This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.11 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 91,410 (3.6%) Reads that were too short: 29,420 (1.2%) Reads written (passing filters): 61,990 (2.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 14,974,305 bp (2.0%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 91410 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 318 621.6 0 318 7 928 621.6 0 928 8 16859 621.6 0 16859 9 14482 621.6 0 14482 10 105 621.6 0 105 11 105 621.6 0 105 12 93 621.6 0 93 13 81 621.6 0 81 14 90 621.6 0 90 15 88 621.6 0 88 16 111 621.6 0 111 17 82 621.6 0 82 18 78 621.6 0 78 19 66 621.6 0 66 20 88 621.6 0 88 21 104 621.6 0 104 22 94 621.6 0 94 23 103 621.6 0 103 24 85 621.6 0 85 25 104 621.6 0 104 26 82 621.6 0 82 27 72 621.6 0 72 28 109 621.6 0 109 29 90 621.6 0 90 30 87 621.6 0 87 31 79 621.6 0 79 32 101 621.6 0 101 33 84 621.6 0 84 34 97 621.6 0 97 35 83 621.6 0 83 36 93 621.6 0 93 37 98 621.6 0 98 38 193 621.6 0 193 39 106 621.6 0 106 40 161 621.6 0 161 41 103 621.6 0 103 42 95 621.6 0 95 43 87 621.6 0 87 44 68 621.6 0 68 45 78 621.6 0 78 46 99 621.6 0 99 47 90 621.6 0 90 48 103 621.6 0 103 49 109 621.6 0 109 50 86 621.6 0 86 51 107 621.6 0 107 52 126 621.6 0 126 53 115 621.6 0 115 54 104 621.6 0 104 55 96 621.6 0 96 56 100 621.6 0 100 57 94 621.6 0 94 58 109 621.6 0 109 59 70 621.6 0 70 60 123 621.6 0 123 61 111 621.6 0 111 62 124 621.6 0 124 63 120 621.6 0 120 64 106 621.6 0 106 65 109 621.6 0 109 66 134 621.6 0 134 67 136 621.6 0 136 68 128 621.6 0 128 69 111 621.6 0 111 70 111 621.6 0 111 71 127 621.6 0 127 72 114 621.6 0 114 73 88 621.6 0 88 74 131 621.6 0 131 75 136 621.6 0 136 76 121 621.6 0 121 77 110 621.6 0 110 78 159 621.6 0 159 79 100 621.6 0 100 80 125 621.6 0 125 81 92 621.6 0 92 82 128 621.6 0 128 83 115 621.6 0 115 84 137 621.6 0 137 85 102 621.6 0 102 86 92 621.6 0 92 87 127 621.6 0 127 88 126 621.6 0 126 89 95 621.6 0 95 90 121 621.6 0 121 91 193 621.6 0 193 92 120 621.6 0 120 93 144 621.6 0 144 94 139 621.6 0 139 95 211 621.6 0 211 96 186 621.6 0 186 97 769 621.6 0 769 98 1126 621.6 0 1126 99 963 621.6 0 963 100 401 621.6 0 401 101 103 621.6 0 103 102 80 621.6 0 80 103 94 621.6 0 94 104 100 621.6 0 100 105 116 621.6 0 116 106 124 621.6 0 124 107 138 621.6 0 138 108 159 621.6 0 159 109 186 621.6 0 186 110 151 621.6 0 151 111 557 621.6 0 557 112 211 621.6 0 211 113 453 621.6 0 453 114 86 621.6 0 86 115 109 621.6 0 109 116 78 621.6 0 78 117 110 621.6 0 110 118 103 621.6 0 103 119 116 621.6 0 116 120 111 621.6 0 111 121 92 621.6 0 92 122 119 621.6 0 119 123 89 621.6 0 89 124 90 621.6 0 90 125 89 621.6 0 89 126 113 621.6 0 113 127 97 621.6 0 97 128 166 621.6 0 166 129 139 621.6 0 139 130 163 621.6 0 163 131 147 621.6 0 147 132 353 621.6 0 353 133 167 621.6 0 167 134 251 621.6 0 251 135 116 621.6 0 116 136 135 621.6 0 135 137 95 621.6 0 95 138 117 621.6 0 117 139 84 621.6 0 84 140 112 621.6 0 112 141 151 621.6 0 151 142 103 621.6 0 103 143 92 621.6 0 92 144 109 621.6 0 109 145 122 621.6 0 122 146 147 621.6 0 147 147 152 621.6 0 152 148 131 621.6 0 131 149 117 621.6 0 117 150 103 621.6 0 103 151 93 621.6 0 93 152 114 621.6 0 114 153 146 621.6 0 146 154 442 621.6 0 442 155 544 621.6 0 544 156 624 621.6 0 624 157 197 621.6 0 197 158 107 621.6 0 107 159 105 621.6 0 105 160 91 621.6 0 91 161 147 621.6 0 147 162 110 621.6 0 110 163 90 621.6 0 90 164 113 621.6 0 113 165 145 621.6 0 145 166 678 621.6 0 678 167 254 621.6 0 254 168 267 621.6 0 267 169 91 621.6 0 91 170 126 621.6 0 126 171 91 621.6 0 91 172 109 621.6 0 109 173 93 621.6 0 93 174 102 621.6 0 102 175 106 621.6 0 106 176 115 621.6 0 115 177 111 621.6 0 111 178 121 621.6 0 121 179 131 621.6 0 131 180 157 621.6 0 157 181 196 621.6 0 196 182 106 621.6 0 106 183 108 621.6 0 108 184 120 621.6 0 120 185 132 621.6 0 132 186 119 621.6 0 119 187 106 621.6 0 106 188 127 621.6 0 127 189 120 621.6 0 120 190 105 621.6 0 105 191 93 621.6 0 93 192 128 621.6 0 128 193 380 621.6 0 380 194 310 621.6 0 310 195 293 621.6 0 293 196 353 621.6 0 353 197 151 621.6 0 151 198 161 621.6 0 161 199 168 621.6 0 168 200 367 621.6 0 367 201 232 621.6 0 232 202 261 621.6 0 261 203 99 621.6 0 99 204 103 621.6 0 103 205 115 621.6 0 115 206 136 621.6 0 136 207 135 621.6 0 135 208 108 621.6 0 108 209 94 621.6 0 94 210 90 621.6 0 90 211 86 621.6 0 86 212 109 621.6 0 109 213 95 621.6 0 95 214 130 621.6 0 130 215 132 621.6 0 132 216 132 621.6 0 132 217 180 621.6 0 180 218 140 621.6 0 140 219 142 621.6 0 142 220 629 621.6 0 629 221 607 621.6 0 607 222 595 621.6 0 595 223 221 621.6 0 221 224 122 621.6 0 122 225 98 621.6 0 98 226 109 621.6 0 109 227 136 621.6 0 136 228 116 621.6 0 116 229 119 621.6 0 119 230 103 621.6 0 103 231 123 621.6 0 123 232 115 621.6 0 115 233 106 621.6 0 106 234 92 621.6 0 92 235 136 621.6 0 136 236 152 621.6 0 152 237 345 621.6 0 345 238 609 621.6 0 609 239 432 621.6 0 432 240 229 621.6 0 229 241 226 621.6 0 226 242 255 621.6 0 255 243 586 621.6 0 586 244 425 621.6 0 425 245 443 621.6 0 443 246 199 621.6 0 199 247 126 621.6 0 126 248 167 621.6 0 167 249 193 621.6 0 193 250 142 621.6 0 142 251 174 621.6 0 174 252 184 621.6 0 184 253 201 621.6 0 201 254 1025 621.6 0 1025 255 602 621.6 0 602 256 1042 621.6 0 1042 257 143 621.6 0 143 258 161 621.6 0 161 259 198 621.6 0 198 260 233 621.6 0 233 261 378 621.6 0 378 262 4165 621.6 0 4165 263 1628 621.6 0 1628 264 2378 621.6 0 2378 265 213 621.6 0 213 266 116 621.6 0 116 267 98 621.6 0 98 268 103 621.6 0 103 269 128 621.6 0 128 270 154 621.6 0 154 271 146 621.6 0 146 272 118 621.6 0 118 273 114 621.6 0 114 274 344 621.6 0 344 275 294 621.6 0 294 276 254 621.6 0 254 277 134 621.6 0 134 278 129 621.6 0 129 279 175 621.6 0 175 280 189 621.6 0 189 281 252 621.6 0 252 282 365 621.6 0 365 283 267 621.6 0 267 284 153 621.6 0 153 285 149 621.6 0 149 286 122 621.6 0 122 287 122 621.6 0 122 288 160 621.6 0 160 289 246 621.6 0 246 290 192 621.6 0 192 291 192 621.6 0 192 292 141 621.6 0 141 293 126 621.6 0 126 294 172 621.6 0 172 295 457 621.6 0 457 296 142 621.6 0 142 297 136 621.6 0 136 298 101 621.6 0 101 299 129 621.6 0 129 300 173 621.6 0 173 301 159 621.6 0 159 Finished in 3.04 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 61,990 Reads with adapters: 15,327 (24.7%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 15,304 (24.7%) Total basepairs processed: 14,974,305 bp Total written (filtered): 4,156,969 bp (27.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15327 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 5 0.0 3 0 0 0 2 3 18 15 0.0 3 0 0 11 4 19 223 0.0 3 0 197 14 7 5 20 14900 0.0 4 13888 799 158 35 20 21 37 0.0 4 7 25 4 0 1 22 11 0.0 4 3 1 3 1 3 23 48 0.0 4 7 0 3 23 15 27 1 0.0 4 0 0 0 1 40 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 1 82 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 115 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 52 0.0 4 0 0 0 0 52 129 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 152 2 0.0 4 0 0 0 0 2 164 5 0.0 4 0 0 0 1 4 185 1 0.0 4 0 0 0 0 1 210 3 0.0 4 0 0 0 0 3 213 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 15,304 Reads with adapters: 3,876 (25.3%) Reads written (passing filters): 15,304 (100.0%) Total basepairs processed: 4,156,969 bp Total written (filtered): 4,116,513 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3876 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 9.2% G: 86.3% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 239.1 0 7 4 4 59.8 0 4 7 2 0.9 0 2 8 1 0.2 0 1 9 823 0.1 0 97 726 10 2627 0.0 1 170 2457 11 384 0.0 1 12 372 12 8 0.0 1 0 8 13 8 0.0 1 0 8 150 3 0.0 1 1 2 152 1 0.0 1 1 208 6 0.0 1 6 223 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.