This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.58 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 77,741 (3.1%) Reads that were too short: 19,872 (0.8%) Reads written (passing filters): 57,869 (2.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 10,547,058 bp (1.4%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 77741 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2006 621.6 0 2006 7 5794 621.6 0 5794 8 303 621.6 0 303 9 4687 621.6 0 4687 10 57 621.6 0 57 11 65 621.6 0 65 12 29 621.6 0 29 13 40 621.6 0 40 14 29 621.6 0 29 15 70 621.6 0 70 16 31 621.6 0 31 17 37 621.6 0 37 18 27 621.6 0 27 19 25 621.6 0 25 20 35 621.6 0 35 21 33 621.6 0 33 22 55 621.6 0 55 23 40 621.6 0 40 24 35 621.6 0 35 25 26 621.6 0 26 26 39 621.6 0 39 27 33 621.6 0 33 28 37 621.6 0 37 29 35 621.6 0 35 30 56 621.6 0 56 31 25 621.6 0 25 32 38 621.6 0 38 33 51 621.6 0 51 34 27 621.6 0 27 35 58 621.6 0 58 36 40 621.6 0 40 37 36 621.6 0 36 38 65 621.6 0 65 39 39 621.6 0 39 40 138 621.6 0 138 41 73 621.6 0 73 42 101 621.6 0 101 43 32 621.6 0 32 44 29 621.6 0 29 45 54 621.6 0 54 46 30 621.6 0 30 47 40 621.6 0 40 48 31 621.6 0 31 49 25 621.6 0 25 50 31 621.6 0 31 51 30 621.6 0 30 52 35 621.6 0 35 53 36 621.6 0 36 54 34 621.6 0 34 55 159 621.6 0 159 56 92 621.6 0 92 57 141 621.6 0 141 58 51 621.6 0 51 59 57 621.6 0 57 60 98 621.6 0 98 61 47 621.6 0 47 62 64 621.6 0 64 63 43 621.6 0 43 64 52 621.6 0 52 65 38 621.6 0 38 66 81 621.6 0 81 67 34 621.6 0 34 68 86 621.6 0 86 69 39 621.6 0 39 70 33 621.6 0 33 71 84 621.6 0 84 72 35 621.6 0 35 73 45 621.6 0 45 74 32 621.6 0 32 75 79 621.6 0 79 76 37 621.6 0 37 77 30 621.6 0 30 78 28 621.6 0 28 79 41 621.6 0 41 80 23 621.6 0 23 81 34 621.6 0 34 82 28 621.6 0 28 83 50 621.6 0 50 84 77 621.6 0 77 85 48 621.6 0 48 86 34 621.6 0 34 87 29 621.6 0 29 88 43 621.6 0 43 89 60 621.6 0 60 90 63 621.6 0 63 91 65 621.6 0 65 92 48 621.6 0 48 93 57 621.6 0 57 94 46 621.6 0 46 95 51 621.6 0 51 96 38 621.6 0 38 97 40 621.6 0 40 98 35 621.6 0 35 99 22 621.6 0 22 100 24 621.6 0 24 101 28 621.6 0 28 102 57 621.6 0 57 103 100 621.6 0 100 104 96 621.6 0 96 105 392 621.6 0 392 106 284 621.6 0 284 107 417 621.6 0 417 108 28 621.6 0 28 109 45 621.6 0 45 110 47 621.6 0 47 111 60 621.6 0 60 112 64 621.6 0 64 113 84 621.6 0 84 114 91 621.6 0 91 115 91 621.6 0 91 116 47 621.6 0 47 117 76 621.6 0 76 118 53 621.6 0 53 119 37 621.6 0 37 120 58 621.6 0 58 121 49 621.6 0 49 122 51 621.6 0 51 123 57 621.6 0 57 124 52 621.6 0 52 125 80 621.6 0 80 126 117 621.6 0 117 127 282 621.6 0 282 128 892 621.6 0 892 129 390 621.6 0 390 130 611 621.6 0 611 131 121 621.6 0 121 132 138 621.6 0 138 133 114 621.6 0 114 134 181 621.6 0 181 135 98 621.6 0 98 136 103 621.6 0 103 137 181 621.6 0 181 138 83 621.6 0 83 139 88 621.6 0 88 140 94 621.6 0 94 141 109 621.6 0 109 142 211 621.6 0 211 143 127 621.6 0 127 144 212 621.6 0 212 145 232 621.6 0 232 146 291 621.6 0 291 147 4333 621.6 0 4333 148 3488 621.6 0 3488 149 8223 621.6 0 8223 150 131 621.6 0 131 151 71 621.6 0 71 152 239 621.6 0 239 153 182 621.6 0 182 154 455 621.6 0 455 155 116 621.6 0 116 156 119 621.6 0 119 157 96 621.6 0 96 158 73 621.6 0 73 159 82 621.6 0 82 160 61 621.6 0 61 161 64 621.6 0 64 162 65 621.6 0 65 163 55 621.6 0 55 164 61 621.6 0 61 165 43 621.6 0 43 166 42 621.6 0 42 167 41 621.6 0 41 168 93 621.6 0 93 169 147 621.6 0 147 170 198 621.6 0 198 171 107 621.6 0 107 172 394 621.6 0 394 173 289 621.6 0 289 174 267 621.6 0 267 175 66 621.6 0 66 176 59 621.6 0 59 177 70 621.6 0 70 178 101 621.6 0 101 179 72 621.6 0 72 180 63 621.6 0 63 181 45 621.6 0 45 182 88 621.6 0 88 183 151 621.6 0 151 184 147 621.6 0 147 185 113 621.6 0 113 186 175 621.6 0 175 187 415 621.6 0 415 188 2907 621.6 0 2907 189 2250 621.6 0 2250 190 3375 621.6 0 3375 191 115 621.6 0 115 192 52 621.6 0 52 193 94 621.6 0 94 194 42 621.6 0 42 195 75 621.6 0 75 196 150 621.6 0 150 197 133 621.6 0 133 198 184 621.6 0 184 199 257 621.6 0 257 200 486 621.6 0 486 201 2367 621.6 0 2367 202 1530 621.6 0 1530 203 2551 621.6 0 2551 204 296 621.6 0 296 205 79 621.6 0 79 206 89 621.6 0 89 207 44 621.6 0 44 208 80 621.6 0 80 209 51 621.6 0 51 210 65 621.6 0 65 211 34 621.6 0 34 212 40 621.6 0 40 213 65 621.6 0 65 214 70 621.6 0 70 215 80 621.6 0 80 216 52 621.6 0 52 217 34 621.6 0 34 218 28 621.6 0 28 219 35 621.6 0 35 220 29 621.6 0 29 221 55 621.6 0 55 222 56 621.6 0 56 223 54 621.6 0 54 224 74 621.6 0 74 225 98 621.6 0 98 226 149 621.6 0 149 227 137 621.6 0 137 228 248 621.6 0 248 229 945 621.6 0 945 230 217 621.6 0 217 231 115 621.6 0 115 232 75 621.6 0 75 233 43 621.6 0 43 234 58 621.6 0 58 235 48 621.6 0 48 236 66 621.6 0 66 237 51 621.6 0 51 238 45 621.6 0 45 239 51 621.6 0 51 240 51 621.6 0 51 241 75 621.6 0 75 242 56 621.6 0 56 243 70 621.6 0 70 244 58 621.6 0 58 245 88 621.6 0 88 246 65 621.6 0 65 247 82 621.6 0 82 248 92 621.6 0 92 249 128 621.6 0 128 250 158 621.6 0 158 251 119 621.6 0 119 252 163 621.6 0 163 253 89 621.6 0 89 254 59 621.6 0 59 255 54 621.6 0 54 256 29 621.6 0 29 257 58 621.6 0 58 258 139 621.6 0 139 259 96 621.6 0 96 260 332 621.6 0 332 261 248 621.6 0 248 262 177 621.6 0 177 263 93 621.6 0 93 264 70 621.6 0 70 265 84 621.6 0 84 266 83 621.6 0 83 267 87 621.6 0 87 268 86 621.6 0 86 269 655 621.6 0 655 270 257 621.6 0 257 271 383 621.6 0 383 272 147 621.6 0 147 273 90 621.6 0 90 274 107 621.6 0 107 275 123 621.6 0 123 276 164 621.6 0 164 277 142 621.6 0 142 278 131 621.6 0 131 279 167 621.6 0 167 280 362 621.6 0 362 281 251 621.6 0 251 282 644 621.6 0 644 283 249 621.6 0 249 284 230 621.6 0 230 285 78 621.6 0 78 286 82 621.6 0 82 287 135 621.6 0 135 288 79 621.6 0 79 289 123 621.6 0 123 290 126 621.6 0 126 291 491 621.6 0 491 292 306 621.6 0 306 293 689 621.6 0 689 294 714 621.6 0 714 295 623 621.6 0 623 296 759 621.6 0 759 297 248 621.6 0 248 298 84 621.6 0 84 299 145 621.6 0 145 300 136 621.6 0 136 301 126 621.6 0 126 Finished in 2.37 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 57,869 Reads with adapters: 6,284 (10.9%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 6,260 (10.8%) Total basepairs processed: 10,547,058 bp Total written (filtered): 1,701,627 bp (16.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 4 3 19 74 0.0 3 0 63 6 3 2 20 4946 0.0 4 4664 217 49 13 3 21 32 0.0 4 3 23 4 1 1 22 1167 0.0 4 1078 66 18 3 2 23 14 0.0 4 2 2 0 7 3 25 3 0.0 4 0 0 0 3 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 1 1 74 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 129 7 0.0 4 0 0 0 0 7 140 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 6,260 Reads with adapters: 1,568 (25.0%) Reads written (passing filters): 6,260 (100.0%) Total basepairs processed: 1,701,627 bp Total written (filtered): 1,677,299 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1568 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 7.8% G: 83.2% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 97.8 0 2 7 1 0.4 0 1 9 104 0.0 0 10 94 10 1223 0.0 1 83 1140 11 145 0.0 1 7 138 12 2 0.0 1 0 2 13 5 0.0 1 0 5 110 81 0.0 1 66 15 111 5 0.0 1 4 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.