This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.01 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 54,060 (2.1%) Reads that were too short: 16,582 (0.7%) Reads written (passing filters): 37,478 (1.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 8,499,261 bp (1.1%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 54060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 300 621.6 0 300 7 7414 621.6 0 7414 8 268 621.6 0 268 9 8002 621.6 0 8002 10 26 621.6 0 26 11 23 621.6 0 23 12 17 621.6 0 17 13 15 621.6 0 15 14 24 621.6 0 24 15 18 621.6 0 18 16 30 621.6 0 30 17 37 621.6 0 37 18 50 621.6 0 50 19 8 621.6 0 8 20 25 621.6 0 25 21 14 621.6 0 14 22 33 621.6 0 33 23 33 621.6 0 33 24 34 621.6 0 34 25 33 621.6 0 33 26 504 621.6 0 504 27 209 621.6 0 209 28 493 621.6 0 493 29 84 621.6 0 84 30 148 621.6 0 148 31 151 621.6 0 151 32 10 621.6 0 10 33 11 621.6 0 11 34 22 621.6 0 22 35 27 621.6 0 27 36 24 621.6 0 24 37 13 621.6 0 13 38 14 621.6 0 14 39 29 621.6 0 29 40 17 621.6 0 17 41 9 621.6 0 9 42 14 621.6 0 14 43 16 621.6 0 16 44 13 621.6 0 13 45 24 621.6 0 24 46 14 621.6 0 14 47 20 621.6 0 20 48 17 621.6 0 17 49 27 621.6 0 27 50 21 621.6 0 21 51 28 621.6 0 28 52 25 621.6 0 25 53 20 621.6 0 20 54 19 621.6 0 19 55 19 621.6 0 19 56 26 621.6 0 26 57 29 621.6 0 29 58 25 621.6 0 25 59 29 621.6 0 29 60 28 621.6 0 28 61 31 621.6 0 31 62 25 621.6 0 25 63 23 621.6 0 23 64 24 621.6 0 24 65 37 621.6 0 37 66 18 621.6 0 18 67 45 621.6 0 45 68 46 621.6 0 46 69 397 621.6 0 397 70 124 621.6 0 124 71 488 621.6 0 488 72 28 621.6 0 28 73 25 621.6 0 25 74 23 621.6 0 23 75 30 621.6 0 30 76 28 621.6 0 28 77 70 621.6 0 70 78 78 621.6 0 78 79 52 621.6 0 52 80 21 621.6 0 21 81 34 621.6 0 34 82 34 621.6 0 34 83 44 621.6 0 44 84 39 621.6 0 39 85 34 621.6 0 34 86 43 621.6 0 43 87 72 621.6 0 72 88 36 621.6 0 36 89 25 621.6 0 25 90 61 621.6 0 61 91 30 621.6 0 30 92 35 621.6 0 35 93 39 621.6 0 39 94 28 621.6 0 28 95 24 621.6 0 24 96 48 621.6 0 48 97 79 621.6 0 79 98 91 621.6 0 91 99 68 621.6 0 68 100 22 621.6 0 22 101 35 621.6 0 35 102 43 621.6 0 43 103 18 621.6 0 18 104 31 621.6 0 31 105 28 621.6 0 28 106 19 621.6 0 19 107 26 621.6 0 26 108 32 621.6 0 32 109 19 621.6 0 19 110 32 621.6 0 32 111 40 621.6 0 40 112 70 621.6 0 70 113 43 621.6 0 43 114 177 621.6 0 177 115 109 621.6 0 109 116 169 621.6 0 169 117 25 621.6 0 25 118 44 621.6 0 44 119 29 621.6 0 29 120 30 621.6 0 30 121 37 621.6 0 37 122 46 621.6 0 46 123 53 621.6 0 53 124 37 621.6 0 37 125 31 621.6 0 31 126 59 621.6 0 59 127 105 621.6 0 105 128 114 621.6 0 114 129 89 621.6 0 89 130 113 621.6 0 113 131 68 621.6 0 68 132 269 621.6 0 269 133 105 621.6 0 105 134 229 621.6 0 229 135 142 621.6 0 142 136 83 621.6 0 83 137 130 621.6 0 130 138 324 621.6 0 324 139 398 621.6 0 398 140 1919 621.6 0 1919 141 3600 621.6 0 3600 142 1424 621.6 0 1424 143 448 621.6 0 448 144 1207 621.6 0 1207 145 296 621.6 0 296 146 72 621.6 0 72 147 116 621.6 0 116 148 220 621.6 0 220 149 259 621.6 0 259 150 325 621.6 0 325 151 271 621.6 0 271 152 159 621.6 0 159 153 322 621.6 0 322 154 29 621.6 0 29 155 43 621.6 0 43 156 23 621.6 0 23 157 39 621.6 0 39 158 25 621.6 0 25 159 38 621.6 0 38 160 27 621.6 0 27 161 25 621.6 0 25 162 31 621.6 0 31 163 23 621.6 0 23 164 22 621.6 0 22 165 22 621.6 0 22 166 25 621.6 0 25 167 24 621.6 0 24 168 24 621.6 0 24 169 25 621.6 0 25 170 30 621.6 0 30 171 28 621.6 0 28 172 22 621.6 0 22 173 67 621.6 0 67 174 41 621.6 0 41 175 65 621.6 0 65 176 37 621.6 0 37 177 31 621.6 0 31 178 31 621.6 0 31 179 26 621.6 0 26 180 39 621.6 0 39 181 27 621.6 0 27 182 42 621.6 0 42 183 26 621.6 0 26 184 84 621.6 0 84 185 62 621.6 0 62 186 88 621.6 0 88 187 49 621.6 0 49 188 47 621.6 0 47 189 169 621.6 0 169 190 123 621.6 0 123 191 265 621.6 0 265 192 82 621.6 0 82 193 48 621.6 0 48 194 42 621.6 0 42 195 29 621.6 0 29 196 65 621.6 0 65 197 50 621.6 0 50 198 48 621.6 0 48 199 41 621.6 0 41 200 28 621.6 0 28 201 30 621.6 0 30 202 45 621.6 0 45 203 59 621.6 0 59 204 59 621.6 0 59 205 59 621.6 0 59 206 41 621.6 0 41 207 50 621.6 0 50 208 62 621.6 0 62 209 333 621.6 0 333 210 345 621.6 0 345 211 342 621.6 0 342 212 80 621.6 0 80 213 149 621.6 0 149 214 96 621.6 0 96 215 169 621.6 0 169 216 45 621.6 0 45 217 39 621.6 0 39 218 44 621.6 0 44 219 54 621.6 0 54 220 41 621.6 0 41 221 38 621.6 0 38 222 47 621.6 0 47 223 60 621.6 0 60 224 71 621.6 0 71 225 59 621.6 0 59 226 40 621.6 0 40 227 35 621.6 0 35 228 51 621.6 0 51 229 50 621.6 0 50 230 76 621.6 0 76 231 67 621.6 0 67 232 71 621.6 0 71 233 210 621.6 0 210 234 251 621.6 0 251 235 346 621.6 0 346 236 130 621.6 0 130 237 54 621.6 0 54 238 53 621.6 0 53 239 61 621.6 0 61 240 96 621.6 0 96 241 83 621.6 0 83 242 100 621.6 0 100 243 98 621.6 0 98 244 117 621.6 0 117 245 82 621.6 0 82 246 100 621.6 0 100 247 75 621.6 0 75 248 129 621.6 0 129 249 193 621.6 0 193 250 224 621.6 0 224 251 140 621.6 0 140 252 120 621.6 0 120 253 1588 621.6 0 1588 254 1143 621.6 0 1143 255 1774 621.6 0 1774 256 117 621.6 0 117 257 76 621.6 0 76 258 68 621.6 0 68 259 65 621.6 0 65 260 95 621.6 0 95 261 94 621.6 0 94 262 90 621.6 0 90 263 63 621.6 0 63 264 69 621.6 0 69 265 63 621.6 0 63 266 80 621.6 0 80 267 70 621.6 0 70 268 88 621.6 0 88 269 70 621.6 0 70 270 110 621.6 0 110 271 110 621.6 0 110 272 156 621.6 0 156 273 745 621.6 0 745 274 605 621.6 0 605 275 476 621.6 0 476 276 73 621.6 0 73 277 51 621.6 0 51 278 88 621.6 0 88 279 95 621.6 0 95 280 100 621.6 0 100 281 83 621.6 0 83 282 82 621.6 0 82 283 92 621.6 0 92 284 99 621.6 0 99 285 121 621.6 0 121 286 91 621.6 0 91 287 107 621.6 0 107 288 97 621.6 0 97 289 112 621.6 0 112 290 76 621.6 0 76 291 75 621.6 0 75 292 169 621.6 0 169 293 130 621.6 0 130 294 176 621.6 0 176 295 119 621.6 0 119 296 148 621.6 0 148 297 153 621.6 0 153 298 170 621.6 0 170 299 177 621.6 0 177 300 366 621.6 0 366 301 278 621.6 0 278 Finished in 1.80 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 37,478 Reads with adapters: 7,579 (20.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 7,561 (20.2%) Total basepairs processed: 8,499,261 bp Total written (filtered): 2,068,591 bp (24.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7579 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 6 0.0 3 0 0 5 1 19 113 0.0 3 0 94 6 10 3 20 7367 0.0 4 6784 454 91 31 7 21 18 0.0 4 5 11 2 22 7 0.0 4 3 3 0 0 1 23 19 0.0 4 0 1 0 11 7 34 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 4 0.0 4 0 0 0 0 4 108 5 0.0 4 0 0 0 0 5 111 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 1 118 1 0.0 4 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 18 0.0 4 0 0 0 0 18 138 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 7,561 Reads with adapters: 646 (8.5%) Reads written (passing filters): 7,561 (100.0%) Total basepairs processed: 2,068,591 bp Total written (filtered): 2,060,971 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 646 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.6% C: 15.0% G: 76.9% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 40 118.1 0 40 4 10 29.5 0 10 9 6 0.0 0 0 6 10 17 0.0 1 1 16 11 486 0.0 1 26 460 12 76 0.0 1 3 73 13 3 0.0 1 0 3 14 2 0.0 1 0 2 15 1 0.0 1 0 1 152 2 0.0 1 2 191 2 0.0 1 0 2 210 1 0.0 1 1