This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.30 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 40,816 (1.6%) Reads that were too short: 13,442 (0.5%) Reads written (passing filters): 27,374 (1.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,963,600 bp (0.8%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 40816 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 194 621.6 0 194 7 4663 621.6 0 4663 8 3788 621.6 0 3788 9 78 621.6 0 78 10 342 621.6 0 342 11 57 621.6 0 57 12 59 621.6 0 59 13 45 621.6 0 45 14 70 621.6 0 70 15 39 621.6 0 39 16 55 621.6 0 55 17 35 621.6 0 35 18 35 621.6 0 35 19 45 621.6 0 45 20 59 621.6 0 59 21 53 621.6 0 53 22 64 621.6 0 64 23 53 621.6 0 53 24 56 621.6 0 56 25 57 621.6 0 57 26 44 621.6 0 44 27 39 621.6 0 39 28 31 621.6 0 31 29 50 621.6 0 50 30 67 621.6 0 67 31 55 621.6 0 55 32 37 621.6 0 37 33 39 621.6 0 39 34 51 621.6 0 51 35 40 621.6 0 40 36 64 621.6 0 64 37 72 621.6 0 72 38 132 621.6 0 132 39 81 621.6 0 81 40 100 621.6 0 100 41 67 621.6 0 67 42 48 621.6 0 48 43 44 621.6 0 44 44 44 621.6 0 44 45 39 621.6 0 39 46 56 621.6 0 56 47 47 621.6 0 47 48 162 621.6 0 162 49 77 621.6 0 77 50 38 621.6 0 38 51 47 621.6 0 47 52 50 621.6 0 50 53 40 621.6 0 40 54 51 621.6 0 51 55 62 621.6 0 62 56 55 621.6 0 55 57 51 621.6 0 51 58 46 621.6 0 46 59 49 621.6 0 49 60 95 621.6 0 95 61 82 621.6 0 82 62 68 621.6 0 68 63 38 621.6 0 38 64 49 621.6 0 49 65 64 621.6 0 64 66 49 621.6 0 49 67 56 621.6 0 56 68 81 621.6 0 81 69 214 621.6 0 214 70 134 621.6 0 134 71 726 621.6 0 726 72 108 621.6 0 108 73 125 621.6 0 125 74 74 621.6 0 74 75 55 621.6 0 55 76 42 621.6 0 42 77 45 621.6 0 45 78 40 621.6 0 40 79 75 621.6 0 75 80 84 621.6 0 84 81 271 621.6 0 271 82 146 621.6 0 146 83 124 621.6 0 124 84 471 621.6 0 471 85 306 621.6 0 306 86 344 621.6 0 344 87 64 621.6 0 64 88 57 621.6 0 57 89 55 621.6 0 55 90 129 621.6 0 129 91 79 621.6 0 79 92 63 621.6 0 63 93 52 621.6 0 52 94 63 621.6 0 63 95 55 621.6 0 55 96 51 621.6 0 51 97 83 621.6 0 83 98 65 621.6 0 65 99 66 621.6 0 66 100 74 621.6 0 74 101 73 621.6 0 73 102 67 621.6 0 67 103 43 621.6 0 43 104 49 621.6 0 49 105 49 621.6 0 49 106 57 621.6 0 57 107 78 621.6 0 78 108 57 621.6 0 57 109 50 621.6 0 50 110 204 621.6 0 204 111 231 621.6 0 231 112 211 621.6 0 211 113 57 621.6 0 57 114 55 621.6 0 55 115 32 621.6 0 32 116 48 621.6 0 48 117 41 621.6 0 41 118 38 621.6 0 38 119 40 621.6 0 40 120 48 621.6 0 48 121 53 621.6 0 53 122 57 621.6 0 57 123 54 621.6 0 54 124 54 621.6 0 54 125 58 621.6 0 58 126 44 621.6 0 44 127 38 621.6 0 38 128 49 621.6 0 49 129 50 621.6 0 50 130 155 621.6 0 155 131 71 621.6 0 71 132 83 621.6 0 83 133 66 621.6 0 66 134 48 621.6 0 48 135 42 621.6 0 42 136 45 621.6 0 45 137 55 621.6 0 55 138 73 621.6 0 73 139 62 621.6 0 62 140 58 621.6 0 58 141 69 621.6 0 69 142 72 621.6 0 72 143 81 621.6 0 81 144 66 621.6 0 66 145 113 621.6 0 113 146 132 621.6 0 132 147 86 621.6 0 86 148 129 621.6 0 129 149 112 621.6 0 112 150 125 621.6 0 125 151 130 621.6 0 130 152 89 621.6 0 89 153 70 621.6 0 70 154 67 621.6 0 67 155 61 621.6 0 61 156 295 621.6 0 295 157 210 621.6 0 210 158 120 621.6 0 120 159 89 621.6 0 89 160 52 621.6 0 52 161 88 621.6 0 88 162 47 621.6 0 47 163 45 621.6 0 45 164 48 621.6 0 48 165 46 621.6 0 46 166 61 621.6 0 61 167 56 621.6 0 56 168 126 621.6 0 126 169 128 621.6 0 128 170 162 621.6 0 162 171 82 621.6 0 82 172 139 621.6 0 139 173 57 621.6 0 57 174 76 621.6 0 76 175 81 621.6 0 81 176 282 621.6 0 282 177 155 621.6 0 155 178 273 621.6 0 273 179 46 621.6 0 46 180 59 621.6 0 59 181 99 621.6 0 99 182 69 621.6 0 69 183 54 621.6 0 54 184 101 621.6 0 101 185 52 621.6 0 52 186 114 621.6 0 114 187 162 621.6 0 162 188 154 621.6 0 154 189 153 621.6 0 153 190 210 621.6 0 210 191 95 621.6 0 95 192 60 621.6 0 60 193 65 621.6 0 65 194 92 621.6 0 92 195 116 621.6 0 116 196 172 621.6 0 172 197 562 621.6 0 562 198 587 621.6 0 587 199 591 621.6 0 591 200 76 621.6 0 76 201 78 621.6 0 78 202 235 621.6 0 235 203 274 621.6 0 274 204 492 621.6 0 492 205 269 621.6 0 269 206 152 621.6 0 152 207 56 621.6 0 56 208 59 621.6 0 59 209 93 621.6 0 93 210 124 621.6 0 124 211 104 621.6 0 104 212 116 621.6 0 116 213 227 621.6 0 227 214 204 621.6 0 204 215 175 621.6 0 175 216 86 621.6 0 86 217 71 621.6 0 71 218 86 621.6 0 86 219 92 621.6 0 92 220 68 621.6 0 68 221 67 621.6 0 67 222 163 621.6 0 163 223 557 621.6 0 557 224 622 621.6 0 622 225 549 621.6 0 549 226 74 621.6 0 74 227 55 621.6 0 55 228 71 621.6 0 71 229 55 621.6 0 55 230 51 621.6 0 51 231 58 621.6 0 58 232 46 621.6 0 46 233 70 621.6 0 70 234 85 621.6 0 85 235 92 621.6 0 92 236 64 621.6 0 64 237 53 621.6 0 53 238 64 621.6 0 64 239 103 621.6 0 103 240 89 621.6 0 89 241 168 621.6 0 168 242 142 621.6 0 142 243 135 621.6 0 135 244 142 621.6 0 142 245 114 621.6 0 114 246 114 621.6 0 114 247 83 621.6 0 83 248 89 621.6 0 89 249 86 621.6 0 86 250 64 621.6 0 64 251 82 621.6 0 82 252 59 621.6 0 59 253 62 621.6 0 62 254 64 621.6 0 64 255 89 621.6 0 89 256 83 621.6 0 83 257 179 621.6 0 179 258 340 621.6 0 340 259 157 621.6 0 157 260 222 621.6 0 222 261 136 621.6 0 136 262 64 621.6 0 64 263 71 621.6 0 71 264 50 621.6 0 50 265 62 621.6 0 62 266 51 621.6 0 51 267 54 621.6 0 54 268 57 621.6 0 57 269 79 621.6 0 79 270 102 621.6 0 102 271 241 621.6 0 241 272 191 621.6 0 191 273 166 621.6 0 166 274 59 621.6 0 59 275 68 621.6 0 68 276 76 621.6 0 76 277 135 621.6 0 135 278 100 621.6 0 100 279 134 621.6 0 134 280 85 621.6 0 85 281 95 621.6 0 95 282 87 621.6 0 87 283 100 621.6 0 100 284 160 621.6 0 160 285 126 621.6 0 126 286 107 621.6 0 107 287 105 621.6 0 105 288 103 621.6 0 103 289 82 621.6 0 82 290 56 621.6 0 56 291 83 621.6 0 83 292 92 621.6 0 92 293 88 621.6 0 88 294 112 621.6 0 112 295 108 621.6 0 108 296 125 621.6 0 125 297 210 621.6 0 210 298 365 621.6 0 365 299 424 621.6 0 424 300 232 621.6 0 232 301 156 621.6 0 156 Finished in 1.27 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 27,374 Reads with adapters: 3,961 (14.5%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 3,953 (14.4%) Total basepairs processed: 5,963,600 bp Total written (filtered): 1,078,127 bp (18.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3961 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 80 0.0 3 0 68 8 4 20 3828 0.0 4 3521 226 65 15 1 21 12 0.0 4 1 10 1 22 1 0.0 4 0 0 0 0 1 23 14 0.0 4 1 0 0 9 4 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 1 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 58 1 0.0 4 0 0 0 0 1 67 1 0.0 4 1 69 1 0.0 4 0 1 75 1 0.0 4 0 0 1 77 1 0.0 4 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 139 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 3,953 Reads with adapters: 811 (20.5%) Reads written (passing filters): 3,953 (100.0%) Total basepairs processed: 1,078,127 bp Total written (filtered): 1,068,435 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 811 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 8.3% G: 85.9% T: 3.3% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 61.8 0 6 4 1 15.4 0 1 7 1 0.2 0 1 9 2 0.0 0 0 2 10 128 0.0 1 5 123 11 658 0.0 1 31 627 12 5 0.0 1 0 5 14 4 0.0 1 0 4 19 1 0.0 1 1 150 1 0.0 1 0 1 151 1 0.0 1 0 1 209 2 0.0 1 0 2 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.