This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.04 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 313,376 (12.3%) Reads that were too short: 58,065 (2.3%) Reads written (passing filters): 255,311 (10.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 50,268,255 bp (6.6%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 313376 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4751 621.6 0 4751 7 31494 621.6 0 31494 8 628 621.6 0 628 9 37035 621.6 0 37035 10 69 621.6 0 69 11 59 621.6 0 59 12 72 621.6 0 72 13 55 621.6 0 55 14 57 621.6 0 57 15 94 621.6 0 94 16 113 621.6 0 113 17 88 621.6 0 88 18 51 621.6 0 51 19 69 621.6 0 69 20 45 621.6 0 45 21 63 621.6 0 63 22 51 621.6 0 51 23 57 621.6 0 57 24 76 621.6 0 76 25 69 621.6 0 69 26 53 621.6 0 53 27 59 621.6 0 59 28 65 621.6 0 65 29 55 621.6 0 55 30 57 621.6 0 57 31 94 621.6 0 94 32 60 621.6 0 60 33 65 621.6 0 65 34 79 621.6 0 79 35 60 621.6 0 60 36 70 621.6 0 70 37 86 621.6 0 86 38 74 621.6 0 74 39 81 621.6 0 81 40 62 621.6 0 62 41 62 621.6 0 62 42 65 621.6 0 65 43 74 621.6 0 74 44 68 621.6 0 68 45 49 621.6 0 49 46 53 621.6 0 53 47 85 621.6 0 85 48 74 621.6 0 74 49 50 621.6 0 50 50 68 621.6 0 68 51 51 621.6 0 51 52 49 621.6 0 49 53 43 621.6 0 43 54 41 621.6 0 41 55 53 621.6 0 53 56 113 621.6 0 113 57 100 621.6 0 100 58 125 621.6 0 125 59 70 621.6 0 70 60 55 621.6 0 55 61 62 621.6 0 62 62 78 621.6 0 78 63 57 621.6 0 57 64 54 621.6 0 54 65 52 621.6 0 52 66 63 621.6 0 63 67 73 621.6 0 73 68 76 621.6 0 76 69 61 621.6 0 61 70 76 621.6 0 76 71 195 621.6 0 195 72 117 621.6 0 117 73 246 621.6 0 246 74 62 621.6 0 62 75 54 621.6 0 54 76 69 621.6 0 69 77 56 621.6 0 56 78 75 621.6 0 75 79 74 621.6 0 74 80 52 621.6 0 52 81 49 621.6 0 49 82 90 621.6 0 90 83 65 621.6 0 65 84 52 621.6 0 52 85 74 621.6 0 74 86 78 621.6 0 78 87 126 621.6 0 126 88 87 621.6 0 87 89 154 621.6 0 154 90 103 621.6 0 103 91 101 621.6 0 101 92 63 621.6 0 63 93 62 621.6 0 62 94 54 621.6 0 54 95 50 621.6 0 50 96 66 621.6 0 66 97 70 621.6 0 70 98 79 621.6 0 79 99 54 621.6 0 54 100 81 621.6 0 81 101 68 621.6 0 68 102 116 621.6 0 116 103 389 621.6 0 389 104 478 621.6 0 478 105 328 621.6 0 328 106 260 621.6 0 260 107 156 621.6 0 156 108 176 621.6 0 176 109 124 621.6 0 124 110 72 621.6 0 72 111 112 621.6 0 112 112 121 621.6 0 121 113 114 621.6 0 114 114 84 621.6 0 84 115 96 621.6 0 96 116 269 621.6 0 269 117 88 621.6 0 88 118 117 621.6 0 117 119 556 621.6 0 556 120 78 621.6 0 78 121 116 621.6 0 116 122 74 621.6 0 74 123 106 621.6 0 106 124 77 621.6 0 77 125 72 621.6 0 72 126 170 621.6 0 170 127 263 621.6 0 263 128 313 621.6 0 313 129 927 621.6 0 927 130 192 621.6 0 192 131 337 621.6 0 337 132 291 621.6 0 291 133 254 621.6 0 254 134 785 621.6 0 785 135 483 621.6 0 483 136 467 621.6 0 467 137 205 621.6 0 205 138 197 621.6 0 197 139 483 621.6 0 483 140 791 621.6 0 791 141 756 621.6 0 756 142 1142 621.6 0 1142 143 893 621.6 0 893 144 1982 621.6 0 1982 145 6030 621.6 0 6030 146 40685 621.6 0 40685 147 57124 621.6 0 57124 148 41355 621.6 0 41355 149 494 621.6 0 494 150 2624 621.6 0 2624 151 839 621.6 0 839 152 4084 621.6 0 4084 153 79 621.6 0 79 154 89 621.6 0 89 155 131 621.6 0 131 156 535 621.6 0 535 157 393 621.6 0 393 158 626 621.6 0 626 159 104 621.6 0 104 160 139 621.6 0 139 161 183 621.6 0 183 162 158 621.6 0 158 163 111 621.6 0 111 164 74 621.6 0 74 165 74 621.6 0 74 166 103 621.6 0 103 167 71 621.6 0 71 168 117 621.6 0 117 169 64 621.6 0 64 170 66 621.6 0 66 171 67 621.6 0 67 172 55 621.6 0 55 173 56 621.6 0 56 174 65 621.6 0 65 175 134 621.6 0 134 176 145 621.6 0 145 177 165 621.6 0 165 178 77 621.6 0 77 179 77 621.6 0 77 180 132 621.6 0 132 181 226 621.6 0 226 182 172 621.6 0 172 183 168 621.6 0 168 184 138 621.6 0 138 185 179 621.6 0 179 186 248 621.6 0 248 187 105 621.6 0 105 188 135 621.6 0 135 189 73 621.6 0 73 190 57 621.6 0 57 191 56 621.6 0 56 192 49 621.6 0 49 193 56 621.6 0 56 194 60 621.6 0 60 195 59 621.6 0 59 196 78 621.6 0 78 197 58 621.6 0 58 198 62 621.6 0 62 199 78 621.6 0 78 200 104 621.6 0 104 201 73 621.6 0 73 202 111 621.6 0 111 203 158 621.6 0 158 204 184 621.6 0 184 205 151 621.6 0 151 206 148 621.6 0 148 207 128 621.6 0 128 208 238 621.6 0 238 209 169 621.6 0 169 210 160 621.6 0 160 211 137 621.6 0 137 212 430 621.6 0 430 213 182 621.6 0 182 214 151 621.6 0 151 215 103 621.6 0 103 216 82 621.6 0 82 217 64 621.6 0 64 218 80 621.6 0 80 219 149 621.6 0 149 220 304 621.6 0 304 221 247 621.6 0 247 222 320 621.6 0 320 223 162 621.6 0 162 224 172 621.6 0 172 225 109 621.6 0 109 226 192 621.6 0 192 227 128 621.6 0 128 228 86 621.6 0 86 229 148 621.6 0 148 230 127 621.6 0 127 231 121 621.6 0 121 232 138 621.6 0 138 233 112 621.6 0 112 234 144 621.6 0 144 235 190 621.6 0 190 236 136 621.6 0 136 237 146 621.6 0 146 238 150 621.6 0 150 239 184 621.6 0 184 240 128 621.6 0 128 241 144 621.6 0 144 242 118 621.6 0 118 243 216 621.6 0 216 244 249 621.6 0 249 245 187 621.6 0 187 246 154 621.6 0 154 247 101 621.6 0 101 248 129 621.6 0 129 249 164 621.6 0 164 250 195 621.6 0 195 251 230 621.6 0 230 252 283 621.6 0 283 253 373 621.6 0 373 254 641 621.6 0 641 255 1941 621.6 0 1941 256 10722 621.6 0 10722 257 12118 621.6 0 12118 258 13116 621.6 0 13116 259 2376 621.6 0 2376 260 172 621.6 0 172 261 180 621.6 0 180 262 467 621.6 0 467 263 509 621.6 0 509 264 657 621.6 0 657 265 130 621.6 0 130 266 238 621.6 0 238 267 249 621.6 0 249 268 327 621.6 0 327 269 214 621.6 0 214 270 86 621.6 0 86 271 96 621.6 0 96 272 85 621.6 0 85 273 96 621.6 0 96 274 137 621.6 0 137 275 135 621.6 0 135 276 100 621.6 0 100 277 82 621.6 0 82 278 80 621.6 0 80 279 216 621.6 0 216 280 455 621.6 0 455 281 476 621.6 0 476 282 481 621.6 0 481 283 209 621.6 0 209 284 284 621.6 0 284 285 300 621.6 0 300 286 160 621.6 0 160 287 123 621.6 0 123 288 114 621.6 0 114 289 156 621.6 0 156 290 93 621.6 0 93 291 65 621.6 0 65 292 68 621.6 0 68 293 104 621.6 0 104 294 125 621.6 0 125 295 142 621.6 0 142 296 91 621.6 0 91 297 107 621.6 0 107 298 86 621.6 0 86 299 88 621.6 0 88 300 123 621.6 0 123 301 233 621.6 0 233 Finished in 10.18 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 255,311 Reads with adapters: 35,745 (14.0%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 35,694 (14.0%) Total basepairs processed: 50,268,255 bp Total written (filtered): 9,761,434 bp (19.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35745 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 21 0.0 3 0 0 16 3 2 19 421 0.0 3 0 330 44 31 16 20 34865 0.0 4 32254 2009 423 126 53 21 90 0.0 4 15 70 1 2 2 22 11 0.0 4 0 2 7 2 23 90 0.0 4 20 1 1 49 19 24 1 0.0 4 0 0 0 0 1 28 5 0.0 4 0 0 0 0 5 33 1 0.0 4 0 0 0 0 1 34 20 0.0 4 0 0 0 0 20 37 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 75 1 0.0 4 0 1 76 1 0.0 4 0 0 0 0 1 77 9 0.0 4 0 0 0 0 9 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 140 0.0 4 0 0 0 0 140 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 135 9 0.0 4 0 0 0 0 9 139 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 244 1 0.0 4 0 0 0 1 250 1 0.0 4 0 1 251 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 1 265 1 0.0 4 0 0 1 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 35,694 Reads with adapters: 1,826 (5.1%) Reads written (passing filters): 35,694 (100.0%) Total basepairs processed: 9,761,434 bp Total written (filtered): 9,678,600 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1826 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.8% C: 15.4% G: 61.1% T: 11.2% none/other: 3.5% Overview of removed sequences length count expect max.err error counts 3 183 557.7 0 183 4 43 139.4 0 43 9 12 0.1 0 2 10 10 53 0.0 1 2 51 11 931 0.0 1 38 893 12 324 0.0 1 12 312 13 23 0.0 1 0 23 14 1 0.0 1 0 1 15 2 0.0 1 0 2 152 7 0.0 1 6 1 153 1 0.0 1 1 171 1 0.0 1 1 210 15 0.0 1 10 5 211 2 0.0 1 2 259 1 0.0 1 1 269 1 0.0 1 1 270 1 0.0 1 1 271 117 0.0 1 113 4 272 15 0.0 1 14 1 273 29 0.0 1 27 2 274 59 0.0 1 52 7 275 5 0.0 1 5