This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.08 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 68,090 (2.7%) Reads that were too short: 18,040 (0.7%) Reads written (passing filters): 50,050 (2.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 12,948,868 bp (1.7%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 68090 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 228 621.6 0 228 7 708 621.6 0 708 8 15655 621.6 0 15655 9 17441 621.6 0 17441 10 79 621.6 0 79 11 51 621.6 0 51 12 39 621.6 0 39 13 65 621.6 0 65 14 51 621.6 0 51 15 49 621.6 0 49 16 44 621.6 0 44 17 38 621.6 0 38 18 36 621.6 0 36 19 46 621.6 0 46 20 59 621.6 0 59 21 57 621.6 0 57 22 63 621.6 0 63 23 60 621.6 0 60 24 60 621.6 0 60 25 52 621.6 0 52 26 53 621.6 0 53 27 42 621.6 0 42 28 64 621.6 0 64 29 54 621.6 0 54 30 51 621.6 0 51 31 63 621.6 0 63 32 66 621.6 0 66 33 52 621.6 0 52 34 67 621.6 0 67 35 49 621.6 0 49 36 61 621.6 0 61 37 66 621.6 0 66 38 135 621.6 0 135 39 69 621.6 0 69 40 83 621.6 0 83 41 70 621.6 0 70 42 54 621.6 0 54 43 57 621.6 0 57 44 53 621.6 0 53 45 46 621.6 0 46 46 54 621.6 0 54 47 47 621.6 0 47 48 60 621.6 0 60 49 48 621.6 0 48 50 51 621.6 0 51 51 73 621.6 0 73 52 64 621.6 0 64 53 57 621.6 0 57 54 65 621.6 0 65 55 55 621.6 0 55 56 71 621.6 0 71 57 42 621.6 0 42 58 55 621.6 0 55 59 43 621.6 0 43 60 91 621.6 0 91 61 70 621.6 0 70 62 55 621.6 0 55 63 80 621.6 0 80 64 73 621.6 0 73 65 61 621.6 0 61 66 81 621.6 0 81 67 75 621.6 0 75 68 63 621.6 0 63 69 68 621.6 0 68 70 67 621.6 0 67 71 78 621.6 0 78 72 71 621.6 0 71 73 74 621.6 0 74 74 89 621.6 0 89 75 81 621.6 0 81 76 45 621.6 0 45 77 60 621.6 0 60 78 82 621.6 0 82 79 42 621.6 0 42 80 56 621.6 0 56 81 73 621.6 0 73 82 80 621.6 0 80 83 55 621.6 0 55 84 90 621.6 0 90 85 80 621.6 0 80 86 72 621.6 0 72 87 63 621.6 0 63 88 81 621.6 0 81 89 81 621.6 0 81 90 39 621.6 0 39 91 130 621.6 0 130 92 76 621.6 0 76 93 77 621.6 0 77 94 74 621.6 0 74 95 149 621.6 0 149 96 90 621.6 0 90 97 283 621.6 0 283 98 358 621.6 0 358 99 285 621.6 0 285 100 160 621.6 0 160 101 66 621.6 0 66 102 52 621.6 0 52 103 58 621.6 0 58 104 76 621.6 0 76 105 79 621.6 0 79 106 63 621.6 0 63 107 77 621.6 0 77 108 79 621.6 0 79 109 91 621.6 0 91 110 87 621.6 0 87 111 494 621.6 0 494 112 175 621.6 0 175 113 283 621.6 0 283 114 63 621.6 0 63 115 65 621.6 0 65 116 47 621.6 0 47 117 58 621.6 0 58 118 72 621.6 0 72 119 82 621.6 0 82 120 75 621.6 0 75 121 55 621.6 0 55 122 78 621.6 0 78 123 59 621.6 0 59 124 66 621.6 0 66 125 74 621.6 0 74 126 58 621.6 0 58 127 74 621.6 0 74 128 108 621.6 0 108 129 84 621.6 0 84 130 75 621.6 0 75 131 76 621.6 0 76 132 134 621.6 0 134 133 68 621.6 0 68 134 102 621.6 0 102 135 69 621.6 0 69 136 80 621.6 0 80 137 71 621.6 0 71 138 70 621.6 0 70 139 52 621.6 0 52 140 62 621.6 0 62 141 83 621.6 0 83 142 66 621.6 0 66 143 57 621.6 0 57 144 57 621.6 0 57 145 79 621.6 0 79 146 76 621.6 0 76 147 58 621.6 0 58 148 77 621.6 0 77 149 63 621.6 0 63 150 66 621.6 0 66 151 66 621.6 0 66 152 75 621.6 0 75 153 84 621.6 0 84 154 195 621.6 0 195 155 249 621.6 0 249 156 220 621.6 0 220 157 121 621.6 0 121 158 64 621.6 0 64 159 60 621.6 0 60 160 55 621.6 0 55 161 118 621.6 0 118 162 56 621.6 0 56 163 52 621.6 0 52 164 104 621.6 0 104 165 69 621.6 0 69 166 238 621.6 0 238 167 91 621.6 0 91 168 110 621.6 0 110 169 65 621.6 0 65 170 61 621.6 0 61 171 46 621.6 0 46 172 66 621.6 0 66 173 52 621.6 0 52 174 77 621.6 0 77 175 61 621.6 0 61 176 65 621.6 0 65 177 69 621.6 0 69 178 64 621.6 0 64 179 87 621.6 0 87 180 122 621.6 0 122 181 135 621.6 0 135 182 72 621.6 0 72 183 61 621.6 0 61 184 68 621.6 0 68 185 55 621.6 0 55 186 75 621.6 0 75 187 68 621.6 0 68 188 54 621.6 0 54 189 71 621.6 0 71 190 70 621.6 0 70 191 70 621.6 0 70 192 57 621.6 0 57 193 140 621.6 0 140 194 221 621.6 0 221 195 123 621.6 0 123 196 246 621.6 0 246 197 67 621.6 0 67 198 82 621.6 0 82 199 92 621.6 0 92 200 131 621.6 0 131 201 112 621.6 0 112 202 107 621.6 0 107 203 53 621.6 0 53 204 54 621.6 0 54 205 51 621.6 0 51 206 76 621.6 0 76 207 84 621.6 0 84 208 78 621.6 0 78 209 64 621.6 0 64 210 74 621.6 0 74 211 59 621.6 0 59 212 61 621.6 0 61 213 83 621.6 0 83 214 86 621.6 0 86 215 66 621.6 0 66 216 66 621.6 0 66 217 81 621.6 0 81 218 73 621.6 0 73 219 91 621.6 0 91 220 370 621.6 0 370 221 259 621.6 0 259 222 243 621.6 0 243 223 120 621.6 0 120 224 83 621.6 0 83 225 63 621.6 0 63 226 66 621.6 0 66 227 83 621.6 0 83 228 76 621.6 0 76 229 84 621.6 0 84 230 76 621.6 0 76 231 61 621.6 0 61 232 62 621.6 0 62 233 60 621.6 0 60 234 75 621.6 0 75 235 57 621.6 0 57 236 89 621.6 0 89 237 316 621.6 0 316 238 614 621.6 0 614 239 366 621.6 0 366 240 84 621.6 0 84 241 139 621.6 0 139 242 117 621.6 0 117 243 318 621.6 0 318 244 191 621.6 0 191 245 199 621.6 0 199 246 98 621.6 0 98 247 77 621.6 0 77 248 83 621.6 0 83 249 139 621.6 0 139 250 100 621.6 0 100 251 105 621.6 0 105 252 117 621.6 0 117 253 120 621.6 0 120 254 344 621.6 0 344 255 195 621.6 0 195 256 416 621.6 0 416 257 106 621.6 0 106 258 110 621.6 0 110 259 112 621.6 0 112 260 137 621.6 0 137 261 254 621.6 0 254 262 3092 621.6 0 3092 263 1019 621.6 0 1019 264 1813 621.6 0 1813 265 128 621.6 0 128 266 72 621.6 0 72 267 63 621.6 0 63 268 65 621.6 0 65 269 78 621.6 0 78 270 79 621.6 0 79 271 75 621.6 0 75 272 79 621.6 0 79 273 79 621.6 0 79 274 151 621.6 0 151 275 113 621.6 0 113 276 146 621.6 0 146 277 84 621.6 0 84 278 57 621.6 0 57 279 97 621.6 0 97 280 105 621.6 0 105 281 116 621.6 0 116 282 135 621.6 0 135 283 111 621.6 0 111 284 89 621.6 0 89 285 76 621.6 0 76 286 99 621.6 0 99 287 76 621.6 0 76 288 89 621.6 0 89 289 145 621.6 0 145 290 108 621.6 0 108 291 108 621.6 0 108 292 92 621.6 0 92 293 119 621.6 0 119 294 126 621.6 0 126 295 574 621.6 0 574 296 81 621.6 0 81 297 71 621.6 0 71 298 69 621.6 0 69 299 86 621.6 0 86 300 102 621.6 0 102 301 82 621.6 0 82 Finished in 2.28 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 50,050 Reads with adapters: 15,614 (31.2%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 15,581 (31.1%) Total basepairs processed: 12,948,868 bp Total written (filtered): 4,297,504 bp (33.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15614 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 3 2 16 97 0.0 3 0 95 2 17 15383 0.0 3 15116 262 5 18 44 0.0 3 5 38 1 19 16 0.0 3 0 0 15 1 20 14 0.0 3 1 0 0 13 22 1 0.0 3 1 29 1 0.0 3 0 0 0 1 37 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 80 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 161 8 0.0 3 0 0 0 8 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 188 12 0.0 3 0 0 0 12 190 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 15,581 Reads with adapters: 104 (0.7%) Reads written (passing filters): 15,581 (100.0%) Total basepairs processed: 4,297,504 bp Total written (filtered): 4,297,131 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 104 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.5% C: 5.8% G: 56.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 43 243.5 0 43 4 61 60.9 0 61