This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.25 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 46,822 (1.8%) Reads that were too short: 8,568 (0.3%) Reads written (passing filters): 38,254 (1.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 8,733,520 bp (1.1%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 46822 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 251 621.6 0 251 7 8628 621.6 0 8628 8 202 621.6 0 202 9 7749 621.6 0 7749 10 38 621.6 0 38 11 33 621.6 0 33 12 23 621.6 0 23 13 28 621.6 0 28 14 25 621.6 0 25 15 32 621.6 0 32 16 50 621.6 0 50 17 37 621.6 0 37 18 16 621.6 0 16 19 20 621.6 0 20 20 21 621.6 0 21 21 28 621.6 0 28 22 41 621.6 0 41 23 36 621.6 0 36 24 32 621.6 0 32 25 31 621.6 0 31 26 97 621.6 0 97 27 50 621.6 0 50 28 110 621.6 0 110 29 38 621.6 0 38 30 49 621.6 0 49 31 59 621.6 0 59 32 26 621.6 0 26 33 26 621.6 0 26 34 36 621.6 0 36 35 31 621.6 0 31 36 18 621.6 0 18 37 39 621.6 0 39 38 30 621.6 0 30 39 43 621.6 0 43 40 36 621.6 0 36 41 13 621.6 0 13 42 55 621.6 0 55 43 51 621.6 0 51 44 29 621.6 0 29 45 27 621.6 0 27 46 26 621.6 0 26 47 19 621.6 0 19 48 25 621.6 0 25 49 42 621.6 0 42 50 33 621.6 0 33 51 57 621.6 0 57 52 54 621.6 0 54 53 43 621.6 0 43 54 29 621.6 0 29 55 35 621.6 0 35 56 44 621.6 0 44 57 26 621.6 0 26 58 42 621.6 0 42 59 41 621.6 0 41 60 44 621.6 0 44 61 31 621.6 0 31 62 39 621.6 0 39 63 37 621.6 0 37 64 22 621.6 0 22 65 35 621.6 0 35 66 32 621.6 0 32 67 57 621.6 0 57 68 71 621.6 0 71 69 628 621.6 0 628 70 177 621.6 0 177 71 704 621.6 0 704 72 39 621.6 0 39 73 47 621.6 0 47 74 25 621.6 0 25 75 49 621.6 0 49 76 53 621.6 0 53 77 56 621.6 0 56 78 65 621.6 0 65 79 71 621.6 0 71 80 39 621.6 0 39 81 54 621.6 0 54 82 43 621.6 0 43 83 56 621.6 0 56 84 52 621.6 0 52 85 39 621.6 0 39 86 47 621.6 0 47 87 34 621.6 0 34 88 36 621.6 0 36 89 52 621.6 0 52 90 46 621.6 0 46 91 46 621.6 0 46 92 47 621.6 0 47 93 41 621.6 0 41 94 37 621.6 0 37 95 30 621.6 0 30 96 53 621.6 0 53 97 60 621.6 0 60 98 66 621.6 0 66 99 69 621.6 0 69 100 51 621.6 0 51 101 52 621.6 0 52 102 44 621.6 0 44 103 28 621.6 0 28 104 32 621.6 0 32 105 47 621.6 0 47 106 35 621.6 0 35 107 62 621.6 0 62 108 46 621.6 0 46 109 56 621.6 0 56 110 40 621.6 0 40 111 46 621.6 0 46 112 73 621.6 0 73 113 39 621.6 0 39 114 85 621.6 0 85 115 84 621.6 0 84 116 107 621.6 0 107 117 39 621.6 0 39 118 46 621.6 0 46 119 35 621.6 0 35 120 38 621.6 0 38 121 30 621.6 0 30 122 42 621.6 0 42 123 33 621.6 0 33 124 52 621.6 0 52 125 29 621.6 0 29 126 77 621.6 0 77 127 93 621.6 0 93 128 56 621.6 0 56 129 63 621.6 0 63 130 95 621.6 0 95 131 69 621.6 0 69 132 284 621.6 0 284 133 85 621.6 0 85 134 254 621.6 0 254 135 84 621.6 0 84 136 86 621.6 0 86 137 129 621.6 0 129 138 302 621.6 0 302 139 350 621.6 0 350 140 2132 621.6 0 2132 141 3922 621.6 0 3922 142 1478 621.6 0 1478 143 455 621.6 0 455 144 1405 621.6 0 1405 145 274 621.6 0 274 146 39 621.6 0 39 147 35 621.6 0 35 148 101 621.6 0 101 149 273 621.6 0 273 150 247 621.6 0 247 151 361 621.6 0 361 152 211 621.6 0 211 153 401 621.6 0 401 154 37 621.6 0 37 155 31 621.6 0 31 156 40 621.6 0 40 157 45 621.6 0 45 158 34 621.6 0 34 159 28 621.6 0 28 160 33 621.6 0 33 161 29 621.6 0 29 162 22 621.6 0 22 163 21 621.6 0 21 164 26 621.6 0 26 165 29 621.6 0 29 166 22 621.6 0 22 167 29 621.6 0 29 168 22 621.6 0 22 169 41 621.6 0 41 170 28 621.6 0 28 171 16 621.6 0 16 172 34 621.6 0 34 173 52 621.6 0 52 174 37 621.6 0 37 175 41 621.6 0 41 176 37 621.6 0 37 177 24 621.6 0 24 178 34 621.6 0 34 179 35 621.6 0 35 180 28 621.6 0 28 181 32 621.6 0 32 182 35 621.6 0 35 183 31 621.6 0 31 184 39 621.6 0 39 185 45 621.6 0 45 186 48 621.6 0 48 187 30 621.6 0 30 188 51 621.6 0 51 189 57 621.6 0 57 190 68 621.6 0 68 191 100 621.6 0 100 192 43 621.6 0 43 193 20 621.6 0 20 194 42 621.6 0 42 195 28 621.6 0 28 196 19 621.6 0 19 197 24 621.6 0 24 198 26 621.6 0 26 199 42 621.6 0 42 200 24 621.6 0 24 201 34 621.6 0 34 202 32 621.6 0 32 203 38 621.6 0 38 204 38 621.6 0 38 205 29 621.6 0 29 206 31 621.6 0 31 207 33 621.6 0 33 208 28 621.6 0 28 209 318 621.6 0 318 210 97 621.6 0 97 211 86 621.6 0 86 212 37 621.6 0 37 213 35 621.6 0 35 214 36 621.6 0 36 215 44 621.6 0 44 216 25 621.6 0 25 217 35 621.6 0 35 218 36 621.6 0 36 219 27 621.6 0 27 220 26 621.6 0 26 221 28 621.6 0 28 222 46 621.6 0 46 223 27 621.6 0 27 224 30 621.6 0 30 225 26 621.6 0 26 226 31 621.6 0 31 227 23 621.6 0 23 228 25 621.6 0 25 229 35 621.6 0 35 230 24 621.6 0 24 231 23 621.6 0 23 232 36 621.6 0 36 233 59 621.6 0 59 234 58 621.6 0 58 235 101 621.6 0 101 236 59 621.6 0 59 237 40 621.6 0 40 238 45 621.6 0 45 239 24 621.6 0 24 240 56 621.6 0 56 241 29 621.6 0 29 242 48 621.6 0 48 243 46 621.6 0 46 244 38 621.6 0 38 245 38 621.6 0 38 246 50 621.6 0 50 247 71 621.6 0 71 248 84 621.6 0 84 249 164 621.6 0 164 250 232 621.6 0 232 251 137 621.6 0 137 252 51 621.6 0 51 253 668 621.6 0 668 254 554 621.6 0 554 255 714 621.6 0 714 256 54 621.6 0 54 257 48 621.6 0 48 258 56 621.6 0 56 259 46 621.6 0 46 260 39 621.6 0 39 261 33 621.6 0 33 262 41 621.6 0 41 263 32 621.6 0 32 264 22 621.6 0 22 265 44 621.6 0 44 266 35 621.6 0 35 267 34 621.6 0 34 268 42 621.6 0 42 269 42 621.6 0 42 270 55 621.6 0 55 271 59 621.6 0 59 272 94 621.6 0 94 273 334 621.6 0 334 274 296 621.6 0 296 275 215 621.6 0 215 276 43 621.6 0 43 277 36 621.6 0 36 278 57 621.6 0 57 279 50 621.6 0 50 280 55 621.6 0 55 281 52 621.6 0 52 282 39 621.6 0 39 283 53 621.6 0 53 284 97 621.6 0 97 285 58 621.6 0 58 286 72 621.6 0 72 287 84 621.6 0 84 288 95 621.6 0 95 289 119 621.6 0 119 290 48 621.6 0 48 291 70 621.6 0 70 292 113 621.6 0 113 293 80 621.6 0 80 294 74 621.6 0 74 295 103 621.6 0 103 296 101 621.6 0 101 297 90 621.6 0 90 298 126 621.6 0 126 299 119 621.6 0 119 300 177 621.6 0 177 301 185 621.6 0 185 Finished in 1.62 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 38,254 Reads with adapters: 8,177 (21.4%) Reads that were too short: 29 (0.1%) Reads written (passing filters): 8,148 (21.3%) Total basepairs processed: 8,733,520 bp Total written (filtered): 2,216,480 bp (25.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8177 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 63 0.0 3 0 63 17 7891 0.0 3 7750 135 4 2 18 17 0.0 3 2 15 19 6 0.0 3 2 0 4 20 6 0.0 3 1 0 0 5 21 1 0.0 3 1 29 1 0.0 3 0 0 0 1 34 125 0.0 3 0 0 2 123 37 15 0.0 3 0 0 0 15 43 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 63 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 80 7 0.0 3 0 0 0 7 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 14 0.0 3 0 0 0 14 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 8,148 Reads with adapters: 508 (6.2%) Reads written (passing filters): 8,148 (100.0%) Total basepairs processed: 2,216,480 bp Total written (filtered): 2,214,946 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 508 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.5% C: 5.3% G: 37.8% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 498 127.3 0 498 4 10 31.8 0 10