This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.55 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 229,848 (9.0%) Reads that were too short: 16,762 (0.7%) Reads written (passing filters): 213,086 (8.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 49,081,274 bp (6.4%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 229848 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 621.6 0 402 7 5829 621.6 0 5829 8 6892 621.6 0 6892 9 141 621.6 0 141 10 154 621.6 0 154 11 133 621.6 0 133 12 203 621.6 0 203 13 136 621.6 0 136 14 108 621.6 0 108 15 162 621.6 0 162 16 127 621.6 0 127 17 160 621.6 0 160 18 166 621.6 0 166 19 203 621.6 0 203 20 165 621.6 0 165 21 179 621.6 0 179 22 157 621.6 0 157 23 155 621.6 0 155 24 173 621.6 0 173 25 158 621.6 0 158 26 177 621.6 0 177 27 121 621.6 0 121 28 137 621.6 0 137 29 408 621.6 0 408 30 143 621.6 0 143 31 108 621.6 0 108 32 121 621.6 0 121 33 130 621.6 0 130 34 127 621.6 0 127 35 130 621.6 0 130 36 185 621.6 0 185 37 157 621.6 0 157 38 246 621.6 0 246 39 455 621.6 0 455 40 214 621.6 0 214 41 222 621.6 0 222 42 192 621.6 0 192 43 241 621.6 0 241 44 414 621.6 0 414 45 226 621.6 0 226 46 220 621.6 0 220 47 191 621.6 0 191 48 174 621.6 0 174 49 283 621.6 0 283 50 357 621.6 0 357 51 599 621.6 0 599 52 796 621.6 0 796 53 954 621.6 0 954 54 2633 621.6 0 2633 55 5916 621.6 0 5916 56 80439 621.6 0 80439 57 22957 621.6 0 22957 58 37343 621.6 0 37343 59 269 621.6 0 269 60 165 621.6 0 165 61 125 621.6 0 125 62 163 621.6 0 163 63 149 621.6 0 149 64 150 621.6 0 150 65 112 621.6 0 112 66 130 621.6 0 130 67 132 621.6 0 132 68 141 621.6 0 141 69 133 621.6 0 133 70 149 621.6 0 149 71 137 621.6 0 137 72 134 621.6 0 134 73 130 621.6 0 130 74 138 621.6 0 138 75 143 621.6 0 143 76 105 621.6 0 105 77 114 621.6 0 114 78 108 621.6 0 108 79 100 621.6 0 100 80 116 621.6 0 116 81 138 621.6 0 138 82 103 621.6 0 103 83 170 621.6 0 170 84 139 621.6 0 139 85 112 621.6 0 112 86 109 621.6 0 109 87 104 621.6 0 104 88 99 621.6 0 99 89 96 621.6 0 96 90 130 621.6 0 130 91 76 621.6 0 76 92 99 621.6 0 99 93 92 621.6 0 92 94 101 621.6 0 101 95 70 621.6 0 70 96 126 621.6 0 126 97 95 621.6 0 95 98 99 621.6 0 99 99 120 621.6 0 120 100 124 621.6 0 124 101 108 621.6 0 108 102 97 621.6 0 97 103 183 621.6 0 183 104 175 621.6 0 175 105 182 621.6 0 182 106 97 621.6 0 97 107 103 621.6 0 103 108 119 621.6 0 119 109 133 621.6 0 133 110 108 621.6 0 108 111 116 621.6 0 116 112 86 621.6 0 86 113 105 621.6 0 105 114 128 621.6 0 128 115 105 621.6 0 105 116 111 621.6 0 111 117 128 621.6 0 128 118 109 621.6 0 109 119 139 621.6 0 139 120 148 621.6 0 148 121 111 621.6 0 111 122 97 621.6 0 97 123 123 621.6 0 123 124 465 621.6 0 465 125 1452 621.6 0 1452 126 903 621.6 0 903 127 150 621.6 0 150 128 106 621.6 0 106 129 124 621.6 0 124 130 155 621.6 0 155 131 142 621.6 0 142 132 137 621.6 0 137 133 126 621.6 0 126 134 109 621.6 0 109 135 149 621.6 0 149 136 127 621.6 0 127 137 117 621.6 0 117 138 138 621.6 0 138 139 138 621.6 0 138 140 122 621.6 0 122 141 151 621.6 0 151 142 124 621.6 0 124 143 144 621.6 0 144 144 166 621.6 0 166 145 171 621.6 0 171 146 242 621.6 0 242 147 870 621.6 0 870 148 603 621.6 0 603 149 1626 621.6 0 1626 150 758 621.6 0 758 151 326 621.6 0 326 152 458 621.6 0 458 153 135 621.6 0 135 154 189 621.6 0 189 155 181 621.6 0 181 156 132 621.6 0 132 157 156 621.6 0 156 158 109 621.6 0 109 159 137 621.6 0 137 160 206 621.6 0 206 161 197 621.6 0 197 162 420 621.6 0 420 163 165 621.6 0 165 164 134 621.6 0 134 165 237 621.6 0 237 166 399 621.6 0 399 167 2676 621.6 0 2676 168 2633 621.6 0 2633 169 1416 621.6 0 1416 170 145 621.6 0 145 171 149 621.6 0 149 172 186 621.6 0 186 173 174 621.6 0 174 174 145 621.6 0 145 175 309 621.6 0 309 176 245 621.6 0 245 177 355 621.6 0 355 178 122 621.6 0 122 179 113 621.6 0 113 180 169 621.6 0 169 181 109 621.6 0 109 182 98 621.6 0 98 183 134 621.6 0 134 184 106 621.6 0 106 185 92 621.6 0 92 186 150 621.6 0 150 187 119 621.6 0 119 188 141 621.6 0 141 189 157 621.6 0 157 190 114 621.6 0 114 191 98 621.6 0 98 192 122 621.6 0 122 193 130 621.6 0 130 194 158 621.6 0 158 195 408 621.6 0 408 196 458 621.6 0 458 197 297 621.6 0 297 198 270 621.6 0 270 199 549 621.6 0 549 200 4622 621.6 0 4622 201 1946 621.6 0 1946 202 2612 621.6 0 2612 203 181 621.6 0 181 204 102 621.6 0 102 205 107 621.6 0 107 206 103 621.6 0 103 207 128 621.6 0 128 208 140 621.6 0 140 209 108 621.6 0 108 210 94 621.6 0 94 211 106 621.6 0 106 212 121 621.6 0 121 213 118 621.6 0 118 214 122 621.6 0 122 215 106 621.6 0 106 216 114 621.6 0 114 217 133 621.6 0 133 218 105 621.6 0 105 219 135 621.6 0 135 220 160 621.6 0 160 221 156 621.6 0 156 222 120 621.6 0 120 223 79 621.6 0 79 224 106 621.6 0 106 225 107 621.6 0 107 226 112 621.6 0 112 227 106 621.6 0 106 228 140 621.6 0 140 229 131 621.6 0 131 230 270 621.6 0 270 231 339 621.6 0 339 232 313 621.6 0 313 233 201 621.6 0 201 234 235 621.6 0 235 235 105 621.6 0 105 236 98 621.6 0 98 237 104 621.6 0 104 238 126 621.6 0 126 239 137 621.6 0 137 240 129 621.6 0 129 241 148 621.6 0 148 242 132 621.6 0 132 243 140 621.6 0 140 244 134 621.6 0 134 245 93 621.6 0 93 246 129 621.6 0 129 247 103 621.6 0 103 248 134 621.6 0 134 249 122 621.6 0 122 250 133 621.6 0 133 251 96 621.6 0 96 252 151 621.6 0 151 253 115 621.6 0 115 254 103 621.6 0 103 255 117 621.6 0 117 256 110 621.6 0 110 257 85 621.6 0 85 258 122 621.6 0 122 259 89 621.6 0 89 260 115 621.6 0 115 261 116 621.6 0 116 262 124 621.6 0 124 263 104 621.6 0 104 264 108 621.6 0 108 265 131 621.6 0 131 266 102 621.6 0 102 267 115 621.6 0 115 268 193 621.6 0 193 269 179 621.6 0 179 270 169 621.6 0 169 271 128 621.6 0 128 272 136 621.6 0 136 273 163 621.6 0 163 274 128 621.6 0 128 275 142 621.6 0 142 276 242 621.6 0 242 277 155 621.6 0 155 278 210 621.6 0 210 279 145 621.6 0 145 280 154 621.6 0 154 281 237 621.6 0 237 282 253 621.6 0 253 283 412 621.6 0 412 284 106 621.6 0 106 285 100 621.6 0 100 286 114 621.6 0 114 287 124 621.6 0 124 288 122 621.6 0 122 289 181 621.6 0 181 290 161 621.6 0 161 291 135 621.6 0 135 292 110 621.6 0 110 293 157 621.6 0 157 294 143 621.6 0 143 295 164 621.6 0 164 296 166 621.6 0 166 297 120 621.6 0 120 298 107 621.6 0 107 299 140 621.6 0 140 300 235 621.6 0 235 301 251 621.6 0 251 Finished in 8.24 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 213,086 Reads with adapters: 5,731 (2.7%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 5,695 (2.7%) Total basepairs processed: 49,081,274 bp Total written (filtered): 1,569,635 bp (3.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5731 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 30 0.0 3 0 30 17 5594 0.0 3 5478 113 3 18 13 0.0 3 0 12 0 1 19 4 0.0 3 0 0 4 20 5 0.0 3 0 0 0 5 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 53 24 0.0 3 0 0 1 23 56 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 92 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 121 1 0.0 3 1 122 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 3 0.0 3 0 0 0 3 146 1 0.0 3 0 1 165 6 0.0 3 0 0 0 6 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 184 1 0.0 3 1 193 1 0.0 3 0 0 0 1 195 1 0.0 3 1 199 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 231 3 0.0 3 0 0 0 3 232 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 1 247 1 0.0 3 0 0 0 1 252 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 5,695 Reads with adapters: 18 (0.3%) Reads written (passing filters): 5,695 (100.0%) Total basepairs processed: 1,569,635 bp Total written (filtered): 1,569,581 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 18 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.8% C: 38.9% G: 11.1% T: 22.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18 89.0 0 18