This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.41 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 129,918 (5.1%) Reads that were too short: 15,642 (0.6%) Reads written (passing filters): 114,276 (4.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,094,374 bp (4.1%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 129918 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2429 621.6 0 2429 7 887 621.6 0 887 8 42452 621.6 0 42452 9 46766 621.6 0 46766 10 97 621.6 0 97 11 68 621.6 0 68 12 70 621.6 0 70 13 59 621.6 0 59 14 45 621.6 0 45 15 49 621.6 0 49 16 66 621.6 0 66 17 53 621.6 0 53 18 64 621.6 0 64 19 68 621.6 0 68 20 78 621.6 0 78 21 47 621.6 0 47 22 57 621.6 0 57 23 52 621.6 0 52 24 71 621.6 0 71 25 53 621.6 0 53 26 63 621.6 0 63 27 69 621.6 0 69 28 77 621.6 0 77 29 90 621.6 0 90 30 63 621.6 0 63 31 49 621.6 0 49 32 31 621.6 0 31 33 50 621.6 0 50 34 73 621.6 0 73 35 64 621.6 0 64 36 79 621.6 0 79 37 64 621.6 0 64 38 83 621.6 0 83 39 76 621.6 0 76 40 68 621.6 0 68 41 51 621.6 0 51 42 75 621.6 0 75 43 73 621.6 0 73 44 71 621.6 0 71 45 56 621.6 0 56 46 79 621.6 0 79 47 67 621.6 0 67 48 66 621.6 0 66 49 75 621.6 0 75 50 53 621.6 0 53 51 90 621.6 0 90 52 60 621.6 0 60 53 72 621.6 0 72 54 66 621.6 0 66 55 59 621.6 0 59 56 49 621.6 0 49 57 63 621.6 0 63 58 79 621.6 0 79 59 50 621.6 0 50 60 53 621.6 0 53 61 57 621.6 0 57 62 57 621.6 0 57 63 76 621.6 0 76 64 79 621.6 0 79 65 87 621.6 0 87 66 68 621.6 0 68 67 94 621.6 0 94 68 106 621.6 0 106 69 303 621.6 0 303 70 75 621.6 0 75 71 122 621.6 0 122 72 73 621.6 0 73 73 65 621.6 0 65 74 43 621.6 0 43 75 70 621.6 0 70 76 129 621.6 0 129 77 140 621.6 0 140 78 139 621.6 0 139 79 114 621.6 0 114 80 526 621.6 0 526 81 502 621.6 0 502 82 467 621.6 0 467 83 73 621.6 0 73 84 68 621.6 0 68 85 96 621.6 0 96 86 83 621.6 0 83 87 66 621.6 0 66 88 59 621.6 0 59 89 63 621.6 0 63 90 77 621.6 0 77 91 78 621.6 0 78 92 71 621.6 0 71 93 73 621.6 0 73 94 92 621.6 0 92 95 123 621.6 0 123 96 1002 621.6 0 1002 97 321 621.6 0 321 98 837 621.6 0 837 99 100 621.6 0 100 100 87 621.6 0 87 101 81 621.6 0 81 102 91 621.6 0 91 103 88 621.6 0 88 104 54 621.6 0 54 105 58 621.6 0 58 106 69 621.6 0 69 107 75 621.6 0 75 108 200 621.6 0 200 109 102 621.6 0 102 110 85 621.6 0 85 111 69 621.6 0 69 112 72 621.6 0 72 113 81 621.6 0 81 114 74 621.6 0 74 115 91 621.6 0 91 116 75 621.6 0 75 117 88 621.6 0 88 118 72 621.6 0 72 119 71 621.6 0 71 120 80 621.6 0 80 121 86 621.6 0 86 122 54 621.6 0 54 123 82 621.6 0 82 124 77 621.6 0 77 125 66 621.6 0 66 126 67 621.6 0 67 127 106 621.6 0 106 128 116 621.6 0 116 129 82 621.6 0 82 130 157 621.6 0 157 131 160 621.6 0 160 132 315 621.6 0 315 133 107 621.6 0 107 134 216 621.6 0 216 135 95 621.6 0 95 136 78 621.6 0 78 137 115 621.6 0 115 138 83 621.6 0 83 139 97 621.6 0 97 140 80 621.6 0 80 141 65 621.6 0 65 142 63 621.6 0 63 143 97 621.6 0 97 144 70 621.6 0 70 145 67 621.6 0 67 146 73 621.6 0 73 147 88 621.6 0 88 148 237 621.6 0 237 149 147 621.6 0 147 150 739 621.6 0 739 151 60 621.6 0 60 152 62 621.6 0 62 153 68 621.6 0 68 154 67 621.6 0 67 155 66 621.6 0 66 156 81 621.6 0 81 157 63 621.6 0 63 158 57 621.6 0 57 159 48 621.6 0 48 160 58 621.6 0 58 161 80 621.6 0 80 162 70 621.6 0 70 163 58 621.6 0 58 164 70 621.6 0 70 165 71 621.6 0 71 166 64 621.6 0 64 167 79 621.6 0 79 168 48 621.6 0 48 169 70 621.6 0 70 170 92 621.6 0 92 171 69 621.6 0 69 172 74 621.6 0 74 173 75 621.6 0 75 174 66 621.6 0 66 175 79 621.6 0 79 176 82 621.6 0 82 177 56 621.6 0 56 178 70 621.6 0 70 179 69 621.6 0 69 180 62 621.6 0 62 181 85 621.6 0 85 182 50 621.6 0 50 183 80 621.6 0 80 184 95 621.6 0 95 185 111 621.6 0 111 186 141 621.6 0 141 187 1110 621.6 0 1110 188 605 621.6 0 605 189 416 621.6 0 416 190 633 621.6 0 633 191 75 621.6 0 75 192 64 621.6 0 64 193 51 621.6 0 51 194 65 621.6 0 65 195 65 621.6 0 65 196 93 621.6 0 93 197 64 621.6 0 64 198 70 621.6 0 70 199 75 621.6 0 75 200 61 621.6 0 61 201 74 621.6 0 74 202 54 621.6 0 54 203 83 621.6 0 83 204 56 621.6 0 56 205 93 621.6 0 93 206 77 621.6 0 77 207 84 621.6 0 84 208 107 621.6 0 107 209 236 621.6 0 236 210 926 621.6 0 926 211 225 621.6 0 225 212 125 621.6 0 125 213 74 621.6 0 74 214 88 621.6 0 88 215 66 621.6 0 66 216 58 621.6 0 58 217 68 621.6 0 68 218 59 621.6 0 59 219 95 621.6 0 95 220 62 621.6 0 62 221 65 621.6 0 65 222 76 621.6 0 76 223 207 621.6 0 207 224 131 621.6 0 131 225 107 621.6 0 107 226 195 621.6 0 195 227 170 621.6 0 170 228 151 621.6 0 151 229 60 621.6 0 60 230 82 621.6 0 82 231 94 621.6 0 94 232 78 621.6 0 78 233 93 621.6 0 93 234 104 621.6 0 104 235 126 621.6 0 126 236 119 621.6 0 119 237 82 621.6 0 82 238 77 621.6 0 77 239 74 621.6 0 74 240 231 621.6 0 231 241 223 621.6 0 223 242 350 621.6 0 350 243 192 621.6 0 192 244 223 621.6 0 223 245 140 621.6 0 140 246 434 621.6 0 434 247 241 621.6 0 241 248 781 621.6 0 781 249 469 621.6 0 469 250 253 621.6 0 253 251 324 621.6 0 324 252 151 621.6 0 151 253 356 621.6 0 356 254 533 621.6 0 533 255 381 621.6 0 381 256 143 621.6 0 143 257 90 621.6 0 90 258 103 621.6 0 103 259 53 621.6 0 53 260 237 621.6 0 237 261 453 621.6 0 453 262 232 621.6 0 232 263 77 621.6 0 77 264 45 621.6 0 45 265 169 621.6 0 169 266 113 621.6 0 113 267 91 621.6 0 91 268 155 621.6 0 155 269 357 621.6 0 357 270 576 621.6 0 576 271 253 621.6 0 253 272 223 621.6 0 223 273 209 621.6 0 209 274 126 621.6 0 126 275 98 621.6 0 98 276 58 621.6 0 58 277 50 621.6 0 50 278 59 621.6 0 59 279 51 621.6 0 51 280 53 621.6 0 53 281 72 621.6 0 72 282 77 621.6 0 77 283 77 621.6 0 77 284 69 621.6 0 69 285 76 621.6 0 76 286 58 621.6 0 58 287 64 621.6 0 64 288 70 621.6 0 70 289 63 621.6 0 63 290 58 621.6 0 58 291 56 621.6 0 56 292 88 621.6 0 88 293 74 621.6 0 74 294 93 621.6 0 93 295 71 621.6 0 71 296 72 621.6 0 72 297 70 621.6 0 70 298 86 621.6 0 86 299 70 621.6 0 70 300 68 621.6 0 68 301 127 621.6 0 127 Finished in 5.37 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 114,276 Reads with adapters: 43,726 (38.3%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 43,714 (38.3%) Total basepairs processed: 31,094,374 bp Total written (filtered): 12,060,638 bp (38.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43726 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 9 16 355 0.0 3 0 348 7 17 41347 0.0 3 40606 721 17 3 18 68 0.0 3 3 61 2 2 19 42 0.0 3 8 17 15 2 20 1858 0.0 3 1776 31 3 48 21 5 0.0 3 1 4 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 28 2 0.0 3 1 0 0 1 33 1 0.0 3 1 34 2 0.0 3 1 0 0 1 36 1 0.0 3 1 44 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 70 6 0.0 3 0 0 0 6 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 43,714 Reads with adapters: 729 (1.7%) Reads written (passing filters): 43,714 (100.0%) Total basepairs processed: 12,060,638 bp Total written (filtered): 12,058,146 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 729 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.9% C: 4.4% G: 44.2% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 424 683.0 0 424 4 305 170.8 0 305