This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.37 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 58,539 (2.3%) Reads that were too short: 10,554 (0.4%) Reads written (passing filters): 47,985 (1.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 9,119,936 bp (1.2%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 58539 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1929 621.6 0 1929 7 6195 621.6 0 6195 8 246 621.6 0 246 9 5423 621.6 0 5423 10 26 621.6 0 26 11 25 621.6 0 25 12 21 621.6 0 21 13 18 621.6 0 18 14 17 621.6 0 17 15 26 621.6 0 26 16 12 621.6 0 12 17 20 621.6 0 20 18 13 621.6 0 13 19 21 621.6 0 21 20 13 621.6 0 13 21 19 621.6 0 19 22 21 621.6 0 21 23 16 621.6 0 16 24 13 621.6 0 13 25 16 621.6 0 16 26 24 621.6 0 24 27 21 621.6 0 21 28 23 621.6 0 23 29 19 621.6 0 19 30 23 621.6 0 23 31 22 621.6 0 22 32 19 621.6 0 19 33 26 621.6 0 26 34 24 621.6 0 24 35 22 621.6 0 22 36 28 621.6 0 28 37 16 621.6 0 16 38 16 621.6 0 16 39 20 621.6 0 20 40 35 621.6 0 35 41 22 621.6 0 22 42 22 621.6 0 22 43 21 621.6 0 21 44 22 621.6 0 22 45 14 621.6 0 14 46 23 621.6 0 23 47 23 621.6 0 23 48 21 621.6 0 21 49 10 621.6 0 10 50 22 621.6 0 22 51 10 621.6 0 10 52 14 621.6 0 14 53 20 621.6 0 20 54 10 621.6 0 10 55 34 621.6 0 34 56 32 621.6 0 32 57 34 621.6 0 34 58 29 621.6 0 29 59 25 621.6 0 25 60 21 621.6 0 21 61 21 621.6 0 21 62 16 621.6 0 16 63 27 621.6 0 27 64 16 621.6 0 16 65 29 621.6 0 29 66 30 621.6 0 30 67 19 621.6 0 19 68 26 621.6 0 26 69 15 621.6 0 15 70 11 621.6 0 11 71 21 621.6 0 21 72 17 621.6 0 17 73 28 621.6 0 28 74 26 621.6 0 26 75 54 621.6 0 54 76 24 621.6 0 24 77 28 621.6 0 28 78 16 621.6 0 16 79 26 621.6 0 26 80 18 621.6 0 18 81 24 621.6 0 24 82 17 621.6 0 17 83 22 621.6 0 22 84 36 621.6 0 36 85 18 621.6 0 18 86 18 621.6 0 18 87 33 621.6 0 33 88 24 621.6 0 24 89 33 621.6 0 33 90 33 621.6 0 33 91 22 621.6 0 22 92 23 621.6 0 23 93 18 621.6 0 18 94 30 621.6 0 30 95 28 621.6 0 28 96 19 621.6 0 19 97 20 621.6 0 20 98 22 621.6 0 22 99 20 621.6 0 20 100 12 621.6 0 12 101 28 621.6 0 28 102 18 621.6 0 18 103 62 621.6 0 62 104 50 621.6 0 50 105 115 621.6 0 115 106 133 621.6 0 133 107 128 621.6 0 128 108 14 621.6 0 14 109 39 621.6 0 39 110 44 621.6 0 44 111 44 621.6 0 44 112 39 621.6 0 39 113 46 621.6 0 46 114 47 621.6 0 47 115 44 621.6 0 44 116 49 621.6 0 49 117 37 621.6 0 37 118 46 621.6 0 46 119 21 621.6 0 21 120 29 621.6 0 29 121 40 621.6 0 40 122 36 621.6 0 36 123 47 621.6 0 47 124 36 621.6 0 36 125 48 621.6 0 48 126 106 621.6 0 106 127 237 621.6 0 237 128 858 621.6 0 858 129 355 621.6 0 355 130 596 621.6 0 596 131 67 621.6 0 67 132 79 621.6 0 79 133 106 621.6 0 106 134 154 621.6 0 154 135 60 621.6 0 60 136 77 621.6 0 77 137 198 621.6 0 198 138 63 621.6 0 63 139 59 621.6 0 59 140 104 621.6 0 104 141 88 621.6 0 88 142 165 621.6 0 165 143 103 621.6 0 103 144 174 621.6 0 174 145 182 621.6 0 182 146 201 621.6 0 201 147 4176 621.6 0 4176 148 3643 621.6 0 3643 149 8465 621.6 0 8465 150 116 621.6 0 116 151 32 621.6 0 32 152 96 621.6 0 96 153 41 621.6 0 41 154 109 621.6 0 109 155 48 621.6 0 48 156 43 621.6 0 43 157 40 621.6 0 40 158 35 621.6 0 35 159 39 621.6 0 39 160 34 621.6 0 34 161 37 621.6 0 37 162 36 621.6 0 36 163 24 621.6 0 24 164 42 621.6 0 42 165 27 621.6 0 27 166 27 621.6 0 27 167 24 621.6 0 24 168 54 621.6 0 54 169 105 621.6 0 105 170 70 621.6 0 70 171 83 621.6 0 83 172 168 621.6 0 168 173 108 621.6 0 108 174 151 621.6 0 151 175 37 621.6 0 37 176 31 621.6 0 31 177 55 621.6 0 55 178 40 621.6 0 40 179 35 621.6 0 35 180 37 621.6 0 37 181 36 621.6 0 36 182 29 621.6 0 29 183 74 621.6 0 74 184 96 621.6 0 96 185 34 621.6 0 34 186 58 621.6 0 58 187 179 621.6 0 179 188 1291 621.6 0 1291 189 986 621.6 0 986 190 1786 621.6 0 1786 191 50 621.6 0 50 192 25 621.6 0 25 193 37 621.6 0 37 194 36 621.6 0 36 195 26 621.6 0 26 196 71 621.6 0 71 197 104 621.6 0 104 198 89 621.6 0 89 199 161 621.6 0 161 200 496 621.6 0 496 201 3144 621.6 0 3144 202 1927 621.6 0 1927 203 3533 621.6 0 3533 204 182 621.6 0 182 205 26 621.6 0 26 206 22 621.6 0 22 207 26 621.6 0 26 208 36 621.6 0 36 209 13 621.6 0 13 210 27 621.6 0 27 211 25 621.6 0 25 212 24 621.6 0 24 213 20 621.6 0 20 214 14 621.6 0 14 215 28 621.6 0 28 216 25 621.6 0 25 217 24 621.6 0 24 218 17 621.6 0 17 219 15 621.6 0 15 220 24 621.6 0 24 221 18 621.6 0 18 222 25 621.6 0 25 223 19 621.6 0 19 224 16 621.6 0 16 225 44 621.6 0 44 226 61 621.6 0 61 227 39 621.6 0 39 228 88 621.6 0 88 229 304 621.6 0 304 230 72 621.6 0 72 231 24 621.6 0 24 232 29 621.6 0 29 233 18 621.6 0 18 234 23 621.6 0 23 235 22 621.6 0 22 236 45 621.6 0 45 237 29 621.6 0 29 238 34 621.6 0 34 239 22 621.6 0 22 240 29 621.6 0 29 241 28 621.6 0 28 242 31 621.6 0 31 243 38 621.6 0 38 244 31 621.6 0 31 245 26 621.6 0 26 246 21 621.6 0 21 247 17 621.6 0 17 248 18 621.6 0 18 249 47 621.6 0 47 250 47 621.6 0 47 251 50 621.6 0 50 252 63 621.6 0 63 253 21 621.6 0 21 254 26 621.6 0 26 255 17 621.6 0 17 256 18 621.6 0 18 257 16 621.6 0 16 258 41 621.6 0 41 259 39 621.6 0 39 260 59 621.6 0 59 261 62 621.6 0 62 262 40 621.6 0 40 263 38 621.6 0 38 264 18 621.6 0 18 265 31 621.6 0 31 266 28 621.6 0 28 267 37 621.6 0 37 268 41 621.6 0 41 269 220 621.6 0 220 270 85 621.6 0 85 271 124 621.6 0 124 272 51 621.6 0 51 273 35 621.6 0 35 274 26 621.6 0 26 275 56 621.6 0 56 276 71 621.6 0 71 277 44 621.6 0 44 278 45 621.6 0 45 279 64 621.6 0 64 280 104 621.6 0 104 281 45 621.6 0 45 282 162 621.6 0 162 283 75 621.6 0 75 284 101 621.6 0 101 285 23 621.6 0 23 286 32 621.6 0 32 287 61 621.6 0 61 288 37 621.6 0 37 289 37 621.6 0 37 290 32 621.6 0 32 291 145 621.6 0 145 292 91 621.6 0 91 293 166 621.6 0 166 294 183 621.6 0 183 295 131 621.6 0 131 296 162 621.6 0 162 297 66 621.6 0 66 298 18 621.6 0 18 299 55 621.6 0 55 300 51 621.6 0 51 301 38 621.6 0 38 Finished in 1.84 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 47,985 Reads with adapters: 6,432 (13.4%) Reads that were too short: 29 (0.1%) Reads written (passing filters): 6,403 (13.3%) Total basepairs processed: 9,119,936 bp Total written (filtered): 1,762,121 bp (19.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6432 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 32 0.0 3 0 31 1 17 4905 0.0 3 4806 98 1 18 22 0.0 3 0 22 19 1384 0.0 3 1347 21 4 12 20 2 0.0 3 0 1 1 21 2 0.0 3 1 0 1 22 7 0.0 3 0 0 0 7 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 45 21 0.0 3 0 0 0 21 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 63 12 0.0 3 0 0 0 12 64 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 79 11 0.0 3 0 0 1 10 83 1 0.0 3 1 87 5 0.0 3 0 0 0 5 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 228 1 0.0 3 1 229 1 0.0 3 0 0 0 1 231 1 0.0 3 0 1 232 1 0.0 3 1 248 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 6,403 Reads with adapters: 35 (0.5%) Reads written (passing filters): 6,403 (100.0%) Total basepairs processed: 1,762,121 bp Total written (filtered): 1,761,964 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.3% C: 17.1% G: 25.7% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 100.0 0 12 4 8 25.0 0 8 5 1 6.3 0 1 6 14 1.6 0 14