1 17 2 153 1 0.0 1 0 1 171 4 0.0 1 4 202 1 0.0 1 1 210 28 0.0 1 22 6 211 2 0.0 1 2 228 1 0.0 1 0 1 230 1 0.0 1 1 243 1 0.0 1 1 273 1 0.0 1 0 1