This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.13 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 31,622 (1.2%) Reads that were too short: 7,714 (0.3%) Reads written (passing filters): 23,908 (0.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,516,776 bp (0.7%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 31622 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 211 621.6 0 211 7 4114 621.6 0 4114 8 4860 621.6 0 4860 9 59 621.6 0 59 10 64 621.6 0 64 11 82 621.6 0 82 12 53 621.6 0 53 13 40 621.6 0 40 14 95 621.6 0 95 15 55 621.6 0 55 16 59 621.6 0 59 17 51 621.6 0 51 18 48 621.6 0 48 19 59 621.6 0 59 20 44 621.6 0 44 21 53 621.6 0 53 22 47 621.6 0 47 23 61 621.6 0 61 24 56 621.6 0 56 25 57 621.6 0 57 26 41 621.6 0 41 27 52 621.6 0 52 28 38 621.6 0 38 29 52 621.6 0 52 30 52 621.6 0 52 31 44 621.6 0 44 32 30 621.6 0 30 33 27 621.6 0 27 34 49 621.6 0 49 35 24 621.6 0 24 36 59 621.6 0 59 37 53 621.6 0 53 38 57 621.6 0 57 39 49 621.6 0 49 40 44 621.6 0 44 41 51 621.6 0 51 42 63 621.6 0 63 43 58 621.6 0 58 44 47 621.6 0 47 45 49 621.6 0 49 46 56 621.6 0 56 47 26 621.6 0 26 48 54 621.6 0 54 49 57 621.6 0 57 50 50 621.6 0 50 51 59 621.6 0 59 52 56 621.6 0 56 53 49 621.6 0 49 54 54 621.6 0 54 55 70 621.6 0 70 56 73 621.6 0 73 57 56 621.6 0 56 58 55 621.6 0 55 59 37 621.6 0 37 60 61 621.6 0 61 61 50 621.6 0 50 62 47 621.6 0 47 63 43 621.6 0 43 64 56 621.6 0 56 65 58 621.6 0 58 66 51 621.6 0 51 67 48 621.6 0 48 68 60 621.6 0 60 69 249 621.6 0 249 70 115 621.6 0 115 71 1115 621.6 0 1115 72 133 621.6 0 133 73 114 621.6 0 114 74 63 621.6 0 63 75 80 621.6 0 80 76 58 621.6 0 58 77 43 621.6 0 43 78 52 621.6 0 52 79 43 621.6 0 43 80 67 621.6 0 67 81 241 621.6 0 241 82 95 621.6 0 95 83 58 621.6 0 58 84 303 621.6 0 303 85 223 621.6 0 223 86 322 621.6 0 322 87 36 621.6 0 36 88 40 621.6 0 40 89 47 621.6 0 47 90 42 621.6 0 42 91 41 621.6 0 41 92 39 621.6 0 39 93 46 621.6 0 46 94 63 621.6 0 63 95 66 621.6 0 66 96 57 621.6 0 57 97 58 621.6 0 58 98 37 621.6 0 37 99 64 621.6 0 64 100 62 621.6 0 62 101 50 621.6 0 50 102 57 621.6 0 57 103 33 621.6 0 33 104 37 621.6 0 37 105 53 621.6 0 53 106 59 621.6 0 59 107 66 621.6 0 66 108 56 621.6 0 56 109 57 621.6 0 57 110 174 621.6 0 174 111 230 621.6 0 230 112 204 621.6 0 204 113 48 621.6 0 48 114 36 621.6 0 36 115 30 621.6 0 30 116 49 621.6 0 49 117 42 621.6 0 42 118 38 621.6 0 38 119 35 621.6 0 35 120 64 621.6 0 64 121 48 621.6 0 48 122 56 621.6 0 56 123 41 621.6 0 41 124 55 621.6 0 55 125 57 621.6 0 57 126 42 621.6 0 42 127 35 621.6 0 35 128 51 621.6 0 51 129 53 621.6 0 53 130 151 621.6 0 151 131 74 621.6 0 74 132 50 621.6 0 50 133 59 621.6 0 59 134 38 621.6 0 38 135 54 621.6 0 54 136 55 621.6 0 55 137 55 621.6 0 55 138 50 621.6 0 50 139 55 621.6 0 55 140 50 621.6 0 50 141 52 621.6 0 52 142 52 621.6 0 52 143 72 621.6 0 72 144 42 621.6 0 42 145 62 621.6 0 62 146 90 621.6 0 90 147 80 621.6 0 80 148 95 621.6 0 95 149 101 621.6 0 101 150 91 621.6 0 91 151 60 621.6 0 60 152 62 621.6 0 62 153 50 621.6 0 50 154 64 621.6 0 64 155 55 621.6 0 55 156 134 621.6 0 134 157 105 621.6 0 105 158 88 621.6 0 88 159 60 621.6 0 60 160 42 621.6 0 42 161 88 621.6 0 88 162 36 621.6 0 36 163 51 621.6 0 51 164 46 621.6 0 46 165 45 621.6 0 45 166 59 621.6 0 59 167 45 621.6 0 45 168 102 621.6 0 102 169 116 621.6 0 116 170 130 621.6 0 130 171 88 621.6 0 88 172 119 621.6 0 119 173 47 621.6 0 47 174 57 621.6 0 57 175 54 621.6 0 54 176 123 621.6 0 123 177 70 621.6 0 70 178 101 621.6 0 101 179 45 621.6 0 45 180 56 621.6 0 56 181 48 621.6 0 48 182 57 621.6 0 57 183 50 621.6 0 50 184 63 621.6 0 63 185 44 621.6 0 44 186 67 621.6 0 67 187 70 621.6 0 70 188 101 621.6 0 101 189 74 621.6 0 74 190 98 621.6 0 98 191 69 621.6 0 69 192 56 621.6 0 56 193 59 621.6 0 59 194 72 621.6 0 72 195 58 621.6 0 58 196 104 621.6 0 104 197 276 621.6 0 276 198 286 621.6 0 286 199 287 621.6 0 287 200 43 621.6 0 43 201 39 621.6 0 39 202 104 621.6 0 104 203 138 621.6 0 138 204 183 621.6 0 183 205 113 621.6 0 113 206 69 621.6 0 69 207 55 621.6 0 55 208 41 621.6 0 41 209 72 621.6 0 72 210 53 621.6 0 53 211 58 621.6 0 58 212 56 621.6 0 56 213 58 621.6 0 58 214 69 621.6 0 69 215 70 621.6 0 70 216 40 621.6 0 40 217 62 621.6 0 62 218 50 621.6 0 50 219 59 621.6 0 59 220 52 621.6 0 52 221 46 621.6 0 46 222 78 621.6 0 78 223 257 621.6 0 257 224 292 621.6 0 292 225 282 621.6 0 282 226 60 621.6 0 60 227 52 621.6 0 52 228 77 621.6 0 77 229 45 621.6 0 45 230 48 621.6 0 48 231 50 621.6 0 50 232 51 621.6 0 51 233 56 621.6 0 56 234 61 621.6 0 61 235 56 621.6 0 56 236 68 621.6 0 68 237 47 621.6 0 47 238 45 621.6 0 45 239 79 621.6 0 79 240 77 621.6 0 77 241 59 621.6 0 59 242 61 621.6 0 61 243 76 621.6 0 76 244 66 621.6 0 66 245 64 621.6 0 64 246 64 621.6 0 64 247 62 621.6 0 62 248 77 621.6 0 77 249 63 621.6 0 63 250 83 621.6 0 83 251 56 621.6 0 56 252 48 621.6 0 48 253 52 621.6 0 52 254 73 621.6 0 73 255 65 621.6 0 65 256 67 621.6 0 67 257 76 621.6 0 76 258 181 621.6 0 181 259 90 621.6 0 90 260 163 621.6 0 163 261 98 621.6 0 98 262 59 621.6 0 59 263 64 621.6 0 64 264 41 621.6 0 41 265 46 621.6 0 46 266 36 621.6 0 36 267 46 621.6 0 46 268 54 621.6 0 54 269 50 621.6 0 50 270 61 621.6 0 61 271 102 621.6 0 102 272 105 621.6 0 105 273 84 621.6 0 84 274 56 621.6 0 56 275 52 621.6 0 52 276 56 621.6 0 56 277 76 621.6 0 76 278 47 621.6 0 47 279 92 621.6 0 92 280 57 621.6 0 57 281 61 621.6 0 61 282 61 621.6 0 61 283 74 621.6 0 74 284 95 621.6 0 95 285 59 621.6 0 59 286 67 621.6 0 67 287 66 621.6 0 66 288 76 621.6 0 76 289 48 621.6 0 48 290 38 621.6 0 38 291 61 621.6 0 61 292 54 621.6 0 54 293 50 621.6 0 50 294 73 621.6 0 73 295 61 621.6 0 61 296 49 621.6 0 49 297 99 621.6 0 99 298 170 621.6 0 170 299 198 621.6 0 198 300 123 621.6 0 123 301 74 621.6 0 74 Finished in 1.03 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 23,908 Reads with adapters: 3,944 (16.5%) Reads that were too short: 18 (0.1%) Reads written (passing filters): 3,926 (16.4%) Total basepairs processed: 5,516,776 bp Total written (filtered): 1,086,653 bp (19.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3944 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 28 0.0 3 0 28 17 3873 0.0 3 3773 98 2 18 6 0.0 3 2 4 19 2 0.0 3 1 0 1 20 6 0.0 3 0 0 0 6 33 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 65 2 0.0 3 2 66 1 0.0 3 1 68 1 0.0 3 0 1 69 1 0.0 3 1 73 1 0.0 3 0 1 74 1 0.0 3 0 1 80 1 0.0 3 0 0 0 1 89 1 0.0 3 1 107 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 1 207 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 3,926 Reads with adapters: 9 (0.2%) Reads written (passing filters): 3,926 (100.0%) Total basepairs processed: 1,086,653 bp Total written (filtered): 1,086,626 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.1% C: 55.6% G: 22.2% T: 11.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 61.3 0 9