This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.93 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 232,214 (9.1%) Reads that were too short: 35,111 (1.4%) Reads written (passing filters): 197,103 (7.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 55,862,376 bp (7.3%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 232214 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4408 621.6 0 4408 7 95269 621.6 0 95269 8 2019 621.6 0 2019 9 76519 621.6 0 76519 10 113 621.6 0 113 11 67 621.6 0 67 12 137 621.6 0 137 13 51 621.6 0 51 14 75 621.6 0 75 15 58 621.6 0 58 16 66 621.6 0 66 17 75 621.6 0 75 18 70 621.6 0 70 19 82 621.6 0 82 20 86 621.6 0 86 21 60 621.6 0 60 22 69 621.6 0 69 23 66 621.6 0 66 24 62 621.6 0 62 25 68 621.6 0 68 26 64 621.6 0 64 27 60 621.6 0 60 28 84 621.6 0 84 29 49 621.6 0 49 30 65 621.6 0 65 31 51 621.6 0 51 32 60 621.6 0 60 33 104 621.6 0 104 34 63 621.6 0 63 35 52 621.6 0 52 36 70 621.6 0 70 37 75 621.6 0 75 38 71 621.6 0 71 39 77 621.6 0 77 40 86 621.6 0 86 41 105 621.6 0 105 42 91 621.6 0 91 43 84 621.6 0 84 44 48 621.6 0 48 45 52 621.6 0 52 46 68 621.6 0 68 47 90 621.6 0 90 48 67 621.6 0 67 49 74 621.6 0 74 50 61 621.6 0 61 51 49 621.6 0 49 52 86 621.6 0 86 53 83 621.6 0 83 54 87 621.6 0 87 55 78 621.6 0 78 56 50 621.6 0 50 57 119 621.6 0 119 58 95 621.6 0 95 59 65 621.6 0 65 60 74 621.6 0 74 61 77 621.6 0 77 62 77 621.6 0 77 63 88 621.6 0 88 64 87 621.6 0 87 65 56 621.6 0 56 66 63 621.6 0 63 67 73 621.6 0 73 68 76 621.6 0 76 69 71 621.6 0 71 70 49 621.6 0 49 71 124 621.6 0 124 72 63 621.6 0 63 73 106 621.6 0 106 74 40 621.6 0 40 75 52 621.6 0 52 76 39 621.6 0 39 77 72 621.6 0 72 78 50 621.6 0 50 79 50 621.6 0 50 80 70 621.6 0 70 81 62 621.6 0 62 82 67 621.6 0 67 83 46 621.6 0 46 84 60 621.6 0 60 85 67 621.6 0 67 86 53 621.6 0 53 87 51 621.6 0 51 88 52 621.6 0 52 89 51 621.6 0 51 90 43 621.6 0 43 91 56 621.6 0 56 92 63 621.6 0 63 93 58 621.6 0 58 94 54 621.6 0 54 95 77 621.6 0 77 96 53 621.6 0 53 97 61 621.6 0 61 98 46 621.6 0 46 99 60 621.6 0 60 100 66 621.6 0 66 101 43 621.6 0 43 102 45 621.6 0 45 103 55 621.6 0 55 104 50 621.6 0 50 105 54 621.6 0 54 106 52 621.6 0 52 107 73 621.6 0 73 108 41 621.6 0 41 109 59 621.6 0 59 110 45 621.6 0 45 111 56 621.6 0 56 112 44 621.6 0 44 113 59 621.6 0 59 114 54 621.6 0 54 115 39 621.6 0 39 116 49 621.6 0 49 117 126 621.6 0 126 118 54 621.6 0 54 119 34 621.6 0 34 120 83 621.6 0 83 121 48 621.6 0 48 122 34 621.6 0 34 123 120 621.6 0 120 124 66 621.6 0 66 125 76 621.6 0 76 126 49 621.6 0 49 127 68 621.6 0 68 128 121 621.6 0 121 129 128 621.6 0 128 130 3852 621.6 0 3852 131 1448 621.6 0 1448 132 1870 621.6 0 1870 133 160 621.6 0 160 134 61 621.6 0 61 135 71 621.6 0 71 136 108 621.6 0 108 137 189 621.6 0 189 138 234 621.6 0 234 139 124 621.6 0 124 140 43 621.6 0 43 141 26 621.6 0 26 142 43 621.6 0 43 143 34 621.6 0 34 144 26 621.6 0 26 145 28 621.6 0 28 146 43 621.6 0 43 147 47 621.6 0 47 148 58 621.6 0 58 149 41 621.6 0 41 150 47 621.6 0 47 151 52 621.6 0 52 152 63 621.6 0 63 153 52 621.6 0 52 154 43 621.6 0 43 155 50 621.6 0 50 156 26 621.6 0 26 157 28 621.6 0 28 158 37 621.6 0 37 159 45 621.6 0 45 160 50 621.6 0 50 161 26 621.6 0 26 162 26 621.6 0 26 163 40 621.6 0 40 164 35 621.6 0 35 165 34 621.6 0 34 166 49 621.6 0 49 167 36 621.6 0 36 168 47 621.6 0 47 169 28 621.6 0 28 170 30 621.6 0 30 171 34 621.6 0 34 172 31 621.6 0 31 173 39 621.6 0 39 174 30 621.6 0 30 175 28 621.6 0 28 176 36 621.6 0 36 177 47 621.6 0 47 178 62 621.6 0 62 179 56 621.6 0 56 180 26 621.6 0 26 181 45 621.6 0 45 182 44 621.6 0 44 183 58 621.6 0 58 184 39 621.6 0 39 185 48 621.6 0 48 186 118 621.6 0 118 187 72 621.6 0 72 188 56 621.6 0 56 189 59 621.6 0 59 190 44 621.6 0 44 191 36 621.6 0 36 192 36 621.6 0 36 193 47 621.6 0 47 194 46 621.6 0 46 195 41 621.6 0 41 196 38 621.6 0 38 197 63 621.6 0 63 198 42 621.6 0 42 199 30 621.6 0 30 200 40 621.6 0 40 201 31 621.6 0 31 202 32 621.6 0 32 203 36 621.6 0 36 204 41 621.6 0 41 205 37 621.6 0 37 206 42 621.6 0 42 207 46 621.6 0 46 208 37 621.6 0 37 209 37 621.6 0 37 210 30 621.6 0 30 211 46 621.6 0 46 212 154 621.6 0 154 213 610 621.6 0 610 214 127 621.6 0 127 215 39 621.6 0 39 216 54 621.6 0 54 217 59 621.6 0 59 218 188 621.6 0 188 219 170 621.6 0 170 220 133 621.6 0 133 221 47 621.6 0 47 222 36 621.6 0 36 223 42 621.6 0 42 224 32 621.6 0 32 225 44 621.6 0 44 226 47 621.6 0 47 227 27 621.6 0 27 228 36 621.6 0 36 229 33 621.6 0 33 230 44 621.6 0 44 231 33 621.6 0 33 232 66 621.6 0 66 233 46 621.6 0 46 234 56 621.6 0 56 235 80 621.6 0 80 236 112 621.6 0 112 237 106 621.6 0 106 238 119 621.6 0 119 239 70 621.6 0 70 240 36 621.6 0 36 241 71 621.6 0 71 242 58 621.6 0 58 243 235 621.6 0 235 244 214 621.6 0 214 245 243 621.6 0 243 246 111 621.6 0 111 247 96 621.6 0 96 248 75 621.6 0 75 249 67 621.6 0 67 250 71 621.6 0 71 251 51 621.6 0 51 252 49 621.6 0 49 253 36 621.6 0 36 254 61 621.6 0 61 255 70 621.6 0 70 256 219 621.6 0 219 257 174 621.6 0 174 258 174 621.6 0 174 259 141 621.6 0 141 260 983 621.6 0 983 261 754 621.6 0 754 262 760 621.6 0 760 263 150 621.6 0 150 264 62 621.6 0 62 265 67 621.6 0 67 266 54 621.6 0 54 267 64 621.6 0 64 268 69 621.6 0 69 269 60 621.6 0 60 270 71 621.6 0 71 271 75 621.6 0 75 272 130 621.6 0 130 273 234 621.6 0 234 274 93 621.6 0 93 275 118 621.6 0 118 276 202 621.6 0 202 277 103 621.6 0 103 278 125 621.6 0 125 279 199 621.6 0 199 280 226 621.6 0 226 281 216 621.6 0 216 282 224 621.6 0 224 283 266 621.6 0 266 284 367 621.6 0 367 285 439 621.6 0 439 286 7002 621.6 0 7002 287 6402 621.6 0 6402 288 9142 621.6 0 9142 289 101 621.6 0 101 290 55 621.6 0 55 291 35 621.6 0 35 292 45 621.6 0 45 293 54 621.6 0 54 294 54 621.6 0 54 295 51 621.6 0 51 296 126 621.6 0 126 297 136 621.6 0 136 298 283 621.6 0 283 299 178 621.6 0 178 300 59 621.6 0 59 301 31 621.6 0 31 Finished in 9.01 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 197,103 Reads with adapters: 83,181 (42.2%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 83,149 (42.2%) Total basepairs processed: 55,862,376 bp Total written (filtered): 22,857,378 bp (40.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 83181 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 12 0.0 3 0 0 10 2 16 414 0.0 3 0 405 6 3 17 78399 0.0 3 76725 1619 51 4 18 220 0.0 3 14 194 10 2 19 3930 0.0 3 3824 78 27 1 20 88 0.0 3 2 9 0 77 21 4 0.0 3 0 0 3 1 48 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 61 64 0.0 3 0 0 0 64 64 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 1 124 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 145 1 0.0 3 0 1 157 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 164 3 0.0 3 0 0 0 3 171 3 0.0 3 0 0 0 3 183 2 0.0 3 0 0 0 2 192 4 0.0 3 0 0 0 4 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.97 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 83,149 Reads with adapters: 3,247 (3.9%) Reads written (passing filters): 83,149 (100.0%) Total basepairs processed: 22,857,378 bp Total written (filtered): 22,847,539 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3247 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.6% C: 5.5% G: 31.0% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3162 1299.2 0 3162 4 79 324.8 0 79 5 5 81.2 0 5 12 1 0.0 1 0 1