This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.35 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 142,675 (5.6%) Reads that were too short: 24,246 (1.0%) Reads written (passing filters): 118,429 (4.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,598,290 bp (4.1%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 142675 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2776 621.6 0 2776 7 1087 621.6 0 1087 8 46997 621.6 0 46997 9 38510 621.6 0 38510 10 112 621.6 0 112 11 84 621.6 0 84 12 77 621.6 0 77 13 89 621.6 0 89 14 67 621.6 0 67 15 76 621.6 0 76 16 106 621.6 0 106 17 108 621.6 0 108 18 89 621.6 0 89 19 93 621.6 0 93 20 88 621.6 0 88 21 64 621.6 0 64 22 119 621.6 0 119 23 108 621.6 0 108 24 225 621.6 0 225 25 151 621.6 0 151 26 132 621.6 0 132 27 86 621.6 0 86 28 99 621.6 0 99 29 139 621.6 0 139 30 108 621.6 0 108 31 95 621.6 0 95 32 62 621.6 0 62 33 70 621.6 0 70 34 109 621.6 0 109 35 116 621.6 0 116 36 156 621.6 0 156 37 87 621.6 0 87 38 91 621.6 0 91 39 80 621.6 0 80 40 83 621.6 0 83 41 81 621.6 0 81 42 95 621.6 0 95 43 103 621.6 0 103 44 80 621.6 0 80 45 86 621.6 0 86 46 87 621.6 0 87 47 78 621.6 0 78 48 78 621.6 0 78 49 99 621.6 0 99 50 91 621.6 0 91 51 78 621.6 0 78 52 97 621.6 0 97 53 107 621.6 0 107 54 104 621.6 0 104 55 75 621.6 0 75 56 83 621.6 0 83 57 99 621.6 0 99 58 116 621.6 0 116 59 61 621.6 0 61 60 66 621.6 0 66 61 69 621.6 0 69 62 75 621.6 0 75 63 128 621.6 0 128 64 101 621.6 0 101 65 120 621.6 0 120 66 105 621.6 0 105 67 159 621.6 0 159 68 134 621.6 0 134 69 298 621.6 0 298 70 111 621.6 0 111 71 174 621.6 0 174 72 108 621.6 0 108 73 98 621.6 0 98 74 99 621.6 0 99 75 101 621.6 0 101 76 202 621.6 0 202 77 202 621.6 0 202 78 249 621.6 0 249 79 198 621.6 0 198 80 581 621.6 0 581 81 518 621.6 0 518 82 470 621.6 0 470 83 116 621.6 0 116 84 97 621.6 0 97 85 117 621.6 0 117 86 130 621.6 0 130 87 105 621.6 0 105 88 80 621.6 0 80 89 109 621.6 0 109 90 104 621.6 0 104 91 105 621.6 0 105 92 96 621.6 0 96 93 133 621.6 0 133 94 113 621.6 0 113 95 156 621.6 0 156 96 1344 621.6 0 1344 97 389 621.6 0 389 98 1081 621.6 0 1081 99 147 621.6 0 147 100 126 621.6 0 126 101 106 621.6 0 106 102 114 621.6 0 114 103 86 621.6 0 86 104 91 621.6 0 91 105 107 621.6 0 107 106 91 621.6 0 91 107 100 621.6 0 100 108 180 621.6 0 180 109 110 621.6 0 110 110 121 621.6 0 121 111 109 621.6 0 109 112 110 621.6 0 110 113 125 621.6 0 125 114 135 621.6 0 135 115 112 621.6 0 112 116 90 621.6 0 90 117 100 621.6 0 100 118 81 621.6 0 81 119 88 621.6 0 88 120 105 621.6 0 105 121 80 621.6 0 80 122 102 621.6 0 102 123 93 621.6 0 93 124 103 621.6 0 103 125 109 621.6 0 109 126 110 621.6 0 110 127 168 621.6 0 168 128 158 621.6 0 158 129 180 621.6 0 180 130 241 621.6 0 241 131 188 621.6 0 188 132 403 621.6 0 403 133 204 621.6 0 204 134 336 621.6 0 336 135 128 621.6 0 128 136 109 621.6 0 109 137 139 621.6 0 139 138 123 621.6 0 123 139 113 621.6 0 113 140 126 621.6 0 126 141 103 621.6 0 103 142 91 621.6 0 91 143 124 621.6 0 124 144 110 621.6 0 110 145 87 621.6 0 87 146 103 621.6 0 103 147 123 621.6 0 123 148 257 621.6 0 257 149 156 621.6 0 156 150 821 621.6 0 821 151 93 621.6 0 93 152 82 621.6 0 82 153 104 621.6 0 104 154 80 621.6 0 80 155 74 621.6 0 74 156 107 621.6 0 107 157 84 621.6 0 84 158 108 621.6 0 108 159 95 621.6 0 95 160 120 621.6 0 120 161 136 621.6 0 136 162 119 621.6 0 119 163 87 621.6 0 87 164 98 621.6 0 98 165 92 621.6 0 92 166 98 621.6 0 98 167 107 621.6 0 107 168 103 621.6 0 103 169 97 621.6 0 97 170 101 621.6 0 101 171 83 621.6 0 83 172 101 621.6 0 101 173 96 621.6 0 96 174 86 621.6 0 86 175 85 621.6 0 85 176 116 621.6 0 116 177 87 621.6 0 87 178 90 621.6 0 90 179 87 621.6 0 87 180 97 621.6 0 97 181 133 621.6 0 133 182 95 621.6 0 95 183 134 621.6 0 134 184 130 621.6 0 130 185 182 621.6 0 182 186 210 621.6 0 210 187 1351 621.6 0 1351 188 604 621.6 0 604 189 482 621.6 0 482 190 611 621.6 0 611 191 94 621.6 0 94 192 100 621.6 0 100 193 80 621.6 0 80 194 88 621.6 0 88 195 103 621.6 0 103 196 127 621.6 0 127 197 103 621.6 0 103 198 109 621.6 0 109 199 94 621.6 0 94 200 86 621.6 0 86 201 100 621.6 0 100 202 92 621.6 0 92 203 120 621.6 0 120 204 96 621.6 0 96 205 124 621.6 0 124 206 97 621.6 0 97 207 144 621.6 0 144 208 125 621.6 0 125 209 466 621.6 0 466 210 734 621.6 0 734 211 519 621.6 0 519 212 246 621.6 0 246 213 120 621.6 0 120 214 119 621.6 0 119 215 79 621.6 0 79 216 86 621.6 0 86 217 97 621.6 0 97 218 78 621.6 0 78 219 127 621.6 0 127 220 106 621.6 0 106 221 106 621.6 0 106 222 96 621.6 0 96 223 214 621.6 0 214 224 172 621.6 0 172 225 163 621.6 0 163 226 251 621.6 0 251 227 295 621.6 0 295 228 209 621.6 0 209 229 110 621.6 0 110 230 138 621.6 0 138 231 127 621.6 0 127 232 146 621.6 0 146 233 169 621.6 0 169 234 171 621.6 0 171 235 227 621.6 0 227 236 190 621.6 0 190 237 144 621.6 0 144 238 142 621.6 0 142 239 154 621.6 0 154 240 612 621.6 0 612 241 466 621.6 0 466 242 719 621.6 0 719 243 313 621.6 0 313 244 408 621.6 0 408 245 199 621.6 0 199 246 483 621.6 0 483 247 288 621.6 0 288 248 793 621.6 0 793 249 761 621.6 0 761 250 407 621.6 0 407 251 540 621.6 0 540 252 210 621.6 0 210 253 679 621.6 0 679 254 855 621.6 0 855 255 648 621.6 0 648 256 250 621.6 0 250 257 165 621.6 0 165 258 241 621.6 0 241 259 159 621.6 0 159 260 479 621.6 0 479 261 651 621.6 0 651 262 365 621.6 0 365 263 159 621.6 0 159 264 89 621.6 0 89 265 343 621.6 0 343 266 236 621.6 0 236 267 246 621.6 0 246 268 206 621.6 0 206 269 528 621.6 0 528 270 658 621.6 0 658 271 388 621.6 0 388 272 391 621.6 0 391 273 409 621.6 0 409 274 329 621.6 0 329 275 109 621.6 0 109 276 80 621.6 0 80 277 74 621.6 0 74 278 80 621.6 0 80 279 103 621.6 0 103 280 109 621.6 0 109 281 130 621.6 0 130 282 107 621.6 0 107 283 158 621.6 0 158 284 155 621.6 0 155 285 140 621.6 0 140 286 83 621.6 0 83 287 71 621.6 0 71 288 62 621.6 0 62 289 67 621.6 0 67 290 100 621.6 0 100 291 77 621.6 0 77 292 99 621.6 0 99 293 107 621.6 0 107 294 144 621.6 0 144 295 100 621.6 0 100 296 112 621.6 0 112 297 75 621.6 0 75 298 101 621.6 0 101 299 90 621.6 0 90 300 111 621.6 0 111 301 130 621.6 0 130 Finished in 6.14 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 118,429 Reads with adapters: 40,100 (33.9%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 40,036 (33.8%) Total basepairs processed: 31,598,290 bp Total written (filtered): 10,874,461 bp (34.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 40100 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 6 0.0 3 0 0 0 2 4 18 23 0.0 3 0 0 12 7 4 19 565 0.0 3 0 480 46 25 14 20 39071 0.0 4 36391 2125 399 116 40 21 92 0.0 4 9 69 9 3 2 22 8 0.0 4 0 0 4 1 3 23 106 0.0 4 0 0 0 78 28 24 1 0.0 4 1 27 2 0.0 4 1 0 0 0 1 34 1 0.0 4 1 37 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 1 50 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 7 0.0 4 0 0 0 0 7 58 1 0.0 4 0 0 0 0 1 87 5 0.0 4 0 0 0 0 5 88 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 129 0.0 4 0 0 0 0 129 128 4 0.0 4 0 0 0 0 4 129 2 0.0 4 0 0 0 0 2 130 4 0.0 4 0 0 0 0 4 140 1 0.0 4 0 0 0 0 1 149 5 0.0 4 0 0 0 1 4 153 3 0.0 4 0 0 0 0 3 163 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 185 1 0.0 4 1 195 2 0.0 4 0 0 0 0 2 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 5 0.0 4 0 0 0 0 5 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 1 1 237 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 1 261 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.46 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 40,036 Reads with adapters: 9,415 (23.5%) Reads written (passing filters): 40,036 (100.0%) Total basepairs processed: 10,874,461 bp Total written (filtered): 10,765,301 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9415 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 10.1% G: 84.5% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 28 625.6 0 28 4 9 156.4 0 9 6 3 9.8 0 3 8 3 0.6 0 3 9 1573 0.2 0 160 1413 10 6192 0.0 1 450 5742 11 1458 0.0 1 61 1397 12 28 0.0 1 0 28 13 25 0.0 1 0 25 14 2 0.0 1 0 2 19 5 0.0 1 3 2 20 1 0.0 1 0 1 22 2 0.0 1 2 72 1 0.0 1 1 138 1 0.0 1 1 145 2 0.0 1 2 149 1 0.0 1 0 1 150 23 0.0 1 21 2 152 3 0.0 1 1 2 202 1 0.0 1 1 208 46 0.0 1 38 8 209 1 0.0 1 0 1 210 5 0.0 1 3 2 223 1 0.0 1 1 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.