This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.56 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 69,700 (2.7%) Reads that were too short: 33,781 (1.3%) Reads written (passing filters): 35,919 (1.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 7,494,072 bp (1.0%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 69700 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 642 621.6 0 642 7 5861 621.6 0 5861 8 99 621.6 0 99 9 4986 621.6 0 4986 10 32 621.6 0 32 11 19 621.6 0 19 12 28 621.6 0 28 13 17 621.6 0 17 14 20 621.6 0 20 15 29 621.6 0 29 16 32 621.6 0 32 17 28 621.6 0 28 18 17 621.6 0 17 19 30 621.6 0 30 20 21 621.6 0 21 21 22 621.6 0 22 22 36 621.6 0 36 23 25 621.6 0 25 24 9 621.6 0 9 25 31 621.6 0 31 26 31 621.6 0 31 27 15 621.6 0 15 28 43 621.6 0 43 29 27 621.6 0 27 30 37 621.6 0 37 31 20 621.6 0 20 32 35 621.6 0 35 33 22 621.6 0 22 34 22 621.6 0 22 35 14 621.6 0 14 36 24 621.6 0 24 37 15 621.6 0 15 38 27 621.6 0 27 39 21 621.6 0 21 40 40 621.6 0 40 41 128 621.6 0 128 42 92 621.6 0 92 43 169 621.6 0 169 44 23 621.6 0 23 45 22 621.6 0 22 46 34 621.6 0 34 47 31 621.6 0 31 48 35 621.6 0 35 49 28 621.6 0 28 50 31 621.6 0 31 51 40 621.6 0 40 52 40 621.6 0 40 53 24 621.6 0 24 54 16 621.6 0 16 55 20 621.6 0 20 56 30 621.6 0 30 57 28 621.6 0 28 58 30 621.6 0 30 59 31 621.6 0 31 60 29 621.6 0 29 61 25 621.6 0 25 62 35 621.6 0 35 63 36 621.6 0 36 64 26 621.6 0 26 65 20 621.6 0 20 66 21 621.6 0 21 67 29 621.6 0 29 68 19 621.6 0 19 69 53 621.6 0 53 70 51 621.6 0 51 71 34 621.6 0 34 72 46 621.6 0 46 73 44 621.6 0 44 74 78 621.6 0 78 75 38 621.6 0 38 76 58 621.6 0 58 77 77 621.6 0 77 78 66 621.6 0 66 79 139 621.6 0 139 80 166 621.6 0 166 81 151 621.6 0 151 82 165 621.6 0 165 83 185 621.6 0 185 84 179 621.6 0 179 85 138 621.6 0 138 86 97 621.6 0 97 87 76 621.6 0 76 88 84 621.6 0 84 89 76 621.6 0 76 90 76 621.6 0 76 91 106 621.6 0 106 92 88 621.6 0 88 93 97 621.6 0 97 94 59 621.6 0 59 95 90 621.6 0 90 96 87 621.6 0 87 97 77 621.6 0 77 98 74 621.6 0 74 99 73 621.6 0 73 100 77 621.6 0 77 101 111 621.6 0 111 102 118 621.6 0 118 103 114 621.6 0 114 104 144 621.6 0 144 105 158 621.6 0 158 106 194 621.6 0 194 107 172 621.6 0 172 108 144 621.6 0 144 109 149 621.6 0 149 110 170 621.6 0 170 111 159 621.6 0 159 112 160 621.6 0 160 113 182 621.6 0 182 114 158 621.6 0 158 115 156 621.6 0 156 116 251 621.6 0 251 117 164 621.6 0 164 118 178 621.6 0 178 119 190 621.6 0 190 120 198 621.6 0 198 121 148 621.6 0 148 122 139 621.6 0 139 123 157 621.6 0 157 124 174 621.6 0 174 125 198 621.6 0 198 126 318 621.6 0 318 127 152 621.6 0 152 128 134 621.6 0 134 129 139 621.6 0 139 130 164 621.6 0 164 131 188 621.6 0 188 132 237 621.6 0 237 133 1327 621.6 0 1327 134 750 621.6 0 750 135 1168 621.6 0 1168 136 142 621.6 0 142 137 130 621.6 0 130 138 139 621.6 0 139 139 138 621.6 0 138 140 129 621.6 0 129 141 127 621.6 0 127 142 144 621.6 0 144 143 130 621.6 0 130 144 161 621.6 0 161 145 149 621.6 0 149 146 159 621.6 0 159 147 329 621.6 0 329 148 159 621.6 0 159 149 139 621.6 0 139 150 138 621.6 0 138 151 121 621.6 0 121 152 125 621.6 0 125 153 138 621.6 0 138 154 128 621.6 0 128 155 148 621.6 0 148 156 140 621.6 0 140 157 148 621.6 0 148 158 217 621.6 0 217 159 236 621.6 0 236 160 207 621.6 0 207 161 183 621.6 0 183 162 351 621.6 0 351 163 265 621.6 0 265 164 357 621.6 0 357 165 164 621.6 0 164 166 132 621.6 0 132 167 132 621.6 0 132 168 132 621.6 0 132 169 141 621.6 0 141 170 144 621.6 0 144 171 211 621.6 0 211 172 187 621.6 0 187 173 168 621.6 0 168 174 134 621.6 0 134 175 147 621.6 0 147 176 111 621.6 0 111 177 117 621.6 0 117 178 156 621.6 0 156 179 191 621.6 0 191 180 169 621.6 0 169 181 153 621.6 0 153 182 168 621.6 0 168 183 153 621.6 0 153 184 119 621.6 0 119 185 197 621.6 0 197 186 168 621.6 0 168 187 144 621.6 0 144 188 151 621.6 0 151 189 318 621.6 0 318 190 157 621.6 0 157 191 181 621.6 0 181 192 182 621.6 0 182 193 178 621.6 0 178 194 114 621.6 0 114 195 116 621.6 0 116 196 148 621.6 0 148 197 128 621.6 0 128 198 138 621.6 0 138 199 147 621.6 0 147 200 142 621.6 0 142 201 167 621.6 0 167 202 148 621.6 0 148 203 158 621.6 0 158 204 135 621.6 0 135 205 137 621.6 0 137 206 132 621.6 0 132 207 165 621.6 0 165 208 177 621.6 0 177 209 202 621.6 0 202 210 222 621.6 0 222 211 364 621.6 0 364 212 744 621.6 0 744 213 6614 621.6 0 6614 214 2717 621.6 0 2717 215 3288 621.6 0 3288 216 302 621.6 0 302 217 213 621.6 0 213 218 174 621.6 0 174 219 187 621.6 0 187 220 139 621.6 0 139 221 160 621.6 0 160 222 185 621.6 0 185 223 219 621.6 0 219 224 250 621.6 0 250 225 511 621.6 0 511 226 597 621.6 0 597 227 788 621.6 0 788 228 252 621.6 0 252 229 211 621.6 0 211 230 176 621.6 0 176 231 195 621.6 0 195 232 150 621.6 0 150 233 161 621.6 0 161 234 153 621.6 0 153 235 164 621.6 0 164 236 155 621.6 0 155 237 168 621.6 0 168 238 166 621.6 0 166 239 141 621.6 0 141 240 134 621.6 0 134 241 153 621.6 0 153 242 155 621.6 0 155 243 170 621.6 0 170 244 154 621.6 0 154 245 137 621.6 0 137 246 150 621.6 0 150 247 185 621.6 0 185 248 195 621.6 0 195 249 211 621.6 0 211 250 401 621.6 0 401 251 251 621.6 0 251 252 339 621.6 0 339 253 233 621.6 0 233 254 220 621.6 0 220 255 182 621.6 0 182 256 181 621.6 0 181 257 172 621.6 0 172 258 150 621.6 0 150 259 165 621.6 0 165 260 189 621.6 0 189 261 178 621.6 0 178 262 180 621.6 0 180 263 184 621.6 0 184 264 220 621.6 0 220 265 216 621.6 0 216 266 229 621.6 0 229 267 158 621.6 0 158 268 224 621.6 0 224 269 181 621.6 0 181 270 246 621.6 0 246 271 231 621.6 0 231 272 214 621.6 0 214 273 202 621.6 0 202 274 198 621.6 0 198 275 189 621.6 0 189 276 287 621.6 0 287 277 255 621.6 0 255 278 261 621.6 0 261 279 232 621.6 0 232 280 199 621.6 0 199 281 223 621.6 0 223 282 245 621.6 0 245 283 232 621.6 0 232 284 236 621.6 0 236 285 213 621.6 0 213 286 189 621.6 0 189 287 194 621.6 0 194 288 170 621.6 0 170 289 232 621.6 0 232 290 288 621.6 0 288 291 251 621.6 0 251 292 204 621.6 0 204 293 160 621.6 0 160 294 181 621.6 0 181 295 189 621.6 0 189 296 161 621.6 0 161 297 181 621.6 0 181 298 138 621.6 0 138 299 159 621.6 0 159 300 198 621.6 0 198 301 306 621.6 0 306 Finished in 1.44 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 35,919 Reads with adapters: 5,176 (14.4%) Reads that were too short: 28 (0.1%) Reads written (passing filters): 5,148 (14.3%) Total basepairs processed: 7,494,072 bp Total written (filtered): 1,412,925 bp (18.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5176 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 31 0.0 3 0 29 1 1 17 5076 0.0 3 4989 84 3 18 12 0.0 3 0 12 19 6 0.0 3 1 0 3 2 20 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 1 202 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 5,148 Reads with adapters: 65 (1.3%) Reads written (passing filters): 5,148 (100.0%) Total basepairs processed: 1,412,925 bp Total written (filtered): 1,412,726 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 65 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 6.2% G: 32.3% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 61 80.4 0 61 4 4 20.1 0 4