This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.81 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 335,562 (13.2%) Reads that were too short: 14,466 (0.6%) Reads written (passing filters): 321,096 (12.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 76,421,124 bp (10.0%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 335562 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 887 621.6 0 887 7 11712 621.6 0 11712 8 247 621.6 0 247 9 10661 621.6 0 10661 10 126 621.6 0 126 11 115 621.6 0 115 12 168 621.6 0 168 13 166 621.6 0 166 14 130 621.6 0 130 15 147 621.6 0 147 16 168 621.6 0 168 17 180 621.6 0 180 18 170 621.6 0 170 19 153 621.6 0 153 20 200 621.6 0 200 21 185 621.6 0 185 22 144 621.6 0 144 23 165 621.6 0 165 24 185 621.6 0 185 25 186 621.6 0 186 26 200 621.6 0 200 27 212 621.6 0 212 28 245 621.6 0 245 29 319 621.6 0 319 30 290 621.6 0 290 31 269 621.6 0 269 32 295 621.6 0 295 33 227 621.6 0 227 34 266 621.6 0 266 35 438 621.6 0 438 36 256 621.6 0 256 37 252 621.6 0 252 38 1014 621.6 0 1014 39 279 621.6 0 279 40 283 621.6 0 283 41 315 621.6 0 315 42 3976 621.6 0 3976 43 2678 621.6 0 2678 44 6835 621.6 0 6835 45 279 621.6 0 279 46 237 621.6 0 237 47 501 621.6 0 501 48 1249 621.6 0 1249 49 267 621.6 0 267 50 457 621.6 0 457 51 320 621.6 0 320 52 356 621.6 0 356 53 1205 621.6 0 1205 54 749 621.6 0 749 55 700 621.6 0 700 56 248 621.6 0 248 57 317 621.6 0 317 58 463 621.6 0 463 59 509 621.6 0 509 60 841 621.6 0 841 61 1359 621.6 0 1359 62 1227 621.6 0 1227 63 3024 621.6 0 3024 64 7836 621.6 0 7836 65 71128 621.6 0 71128 66 83292 621.6 0 83292 67 81672 621.6 0 81672 68 521 621.6 0 521 69 178 621.6 0 178 70 165 621.6 0 165 71 153 621.6 0 153 72 150 621.6 0 150 73 185 621.6 0 185 74 114 621.6 0 114 75 138 621.6 0 138 76 145 621.6 0 145 77 170 621.6 0 170 78 112 621.6 0 112 79 149 621.6 0 149 80 159 621.6 0 159 81 148 621.6 0 148 82 147 621.6 0 147 83 138 621.6 0 138 84 152 621.6 0 152 85 141 621.6 0 141 86 131 621.6 0 131 87 144 621.6 0 144 88 142 621.6 0 142 89 189 621.6 0 189 90 134 621.6 0 134 91 164 621.6 0 164 92 134 621.6 0 134 93 136 621.6 0 136 94 158 621.6 0 158 95 197 621.6 0 197 96 170 621.6 0 170 97 119 621.6 0 119 98 151 621.6 0 151 99 168 621.6 0 168 100 155 621.6 0 155 101 152 621.6 0 152 102 183 621.6 0 183 103 167 621.6 0 167 104 136 621.6 0 136 105 162 621.6 0 162 106 126 621.6 0 126 107 152 621.6 0 152 108 156 621.6 0 156 109 132 621.6 0 132 110 170 621.6 0 170 111 148 621.6 0 148 112 147 621.6 0 147 113 145 621.6 0 145 114 121 621.6 0 121 115 114 621.6 0 114 116 116 621.6 0 116 117 122 621.6 0 122 118 133 621.6 0 133 119 115 621.6 0 115 120 98 621.6 0 98 121 144 621.6 0 144 122 97 621.6 0 97 123 143 621.6 0 143 124 153 621.6 0 153 125 134 621.6 0 134 126 159 621.6 0 159 127 143 621.6 0 143 128 118 621.6 0 118 129 135 621.6 0 135 130 165 621.6 0 165 131 120 621.6 0 120 132 147 621.6 0 147 133 158 621.6 0 158 134 274 621.6 0 274 135 309 621.6 0 309 136 189 621.6 0 189 137 104 621.6 0 104 138 131 621.6 0 131 139 147 621.6 0 147 140 132 621.6 0 132 141 118 621.6 0 118 142 116 621.6 0 116 143 140 621.6 0 140 144 94 621.6 0 94 145 103 621.6 0 103 146 123 621.6 0 123 147 114 621.6 0 114 148 127 621.6 0 127 149 107 621.6 0 107 150 63 621.6 0 63 151 96 621.6 0 96 152 110 621.6 0 110 153 99 621.6 0 99 154 114 621.6 0 114 155 95 621.6 0 95 156 96 621.6 0 96 157 105 621.6 0 105 158 93 621.6 0 93 159 113 621.6 0 113 160 118 621.6 0 118 161 127 621.6 0 127 162 136 621.6 0 136 163 103 621.6 0 103 164 113 621.6 0 113 165 116 621.6 0 116 166 114 621.6 0 114 167 113 621.6 0 113 168 136 621.6 0 136 169 119 621.6 0 119 170 102 621.6 0 102 171 97 621.6 0 97 172 103 621.6 0 103 173 104 621.6 0 104 174 140 621.6 0 140 175 112 621.6 0 112 176 101 621.6 0 101 177 108 621.6 0 108 178 141 621.6 0 141 179 138 621.6 0 138 180 125 621.6 0 125 181 113 621.6 0 113 182 93 621.6 0 93 183 94 621.6 0 94 184 115 621.6 0 115 185 133 621.6 0 133 186 129 621.6 0 129 187 100 621.6 0 100 188 97 621.6 0 97 189 114 621.6 0 114 190 121 621.6 0 121 191 131 621.6 0 131 192 84 621.6 0 84 193 88 621.6 0 88 194 125 621.6 0 125 195 114 621.6 0 114 196 86 621.6 0 86 197 148 621.6 0 148 198 128 621.6 0 128 199 104 621.6 0 104 200 146 621.6 0 146 201 107 621.6 0 107 202 112 621.6 0 112 203 119 621.6 0 119 204 128 621.6 0 128 205 110 621.6 0 110 206 106 621.6 0 106 207 108 621.6 0 108 208 84 621.6 0 84 209 139 621.6 0 139 210 110 621.6 0 110 211 108 621.6 0 108 212 86 621.6 0 86 213 114 621.6 0 114 214 104 621.6 0 104 215 100 621.6 0 100 216 106 621.6 0 106 217 136 621.6 0 136 218 106 621.6 0 106 219 121 621.6 0 121 220 146 621.6 0 146 221 108 621.6 0 108 222 347 621.6 0 347 223 272 621.6 0 272 224 335 621.6 0 335 225 157 621.6 0 157 226 99 621.6 0 99 227 118 621.6 0 118 228 104 621.6 0 104 229 101 621.6 0 101 230 110 621.6 0 110 231 91 621.6 0 91 232 84 621.6 0 84 233 103 621.6 0 103 234 104 621.6 0 104 235 125 621.6 0 125 236 138 621.6 0 138 237 100 621.6 0 100 238 121 621.6 0 121 239 85 621.6 0 85 240 122 621.6 0 122 241 99 621.6 0 99 242 235 621.6 0 235 243 245 621.6 0 245 244 188 621.6 0 188 245 155 621.6 0 155 246 131 621.6 0 131 247 110 621.6 0 110 248 88 621.6 0 88 249 100 621.6 0 100 250 116 621.6 0 116 251 107 621.6 0 107 252 150 621.6 0 150 253 173 621.6 0 173 254 205 621.6 0 205 255 438 621.6 0 438 256 314 621.6 0 314 257 723 621.6 0 723 258 136 621.6 0 136 259 138 621.6 0 138 260 361 621.6 0 361 261 468 621.6 0 468 262 738 621.6 0 738 263 136 621.6 0 136 264 100 621.6 0 100 265 103 621.6 0 103 266 152 621.6 0 152 267 100 621.6 0 100 268 67 621.6 0 67 269 130 621.6 0 130 270 239 621.6 0 239 271 136 621.6 0 136 272 163 621.6 0 163 273 85 621.6 0 85 274 107 621.6 0 107 275 86 621.6 0 86 276 85 621.6 0 85 277 121 621.6 0 121 278 101 621.6 0 101 279 114 621.6 0 114 280 96 621.6 0 96 281 98 621.6 0 98 282 79 621.6 0 79 283 65 621.6 0 65 284 97 621.6 0 97 285 76 621.6 0 76 286 95 621.6 0 95 287 79 621.6 0 79 288 101 621.6 0 101 289 82 621.6 0 82 290 98 621.6 0 98 291 103 621.6 0 103 292 111 621.6 0 111 293 92 621.6 0 92 294 69 621.6 0 69 295 71 621.6 0 71 296 72 621.6 0 72 297 105 621.6 0 105 298 92 621.6 0 92 299 128 621.6 0 128 300 118 621.6 0 118 301 129 621.6 0 129 Finished in 12.44 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 321,096 Reads with adapters: 10,980 (3.4%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 10,957 (3.4%) Total basepairs processed: 76,421,124 bp Total written (filtered): 3,011,194 bp (3.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10980 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 102 0.0 3 0 99 3 17 10605 0.0 3 10398 203 4 18 24 0.0 3 2 21 1 19 8 0.0 3 1 3 3 1 20 197 0.0 3 184 3 0 10 21 2 0.0 3 1 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 74 1 0.0 3 1 92 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 117 1 0.0 3 1 118 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 197 1 0.0 3 0 1 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 223 4 0.0 3 0 0 0 4 224 2 0.0 3 0 0 0 2 233 1 0.0 3 1 244 1 0.0 3 1 253 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 10,957 Reads with adapters: 452 (4.1%) Reads written (passing filters): 10,957 (100.0%) Total basepairs processed: 3,011,194 bp Total written (filtered): 3,009,835 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 452 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.9% C: 6.2% G: 6.2% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 450 171.2 0 450 4 1 42.8 0 1 5 1 10.7 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.