This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.29 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 80,651 (3.2%) Reads that were too short: 39,663 (1.6%) Reads written (passing filters): 40,988 (1.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 8,227,957 bp (1.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 80651 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 576 621.6 0 576 7 5198 621.6 0 5198 8 129 621.6 0 129 9 5224 621.6 0 5224 10 26 621.6 0 26 11 36 621.6 0 36 12 28 621.6 0 28 13 22 621.6 0 22 14 22 621.6 0 22 15 31 621.6 0 31 16 20 621.6 0 20 17 41 621.6 0 41 18 21 621.6 0 21 19 19 621.6 0 19 20 15 621.6 0 15 21 17 621.6 0 17 22 21 621.6 0 21 23 33 621.6 0 33 24 26 621.6 0 26 25 31 621.6 0 31 26 29 621.6 0 29 27 42 621.6 0 42 28 46 621.6 0 46 29 36 621.6 0 36 30 33 621.6 0 33 31 23 621.6 0 23 32 26 621.6 0 26 33 26 621.6 0 26 34 24 621.6 0 24 35 21 621.6 0 21 36 26 621.6 0 26 37 26 621.6 0 26 38 29 621.6 0 29 39 19 621.6 0 19 40 24 621.6 0 24 41 235 621.6 0 235 42 161 621.6 0 161 43 742 621.6 0 742 44 47 621.6 0 47 45 36 621.6 0 36 46 26 621.6 0 26 47 29 621.6 0 29 48 29 621.6 0 29 49 29 621.6 0 29 50 38 621.6 0 38 51 35 621.6 0 35 52 50 621.6 0 50 53 14 621.6 0 14 54 19 621.6 0 19 55 22 621.6 0 22 56 25 621.6 0 25 57 38 621.6 0 38 58 46 621.6 0 46 59 39 621.6 0 39 60 32 621.6 0 32 61 30 621.6 0 30 62 38 621.6 0 38 63 37 621.6 0 37 64 23 621.6 0 23 65 34 621.6 0 34 66 36 621.6 0 36 67 43 621.6 0 43 68 23 621.6 0 23 69 42 621.6 0 42 70 28 621.6 0 28 71 49 621.6 0 49 72 96 621.6 0 96 73 80 621.6 0 80 74 125 621.6 0 125 75 79 621.6 0 79 76 81 621.6 0 81 77 79 621.6 0 79 78 48 621.6 0 48 79 53 621.6 0 53 80 71 621.6 0 71 81 56 621.6 0 56 82 129 621.6 0 129 83 61 621.6 0 61 84 45 621.6 0 45 85 47 621.6 0 47 86 24 621.6 0 24 87 25 621.6 0 25 88 18 621.6 0 18 89 22 621.6 0 22 90 37 621.6 0 37 91 33 621.6 0 33 92 38 621.6 0 38 93 56 621.6 0 56 94 52 621.6 0 52 95 53 621.6 0 53 96 40 621.6 0 40 97 57 621.6 0 57 98 39 621.6 0 39 99 61 621.6 0 61 100 63 621.6 0 63 101 99 621.6 0 99 102 131 621.6 0 131 103 157 621.6 0 157 104 240 621.6 0 240 105 295 621.6 0 295 106 300 621.6 0 300 107 310 621.6 0 310 108 255 621.6 0 255 109 222 621.6 0 222 110 181 621.6 0 181 111 191 621.6 0 191 112 182 621.6 0 182 113 219 621.6 0 219 114 215 621.6 0 215 115 179 621.6 0 179 116 223 621.6 0 223 117 167 621.6 0 167 118 220 621.6 0 220 119 213 621.6 0 213 120 265 621.6 0 265 121 162 621.6 0 162 122 166 621.6 0 166 123 145 621.6 0 145 124 229 621.6 0 229 125 224 621.6 0 224 126 265 621.6 0 265 127 152 621.6 0 152 128 165 621.6 0 165 129 129 621.6 0 129 130 158 621.6 0 158 131 212 621.6 0 212 132 259 621.6 0 259 133 1803 621.6 0 1803 134 794 621.6 0 794 135 1832 621.6 0 1832 136 163 621.6 0 163 137 148 621.6 0 148 138 126 621.6 0 126 139 120 621.6 0 120 140 152 621.6 0 152 141 152 621.6 0 152 142 147 621.6 0 147 143 170 621.6 0 170 144 178 621.6 0 178 145 178 621.6 0 178 146 151 621.6 0 151 147 322 621.6 0 322 148 157 621.6 0 157 149 175 621.6 0 175 150 109 621.6 0 109 151 126 621.6 0 126 152 112 621.6 0 112 153 99 621.6 0 99 154 150 621.6 0 150 155 140 621.6 0 140 156 183 621.6 0 183 157 228 621.6 0 228 158 303 621.6 0 303 159 398 621.6 0 398 160 383 621.6 0 383 161 374 621.6 0 374 162 955 621.6 0 955 163 497 621.6 0 497 164 839 621.6 0 839 165 235 621.6 0 235 166 267 621.6 0 267 167 219 621.6 0 219 168 220 621.6 0 220 169 224 621.6 0 224 170 197 621.6 0 197 171 251 621.6 0 251 172 224 621.6 0 224 173 233 621.6 0 233 174 138 621.6 0 138 175 131 621.6 0 131 176 90 621.6 0 90 177 158 621.6 0 158 178 205 621.6 0 205 179 206 621.6 0 206 180 277 621.6 0 277 181 186 621.6 0 186 182 224 621.6 0 224 183 248 621.6 0 248 184 194 621.6 0 194 185 218 621.6 0 218 186 177 621.6 0 177 187 175 621.6 0 175 188 154 621.6 0 154 189 339 621.6 0 339 190 172 621.6 0 172 191 179 621.6 0 179 192 230 621.6 0 230 193 146 621.6 0 146 194 125 621.6 0 125 195 150 621.6 0 150 196 137 621.6 0 137 197 129 621.6 0 129 198 141 621.6 0 141 199 129 621.6 0 129 200 159 621.6 0 159 201 198 621.6 0 198 202 167 621.6 0 167 203 135 621.6 0 135 204 137 621.6 0 137 205 203 621.6 0 203 206 196 621.6 0 196 207 208 621.6 0 208 208 215 621.6 0 215 209 226 621.6 0 226 210 171 621.6 0 171 211 283 621.6 0 283 212 599 621.6 0 599 213 3902 621.6 0 3902 214 2134 621.6 0 2134 215 2303 621.6 0 2303 216 462 621.6 0 462 217 280 621.6 0 280 218 309 621.6 0 309 219 226 621.6 0 226 220 211 621.6 0 211 221 266 621.6 0 266 222 259 621.6 0 259 223 328 621.6 0 328 224 540 621.6 0 540 225 1247 621.6 0 1247 226 1310 621.6 0 1310 227 1627 621.6 0 1627 228 382 621.6 0 382 229 335 621.6 0 335 230 247 621.6 0 247 231 225 621.6 0 225 232 193 621.6 0 193 233 177 621.6 0 177 234 191 621.6 0 191 235 199 621.6 0 199 236 217 621.6 0 217 237 243 621.6 0 243 238 214 621.6 0 214 239 195 621.6 0 195 240 171 621.6 0 171 241 186 621.6 0 186 242 210 621.6 0 210 243 190 621.6 0 190 244 162 621.6 0 162 245 144 621.6 0 144 246 167 621.6 0 167 247 218 621.6 0 218 248 222 621.6 0 222 249 321 621.6 0 321 250 499 621.6 0 499 251 353 621.6 0 353 252 526 621.6 0 526 253 431 621.6 0 431 254 501 621.6 0 501 255 384 621.6 0 384 256 252 621.6 0 252 257 204 621.6 0 204 258 211 621.6 0 211 259 205 621.6 0 205 260 217 621.6 0 217 261 213 621.6 0 213 262 283 621.6 0 283 263 365 621.6 0 365 264 403 621.6 0 403 265 442 621.6 0 442 266 395 621.6 0 395 267 273 621.6 0 273 268 310 621.6 0 310 269 352 621.6 0 352 270 430 621.6 0 430 271 351 621.6 0 351 272 301 621.6 0 301 273 254 621.6 0 254 274 277 621.6 0 277 275 279 621.6 0 279 276 352 621.6 0 352 277 372 621.6 0 372 278 349 621.6 0 349 279 294 621.6 0 294 280 259 621.6 0 259 281 306 621.6 0 306 282 320 621.6 0 320 283 335 621.6 0 335 284 388 621.6 0 388 285 376 621.6 0 376 286 283 621.6 0 283 287 275 621.6 0 275 288 254 621.6 0 254 289 358 621.6 0 358 290 395 621.6 0 395 291 400 621.6 0 400 292 304 621.6 0 304 293 256 621.6 0 256 294 269 621.6 0 269 295 309 621.6 0 309 296 304 621.6 0 304 297 242 621.6 0 242 298 224 621.6 0 224 299 249 621.6 0 249 300 255 621.6 0 255 301 471 621.6 0 471 Finished in 1.79 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 40,988 Reads with adapters: 5,660 (13.8%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 5,624 (13.7%) Total basepairs processed: 8,227,957 bp Total written (filtered): 1,535,982 bp (18.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5660 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 114 0.0 3 0 98 8 4 4 20 4993 0.0 4 4655 268 53 14 3 21 16 0.0 4 2 12 1 1 22 436 0.0 4 386 33 14 1 2 23 18 0.0 4 0 2 0 14 2 24 1 0.0 4 0 0 0 0 1 25 4 0.0 4 0 0 0 1 3 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 46 2 0.0 4 0 0 0 0 2 48 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 62 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 88 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 31 0.0 4 0 0 0 0 31 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 1 159 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 5,624 Reads with adapters: 343 (6.1%) Reads written (passing filters): 5,624 (100.0%) Total basepairs processed: 1,535,982 bp Total written (filtered): 1,531,799 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 343 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.7% C: 11.7% G: 77.8% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 29 87.9 0 29 4 4 22.0 0 4 10 25 0.0 1 2 23 11 202 0.0 1 5 197 12 78 0.0 1 7 71 13 2 0.0 1 1 1 210 1 0.0 1 1 211 1 0.0 1 0 1 225 1 0.0 1 1