This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.70 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 376,126 (14.8%) Reads that were too short: 319,354 (12.5%) Reads written (passing filters): 56,772 (2.2%) Total basepairs processed: 766,381,518 bp Total written (filtered): 13,041,318 bp (1.7%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 376126 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 330 621.6 0 330 7 502 621.6 0 502 8 14331 621.6 0 14331 9 14815 621.6 0 14815 10 99 621.6 0 99 11 87 621.6 0 87 12 75 621.6 0 75 13 45 621.6 0 45 14 83 621.6 0 83 15 49 621.6 0 49 16 74 621.6 0 74 17 95 621.6 0 95 18 87 621.6 0 87 19 64 621.6 0 64 20 78 621.6 0 78 21 66 621.6 0 66 22 58 621.6 0 58 23 75 621.6 0 75 24 55 621.6 0 55 25 53 621.6 0 53 26 66 621.6 0 66 27 69 621.6 0 69 28 61 621.6 0 61 29 60 621.6 0 60 30 49 621.6 0 49 31 58 621.6 0 58 32 39 621.6 0 39 33 56 621.6 0 56 34 61 621.6 0 61 35 65 621.6 0 65 36 79 621.6 0 79 37 77 621.6 0 77 38 59 621.6 0 59 39 85 621.6 0 85 40 56 621.6 0 56 41 62 621.6 0 62 42 60 621.6 0 60 43 60 621.6 0 60 44 58 621.6 0 58 45 59 621.6 0 59 46 81 621.6 0 81 47 73 621.6 0 73 48 84 621.6 0 84 49 68 621.6 0 68 50 62 621.6 0 62 51 69 621.6 0 69 52 49 621.6 0 49 53 57 621.6 0 57 54 48 621.6 0 48 55 63 621.6 0 63 56 61 621.6 0 61 57 89 621.6 0 89 58 65 621.6 0 65 59 33 621.6 0 33 60 66 621.6 0 66 61 64 621.6 0 64 62 49 621.6 0 49 63 104 621.6 0 104 64 112 621.6 0 112 65 65 621.6 0 65 66 117 621.6 0 117 67 110 621.6 0 110 68 66 621.6 0 66 69 78 621.6 0 78 70 68 621.6 0 68 71 96 621.6 0 96 72 59 621.6 0 59 73 67 621.6 0 67 74 76 621.6 0 76 75 87 621.6 0 87 76 60 621.6 0 60 77 77 621.6 0 77 78 79 621.6 0 79 79 81 621.6 0 81 80 49 621.6 0 49 81 66 621.6 0 66 82 98 621.6 0 98 83 63 621.6 0 63 84 65 621.6 0 65 85 40 621.6 0 40 86 87 621.6 0 87 87 71 621.6 0 71 88 96 621.6 0 96 89 96 621.6 0 96 90 84 621.6 0 84 91 90 621.6 0 90 92 99 621.6 0 99 93 118 621.6 0 118 94 67 621.6 0 67 95 68 621.6 0 68 96 56 621.6 0 56 97 75 621.6 0 75 98 69 621.6 0 69 99 88 621.6 0 88 100 71 621.6 0 71 101 59 621.6 0 59 102 93 621.6 0 93 103 81 621.6 0 81 104 63 621.6 0 63 105 76 621.6 0 76 106 90 621.6 0 90 107 70 621.6 0 70 108 83 621.6 0 83 109 82 621.6 0 82 110 70 621.6 0 70 111 77 621.6 0 77 112 64 621.6 0 64 113 80 621.6 0 80 114 70 621.6 0 70 115 51 621.6 0 51 116 98 621.6 0 98 117 96 621.6 0 96 118 74 621.6 0 74 119 93 621.6 0 93 120 87 621.6 0 87 121 77 621.6 0 77 122 77 621.6 0 77 123 105 621.6 0 105 124 103 621.6 0 103 125 109 621.6 0 109 126 151 621.6 0 151 127 124 621.6 0 124 128 96 621.6 0 96 129 89 621.6 0 89 130 206 621.6 0 206 131 363 621.6 0 363 132 408 621.6 0 408 133 460 621.6 0 460 134 98 621.6 0 98 135 79 621.6 0 79 136 82 621.6 0 82 137 107 621.6 0 107 138 94 621.6 0 94 139 75 621.6 0 75 140 106 621.6 0 106 141 122 621.6 0 122 142 89 621.6 0 89 143 96 621.6 0 96 144 109 621.6 0 109 145 103 621.6 0 103 146 116 621.6 0 116 147 125 621.6 0 125 148 131 621.6 0 131 149 112 621.6 0 112 150 109 621.6 0 109 151 111 621.6 0 111 152 137 621.6 0 137 153 137 621.6 0 137 154 162 621.6 0 162 155 150 621.6 0 150 156 121 621.6 0 121 157 125 621.6 0 125 158 139 621.6 0 139 159 110 621.6 0 110 160 123 621.6 0 123 161 125 621.6 0 125 162 113 621.6 0 113 163 129 621.6 0 129 164 130 621.6 0 130 165 156 621.6 0 156 166 134 621.6 0 134 167 157 621.6 0 157 168 169 621.6 0 169 169 145 621.6 0 145 170 148 621.6 0 148 171 146 621.6 0 146 172 140 621.6 0 140 173 205 621.6 0 205 174 273 621.6 0 273 175 296 621.6 0 296 176 211 621.6 0 211 177 167 621.6 0 167 178 141 621.6 0 141 179 207 621.6 0 207 180 202 621.6 0 202 181 324 621.6 0 324 182 190 621.6 0 190 183 152 621.6 0 152 184 172 621.6 0 172 185 297 621.6 0 297 186 342 621.6 0 342 187 440 621.6 0 440 188 281 621.6 0 281 189 216 621.6 0 216 190 222 621.6 0 222 191 270 621.6 0 270 192 346 621.6 0 346 193 1510 621.6 0 1510 194 866 621.6 0 866 195 2356 621.6 0 2356 196 188 621.6 0 188 197 190 621.6 0 190 198 187 621.6 0 187 199 455 621.6 0 455 200 481 621.6 0 481 201 441 621.6 0 441 202 247 621.6 0 247 203 172 621.6 0 172 204 166 621.6 0 166 205 212 621.6 0 212 206 212 621.6 0 212 207 231 621.6 0 231 208 185 621.6 0 185 209 152 621.6 0 152 210 248 621.6 0 248 211 297 621.6 0 297 212 234 621.6 0 234 213 399 621.6 0 399 214 271 621.6 0 271 215 239 621.6 0 239 216 255 621.6 0 255 217 239 621.6 0 239 218 245 621.6 0 245 219 375 621.6 0 375 220 345 621.6 0 345 221 386 621.6 0 386 222 473 621.6 0 473 223 735 621.6 0 735 224 1038 621.6 0 1038 225 403 621.6 0 403 226 458 621.6 0 458 227 714 621.6 0 714 228 432 621.6 0 432 229 460 621.6 0 460 230 802 621.6 0 802 231 1074 621.6 0 1074 232 3530 621.6 0 3530 233 4111 621.6 0 4111 234 6680 621.6 0 6680 235 1922 621.6 0 1922 236 1928 621.6 0 1928 237 43403 621.6 0 43403 238 27241 621.6 0 27241 239 49743 621.6 0 49743 240 1598 621.6 0 1598 241 806 621.6 0 806 242 755 621.6 0 755 243 699 621.6 0 699 244 971 621.6 0 971 245 516 621.6 0 516 246 436 621.6 0 436 247 679 621.6 0 679 248 541 621.6 0 541 249 614 621.6 0 614 250 787 621.6 0 787 251 858 621.6 0 858 252 1396 621.6 0 1396 253 1238 621.6 0 1238 254 1828 621.6 0 1828 255 2139 621.6 0 2139 256 1946 621.6 0 1946 257 38840 621.6 0 38840 258 37885 621.6 0 37885 259 44141 621.6 0 44141 260 21413 621.6 0 21413 261 244 621.6 0 244 262 194 621.6 0 194 263 178 621.6 0 178 264 163 621.6 0 163 265 170 621.6 0 170 266 152 621.6 0 152 267 170 621.6 0 170 268 215 621.6 0 215 269 160 621.6 0 160 270 191 621.6 0 191 271 157 621.6 0 157 272 177 621.6 0 177 273 159 621.6 0 159 274 159 621.6 0 159 275 153 621.6 0 153 276 179 621.6 0 179 277 156 621.6 0 156 278 144 621.6 0 144 279 176 621.6 0 176 280 162 621.6 0 162 281 162 621.6 0 162 282 189 621.6 0 189 283 192 621.6 0 192 284 178 621.6 0 178 285 192 621.6 0 192 286 180 621.6 0 180 287 219 621.6 0 219 288 184 621.6 0 184 289 207 621.6 0 207 290 271 621.6 0 271 291 576 621.6 0 576 292 585 621.6 0 585 293 759 621.6 0 759 294 362 621.6 0 362 295 207 621.6 0 207 296 191 621.6 0 191 297 195 621.6 0 195 298 161 621.6 0 161 299 155 621.6 0 155 300 185 621.6 0 185 301 202 621.6 0 202 Finished in 2.46 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 56,772 Reads with adapters: 13,716 (24.2%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 13,694 (24.1%) Total basepairs processed: 13,041,318 bp Total written (filtered): 3,778,095 bp (29.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13716 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 94 0.0 3 0 89 5 17 13531 0.0 3 13262 265 2 2 18 30 0.0 3 0 27 0 3 19 13 0.0 3 4 0 8 1 20 11 0.0 3 0 0 0 11 34 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 1 102 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 13,694 Reads with adapters: 25 (0.2%) Reads written (passing filters): 13,694 (100.0%) Total basepairs processed: 3,778,095 bp Total written (filtered): 3,778,003 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 25 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.0% C: 12.0% G: 20.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 214.0 0 9 4 15 53.5 0 15 5 1 13.4 0 1